FastQCFastQC Report
Wed 25 May 2016
SRR1780377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181437
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3939521.712770824032585No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT3087817.01857945182074RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC104475.757921482387826No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC53802.9652165765527427No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG43992.424533033504743No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT28391.5647304574039473RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC22991.267106488753672No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC17270.9518455441833804No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG13620.7506737875956944No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG11650.642096154588094No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9370.5164327011579777No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC9150.5043072802129664No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC7040.3880134702403589No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG6290.3466768079278207No Hit
CTTATTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5360.29541934666027325No Hit
CTTATCACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC4590.25298037335273404No Hit
CTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT4200.23148530895021413RNA PCR Primer, Index 17 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3900.21495064402519884No Hit
ACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTTCT3520.19400673512017946RNA PCR Primer, Index 17 (95% over 24bp)
TCTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT2930.16148856076764936No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGT2490.13723771887762695No Hit
CTTATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC2150.1184984319626096No Hit
ATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTTCTGCTT2130.1173961209676086Illumina PCR Primer Index 9 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACAAATACAGATATCGTATGCCGTCTT2040.112435721490104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAC254.4220847E-544.0099261
TCTTTAT1900.044.0099261
CGGTCGT254.4220847E-544.00992610
TATTGTG207.840875E-443.997795
CCGGTCG207.840875E-443.997799
CCGTCTT32500.042.77939644
CTTTACA10200.042.499781
TTTACAC10200.042.4880642
CTTATAA1100.040.0090261
CTATACA6000.039.975681
GCCGTCT35200.039.43552443
ATTACAC800.038.498074
TTACACA11750.036.8832553
GGTCGTG301.2950573E-436.6749411
CTAAGGG301.2971609E-436.6648336
TACGGAG301.2971609E-436.664834
CTACGGA301.2971609E-436.664833
AATTACC301.2971609E-436.664835
ATAACAC1200.036.664834
GAGGCTC301.2971609E-436.664838
Produced by FastQC (version 0.11.5)