FastQCFastQC Report
Wed 25 May 2016
SRR1780375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532956
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTGGTC109642.057205472872057No Hit
CTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTGG18840.35350010132168513No Hit
CTTTACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTGG18750.35181140657014837No Hit
TATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTGGT9340.17524898865947658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC13400.037.389844
CGGTGGT14200.036.36659643
CATCGTT401.8301842E-532.9682532
CTATACA3800.032.408111
CGCAAAG703.534842E-828.32496612
TTTACAC5050.028.2763182
TCGGTGG19750.028.04387942
CGAGAAC19200.026.81942722
AGAACGG19200.026.70732524
CTGTGTA18800.026.53827531
TCGTTGT508.281021E-526.42420634
GAACGGC19400.026.40221625
CTTTACA5500.026.3894631
CTCGGTG21200.025.91843441
ACGGCTG19950.025.66951627
TCTCGGT21450.025.56187640
AACGGCT20050.025.54148926
CGGCTGT20050.025.43186828
GAGAACG20450.025.07249323
ATCTCGG23200.024.58286539
Produced by FastQC (version 0.11.5)