FastQCFastQC Report
Wed 25 May 2016
SRR1780376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51958
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTGT150.00221389969.992314
ACTTACC200.00691792752.49422537
ATGTTAC200.00691792752.49422544
GAGATAA200.00691792752.49422516
TTAGTTA305.830596E-446.66153320
AAAGCAA400.002310145435.26771
TTGTTGT400.002399598734.9961511
TCTCGGT450.00427336431.10768744
GACGAAC657.8620017E-426.92011520
CATACAC950.007125133618.4190273
ACACCTC1300.002253141516.152078
TGCCGAC2851.9464132E-512.27935170
CTGCCGA3104.601726E-511.28908269
GCTGCCG3155.417015E-511.10988868
CCGACGA2350.00906205310.42438518
CGCTGCC3501.5773127E-49.99889967
ACGCTGC3753.1589766E-49.33230766
GACGCTG4107.703587E-48.53564665
TGACGCT4400.00155070317.95367164
TCTGACG4650.00267061787.52605462
Produced by FastQC (version 0.11.5)