##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780376_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 51958 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.905750798722046 34.0 34.0 34.0 31.0 34.0 2 33.364467454482465 34.0 34.0 34.0 33.0 34.0 3 33.496092998190846 34.0 34.0 34.0 34.0 34.0 4 33.51753339235536 34.0 34.0 34.0 34.0 34.0 5 33.54087917163863 34.0 34.0 34.0 34.0 34.0 6 37.31633242234112 38.0 38.0 38.0 37.0 38.0 7 36.89183571346087 38.0 37.0 38.0 35.0 38.0 8 36.9330228261288 38.0 37.0 38.0 35.0 38.0 9 36.670637822856925 38.0 37.0 38.0 34.0 38.0 10-11 36.361878825204975 38.0 37.0 38.0 33.5 38.0 12-13 36.21837830555449 38.0 37.0 38.0 34.0 38.0 14-15 36.365352784941685 38.0 37.0 38.0 34.0 38.0 16-17 36.428836752761846 38.0 37.0 38.0 34.0 38.0 18-19 36.48326532968936 38.0 37.0 38.0 34.0 38.0 20-21 36.266859771353786 38.0 37.0 38.0 33.5 38.0 22-23 36.31705415912853 38.0 37.0 38.0 33.0 38.0 24-25 35.57035490203626 38.0 36.0 38.0 31.0 38.0 26-27 35.760306401324144 38.0 36.0 38.0 31.0 38.0 28-29 35.67788406020247 38.0 36.0 38.0 31.0 38.0 30-31 35.33734939759036 38.0 36.0 38.0 29.0 38.0 32-33 35.714192232187536 38.0 36.5 38.0 31.5 38.0 34-35 35.426450209784825 38.0 36.0 38.0 31.0 38.0 36-37 35.44686092613264 38.0 36.0 38.0 31.0 38.0 38-39 34.82671580892259 37.5 35.0 38.0 27.0 38.0 40-41 34.89233611763348 37.0 35.0 38.0 29.0 38.0 42-43 35.57956426344355 37.5 36.0 38.0 32.0 38.0 44-45 35.82421378805959 38.0 36.5 38.0 31.5 38.0 46-47 36.03154470918819 38.0 37.0 38.0 32.5 38.0 48-49 35.6938969937257 38.0 36.5 38.0 31.5 38.0 50-51 35.83717618076139 38.0 36.5 38.0 31.5 38.0 52-53 35.69142384233419 38.0 36.0 38.0 31.0 38.0 54-55 35.909831017360176 38.0 37.0 38.0 32.5 38.0 56-57 35.901574348512256 38.0 37.0 38.0 32.5 38.0 58-59 35.89333692597867 38.0 37.0 38.0 32.0 38.0 60-61 35.63299203202587 38.0 36.5 38.0 31.5 38.0 62-63 36.052725278109236 38.0 37.0 38.0 33.0 38.0 64-65 36.031159782901575 38.0 37.0 38.0 33.0 38.0 66-67 35.025029831787215 38.0 35.5 38.0 29.0 38.0 68-69 33.2520304861619 37.0 33.0 38.0 21.5 38.0 70-71 32.08010316024482 37.0 29.0 38.0 16.0 38.0 72-73 30.875485969436852 37.0 24.5 38.0 11.0 38.0 74-75 31.133915855113745 37.0 25.0 38.0 11.0 38.0 76 30.879672042803804 37.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14663093815260453 1101 2 0.41360723764522334 1101 3 0.383280905011695 1101 4 0.4041318106405001 1101 5 0.4072046953275432 1101 6 -1.523048389363879 1101 7 0.01982245905514901 1101 8 0.6236462468205914 1101 9 0.840424514982054 1101 10-11 0.3022967173943414 1101 12-13 0.44084527693003395 1101 14-15 0.5043137902314783 1101 16-17 -0.004805910240314404 1101 18-19 -0.3464378454796133 1101 20-21 0.19729945631871004 1101 22-23 1.0658908261195492 1101 24-25 -1.21762108081208 1101 26-27 0.4439723215223168 1101 28-29 0.9925569803501801 1101 30-31 1.4201004009342668 1101 32-33 0.5610683161641745 1101 34-35 0.011023448087108534 1101 36-37 0.13934142367428848 1101 38-39 0.04917094421490731 1101 40-41 0.6038372892831418 1101 42-43 0.5469302261178441 1101 44-45 0.18139929903120589 1101 46-47 0.8177588549258985 1101 48-49 -1.1357070212071392 1101 50-51 -1.3170138433697929 1101 52-53 -1.2842853528728497 1101 54-55 -0.3682662074727787 1101 56-57 -1.1904272405418226 1101 58-59 -1.1072085356149444 1101 60-61 -1.1861565682109685 1101 62-63 -0.7000957088607791 1101 64-65 -1.0624127075842225 1101 66-67 -1.4020967347390965 1101 68-69 -1.013876446331686 1101 70-71 -0.3066249863182051 1101 72-73 -1.9691346452659886 1101 74-75 -0.22012675846904983 1101 76 0.17808414306503906 1102 1 -0.9825093014527475 1102 2 -0.05009959815463816 1102 3 0.23476082044509639 1102 4 0.4834129945728449 1102 5 -0.2880031975547723 1102 6 0.308346959473333 1102 7 -0.4010725374212427 1102 8 0.6491924060876855 1102 9 0.9145964670609814 1102 10-11 0.6371275842019486 1102 12-13 0.643276566570627 1102 14-15 -0.3331597827072059 1102 16-17 0.0581785858837236 1102 18-19 -0.6832066967833512 1102 20-21 0.11255667971547467 1102 22-23 0.32602119962201925 1102 24-25 -5.107332144941751 1102 26-27 -1.5338289469766266 1102 28-29 -0.631914478423603 1102 30-31 1.573201176128073 1102 32-33 -0.13008742731715728 1102 34-35 0.7139832789539184 1102 36-37 -2.9624027623722213 1102 38-39 -0.3925133404926271 1102 40-41 0.5534496923839214 1102 42-43 0.4154115509945129 1102 44-45 0.5377241757049163 1102 46-47 0.954827212924485 1102 48-49 -3.942965654047171 1102 50-51 -0.6122041182112312 1102 52-53 -0.9974636474464873 1102 54-55 0.41327008569142265 1102 56-57 0.3849779743982751 1102 58-59 -0.6862254489341808 1102 60-61 -5.827893707041131 1102 62-63 -2.460754623589466 1102 64-65 -0.4012076335884487 1102 66-67 0.5587031243165725 1102 68-69 -2.453006115112519 1102 70-71 -1.6688519066846652 1102 72-73 -4.853560297133498 1102 74-75 -2.3056623469116175 1102 76 -0.8101117695494757 1103 1 0.8958329445365578 1103 2 0.6118136519939057 1103 3 -0.5045439923891308 1103 4 0.18008560301016274 1103 5 0.20367832353851156 1103 6 0.41128813594392 1103 7 0.8860635291265879 1103 8 0.23133738529148928 1103 9 -0.15091155432939019 1103 10-11 -0.3082823979727394 1103 12-13 -0.998583207642092 1103 14-15 -0.8837391053453558 1103 16-17 0.011609958432735823 1103 18-19 0.6886738736266338 1103 20-21 0.050316632181306886 1103 22-23 0.15215013604507277 1103 24-25 -3.260332739118809 1103 26-27 -0.12503512641809778 1103 28-29 0.13999580083962115 1103 30-31 1.264377646716305 1103 32-33 1.4770129886067949 1103 34-35 0.6144586206057312 1103 36-37 -0.5726968800192793 1103 38-39 0.4189839857105824 1103 40-41 -0.3419751442173862 1103 42-43 -0.24969540087714392 1103 44-45 1.1440075981648121 1103 46-47 0.9566097441187793 1103 48-49 -1.7400541864298233 1103 50-51 0.08350295249583439 1103 52-53 0.25743831333782197 1103 54-55 -0.42838172654862205 1103 56-57 0.28099698806434503 1103 58-59 -0.7179396497660235 1103 60-61 -4.65461682052895 1103 62-63 -0.9403297869881584 1103 64-65 0.2887804828702514 1103 66-67 -0.6105630670084352 1103 68-69 0.08598675476270756 1103 70-71 0.2586584914139678 1103 72-73 -4.034190124822153 1103 74-75 -1.1274834996151242 1103 76 -2.112993528313588 1104 1 0.4225177906604358 1104 2 0.3129347185220652 1104 3 0.18393802293356032 1104 4 -0.37460426410666514 1104 5 -0.356335527476503 1104 6 -0.08955591028384191 1104 7 0.604195373475811 1104 8 0.37902383293897657 1104 9 1.11944294508627 1104 10-11 -0.8051009929604049 1104 12-13 0.7584180476061491 1104 14-15 0.7713706206642499 1104 16-17 0.5965339942722423 1104 18-19 1.002399363822711 1104 20-21 0.2224804204339179 1104 22-23 0.4829296884841554 1104 24-25 0.3525313910807313 1104 26-27 1.065308119047856 1104 28-29 1.5014640084317108 1104 30-31 1.1068656352892177 1104 32-33 0.5751604153479093 1104 34-35 -0.5112826251199323 1104 36-37 0.9738555157734723 1104 38-39 1.2453052426901934 1104 40-41 1.9055468226267465 1104 42-43 0.28107982311974666 1104 44-45 0.49104045104105865 1104 46-47 1.0750359225371682 1104 48-49 -0.10872925027434377 1104 50-51 0.6515677059910558 1104 52-53 0.44845246734379174 1104 54-55 -0.1252609197270047 1104 56-57 -0.19378983615762024 1104 58-59 -0.8135332956128849 1104 60-61 0.9571002724892708 1104 62-63 0.5803601666975098 1104 64-65 0.831935051540988 1104 66-67 -0.2516269980996455 1104 68-69 0.7681708866936248 1104 70-71 -0.16403553100735735 1104 72-73 -1.0461149830656673 1104 74-75 -0.8258560053503103 1104 76 -1.0155602945344242 1105 1 0.6635945946511512 1105 2 0.24860170054665787 1105 3 0.22934956503384285 1105 4 -0.2525177413578916 1105 5 0.036672127120553455 1105 6 -0.015246114119740639 1105 7 0.6411352547865548 1105 8 -0.2306777237824491 1105 9 0.9308302332947491 1105 10-11 0.36061243268679277 1105 12-13 -0.9638662905722342 1105 14-15 0.06456749002006035 1105 16-17 -0.24431059160546198 1105 18-19 0.6907110521343967 1105 20-21 0.29870386586265596 1105 22-23 0.14907314767396684 1105 24-25 0.4424514048418047 1105 26-27 0.10145243830475437 1105 28-29 0.7939513224422043 1105 30-31 1.2799111328380235 1105 32-33 0.9889601917304631 1105 34-35 -0.7914279764573422 1105 36-37 0.32818165321219794 1105 38-39 -0.35138779936708886 1105 40-41 -0.1759875370533095 1105 42-43 -0.31348455290158483 1105 44-45 0.8586116215923667 1105 46-47 0.7491995072967796 1105 48-49 -0.4050868661683822 1105 50-51 0.8504582194510988 1105 52-53 0.22223332225047443 1105 54-55 0.656776579197917 1105 56-57 0.26051909993940114 1105 58-59 0.4279520402433121 1105 60-61 0.7646387604913372 1105 62-63 -0.4569667448015835 1105 64-65 0.6085326261518063 1105 66-67 0.8709325615460131 1105 68-69 -0.04878533589173628 1105 70-71 0.42855069071793395 1105 72-73 -0.02996722354042447 1105 74-75 -0.2899697062189759 1105 76 -0.37829358773129584 1106 1 -0.7335345919517238 1106 2 0.08318816671207685 1106 3 0.28198114398121277 1106 4 0.11556154777402838 1106 5 -0.2746744210680987 1106 6 0.0032091148618036414 1106 7 -1.058454629013859 1106 8 0.16371735482315586 1106 9 -0.7506461850237756 1106 10-11 0.5173109972800987 1106 12-13 1.3966942015672075 1106 14-15 -0.43474898298061504 1106 16-17 -0.08060587526916407 1106 18-19 0.6076625217174509 1106 20-21 0.7455596416657997 1106 22-23 0.2008503178721881 1106 24-25 -1.0390933661766582 1106 26-27 0.8405160063157027 1106 28-29 0.6085446238776129 1106 30-31 0.4008954117170376 1106 32-33 0.5725213673969094 1106 34-35 1.3898775601113513 1106 36-37 1.324251372966124 1106 38-39 0.13135314390017072 1106 40-41 0.9531990658175076 1106 42-43 0.6034669166814624 1106 44-45 0.7907715669102018 1106 46-47 1.135734025410244 1106 48-49 1.213334186463193 1106 50-51 -0.03094984616748775 1106 52-53 0.5125614152101505 1106 54-55 0.43411765917057465 1106 56-57 0.4243946980255231 1106 58-59 0.7719370026493237 1106 60-61 0.8618704761441052 1106 62-63 0.6329170556085373 1106 64-65 -1.1611187750785348 1106 66-67 0.47209455744485496 1106 68-69 2.8273253954821485 1106 70-71 0.8205534248259312 1106 72-73 0.19884343948076477 1106 74-75 -0.3834420233755047 1106 76 0.308992810081417 1107 1 0.9038543349108892 1107 2 -0.1150346630897019 1107 3 0.07350540918968562 1107 4 0.007222518382370424 1107 5 0.030178620627047792 1107 6 0.30942921055558514 1107 7 0.8099664236177233 1107 8 0.04854305543832993 1107 9 -0.4782606757961574 1107 10-11 0.7339890560634217 1107 12-13 -0.11646369316963501 1107 14-15 0.1652168406694159 1107 16-17 0.3823127850179233 1107 18-19 0.5543474157707635 1107 20-21 0.6168856840444761 1107 22-23 0.04193029053110564 1107 24-25 0.47816569055608227 1107 26-27 0.043010879863196294 1107 28-29 0.4238214523123318 1107 30-31 0.6727682756951694 1107 32-33 1.122077074847347 1107 34-35 0.3124681274387626 1107 36-37 1.484025809056348 1107 38-39 0.7914693434900499 1107 40-41 0.46362285255708 1107 42-43 0.7579440185269846 1107 44-45 0.8066635696443072 1107 46-47 0.9634852215824949 1107 48-49 1.1306274195459025 1107 50-51 1.0842244532173382 1107 52-53 0.6280774780946317 1107 54-55 0.5074259298472654 1107 56-57 0.8222074116277156 1107 58-59 0.6324974947887654 1107 60-61 1.060093305945884 1107 62-63 0.8677085798737423 1107 64-65 0.8974936651128473 1107 66-67 0.17937411998757824 1107 68-69 0.8538120667056575 1107 70-71 0.12335588552313226 1107 72-73 1.1031496595764594 1107 74-75 -1.4523073685566388 1107 76 0.4528752434375356 1108 1 -0.6876648841251125 1108 2 0.2476429277758072 1108 3 -0.0956744042854325 1108 4 0.4048081542230406 1108 5 0.16257642416579188 1108 6 0.14783241808630265 1108 7 0.06643813982098123 1108 8 -0.5364390765236635 1108 9 -0.05260914611778844 1108 10-11 0.1981658188978983 1108 12-13 0.8981575915858784 1108 14-15 0.5011141648217716 1108 16-17 -0.3766648145637035 1108 18-19 0.5023993638227111 1108 20-21 0.8355076660892777 1108 22-23 0.10111271884105832 1108 24-25 1.1093650281312648 1108 26-27 -0.09829915238643139 1108 28-29 -0.14414246296701805 1108 30-31 1.1028432342936156 1108 32-33 1.383996363612276 1108 34-35 0.7555736796047157 1108 36-37 1.4310536134667018 1108 38-39 1.5468297548907373 1108 40-41 1.1630693791848117 1108 42-43 0.6113389457474128 1108 44-45 1.0051077429206927 1108 46-47 0.7067084413157829 1108 48-49 1.173772194234303 1108 50-51 1.0570301709894991 1108 52-53 -0.3755397100863078 1108 54-55 -0.4448751248027847 1108 56-57 -0.1678997966003024 1108 58-59 0.18226651771215074 1108 60-61 1.2798701733737516 1108 62-63 0.9405317298780957 1108 64-65 0.7789326958807408 1108 66-67 0.9927838646053644 1108 68-69 0.3881317738441652 1108 70-71 -1.6987481849030885 1108 72-73 -0.3838633274365293 1108 74-75 -1.3584130078837546 1108 76 -0.19067918870749878 1109 1 -0.6912413588690143 1109 2 0.2100678659601911 1109 3 0.19927437706391515 1109 4 0.2762758334883202 1109 5 -0.08764434588012904 1109 6 -0.1960390054389478 1109 7 -0.16841703502889516 1109 8 0.39022111422165295 1109 9 0.8452184766303574 1109 10-11 0.7903373130695712 1109 12-13 0.88071675795819 1109 14-15 0.9581081598765238 1109 16-17 0.984713673603018 1109 18-19 0.6964783111911359 1109 20-21 1.199976934898885 1109 22-23 0.8296097163684308 1109 24-25 -0.16973246392100805 1109 26-27 0.3954972093232172 1109 28-29 -0.8219065039419391 1109 30-31 0.6466660884087716 1109 32-33 0.7642506907051754 1109 34-35 0.2066068834196244 1109 36-37 1.5869393428909362 1109 38-39 1.9264659258550552 1109 40-41 1.1016953064190105 1109 42-43 1.2524330820949885 1109 44-45 0.9759219428500572 1109 46-47 0.9167490630042323 1109 48-49 0.7889920812000426 1109 50-51 0.4281666951858938 1109 52-53 -0.15426189306052862 1109 54-55 -0.24492369421287918 1109 56-57 0.5024022168225173 1109 58-59 0.36122271693503905 1109 60-61 1.2100847618583899 1109 62-63 0.7212629202701848 1109 64-65 1.3036368640192038 1109 66-67 0.704237414587709 1109 68-69 -0.5307573112847663 1109 70-71 0.8496887631717982 1109 72-73 0.7082419357213681 1109 74-75 -0.03193826397700761 1109 76 0.20842890030698413 1110 1 -0.7632391349994805 1110 2 -0.04817898355796757 1110 3 0.029148357790383272 1110 4 0.23522690835859095 1110 5 0.46359731460911746 1110 6 0.1545910909287329 1110 7 0.688236041323961 1110 8 0.7993204470607935 1110 9 1.014788528520647 1110 10-11 -0.7562249552773466 1110 12-13 0.6013431478766407 1110 14-15 0.9301244682530978 1110 16-17 0.59280299902926 1110 18-19 0.06225851875228727 1110 20-21 1.1716689289685647 1110 22-23 -0.6104384674714467 1110 24-25 -0.651292879043325 1110 26-27 1.0885568831556824 1110 28-29 0.4125264093271497 1110 30-31 -0.24487394124282247 1110 32-33 0.7323752273990252 1110 34-35 1.4565142221890852 1110 36-37 1.6168225897404511 1110 38-39 1.2465178161441628 1110 40-41 -0.16720758461138274 1110 42-43 0.4690287084849132 1110 44-45 1.1261257975572434 1110 46-47 0.9133205974742111 1110 48-49 1.5984342605921782 1110 50-51 -0.05186480963249096 1110 52-53 0.716151376027689 1110 54-55 0.10501144292573628 1110 56-57 1.0147261604844857 1110 58-59 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0.16237345129193415 2116 50-51 -0.6942748252819371 2116 52-53 0.37952047953763923 2116 54-55 -0.6790820066606713 2116 56-57 -0.007518418098115376 2116 58-59 -2.6514130391217705 2116 60-61 -2.5337017878492105 2116 62-63 0.04122950339466769 2116 64-65 -2.3027227851035974 2116 66-67 -0.21023626962281838 2116 68-69 -0.22447076157716594 2116 70-71 1.1998349620022069 2116 72-73 -0.5386252321984344 2116 74-75 -0.229724395973669 2116 76 -0.707297916735623 2117 1 0.8811270621836158 2117 2 0.38029812911808847 2117 3 0.28850135074812755 2117 4 -0.26114192966957717 2117 5 -0.15590092482749895 2117 6 0.2328261261399973 2117 7 0.6046092807605774 2117 8 0.5028855879058653 2117 9 -1.1408012602117452 2117 10-11 -0.5953534764041137 2117 12-13 -1.8162404788839197 2117 14-15 -0.28983591907084616 2117 16-17 -1.626717247449612 2117 18-19 -2.005413136177289 2117 20-21 -0.25273688089058766 2117 22-23 0.02985642689474588 2117 24-25 -1.7737620691841727 2117 26-27 -2.6990751590978377 2117 28-29 -1.502416372362994 2117 30-31 -0.4958092405385983 2117 32-33 -1.8862427303474547 2117 34-35 0.07667267289330937 2117 36-37 -1.3022465123722213 2117 38-39 -0.144052166250205 2117 40-41 -0.8033211409494143 2117 42-43 0.08302518735815312 2117 44-45 -0.4124415439920526 2117 46-47 -1.2150354764694526 2117 48-49 -0.8699306161683822 2117 50-51 -0.17257343639305134 2117 52-53 0.8983696858868413 2117 54-55 -0.806190141581304 2117 56-57 -0.1968165331774827 2117 58-59 -1.9342131383281185 2117 60-61 -0.9955073434047677 2117 62-63 -0.6782025402561302 2117 64-65 -1.5240769517702688 2117 66-67 -1.0696112696228184 2117 68-69 -0.4150089560216088 2117 70-71 -0.15606781577557172 2117 72-73 1.1356679225634707 2117 74-75 0.7246406833914136 2117 76 1.2339223213596142 2118 1 -0.22802786739384828 2118 2 0.21238439672372067 2118 3 -0.506062909815256 2118 4 0.17888888018957516 2118 5 0.4002170329189809 2118 6 0.2813516543090131 2118 7 0.8149966047042412 2118 8 0.22889791184952912 2118 9 -3.309155133451185 2118 10-11 -1.6153798848548178 2118 12-13 -0.9761216408557516 2118 14-15 0.2681526372671854 2118 16-17 -3.467056155900316 2118 18-19 -0.6173189460364412 2118 20-21 -1.335373324552556 2118 22-23 -0.05057931254187764 2118 24-25 0.5247634589848431 2118 26-27 -3.7177811450133333 2118 28-29 -1.3022623230672181 2118 30-31 -1.7202260539188785 2118 32-33 -0.7183289979530869 2118 34-35 -0.2019364820362668 2118 36-37 -4.203599945470813 2118 38-39 0.22539105558078631 2118 40-41 -1.1073484296818137 2118 42-43 -0.07322481264184688 2118 44-45 -2.694296737654021 2118 46-47 -0.1183695433708607 2118 48-49 0.010406091578097687 2118 50-51 -1.6469352321676993 2118 52-53 -1.8718767929863915 2118 54-55 -1.7858336274967925 2118 56-57 -0.13668229021973843 2118 58-59 -1.6273799517084058 2118 60-61 -2.2547503011512475 2118 62-63 -2.449604388847682 2118 64-65 -0.23347395881252453 2118 66-67 -0.05882781891859423 2118 68-69 -1.7745490088385125 2118 70-71 -0.7434357735220516 2118 72-73 0.7569817429860066 2118 74-75 0.40245758479986193 2118 76 0.5448818284018664 2119 1 0.6857489109231096 2119 2 0.03011430558867545 2119 3 -0.07317144336951742 2119 4 -0.12990505992167556 2119 5 -0.32199861390312634 2119 6 -0.3674233486499219 2119 7 -1.1522184503318584 2119 8 0.36672487362015005 2119 9 -0.4114157560100651 2119 10-11 -0.11780620749654958 2119 12-13 -0.248816634346106 2119 14-15 0.11345977420646847 2119 16-17 -0.22368415921432216 2119 18-19 -0.23289475382434688 2119 20-21 -1.4669569439157968 2119 22-23 -0.19145397226491667 2119 24-25 1.224727951824235 2119 26-27 1.0943859753559408 2119 28-29 -0.3483328639596337 2119 30-31 0.7447698035790467 2119 32-33 0.6950335301567492 2119 34-35 0.4692672107084377 2119 36-37 -0.06009183800247797 2119 38-39 -1.4913801599476812 2119 40-41 -0.5529928846468906 2119 42-43 0.8553466159295837 2119 44-45 0.8989095589491214 2119 46-47 0.3137526012616405 2119 48-49 0.029787083411456194 2119 50-51 0.9551182652876236 2119 52-53 0.09637388756751619 2119 54-55 0.17339231640188757 2119 56-57 -1.322177615110256 2119 58-59 0.20316212427692903 2119 60-61 0.5956166061750636 2119 62-63 0.014385432432945322 2119 64-65 -0.21728861143413525 2119 66-67 0.9515284362595366 2119 68-69 0.6882276511212524 2119 70-71 0.1153344951488009 2119 72-73 1.183363563319773 2119 74-75 -0.04111561912959516 2119 76 -0.036429569396688066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 2.0 22 5.0 23 10.0 24 41.0 25 82.0 26 151.0 27 302.0 28 544.0 29 868.0 30 1411.0 31 2060.0 32 3064.0 33 4542.0 34 6435.0 35 7792.0 36 9391.0 37 15256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.95030601639786 24.49093498595019 11.5766580699796 34.98210092767235 2 13.93625620693637 15.974440894568689 50.69864121020825 19.39066168828669 3 13.120212479310212 28.176604180299474 39.195119134685704 19.50806420570461 4 9.43646791639401 25.8266292005081 35.53447014896648 29.202432734131413 5 8.666615343161785 41.65287347472959 38.1962354209169 11.484275761191732 6 18.59578890642442 41.22752992801878 16.282381923861582 23.894299241695215 7 18.947996458678162 38.45606066438277 18.62658300935371 23.969359867585357 8 16.902113245313522 37.9383348088841 24.01362639054621 21.145925555256166 9 16.803957042226415 19.814080603564417 16.934831979675895 46.44713037453327 10-11 17.595942876939066 25.472497016821276 31.99603525924785 24.935524846991804 12-13 21.683860040802188 17.857692751838023 28.354632587859424 32.10381461950036 14-15 23.040725201123983 22.59324839293275 19.34447053389276 35.021555872050506 16-17 25.33969744793872 27.572269910312176 18.447592286077217 28.64044035567189 18-19 22.817467954886638 25.88821740636668 19.13853497055314 32.15577966819354 20-21 20.50598560375688 23.043612148273606 19.067323607529158 37.38307864044035 22-23 23.39870664767697 30.241733707994918 19.596597251626314 26.7629623927018 24-25 24.844104853920474 32.99973055159937 19.198198544978638 22.957966049501522 26-27 22.36999114669541 38.32903498979945 19.454174525578352 19.846799337926786 28-29 16.747180414950538 31.525462873859656 29.631625543708378 22.09573116748143 30-31 20.44054813503214 27.465452865776204 30.152238346356675 21.941760652834983 32-33 18.11078178528812 20.861080103160244 36.85572962777628 24.172408483775357 34-35 21.96774317718157 24.349474575618768 35.36702721428846 18.315755032911195 36-37 16.739481889218215 27.092074367758574 34.687632318411026 21.480811424612188 38-39 19.947842488163516 22.367104199545786 29.16682705261942 28.518226259671277 40-41 18.365795450171294 23.816351668655454 31.910389160475766 25.90746372069749 42-43 25.74868162746834 22.012972015858963 33.599253243003965 18.639093113668732 44-45 18.715116055275416 25.410908810962702 29.443973978983024 26.43000115477886 46-47 26.12398475691905 19.729396820508875 26.922706801647482 27.223911620924596 48-49 22.967589206666926 18.984564455906693 28.438354055198428 29.609492282227954 50-51 16.525847800146273 19.195311597829015 34.379691289118135 29.89914931290658 52-53 18.86812425420532 19.45994841987759 40.032333808075755 21.639593517841334 54-55 16.96177681973902 19.587936410177452 35.24673774972093 28.2035490203626 56-57 24.434158358674313 19.27710843373494 30.413025905539087 25.87570730205166 58-59 17.852881173255323 28.563455098348665 34.247854035952116 19.3358096924439 60-61 20.357788983409677 38.54940528888718 22.84633742638285 18.2464683013203 62-63 14.50883405827784 46.80511182108626 20.115285422841524 18.570768697794374 64-65 14.218214711882673 48.15235382424265 19.34447053389276 18.284960929981906 66-67 14.00458062281073 47.37480272527811 19.404134108318257 19.216482543592903 68-69 14.298086916355517 44.66107240463451 19.471496208476076 21.569344470533895 70-71 14.634897417144616 41.922321875360865 20.376072982023942 23.06670772547057 72-73 15.049655490973478 37.94699565033296 21.123792293775743 25.879556564917817 74-75 15.193040532737982 37.136725817006045 21.56357057623465 26.106663074021323 76 14.629123522845374 38.432965087185806 21.486585318911427 25.451326071057395 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 1.0 14 1.5 15 0.5 16 0.0 17 0.0 18 1.0 19 6.5 20 18.0 21 25.0 22 19.0 23 20.5 24 35.5 25 43.0 26 66.5 27 117.0 28 171.0 29 198.0 30 225.5 31 307.5 32 443.0 33 524.0 34 618.5 35 841.5 36 1275.0 37 1580.0 38 2716.5 39 4774.0 40 5769.5 41 5421.0 42 4998.0 43 4404.0 44 3723.5 45 3447.5 46 3258.0 47 3126.5 48 2879.5 49 2557.5 50 2351.0 51 2135.5 52 1669.5 53 1341.0 54 1263.0 55 1176.0 56 902.5 57 608.0 58 500.0 59 416.0 60 271.0 61 178.5 62 147.0 63 113.0 64 63.0 65 41.5 66 25.5 67 15.0 68 14.0 69 8.0 70 3.0 71 3.0 72 2.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 51958.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.66938681242542 #Duplication Level Percentage of deduplicated Percentage of total 1 64.90464246841134 26.39632010469995 2 16.350385689271686 13.299203202586703 3 7.377786190904359 9.001501212517802 4 3.8900194027731767 6.328188151968899 5 2.2431498745918317 4.561376496400939 6 1.4481094127111827 3.5336233111359174 7 1.1026454024892338 3.1390738673544014 8 0.6672661019355449 2.1709842565148776 9 0.41644976574700676 1.5243080949998076 >10 1.4907008660262173 8.676238500327187 >50 0.04732383701670532 1.4146041033142154 >100 0.042591453315034784 3.179491127449093 >500 0.004732383701670531 1.12398475691905 >1k 0.014197151105011595 15.651102813811155 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT 3604 6.936371684822356 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT 3388 6.520651295276954 No Hit CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 1140 2.1940798337118443 No Hit CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 584 1.12398475691905 No Hit TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 390 0.7506062589014204 No Hit TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT 308 0.5927864813888141 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 199 0.3830016551830324 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 157 0.3021671349936487 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT 147 0.2829208206628431 No Hit CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 127 0.24442819200123175 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC 111 0.2136340890719427 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGT 107 0.20593556333962046 No Hit CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 106 0.2040109319065399 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGTCTT 100 0.1924631433080565 No Hit CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 100 0.1924631433080565 No Hit CTTATACACATCTCCGAGCCCACGAGACAATTCGTTAACTCGTATGCCGT 99 0.19053851187497595 No Hit TTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC 78 0.15012125178028407 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGTCTT 73 0.14049809461488125 No Hit CTTATTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG 69 0.13279956888255898 No Hit CTTATACACATCTCCGAGCCCACGAGACACTTCGTTATCTCGTATGCCGT 62 0.11932714885099502 No Hit ATACACATCTCCGAGCCCACGAGACAATTCGTTACCTCGTATGCCGTCTT 52 0.1000808345201894 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0038492628661611302 0.0 2 0.0 0.0 0.0 0.0038492628661611302 0.0 3 0.0 0.0 0.0 0.0038492628661611302 0.0 4 0.0 0.0 0.0 0.0076985257323222605 0.0 5 0.0 0.0 0.0 0.0076985257323222605 0.0 6 0.0 0.0 0.0 0.0076985257323222605 0.0 7 0.0 0.0 0.0 0.0076985257323222605 0.0 8 0.0 0.0 0.0 0.0076985257323222605 0.0 9 0.0 0.0 0.0 0.01924631433080565 0.0 10 0.0 0.0 0.0 0.01924631433080565 0.0 11 0.0 0.0 0.0 0.01924631433080565 0.0 12 0.0 0.0 0.0 0.01924631433080565 0.0 13 0.0 0.0 0.0 0.01924631433080565 0.0 14 0.0 0.0 0.0 0.01924631433080565 0.0 15 0.0 0.0 0.0 0.01924631433080565 0.0 16 0.0 0.0 0.0 0.021170945763886215 0.0 17 0.0 0.0 0.0 0.025020208630047346 0.0 18 0.0 0.0 0.0 0.036567997228530734 0.0 19 0.0 0.0 0.0 0.04041726009469187 0.0 20 0.0 0.0 0.0 0.05004041726009469 0.0 21 0.0 0.0 0.0 0.05388968012625582 0.0 22 0.0 0.0 0.0 0.057738942992416956 0.0 23 0.0 0.0 0.0 0.06351283729165864 0.0 24 0.0 0.0 0.0 0.06543746872473921 0.0 25 0.0 0.0 0.0 0.06928673159090035 0.0 26 0.0 0.0 0.0 0.06928673159090035 0.0 27 0.0 0.0 0.0 0.09045767735478656 0.0 28 0.0 0.0 0.0 0.10970399168559221 0.0 29 0.0 0.0 0.0 0.11932714885099503 0.0 30 0.0 0.0 0.0 0.14627198891412294 0.0 31 0.0 0.0 0.0 0.16166904037876748 0.0 32 0.0 0.0 0.0 0.19053851187497595 0.0 33 0.0 0.0 0.0 0.22903114053658724 0.0 34 0.0 0.0 0.0 0.29639324069440703 0.0 35 0.0 0.0 0.0 0.3907001809153547 0.0 36 0.0 0.0 0.0 0.5158012240655915 0.0 37 0.0 0.0 0.0 0.7775510989645483 0.0 38 0.0 0.0 0.0 1.0162053966665383 0.0 39 0.0 0.0 0.0 1.3029754801955427 0.0 40 0.0 0.0 0.0 1.6763539782131722 0.0 41 0.0 0.0 0.0 2.1825320451133607 0.0 42 0.0 0.0 0.0 2.638669694753455 0.0 43 0.0 0.0 0.0 3.1159782901574347 0.0 44 0.0 0.0 0.0 3.5875129912621735 0.0 45 0.0 0.0 0.0 4.05519842950075 0.0 46 0.0 0.0 0.0 4.605643019361792 0.0 47 0.0 0.0 0.0 5.015589514607953 0.0 48 0.0 0.0 0.0 5.5410138958389465 0.0 49 0.0 0.0 0.0 6.02794564840833 0.0 50 0.0 0.0 0.0 6.460987720851457 0.0 51 0.0 0.0 0.0 6.992185996381693 0.0 52 0.0 0.0 0.0 7.517610377612687 0.0 53 0.0 0.0 0.0 7.933330767158089 0.0 54 0.0 0.0 0.0 8.318257053774202 0.0 55 0.0 0.0 0.0 8.778243966280458 0.0 56 0.0 0.0 0.0 9.207436775857424 0.0 57 0.0 0.0 0.0 9.667423688363678 0.0 58 0.0 0.0 0.0 10.081219446476 0.0 59 0.0 0.0 0.0 10.496939836021403 0.0 60 0.0 0.0 0.0 10.976173062858463 0.0 61 0.0 0.0 0.0 11.416913661033911 0.0 62 0.0 0.0 0.0 11.863428153508604 0.0 63 0.0 0.0 0.0 12.300319488817891 0.0 64 0.0 0.0 0.0 12.721813772662536 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTGGA 15 0.002212933 70.00001 2 ATAACAC 30 1.00515535E-7 70.00001 4 TTGGAGA 15 0.002212933 70.00001 4 TCTTTAT 15 0.002212933 70.00001 1 AATTTGT 20 7.8576886E-5 70.0 26 CAATTTG 20 7.8576886E-5 70.0 25 TTTACAC 200 0.0 64.75 2 CTTTACA 205 0.0 63.17073 1 CTTATAA 40 1.0408257E-8 61.25 1 CTACACA 35 2.9206058E-7 60.0 4 TATCCGA 25 2.3697758E-4 55.999996 11 ATCCGAG 25 2.3697758E-4 55.999996 12 ATATCCG 25 2.3697758E-4 55.999996 10 CTATACA 85 0.0 53.52941 1 TTACACA 245 0.0 52.857143 3 TGAGACT 20 0.006914919 52.5 12 ATGGTAC 20 0.006914919 52.5 23 CAATCAA 20 0.006914919 52.5 9 CGAGGCT 20 0.006914919 52.5 51 TTCCATC 20 0.006914919 52.5 61 >>END_MODULE