##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780374_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59466 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.994803753405307 34.0 23.0 34.0 12.0 34.0 2 28.692866511956414 34.0 23.0 34.0 11.0 34.0 3 28.947650758416575 34.0 23.0 34.0 11.0 34.0 4 29.107607708606597 34.0 27.0 34.0 11.0 34.0 5 28.954141862576936 34.0 27.0 34.0 11.0 34.0 6 31.43243197793697 37.0 27.0 38.0 11.0 38.0 7 31.274291191605286 37.0 27.0 38.0 11.0 38.0 8 30.59322974472808 36.0 26.0 38.0 11.0 38.0 9 31.09333064272021 37.0 26.0 38.0 12.0 38.0 10-11 31.10813742306528 37.0 26.5 38.0 11.0 38.0 12-13 31.37166616217671 37.0 27.0 38.0 11.0 38.0 14-15 31.04498368815794 37.0 26.0 38.0 11.0 38.0 16-17 30.820014798372178 37.0 26.0 38.0 11.0 38.0 18-19 30.860020852251708 37.0 26.0 38.0 11.0 38.0 20-21 30.908939562102717 37.0 26.0 38.0 11.0 38.0 22-23 30.815028755927756 37.0 26.0 38.0 11.0 38.0 24-25 31.131444859247303 37.0 26.0 38.0 11.0 38.0 26-27 31.091901254498367 37.0 26.0 38.0 11.0 38.0 28-29 31.09747586856355 37.0 26.0 38.0 11.0 38.0 30-31 30.98794268994047 37.0 26.0 38.0 11.0 38.0 32-33 30.815592103050484 37.0 26.0 38.0 11.0 38.0 34-35 30.71952880637675 37.0 26.0 38.0 11.0 38.0 36-37 30.73227558604917 37.0 26.0 38.0 11.0 38.0 38-39 30.99636767228332 37.0 26.0 38.0 11.0 38.0 40-41 30.731417953116065 37.0 26.0 38.0 11.0 38.0 42-43 30.990128813103286 37.0 26.0 38.0 11.0 38.0 44-45 30.22222782766623 36.0 25.0 38.0 11.0 38.0 46-47 30.77746107019137 37.0 26.0 38.0 11.0 38.0 48-49 30.634623145999395 37.0 26.0 38.0 11.0 38.0 50-51 30.622338815457574 37.0 25.5 38.0 11.0 38.0 52-53 30.65502976490768 37.0 26.0 38.0 11.0 38.0 54-55 30.446557696835164 36.5 25.0 38.0 11.0 38.0 56-57 30.392922005852085 36.5 24.5 38.0 11.0 38.0 58-59 30.30845357010729 36.5 24.5 38.0 11.0 38.0 60-61 30.378972858440115 36.5 24.5 38.0 11.0 38.0 62-63 30.394342985907915 36.5 25.0 38.0 11.0 38.0 64-65 30.439957286516666 36.5 25.0 38.0 11.0 38.0 66-67 30.440503817307366 36.5 24.5 38.0 11.0 38.0 68-69 30.03142972454848 36.0 23.5 38.0 11.0 38.0 70-71 29.899497191672552 36.0 24.0 38.0 11.0 38.0 72-73 29.992886691554837 36.0 24.0 38.0 11.0 38.0 74-75 29.64816870144284 35.5 23.5 38.0 11.0 38.0 76 28.915413849931053 34.0 22.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.5731284832324022 1101 2 0.08397121602127555 1101 3 0.298871951962667 1101 4 -1.3603279780935935 1101 5 -0.9653792125586946 1101 6 -1.0260379579052419 1101 7 0.18779144799363223 1101 8 -0.028164473668663703 1101 9 0.14506666578691352 1101 10-11 -0.33482798998593566 1101 12-13 0.033391712598003664 1101 14-15 0.08806895053081831 1101 16-17 -0.3082245198901923 1101 18-19 0.38180977465356847 1101 20-21 -0.1465429680807695 1101 22-23 0.36055283468947863 1101 24-25 0.1975315628744383 1101 26-27 -0.6774074071320335 1101 28-29 0.2270976519753063 1101 30-31 -0.2772459488907977 1101 32-33 -0.02863727507780922 1101 34-35 -1.3682484054131088 1101 36-37 0.13283157389269462 1101 38-39 -0.7158349059452753 1101 40-41 0.05433869437214511 1101 42-43 -0.20489115298795468 1101 44-45 0.49534518465175026 1101 46-47 1.1526296185709377 1101 48-49 -0.3871842003916548 1101 50-51 -0.5562964468851206 1101 52-53 0.06201749800138856 1101 54-55 -0.6562463194948478 1101 56-57 -0.4410546881171662 1101 58-59 0.029732741231267568 1101 60-61 0.6663935810369139 1101 62-63 -1.0491550466159865 1101 64-65 -0.5639444800963531 1101 66-67 -0.7162437437322495 1101 68-69 0.987766282789103 1101 70-71 0.5117494178676836 1101 72-73 -0.2893525741904668 1101 74-75 -1.4601398829758132 1101 76 -1.0987593602468415 1102 1 0.14313132496288716 1102 2 -0.4589668833684364 1102 3 0.09220237916406049 1102 4 -0.1518275421738018 1102 5 -0.08394939564500703 1102 6 0.7937963925742721 1102 7 0.45736424659869 1102 8 1.1283743231927161 1102 9 1.0265400746796765 1102 10-11 -0.4331714947810532 1102 12-13 -3.0460328732781967 1102 14-15 -0.6971315725729319 1102 16-17 -2.685491302802138 1102 18-19 -0.35528699953997744 1102 20-21 -0.08438080591860952 1102 22-23 -0.8653190048920365 1102 24-25 -0.14841438307150412 1102 26-27 -3.245149342615907 1102 28-29 0.26916391178350096 1102 30-31 0.578486396357679 1102 32-33 -4.824060117274847 1102 34-35 -0.5905239067208647 1102 36-37 0.22053863579330368 1102 38-39 0.43804478019247384 1102 40-41 0.3921111442413725 1102 42-43 0.2702178269597333 1102 44-45 -5.283360133569698 1102 46-47 -0.5393930492581838 1102 48-49 -0.5592853337831087 1102 50-51 -5.858083718027231 1102 52-53 -1.7231394331232828 1102 54-55 0.3510989289794324 1102 56-57 -1.2256667195033906 1102 58-59 0.05874320330624627 1102 60-61 -0.43881566046265164 1102 62-63 -4.61308268393072 1102 64-65 -1.0289401208984437 1102 66-67 -0.3488505440809817 1102 68-69 -4.994818721570095 1102 70-71 -0.8644711575464434 1102 72-73 -0.6918591129873306 1102 74-75 -6.601247119244338 1102 76 -1.8216451492616628 1103 1 -0.7356462641712369 1103 2 -1.0501760891786631 1103 3 -0.16710718506910283 1103 4 -0.274860932100232 1103 5 0.4133152713989503 1103 6 -1.054685026025993 1103 7 0.7926594739142914 1103 8 1.3620283507346898 1103 9 0.6391791993731282 1103 10-11 0.4750345040870805 1103 12-13 -1.4892888627141545 1103 14-15 0.035654694169192425 1103 16-17 -0.529992340342222 1103 18-19 -0.5319329154049086 1103 20-21 -0.11595975328702934 1103 22-23 -0.18684324560133803 1103 24-25 0.5381353245308347 1103 26-27 -1.1481676410501613 1103 28-29 0.16399507571483696 1103 30-31 0.5391164661558285 1103 32-33 -3.57933270735219 1103 34-35 -0.9842986662002104 1103 36-37 0.1668129236635174 1103 38-39 0.882394902810848 1103 40-41 0.4617395162466522 1103 42-43 0.2861393512004433 1103 44-45 -3.312996051321065 1103 46-47 -0.2901947847797821 1103 48-49 0.6616541116045553 1103 50-51 0.3139026996298142 1103 52-53 0.39987509243793085 1103 54-55 0.47882954584417803 1103 56-57 0.20794957930815272 1103 58-59 0.9866435994579135 1103 60-61 1.2511296328782286 1103 62-63 0.6464155239545875 1103 64-65 0.6138253950419461 1103 66-67 0.08848203467773885 1103 68-69 -2.382255054337495 1103 70-71 0.18282180604533593 1103 72-73 1.436682671581618 1103 74-75 -0.7057934312609397 1103 76 0.7190452865051711 1104 1 -1.3693923942021975 1104 2 0.04748472953478711 1104 3 0.23186082318683177 1104 4 0.035458985340429905 1104 5 -0.5606097372009842 1104 6 -0.1692017098925227 1104 7 0.8636261061812291 1104 8 2.3145223307828466 1104 9 0.27265013160567264 1104 10-11 0.8144565887104029 1104 12-13 -0.18075773096320802 1104 14-15 0.29812459440999106 1104 16-17 0.17456562319406643 1104 18-19 0.04651565700650906 1104 20-21 0.3038544881990397 1104 22-23 0.7498612607626107 1104 24-25 1.6339385581049655 1104 26-27 0.5872984752209085 1104 28-29 0.004363152770217482 1104 30-31 -0.2830885562039889 1104 32-33 0.21094937039118733 1104 34-35 0.57380246899071 1104 36-37 -0.009537265534969919 1104 38-39 0.0322573039116385 1104 40-41 0.6991320171066455 1104 42-43 -0.028738529776507704 1104 44-45 -1.445314592772732 1104 46-47 0.8452369317664825 1104 48-49 0.7755344005622433 1104 50-51 1.1553887677412682 1104 52-53 0.8331224264592585 1104 54-55 0.6437934261331364 1104 56-57 0.6887545328685256 1104 58-59 0.6889139812948564 1104 60-61 0.18423936800034824 1104 62-63 0.838245589314063 1104 64-65 0.47921927189092983 1104 66-67 0.001244332579357632 1104 68-69 -3.021573939786414 1104 70-71 -0.8608658444534605 1104 72-73 0.2341370919462591 1104 74-75 -0.509027005392344 1104 76 0.3827191771140477 1105 1 -1.147155807259729 1105 2 -0.21295483090477418 1105 3 -0.8872729841822675 1105 4 0.6081157403501258 1105 5 0.5465007993213185 1105 6 -0.735200417067702 1105 7 0.8217385819407639 1105 8 0.11387591837172906 1105 9 -0.0069232362029900685 1105 10-11 0.6661372609793119 1105 12-13 -0.36265818345189516 1105 14-15 0.033903521365388656 1105 16-17 0.20889012222444947 1105 18-19 -0.25005835721456293 1105 20-21 0.334300512762713 1105 22-23 0.9003137494503974 1105 24-25 -0.2594033940605165 1105 26-27 0.4050101752855504 1105 28-29 -0.4128637379860862 1105 30-31 0.32495927831443083 1105 32-33 0.9490877026471694 1105 34-35 0.7896072524425506 1105 36-37 0.4265648676259879 1105 38-39 -0.531026471136844 1105 40-41 -0.09828233324887847 1105 42-43 0.06263186453506364 1105 44-45 0.8130241273306922 1105 46-47 0.31003977598109245 1105 48-49 0.22782916462170988 1105 50-51 0.06896342837475089 1105 52-53 -0.7521716911878009 1105 54-55 -0.2463487845973127 1105 56-57 0.0370415399790609 1105 58-59 -0.1410795545810295 1105 60-61 -0.32258028293694707 1105 62-63 0.7440956216346812 1105 64-65 0.5078553417034684 1105 66-67 0.7128474353589311 1105 68-69 0.5931642631870808 1105 70-71 0.4174146985329621 1105 72-73 0.12492571508781936 1105 74-75 -0.16158033441626074 1105 76 -0.22639523788271987 1106 1 0.9695502714716682 1106 2 1.2272315649778243 1106 3 0.9133947025613658 1106 4 0.7492341156457165 1106 5 1.0818325561720634 1106 6 0.9447968009042 1106 7 1.8422560391670828 1106 8 2.3874188311551485 1106 9 3.0820321122460292 1106 10-11 1.3523940421728007 1106 12-13 1.6806719041819846 1106 14-15 0.9917210203221245 1106 16-17 1.486293098994512 1106 18-19 1.087505977185213 1106 20-21 0.9206824852206346 1106 22-23 0.9214919383501012 1106 24-25 1.3781678954095042 1106 26-27 1.7055039852730296 1106 28-29 0.32673843199583175 1106 30-31 1.0861221660595994 1106 32-33 1.7024609117165852 1106 34-35 1.4549640475461807 1106 36-37 0.4912817962669678 1106 38-39 1.4975588675750835 1106 40-41 0.5860270282756694 1106 42-43 1.8944930413329466 1106 44-45 2.3203965017916772 1106 46-47 1.8826159340002881 1106 48-49 1.0868151153798138 1106 50-51 1.2865875763786434 1106 52-53 1.0326384353944782 1106 54-55 1.2419319220378249 1106 56-57 1.3223732968298059 1106 58-59 0.917134383379743 1106 60-61 2.1572847887002737 1106 62-63 1.965684159678915 1106 64-65 1.6456005079296467 1106 66-67 1.7385116445674456 1106 68-69 2.3430947124846178 1106 70-71 1.774279352865971 1106 72-73 1.6384185513654685 1106 74-75 -0.3839339301875775 1106 76 0.014737047553364135 1107 1 -0.25285821296213484 1107 2 -0.5768395947895364 1107 3 -0.3376145345238193 1107 4 -0.7805982483638658 1107 5 -0.33835218553166513 1107 6 -0.8463082281755128 1107 7 -1.077073416871233 1107 8 -0.6214077169119072 1107 9 -1.4670954963752507 1107 10-11 -1.2078009629589097 1107 12-13 -0.9612028819965914 1107 14-15 -1.330174292712428 1107 16-17 -0.39133262799829893 1107 18-19 -0.08440644156264909 1107 20-21 0.0967002751624726 1107 22-23 -1.794176895040252 1107 24-25 -0.7484143830715055 1107 26-27 -1.207137136861764 1107 28-29 -2.3607401858625323 1107 30-31 -1.0292729759178236 1107 32-33 0.299741103300569 1107 34-35 -0.3263565135212154 1107 36-37 -0.7104116693505489 1107 38-39 -0.613132203242575 1107 40-41 -0.5382288731954183 1107 42-43 -0.8224587205555203 1107 44-45 -0.15397913967257182 1107 46-47 -0.8000730841317676 1107 48-49 -0.6412382544457103 1107 50-51 -0.2387288793175557 1107 52-53 -0.1467662857823946 1107 54-55 -1.1244895627380913 1107 56-57 -0.5559195529820329 1107 58-59 -0.9831050966065753 1107 60-61 -0.682930743287411 1107 62-63 -1.0322631547240917 1107 64-65 -0.5058363719882486 1107 66-67 0.36213463464613227 1107 68-69 -0.3034499334271139 1107 70-71 -0.2200073388890722 1107 72-73 -0.4521904120283047 1107 74-75 -0.34257231540824407 1107 76 -0.6082188197063019 1108 1 -1.0404708255747472 1108 2 0.008199015249072517 1108 3 0.24047426856498433 1108 4 -0.02329451606013322 1108 5 0.1591381619586798 1108 6 -0.17018210204938455 1108 7 0.6628557980579828 1108 8 0.47376602826183856 1108 9 0.06221595793620338 1108 10-11 0.32982773716978997 1108 12-13 0.3818473110535976 1108 14-15 0.8324383199001844 1108 16-17 -0.31079852246419293 1108 18-19 0.459190727034521 1108 20-21 1.2566906226528225 1108 22-23 0.19243829157493764 1108 24-25 0.27539514073801996 1108 26-27 -0.2339550261796539 1108 28-29 0.9234457858234393 1108 30-31 0.9300416958968505 1108 32-33 0.2750950286544942 1108 34-35 0.45100835134364914 1108 36-37 -0.04710729354617271 1108 38-39 1.19745338234301 1108 40-41 0.8728715129049647 1108 42-43 0.7885934029966002 1108 44-45 0.6127036145101812 1108 46-47 0.781651497592815 1108 48-49 -0.02239977310722807 1108 50-51 0.2940092159205392 1108 52-53 0.5573521183360093 1108 54-55 0.8933032300547055 1108 56-57 1.1526550930926156 1108 58-59 -0.0630117890132631 1108 60-61 0.9706889478322793 1108 62-63 -0.10160034906128956 1108 64-65 0.1873425211906472 1108 66-67 -0.024560989549492263 1108 68-69 1.1620745195973399 1108 70-71 1.541270009888276 1108 72-73 0.7950264476885565 1108 74-75 1.1891522663163379 1108 76 -0.08765897414645707 1109 1 0.3460234272988174 1109 2 -0.5260784888560153 1109 3 -0.279024910416954 1109 4 0.4247760914932357 1109 5 0.8886127103987462 1109 6 0.6832661738126831 1109 7 -0.007300195373872498 1109 8 1.1851781793291636 1109 9 1.4721092255880954 1109 10-11 1.1296019572026275 1109 12-13 0.7040969005445703 1109 14-15 0.706001538290991 1109 16-17 0.24400443320083198 1109 18-19 0.6059477056880525 1109 20-21 0.7828605733917371 1109 22-23 0.6481033162054786 1109 24-25 0.8636545824457329 1109 26-27 1.0955810986150887 1109 28-29 0.453720826874342 1109 30-31 0.7766139454863357 1109 32-33 1.4721393636298643 1109 34-35 0.48046237433215566 1109 36-37 0.3474945126935616 1109 38-39 0.9661315432625521 1109 40-41 0.8981178183236871 1109 42-43 1.335494059811051 1109 44-45 1.1869031218993413 1109 46-47 0.5944593793662172 1109 48-49 -0.10604098821396235 1109 50-51 1.631960775854857 1109 52-53 1.2564489984673735 1109 54-55 0.8315216208593021 1109 56-57 1.3705327614013179 1109 58-59 1.488609721659973 1109 60-61 1.5736240874053493 1109 62-63 0.7364538381308279 1109 64-65 0.8129582847374444 1109 66-67 0.5568224258856489 1109 68-69 1.6410359317484087 1109 70-71 0.4344350345188168 1109 72-73 0.8234541980990642 1109 74-75 1.8726498032621528 1109 76 -0.28367703654382836 1110 1 0.3125730866009029 1110 2 0.12592784330724172 1110 3 -0.27310571432637687 1110 4 0.8689566958018595 1110 5 0.5434409848842598 1110 6 0.838300931882749 1110 7 0.7582721082946549 1110 8 2.24643497608049 1110 9 -1.2844122142907715 1110 10-11 -0.03809712738680915 1110 12-13 0.20113730249927997 1110 14-15 0.4127776412574704 1110 16-17 0.8209023614822009 1110 18-19 1.047977439324228 1110 20-21 0.4617269785125302 1110 22-23 0.3338697144434839 1110 24-25 0.18272334147939162 1110 26-27 -0.8271080059344307 1110 28-29 0.09184113797627447 1110 30-31 -0.6860297326745801 1110 32-33 0.8391811421418041 1110 34-35 1.2941969740981456 1110 36-37 0.4337702371068062 1110 38-39 0.9896190510056861 1110 40-41 0.7876491005867443 1110 42-43 0.6147803148871063 1110 44-45 1.7380422230074686 1110 46-47 0.7732397496601102 1110 48-49 0.8618852283184921 1110 50-51 0.10558249792795493 1110 52-53 -0.07851899657702077 1110 54-55 0.18646690270939104 1110 56-57 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2116 50-51 -0.32597720146520714 2116 52-53 0.6102444161947496 2116 54-55 0.5960437222247243 2116 56-57 0.13218129954835334 2116 58-59 -0.5335495007422502 2116 60-61 -1.1526931231169044 2116 62-63 0.7769151510985068 2116 64-65 0.711631431385598 2116 66-67 -0.02406162873134221 2116 68-69 0.26839842499842703 2116 70-71 -1.9171232467432375 2116 72-73 0.3895654377812363 2116 74-75 -0.7836026074452498 2116 76 -1.0652476606277617 2117 1 0.41215474520150863 2117 2 0.24474500350738992 2117 3 0.8918930552577429 2117 4 0.37486269202858225 2117 5 -0.25230997894151486 2117 6 -1.244383015291394 2117 7 -2.5661885594110316 2117 8 -2.767427709507242 2117 9 -0.1330710609809529 2117 10-11 -0.7438431695490628 2117 12-13 0.21110367113187678 2117 14-15 -0.31710505909524755 2117 16-17 0.08214015985804934 2117 18-19 -0.21750529383958295 2117 20-21 -0.17958628537066446 2117 22-23 0.36791122047252145 2117 24-25 0.26870890459972685 2117 26-27 0.7814967024570088 2117 28-29 0.2055315653407277 2117 30-31 0.1393127330788424 2117 32-33 1.0281935283283374 2117 34-35 0.7035768444943322 2117 36-37 -0.1824220358814621 2117 38-39 0.2585264417037436 2117 40-41 -1.0627938491302622 2117 42-43 -0.5373606087450824 2117 44-45 0.6160875023118777 2117 46-47 -0.8478331729877446 2117 48-49 0.03488170112630229 2117 50-51 1.3331889289016274 2117 52-53 1.3985132403190441 2117 54-55 -0.5410575005389049 2117 56-57 -0.46039937527007524 2117 58-59 -0.026033641589911838 2117 60-61 -0.017936296786114525 2117 62-63 -0.7215449022250198 2117 64-65 0.8393320841391123 2117 66-67 -0.7557661428436901 2117 68-69 -0.38982534993951745 2117 70-71 0.11314704837490197 2117 72-73 0.8317599767166044 2117 74-75 -0.3615652809765528 2117 76 0.2780455268320168 2118 1 0.5867221568813932 2118 2 0.656695255850579 2118 3 0.08069983247475676 2118 4 0.7799636293032783 2118 5 0.3095141018083041 2118 6 0.9657828102313175 2118 7 0.9783319885341726 2118 8 -2.40405352061034 2118 9 0.21885756194622985 2118 10-11 0.029859065490590808 2118 12-13 1.1064713712039733 2118 14-15 -0.52172834676648 2118 16-17 -0.15353035203812837 2118 18-19 -0.5293744358727466 2118 20-21 -1.8067492269712382 2118 22-23 -0.9002935379989978 2118 24-25 -0.5806512251767657 2118 26-27 0.20746101392059657 2118 28-29 -0.5110780237003709 2118 30-31 0.0541018462727898 2118 32-33 0.5299509183788018 2118 34-35 0.931271509238389 2118 36-37 0.021345087406206886 2118 38-39 -0.42316065274470915 2118 40-41 -0.924590893110075 2118 42-43 -0.4510682511387394 2118 44-45 0.18773494283098202 2118 46-47 -0.7642444923821223 2118 48-49 0.06516288353437716 2118 50-51 0.03100796278770801 2118 52-53 -0.6765137964509584 2118 54-55 0.06983831777399985 2118 56-57 0.8329495793081527 2118 58-59 1.2026523713877388 2118 60-61 -0.9893674431451629 2118 62-63 0.6175412549487334 2118 64-65 0.04291896229340608 2118 66-67 -1.24558229280764 2118 68-69 0.04537652460987118 2118 70-71 -0.4626460302119746 2118 72-73 -1.002279316722472 2118 74-75 -0.3567437236585995 2118 76 0.05237861983850678 2119 1 -1.354864764968685 2119 2 0.07475745680751444 2119 3 -0.1356079825172678 2119 4 -0.7565196242852394 2119 5 -0.7239787211582005 2119 6 -0.9277578596251423 2119 7 -1.0671225569203742 2119 8 -0.03922098472517277 2119 9 -0.9902732195529715 2119 10-11 -1.041113821271768 2119 12-13 -0.7880661088598195 2119 14-15 -1.2433192558573865 2119 16-17 -1.7290885657542354 2119 18-19 -0.9363720435282517 2119 20-21 -1.8555929271307292 2119 22-23 -0.8963062971696516 2119 24-25 -0.8128083224654432 2119 26-27 -0.9349831647077913 2119 28-29 -0.8766083267306755 2119 30-31 -0.8716357932806424 2119 32-33 -0.8401647116052473 2119 34-35 -0.4410371032018041 2119 36-37 -0.6680488519877308 2119 38-39 0.14442307931270904 2119 40-41 -0.03513714510369326 2119 42-43 -0.01795421605102021 2119 44-45 -2.167328853022287 2119 46-47 -1.1393441734028542 2119 48-49 -1.0957222839297387 2119 50-51 -0.5985773641660401 2119 52-53 -1.9226262366423477 2119 54-55 -1.1748785881271147 2119 56-57 -0.10465010171258271 2119 58-59 0.1006786871772114 2119 60-61 -0.5593435197001888 2119 62-63 -0.4646955871565268 2119 64-65 0.061260270108395076 2119 66-67 -0.5394419419304484 2119 68-69 -1.6753172552944342 2119 70-71 -1.40542912431086 2119 72-73 -2.3957203055582 2119 74-75 -0.5916918895278194 2119 76 -0.27975854124602506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 9.0 12 86.0 13 401.0 14 881.0 15 1282.0 16 1531.0 17 1837.0 18 2135.0 19 2076.0 20 1811.0 21 1486.0 22 1138.0 23 862.0 24 757.0 25 687.0 26 767.0 27 757.0 28 897.0 29 1020.0 30 1355.0 31 1612.0 32 2140.0 33 2759.0 34 3768.0 35 5535.0 36 9472.0 37 12405.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.180405302453146 28.101985880440854 10.09362249648704 29.623986320618954 2 16.653213601049337 24.698146840211212 34.29859079137659 24.350048767362864 3 15.571923452056637 30.07937308714223 27.847845827868024 26.500857632933105 4 11.949685534591195 22.537248175427976 35.81037904012377 29.702687249857064 5 13.646453435576634 34.96283590623213 32.79857397504456 18.592136683146673 6 24.45094675949282 37.04133454410924 17.882487471832643 20.625231224565297 7 21.7805132344533 35.82215047253893 19.71546766219352 22.681868630814247 8 19.179026670702587 38.21511451922107 19.054585813742307 23.55127299633404 9 19.65829213331988 23.941411899236538 19.4548145158578 36.945481451585785 10-11 20.177075976188075 28.723976726196483 25.92994316079777 25.16900413681768 12-13 20.0770188006592 26.3184004304981 25.909763562371772 27.694817206470923 14-15 19.6524064171123 27.77637641677597 22.353109339790805 30.21810782632092 16-17 20.118218814112264 29.237715669458176 24.095281337234724 26.548784179194833 18-19 18.760300003363266 28.433054182221774 24.956277536743684 27.850368277671272 20-21 18.890626576531126 28.855984932566507 24.673763158779806 27.57962533212256 22-23 20.341876030000336 28.78199307167121 24.355934483570444 26.52019641475801 24-25 19.23115729996973 30.110483301382303 23.964954764066864 26.693404634581103 26-27 18.86624289509972 29.91625466653214 25.127804123364612 26.089698315003528 28-29 19.59270843843541 28.96360945750513 26.43779638785188 25.005885716207583 30-31 19.554871691386673 28.979584972925704 25.983755423267073 25.481787912420543 32-33 18.46096929337773 30.035650623885918 25.517102209666024 25.986277873070325 34-35 19.4548145158578 28.959405374499713 26.176302424915075 25.409477684727406 36-37 19.357279790132175 28.46332357986076 26.442000470857295 25.737396159149768 38-39 18.991524568661085 27.657821276023277 27.530017152658665 25.820637002656984 40-41 18.471059092590725 27.54935593448357 26.93808226549625 27.041502707429455 42-43 17.441899572865164 26.494971916725525 28.326270473884236 27.736858036525074 44-45 17.492348568930144 26.968351663135238 28.472572562472674 27.066727205461945 46-47 17.79420172871893 27.43584569333737 28.01348669828137 26.75646587966233 48-49 17.320821982309216 27.998351999461878 27.60569064675613 27.075135371472776 50-51 17.266168903238828 27.631755961389704 27.194531328826553 27.907543806544915 52-53 16.215988968486194 28.15474388726331 27.07597618807386 28.553290956176642 54-55 16.305956344802073 28.198466350519624 26.778327111290483 28.717250193387816 56-57 16.915548380587225 28.208556149732622 26.392392291393403 28.48350317828675 58-59 16.37322167288871 27.744425385934818 26.54205764638617 29.3402952947903 60-61 16.437123734571017 27.270204822924022 26.61857195708472 29.674099485420243 62-63 16.685164631890494 28.032825480106276 26.003094205091987 29.278915682911244 64-65 17.35529546295362 28.34140517270373 24.76457135169677 29.538728012645883 66-67 17.15686274509804 29.291527931927487 23.757273063599367 29.794336259375104 68-69 17.143409679480712 28.578515454209132 23.9927017119026 30.285373154407562 70-71 17.718528234621463 28.665119564120673 24.06417112299465 29.552181078263207 72-73 17.858944607002318 28.879527797396833 23.14936266101638 30.112164934584467 74-75 18.10782632092288 28.796286953889616 23.131705512393637 29.964181212793868 76 17.803450711330846 29.15951972555746 23.401607641341272 29.635421921770423 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 2.0 10 2.5 11 3.5 12 5.0 13 4.5 14 7.0 15 8.5 16 6.5 17 6.0 18 14.5 19 31.0 20 43.0 21 47.0 22 49.5 23 65.0 24 107.5 25 137.0 26 174.0 27 256.0 28 330.5 29 360.0 30 423.0 31 581.0 32 842.0 33 1008.0 34 1128.0 35 1387.0 36 1719.5 37 1913.0 38 2101.0 39 2538.5 40 3010.0 41 3442.0 42 3652.0 43 3774.5 44 4042.5 45 4199.0 46 4210.0 47 4163.5 48 3989.5 49 3663.5 50 3465.0 51 3276.0 52 2903.5 53 2471.5 54 2223.0 55 2095.0 56 1702.0 57 1284.0 58 1131.0 59 993.0 60 720.5 61 535.5 62 485.0 63 407.5 64 289.0 65 206.5 66 145.0 67 125.0 68 91.0 69 43.5 70 33.0 71 36.0 72 30.0 73 24.5 74 20.0 75 15.0 76 13.0 77 10.0 78 11.5 79 14.0 80 10.0 81 5.0 82 4.5 83 5.0 84 4.0 85 2.0 86 1.0 87 1.0 88 2.5 89 2.0 90 0.0 91 0.5 92 1.0 93 1.5 94 3.0 95 3.0 96 2.0 97 1.0 98 1.5 99 4.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6709716476642115 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59466.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.68748528570949 #Duplication Level Percentage of deduplicated Percentage of total 1 82.62169378580067 60.881848451215824 2 9.340696957940619 13.765849392930413 3 3.9868550172299684 8.81343961255171 4 1.7914603254296082 5.280328254801063 5 0.8740500696045095 3.220327582147782 6 0.47924416349072324 2.118857834729089 7 0.31721399392957395 1.6362291057074632 8 0.20767247084598006 1.224228971176807 9 0.13692690385449233 0.9080819291696095 >10 0.24418631187384468 2.1508088655702418 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0016816332021659436 0.0 2 0.0 0.0 0.0 0.0016816332021659436 0.0 3 0.0 0.0 0.0 0.003363266404331887 0.0 4 0.0 0.0 0.0 0.003363266404331887 0.0 5 0.0 0.0 0.0 0.003363266404331887 0.0 6 0.0 0.0 0.0 0.003363266404331887 0.0 7 0.0 0.0 0.0 0.003363266404331887 0.0 8 0.0 0.0 0.0 0.003363266404331887 0.0 9 0.0 0.0 0.0 0.006726532808663774 0.0 10 0.0 0.0 0.0 0.011771432415161605 0.0 11 0.0 0.0 0.0 0.015134698819493492 0.0 12 0.0 0.0 0.0 0.016816332021659434 0.0 13 0.0 0.0 0.0 0.016816332021659434 0.0 14 0.0 0.0 0.0 0.016816332021659434 0.0 15 0.0 0.0 0.0 0.020179598425991322 0.0 16 0.0 0.0 0.0 0.02354286483032321 0.0 17 0.0 0.0 0.0 0.02354286483032321 0.0 18 0.0 0.0 0.0 0.025224498032489155 0.0 19 0.0 0.0 0.0 0.02858776443682104 0.0 20 0.0 0.0 0.0 0.0386775636498167 0.0 21 0.0 0.0 0.0 0.040359196851982644 0.0 22 0.0 0.0 0.0 0.040359196851982644 0.0 23 0.0 0.0 0.0 0.040359196851982644 0.0 24 0.0 0.0 0.0 0.04204083005414859 0.0 25 0.0 0.0 0.0 0.045404096458480474 0.0 26 0.0 0.0 0.0 0.058857162075808024 0.0 27 0.0 0.0 0.0 0.08744492651262907 0.0 28 0.0 0.0 0.0 0.12107759055594794 0.0 29 0.0 0.0 0.0 0.13621228937544144 0.0 30 0.0 0.0 0.0 0.1547102545992668 0.0 31 0.0 0.0 0.0 0.19338781824908352 0.0 32 0.0 0.0 0.0 0.25560824672922344 0.0 33 0.0 0.0 0.0 0.32960010762452496 0.0 34 0.0 0.0 0.0 0.4153634009349881 0.0 35 0.0 0.0 0.0 0.49944506104328523 0.0 36 0.0 0.0 0.0 0.684424713281539 0.0 37 0.0 0.0 0.0 0.9787105236605792 0.0 38 0.0 0.0 0.0 1.3638045269565802 0.0 39 0.0 0.0 0.0 1.7152658662092624 0.0 40 0.0 0.0 0.0 2.2483435912958667 0.0 41 0.0 0.0 0.0 2.8150539804257897 0.0 42 0.0 0.0 0.0 3.517976658931154 0.0 43 0.0 0.0 0.0 4.3117075303534795 0.0 44 0.0 0.0 0.0 4.99949551003935 0.0 45 0.0 0.0 0.0 5.670467157703562 0.0 46 0.0 0.0 0.0 6.4339286314869 0.0 47 0.0 0.0 0.0 7.148622742407426 0.0 48 0.0 0.0 0.0 7.833047455688965 0.0 49 0.0 0.0 0.0 8.35771701476474 0.0 50 0.0 0.0 0.0 8.894158006255676 0.0 51 0.0 0.0 0.0 9.504590858641913 0.0 52 0.0 0.0 0.0 10.286550297649077 0.0 53 0.0 0.0 0.0 11.03151380620859 0.0 54 0.0 0.0 0.0 11.771432415161605 0.0 55 0.0 0.0 0.0 12.470991827262637 0.0 56 0.0 0.0 0.0 13.103285911277032 0.0 57 0.0 0.0 0.0 13.641408535970134 0.0 58 0.0 0.0 0.0 14.258567921165035 0.0 59 0.0 0.0 0.0 14.653751723674032 0.0 60 0.0 0.0 0.0 15.17673964954764 0.0 61 0.0 0.0 0.0 15.647596946154104 0.0 62 0.0 0.0 0.0 16.07977667911075 0.0 63 0.0 0.0 0.0 16.607809504590858 0.0 64 0.0 0.0 0.0 17.256919920626913 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTATG 15 0.002216096 69.98739 50 GAGCCGA 15 0.002216096 69.98739 11 ATATAGG 20 0.006585232 53.161198 1 TGAGCCG 20 0.0069247503 52.49054 10 AGTGTAA 20 0.0069247503 52.49054 35 TCGGTGG 30 5.8394676E-4 46.658257 45 TCTCGGT 35 0.0012472945 39.99279 43 CGGTGGT 40 0.002403213 34.99369 46 ATCTCGG 50 0.0071626594 27.994955 42 AATGGCT 50 0.0071626594 27.994955 22 AAGTGTA 65 7.878717E-4 26.918222 34 ATGTTGT 80 0.0026405612 21.871058 6 ATGCACG 95 0.0071398807 18.417734 70 GCCTGTC 125 0.0017383024 16.796972 59 CGACGAT 295 4.0563464E-10 16.607176 70 ACGATGC 205 2.1119107E-4 13.6560755 70 CCGACGA 400 2.9629518E-8 12.247793 69 GACGATG 245 9.4905775E-4 11.426512 69 TGCCGAC 480 3.1164745E-8 10.935529 70 GCCGACG 420 7.4220407E-7 10.831381 68 >>END_MODULE