FastQCFastQC Report
Wed 25 May 2016
SRR1780377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181437
Sequences flagged as poor quality0
Sequence length50
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACAAC250.002347533435.223
TCCTGCG300.00567834429.39011819
TGATAGA300.005701416629.36575512
CGATGGC300.00573229529.33333210
CTATACA1001.8189894E-1228.6078951
CTTTACA2150.027.6355361
CTTATAT406.997614E-427.5075951
TGCGTTT407.054301E-427.46967133
TTTACAC2200.026.9702222
TTACACA2450.025.1151283
AGTTCAG450.001389156724.45794740
CGTATTT450.001402628824.4174862
CTTATAC10750.024.3602121
TTATACA9650.024.1391532
GATGGCT551.5863382E-424.011
CTCATCT551.6012577E-423.9669237
ACGAGAG952.591878E-823.20914521
TATACAC9950.023.1904753
AGTATGC1153.4924597E-1022.93752928
TCTCGGG1355.456968E-1222.78965443
Produced by FastQC (version 0.11.5)