FastQCFastQC Report
Wed 25 May 2016
SRR1780376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51958
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT36046.936371684822356No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT33886.520651295276954No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC11402.1940798337118443No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC5841.12398475691905No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG3900.7506062589014204No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT3080.5927864813888141No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1990.3830016551830324No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1570.3021671349936487No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT1470.2829208206628431No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1270.24442819200123175No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1110.2136340890719427No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGT1070.20593556333962046No Hit
CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1060.2040109319065399No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGTCTT1000.1924631433080565No Hit
CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC1000.1924631433080565No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGT990.19053851187497595No Hit
TTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC780.15012125178028407No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGTCTT730.14049809461488125No Hit
CTTATTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG690.13279956888255898No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTCGTTATCTCGTATGCCGT620.11932714885099502No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGTCTT520.1000808345201894No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGGA150.00221293370.000012
ATAACAC301.00515535E-770.000014
TTGGAGA150.00221293370.000014
TCTTTAT150.00221293370.000011
AATTTGT207.8576886E-570.026
CAATTTG207.8576886E-570.025
TTTACAC2000.064.752
CTTTACA2050.063.170731
CTTATAA401.0408257E-861.251
CTACACA352.9206058E-760.04
TATCCGA252.3697758E-455.99999611
ATCCGAG252.3697758E-455.99999612
ATATCCG252.3697758E-455.99999610
CTATACA850.053.529411
TTACACA2450.052.8571433
TGAGACT200.00691491952.512
ATGGTAC200.00691491952.523
CAATCAA200.00691491952.59
CGAGGCT200.00691491952.551
TTCCATC200.00691491952.561
Produced by FastQC (version 0.11.5)