FastQCFastQC Report
Wed 25 May 2016
SRR1780374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59466
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT33375.611609995627754No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT31565.307234386035718Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC10771.811118958732721No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC5380.9047186627652776No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG2910.48935526183028955No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT2900.48767362862812363No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1540.25897151313355526No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1330.2236572158880705No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1270.21356741667507484No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT1220.20515925066424512No Hit
CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC1130.19002455184475164No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1120.18834291864258568No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1100.1849796522382538No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT950.15975515420576464No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT820.13789392257760735No Hit
TTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC790.13284902297110956No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG710.119395957353782No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGTCTT650.10930615814078633No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTCTT640.10762452493862039No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGT640.10762452493862039No Hit
CTTATACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGT610.10257962533212256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAA150.002214512670.000011
CTTATAA150.002214512670.000011
AGACCTG150.002214512670.0000139
CTCGAAT150.002214512670.0000139
TTTACAC1800.056.3888852
ATTAGAC200.00691981752.533
TTAATAC407.3638876E-752.52
GGCACCG200.00691981752.59
TTAGACT200.00691981752.534
CAGGGAG200.00691981752.54
CGAGCAC200.00691981752.515
CTATACA950.051.578951
CTTTACA1900.051.578951
TTACACA2000.050.7499963
GAGTCAG305.8342825E-446.66666811
TCTTTAT659.751602E-943.0769231
CTTTATA701.8759238E-840.02
CTGATTG350.001246189840.054
TTCTGAT350.001246189840.052
CTTAATA556.592725E-638.1818161
Produced by FastQC (version 0.11.5)