FastQCFastQC Report
Wed 25 May 2016
SRR1780375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532956
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT437648.211559678472518No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT363216.815009118951658RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC112262.1063652534167923No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC61641.156568272052477No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG56071.0520568302073718No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT31960.5996742695457036RNA PCR Primer, Index 43 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC25280.47433559243164536No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC19780.3711375798377352No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG15500.29083076276465597No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC12720.23866885821718867No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG11560.21690345919738213No Hit
CTTAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC9210.1728097629072569No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC8310.15592281539188976No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG7320.13734717312498593No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG6730.12627684086491192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA1050.044.0374681
CGTTGAG207.85606E-443.9961530
CTGCTTG15650.043.433924
TTGAAAA15950.043.0307228
CTTGAAA15950.042.7548827
TCTTCTG15750.042.7391220
CGTCTTC16100.042.35654418
CTTCTGC16600.041.21326421
GCTTGAA16400.041.17932526
GTCTTCT16700.040.96647619
TGAAAAA17100.040.3941329
TGCTTGA16700.040.30785425
TCTGCTT17250.040.17040323
TTCTGCT17150.040.01982522
TTTACAC14550.036.594732
GAAAAAA18950.036.45063830
CTTTACA14650.036.2219431
CTATACA8100.036.1542171
TCTTTAT2450.035.948951
AGACCCG250.00234937835.20683310
Produced by FastQC (version 0.11.5)