FastQCFastQC Report
Wed 25 May 2016
SRR1780374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59466
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATG150.00221609669.9873950
GAGCCGA150.00221609669.9873911
ATATAGG200.00658523253.1611981
TGAGCCG200.006924750352.4905410
AGTGTAA200.006924750352.4905435
TCGGTGG305.8394676E-446.65825745
TCTCGGT350.001247294539.9927943
CGGTGGT400.00240321334.9936946
ATCTCGG500.007162659427.99495542
AATGGCT500.007162659427.99495522
AAGTGTA657.878717E-426.91822234
ATGTTGT800.002640561221.8710586
ATGCACG950.007139880718.41773470
GCCTGTC1250.001738302416.79697259
CGACGAT2954.0563464E-1016.60717670
ACGATGC2052.1119107E-413.656075570
CCGACGA4002.9629518E-812.24779369
GACGATG2459.4905775E-411.42651269
TGCCGAC4803.1164745E-810.93552970
GCCGACG4207.4220407E-710.83138168