##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780374_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59466 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.875592775703765 34.0 34.0 34.0 31.0 34.0 2 33.33303063935694 34.0 34.0 34.0 33.0 34.0 3 33.47751656408704 34.0 34.0 34.0 34.0 34.0 4 33.5103252278613 34.0 34.0 34.0 34.0 34.0 5 33.52184441529614 34.0 34.0 34.0 34.0 34.0 6 37.268573638717925 38.0 37.0 38.0 36.0 38.0 7 36.8680926916221 38.0 37.0 38.0 35.0 38.0 8 36.89607506810614 38.0 37.0 38.0 35.0 38.0 9 36.634127064204755 38.0 37.0 38.0 34.0 38.0 10-11 36.36148387313759 38.0 37.0 38.0 33.5 38.0 12-13 36.17211515824168 38.0 37.0 38.0 34.0 38.0 14-15 36.35470689133286 38.0 37.0 38.0 34.0 38.0 16-17 36.463559008509066 38.0 37.0 38.0 34.0 38.0 18-19 36.52766286617563 38.0 37.0 38.0 34.0 38.0 20-21 36.243609793831766 38.0 37.0 38.0 33.5 38.0 22-23 36.28825715534927 38.0 37.0 38.0 33.0 38.0 24-25 35.35564860592608 38.0 35.5 38.0 29.0 38.0 26-27 35.54251168735075 38.0 36.0 38.0 31.0 38.0 28-29 35.4702687249857 38.0 36.0 38.0 31.0 38.0 30-31 35.24644334577742 37.5 36.0 38.0 29.0 38.0 32-33 35.7830777250866 38.0 36.5 38.0 31.5 38.0 34-35 35.61407358826892 38.0 36.0 38.0 31.0 38.0 36-37 35.59628190899001 38.0 36.0 38.0 31.0 38.0 38-39 34.9729004809471 37.5 35.5 38.0 29.0 38.0 40-41 34.89683180304712 37.0 35.0 38.0 28.5 38.0 42-43 35.52920156055561 37.5 36.0 38.0 31.5 38.0 44-45 35.754641307637975 38.0 36.5 38.0 31.5 38.0 46-47 35.9967376315878 38.0 37.0 38.0 32.5 38.0 48-49 35.6836932028386 38.0 36.5 38.0 31.5 38.0 50-51 35.8269347189991 38.0 36.5 38.0 31.5 38.0 52-53 35.66606127871388 38.0 36.0 38.0 31.0 38.0 54-55 35.87272559109407 38.0 37.0 38.0 32.0 38.0 56-57 35.89029865805671 38.0 37.0 38.0 32.0 38.0 58-59 35.92168634177513 38.0 37.0 38.0 32.0 38.0 60-61 35.69466585948273 38.0 36.5 38.0 32.0 38.0 62-63 36.09216190764471 38.0 37.0 38.0 33.5 38.0 64-65 36.07521104496687 38.0 37.0 38.0 33.0 38.0 66-67 35.10915481115259 38.0 36.0 38.0 29.0 38.0 68-69 33.55558638549759 37.0 34.0 38.0 22.0 38.0 70-71 32.53357380688124 37.0 31.5 38.0 16.0 38.0 72-73 31.544630545185484 37.0 26.0 38.0 11.0 38.0 74-75 31.85035146133925 37.0 27.5 38.0 11.0 38.0 76 31.48580701577372 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3923370092987284 1101 2 0.4006729168871601 1101 3 -0.16725071768370725 1101 4 0.17451450009225056 1101 5 0.1516758815835999 1101 6 -0.9301912686820444 1101 7 -0.3044058255178683 1101 8 0.7195545223524462 1101 9 0.6483585197986059 1101 10-11 -0.06674022751350606 1101 12-13 0.6700758620744338 1101 14-15 0.8051311163049633 1101 16-17 -0.02861045821222774 1101 18-19 -0.49980302136916777 1101 20-21 0.32313160383424844 1101 22-23 0.9317383851487975 1101 24-25 -2.1981624443113574 1101 26-27 -0.6480988664453236 1101 28-29 0.6878259710677241 1101 30-31 1.3367452170757232 1101 32-33 0.4242235694611267 1101 34-35 0.6615777925651045 1101 36-37 0.4068787712529698 1101 38-39 0.8660295352784573 1101 40-41 1.2930914202754096 1101 42-43 0.7232319009092052 1101 44-45 0.2092859386292929 1101 46-47 0.6605309679336315 1101 48-49 -2.120496712790242 1101 50-51 -1.6458033405991088 1101 52-53 -1.385891269510914 1101 54-55 -0.7236994280357862 1101 56-57 -1.6899537037332735 1101 58-59 -1.4424273995231047 1101 60-61 -1.7795962801639718 1101 62-63 -1.25984890065196 1101 64-65 -0.7503418557022741 1101 66-67 -1.687295434790073 1101 68-69 -1.702712043230349 1101 70-71 -1.052738773048958 1101 72-73 -2.443290537886565 1101 74-75 -0.6869990487425106 1101 76 -0.0852381315747266 1102 1 -0.7999036184257733 1102 2 0.20642705812517903 1102 3 0.2949114444784513 1102 4 0.2401640205804867 1102 5 -0.05673737037803761 1102 6 0.018283012050304137 1102 7 0.1237110009860487 1102 8 0.44169924772297975 1102 9 0.4174082146547491 1102 10-11 0.4156704089293868 1102 12-13 0.4351368908451434 1102 14-15 -0.5883736788127365 1102 16-17 0.24924481641724583 1102 18-19 -0.2391229864955804 1102 20-21 0.9049973405386993 1102 22-23 0.3380810181043259 1102 24-25 -5.122704728531062 1102 26-27 -1.048142458424401 1102 28-29 -0.030417272175519372 1102 30-31 1.762551668688623 1102 32-33 0.7334868650147399 1102 34-35 0.9432037733846386 1102 36-37 -2.7453880116589744 1102 38-39 -0.422374998287367 1102 40-41 0.6121847071106359 1102 42-43 0.7841298956781628 1102 44-45 1.0482789639126509 1102 46-47 1.2238483175412966 1102 48-49 -3.5200172010204085 1102 50-51 -0.29643542429570147 1102 52-53 -0.31854166183872934 1102 54-55 1.0560520976834837 1102 56-57 1.0931979963539078 1102 58-59 -0.2785651501769877 1102 60-61 -5.605904039536245 1102 62-63 -2.1783536957696583 1102 64-65 0.03498944333870213 1102 66-67 1.5158649836929285 1102 68-69 -1.3364304390455217 1102 70-71 -0.6558338035633469 1102 72-73 -5.354646248435824 1102 74-75 -1.8155082030581156 1102 76 0.18219866005561158 1103 1 0.6911358080975276 1103 2 0.3434578070404726 1103 3 0.009531327519390231 1103 4 0.25140718224809433 1103 5 0.3964601395257574 1103 6 0.1544460001657555 1103 7 0.7484233194165384 1103 8 0.5478301664175618 1103 9 0.36236199695997584 1103 10-11 0.11061475905200524 1103 12-13 -0.1591283421297689 1103 14-15 -0.19640989839394507 1103 16-17 -0.3539621256691561 1103 18-19 0.7322975002050356 1103 20-21 -0.2931162229398936 1103 22-23 0.5262341967498685 1103 24-25 -2.856886958299029 1103 26-27 -0.360008144144075 1103 28-29 -0.38070966983633525 1103 30-31 1.091131181988004 1103 32-33 1.3336000512060266 1103 34-35 0.6415248448805784 1103 36-37 -0.29222823048561253 1103 38-39 0.28360500548550505 1103 40-41 -0.5076040119989713 1103 42-43 -0.07868844048482515 1103 44-45 1.0969301617998397 1103 46-47 0.5508620885279072 1103 48-49 -2.4269215586887682 1103 50-51 -0.5284293876988357 1103 52-53 0.3677439446506128 1103 54-55 0.09444108089419245 1103 56-57 0.20974204464668844 1103 58-59 -0.4310080854722145 1103 60-61 -4.232634845044643 1103 62-63 -1.4448694475146056 1103 64-65 0.26236163726437667 1103 66-67 -0.6294283571861499 1103 68-69 0.34598797257493175 1103 70-71 0.1765563115092874 1103 72-73 -4.7553065012183175 1103 74-75 -1.831222219281468 1103 76 -1.685562139793472 1104 1 0.4944037819537357 1104 2 0.4474137753927252 1104 3 0.2949114444784513 1104 4 -0.41467468909693395 1104 5 -0.5337772185753806 1104 6 0.16249553576946596 1104 7 0.36355919832950434 1104 8 0.5384734412713641 1104 9 0.9563076453947872 1104 10-11 -0.47218537854688947 1104 12-13 0.41995662519049404 1104 14-15 0.7397098126483641 1104 16-17 0.12894121110414858 1104 18-19 0.7043305239408468 1104 20-21 -0.3321943103151952 1104 22-23 0.10705635017264825 1104 24-25 0.31050210258848665 1104 26-27 0.9879106725053859 1104 28-29 1.716641551353888 1104 30-31 0.9396863935463173 1104 32-33 0.3663141894929183 1104 34-35 0.26606904852695124 1104 36-37 1.5381983261019343 1104 38-39 1.6139627626234514 1104 40-41 2.0150310069208857 1104 42-43 0.9914353985244588 1104 44-45 0.763459229567296 1104 46-47 0.8243227008430125 1104 48-49 0.23045718228130596 1104 50-51 0.5792761506568525 1104 52-53 0.182312228104351 1104 54-55 0.3880255322185846 1104 56-57 0.327733100718234 1104 58-59 -0.5169901976153142 1104 60-61 0.36686635894572817 1104 62-63 0.4056121486326276 1104 64-65 0.7051032953311065 1104 66-67 0.6284835795183596 1104 68-69 1.6918428057362647 1104 70-71 0.55687966892242 1104 72-73 0.6960178881865637 1104 74-75 -0.5544076337981529 1104 76 -0.5214066530373671 1105 1 0.8718439888057077 1105 2 0.31929483550908344 1105 3 0.33557078513779715 1105 4 -0.09159776602001557 1105 5 -0.21044173053400783 1105 6 0.269541431050655 1105 7 0.7412579701459237 1105 8 -0.9604276576297366 1105 9 0.6608325322726145 1105 10-11 0.4366381508648729 1105 12-13 -0.710715643717073 1105 14-15 0.3335688947427329 1105 16-17 0.12664644704467776 1105 18-19 0.673496651930499 1105 20-21 0.3446641253667764 1105 22-23 0.32218240059280845 1105 24-25 0.31059906445015883 1105 26-27 0.14214467379821372 1105 28-29 1.0668354750772266 1105 30-31 1.160921041251548 1105 32-33 0.4397789600165112 1105 34-35 -1.209561203573891 1105 36-37 0.059982424356618935 1105 38-39 -0.4651257958768724 1105 40-41 0.3834537606377566 1105 42-43 0.19806112573842682 1105 44-45 0.8007249050682645 1105 46-47 1.2191514015540577 1105 48-49 -0.45328554557907097 1105 50-51 0.9555851357893701 1105 52-53 0.5092676256479791 1105 54-55 0.9845349051985508 1105 56-57 0.7693943806148127 1105 58-59 0.5238630667673618 1105 60-61 1.0664461908784943 1105 62-63 -0.46355397935703735 1105 64-65 0.7133127329523106 1105 66-67 0.7590588865642474 1105 68-69 0.25396303844473067 1105 70-71 0.6424646721544889 1105 72-73 0.4865802669842445 1105 74-75 -0.8429984547419132 1105 76 -0.25683005316664875 1106 1 -1.7315083550529664 1106 2 0.12663938968908184 1106 3 0.1594684065037697 1106 4 0.15621138685243352 1106 5 0.3722540546934212 1106 6 0.3999489981670834 1106 7 -1.1348026780666203 1106 8 0.6004987577270597 1106 9 -0.5957400091100524 1106 10-11 0.29739764453575646 1106 12-13 1.4913639222865456 1106 14-15 -0.14688735786541685 1106 16-17 0.5999389772992316 1106 18-19 0.41389865499073863 1106 20-21 0.4735763523802703 1106 22-23 0.46368330475194597 1106 24-25 0.06671952626682298 1106 26-27 1.3672107469655543 1106 28-29 0.77527893035613 1106 30-31 1.2987705335314175 1106 32-33 -0.02527181199628359 1106 34-35 1.0734406047443699 1106 36-37 1.2536115800110963 1106 38-39 0.3632926211491423 1106 40-41 1.277354164031827 1106 42-43 0.9749424722400306 1106 44-45 1.1762291476611182 1106 46-47 0.9201529316695201 1106 48-49 1.2364139953862932 1106 50-51 0.5287176994282632 1106 52-53 0.8436674030857318 1106 54-55 -0.24529539108744558 1106 56-57 0.3189840314702863 1106 58-59 0.5213940168299587 1106 60-61 0.8648187044408928 1106 62-63 0.922626296063747 1106 64-65 -1.1303025125616628 1106 66-67 0.08250442836421712 1106 68-69 0.9050967148948601 1106 70-71 0.5848766905158698 1106 72-73 0.8265093252677254 1106 74-75 0.5121969827320036 1106 76 1.1738278964786417 1107 1 0.5626072795690007 1107 2 -0.18311086689661948 1107 3 0.14896549853251173 1107 4 0.16100098657874184 1107 5 0.16924344915116762 1107 6 0.25088981239903774 1107 7 0.6564049852929372 1107 8 0.24117614397406584 1107 9 0.1267368981769863 1107 10-11 0.7893408535675732 1107 12-13 0.23696775396633285 1107 14-15 0.39567165684550076 1107 16-17 0.8146327850310087 1107 18-19 0.9028996813335297 1107 20-21 0.4528613335639804 1107 22-23 0.00268433109474131 1107 24-25 0.10859431244540474 1107 26-27 -0.17107183941830328 1107 28-29 0.9506638089055599 1107 30-31 0.8907992711297723 1107 32-33 1.1289532991908473 1107 34-35 0.29873995472726733 1107 36-37 1.2136355280097249 1107 38-39 0.633597102846025 1107 40-41 0.6093076364916286 1107 42-43 0.9407994684767687 1107 44-45 0.835637289980653 1107 46-47 0.9517471841498448 1107 48-49 0.5889627466692247 1107 50-51 0.5670344972387298 1107 52-53 0.5046492710296206 1107 54-55 0.8742735449371892 1107 56-57 1.0539652151856416 1107 58-59 0.9886536815579774 1107 60-61 1.289998314430619 1107 62-63 0.6137997479966941 1107 64-65 0.8557392253788052 1107 66-67 -0.028511651006290606 1107 68-69 1.1425582270399204 1107 70-71 -0.18652255683274177 1107 72-73 -0.05612837572440199 1107 74-75 -1.3329449946884573 1107 76 0.13773484139824532 1108 1 -0.5013245093627887 1108 2 0.18880033001457974 1108 3 0.048482873049884745 1108 4 0.23532531090306463 1108 5 0.24514435005207247 1108 6 0.02412018562941398 1108 7 0.05032390421185795 1108 8 -0.4329551301572039 1108 9 -0.12030299886291118 1108 10-11 0.6777095794363035 1108 12-13 1.30714149914008 1108 14-15 0.6453820815559226 1108 16-17 -0.40883890094067254 1108 18-19 0.6057310841649368 1108 20-21 1.2757538689565209 1108 22-23 0.24480144821185945 1108 24-25 1.5976869765380712 1108 26-27 -0.024064117410581787 1108 28-29 -0.5762506055088465 1108 30-31 0.920078550409059 1108 32-33 1.162077494815044 1108 34-35 0.8410340345213498 1108 36-37 1.5739476258218303 1108 38-39 1.8370720063209305 1108 40-41 1.6265856287696252 1108 42-43 0.2651398803171787 1108 44-45 1.1649648318081915 1108 46-47 1.2887484711511377 1108 48-49 1.2532162859227611 1108 50-51 1.0336529013571365 1108 52-53 0.3941119479923003 1108 54-55 -0.3803918347281936 1108 56-57 0.6482863220067543 1108 58-59 0.6502366931409895 1108 60-61 1.245017619449925 1108 62-63 1.0595687312656779 1108 64-65 0.9010416706812521 1108 66-67 0.9392328792382401 1108 68-69 1.9762615732432707 1108 70-71 0.4709903131801241 1108 72-73 0.2010949189988942 1108 74-75 -0.3361303228737853 1108 76 -0.0027091740457692026 1109 1 -1.036833540561478 1109 2 0.30312709520340775 1109 3 0.29950914562788 1109 4 0.12900347182260674 1109 5 0.036811016718736767 1109 6 -0.5358141329255943 1109 7 -0.5143312682019356 1109 8 0.4947952803518234 1109 9 0.21344084350161552 1109 10-11 0.32456918534763446 1109 12-13 0.4538984777936079 1109 14-15 0.7781817531487007 1109 16-17 0.6846545309148269 1109 18-19 0.6303616260368585 1109 20-21 1.0110781710911638 1109 22-23 0.5393416780969105 1109 24-25 0.1726048747318245 1109 26-27 0.35586199096380255 1109 28-29 -0.8959345135548276 1109 30-31 0.3568601596044587 1109 32-33 0.7549551631237463 1109 34-35 -0.22926563707142833 1109 36-37 1.5438778392865231 1109 38-39 1.309567901230615 1109 40-41 0.11283357622446744 1109 42-43 1.0289945601201396 1109 44-45 0.5628507102975178 1109 46-47 1.1976154662250238 1109 48-49 0.9652442005368869 1109 50-51 0.506456678040216 1109 52-53 0.32504380349311646 1109 54-55 0.0069234362077637 1109 56-57 0.4989636619082347 1109 58-59 0.5652613236828614 1109 60-61 0.972850131765199 1109 62-63 0.17440863274350704 1109 64-65 1.1342108004021085 1109 66-67 0.37036998925466236 1109 68-69 0.3983674845733205 1109 70-71 0.9727349765627196 1109 72-73 0.5224208631696605 1109 74-75 -0.13682818822681853 1109 76 1.0516832725880576 1110 1 -0.7985514839849728 1110 2 -0.32905681284256616 1110 3 0.19551024687366692 1110 4 0.2877205204839015 1110 5 0.42303856162891407 1110 6 0.14530638465992496 1110 7 0.5705334850501842 1110 8 0.6474554771995074 1110 9 0.9365119426825643 1110 10-11 -0.6456971784764463 1110 12-13 0.5131936804916606 1110 14-15 0.9117422184250472 1110 16-17 0.5105544552041863 1110 18-19 0.21123436332090506 1110 20-21 0.8800987506217623 1110 22-23 -0.6296995497921287 1110 24-25 -1.223206949894788 1110 26-27 1.185409791913628 1110 28-29 0.7971276380041274 1110 30-31 -0.24109909430152499 1110 32-33 0.4428552250688256 1110 34-35 1.2294892669137099 1110 36-37 1.493340981966682 1110 38-39 1.3832653339513854 1110 40-41 -0.3286111205888034 1110 42-43 0.386486472846407 1110 44-45 0.6552521143858883 1110 46-47 0.7601627853798192 1110 48-49 1.468393075201348 1110 50-51 -0.1737002494840354 1110 52-53 0.6216746796717985 1110 54-55 -0.3388677400603868 1110 56-57 0.5374900573931285 1110 58-59 -0.3230310570728676 1110 60-61 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-0.15781759071514045 2116 50-51 -1.5162232571599645 2116 52-53 -0.2632266585912717 2116 54-55 -0.6135733623728115 2116 56-57 0.13112828429502343 2116 58-59 -2.018747007721906 2116 60-61 -1.923007308642113 2116 62-63 0.17124578463736384 2116 64-65 -1.7419752676377058 2116 66-67 -0.9509676640663258 2116 68-69 -0.9694678930398908 2116 70-71 1.3269546787921414 2116 72-73 0.9826345482734204 2116 74-75 1.2311036176823045 2116 76 0.11941450764166817 2117 1 0.8958542251447099 2117 2 0.3832719542807226 2117 3 0.27573336228667245 2117 4 -0.36398975759008323 2117 5 0.2354411537050396 2117 6 0.38191535849307456 2117 7 0.7095704795543227 2117 8 0.6658707015453373 2117 9 -0.9577133054512998 2117 10-11 -0.30308787653238767 2117 12-13 -0.9254905947933949 2117 14-15 -0.05593885777870611 2117 16-17 -1.118762253843471 2117 18-19 -0.9550973568004935 2117 20-21 0.2508435623681251 2117 22-23 0.0604407176182562 2117 24-25 -1.665767035203615 2117 26-27 -1.8760699882520697 2117 28-29 -1.4578145324494898 2117 30-31 -0.3139397144311218 2117 32-33 -2.700406197291933 2117 34-35 0.6779143345865819 2117 36-37 -0.9578935353001583 2117 38-39 -0.30872055727985526 2117 40-41 -0.3521325708389895 2117 42-43 0.18519043478554664 2117 44-45 -0.3739702627775898 2117 46-47 -0.5367570735324989 2117 48-49 -0.36966368798461247 2117 50-51 0.0999852562168897 2117 52-53 0.6856563054613147 2117 54-55 -0.30806632918351085 2117 56-57 0.48687895083911314 2117 58-59 -1.3380216238844511 2117 60-61 -0.3567436329962561 2117 62-63 -0.3109873678863124 2117 64-65 -0.8119394121628432 2117 66-67 -0.38199184352757243 2117 68-69 0.22874852692884673 2117 70-71 0.19281842798769588 2117 72-73 0.9750267520060625 2117 74-75 0.5041338649931468 2117 76 0.5449914130383817 2118 1 0.10268551569986784 2118 2 0.24331160821006392 2118 3 -0.45245697657417594 2118 4 0.22216741616622215 2118 5 0.42496891145557925 2118 6 0.19391968437627582 2118 7 0.649008114738173 2118 8 0.552947125481893 2118 9 -1.786029815905522 2118 10-11 -0.6061250070298669 2118 12-13 -0.8272569971005268 2118 14-15 0.650081329676226 2118 16-17 -2.8981872718679895 2118 18-19 -0.6284481138300535 2118 20-21 -0.5051045270334598 2118 22-23 0.7601523254048388 2118 24-25 1.0685516381922042 2118 26-27 -2.552259606132381 2118 28-29 -0.43370674585972324 2118 30-31 -0.9658863618716467 2118 32-33 -0.31990872072379517 2118 34-35 -0.20862135394983028 2118 36-37 -3.329874429316021 2118 38-39 0.2313389236141603 2118 40-41 -0.45424143889955104 2118 42-43 0.1384168226730722 2118 44-45 -2.291958727089053 2118 46-47 -0.20388937346039882 2118 48-49 0.25619247639895093 2118 50-51 -0.654160381850879 2118 52-53 -2.2503116109049444 2118 54-55 -1.2853103810941136 2118 56-57 0.3848151440618892 2118 58-59 -0.7647069158815682 2118 60-61 -0.855076365512474 2118 62-63 -1.2372671083333202 2118 64-65 -0.020167602501707904 2118 66-67 -0.027089175899604356 2118 68-69 -1.2486757701151276 2118 70-71 -0.2481512908299024 2118 72-73 1.4669157210039039 2118 74-75 0.7418771959232124 2118 76 1.0856366906158854 2119 1 0.7870954040571192 2119 2 -0.4578446916304415 2119 3 -0.012664313097303648 2119 4 -0.2343716587939042 2119 5 -0.27379504388732556 2119 6 -0.3730659615567333 2119 7 -0.2716457927578375 2119 8 0.20665525945318564 2119 9 -0.15385278241269873 2119 10-11 -0.17699821277149397 2119 12-13 -0.5619061199075404 2119 14-15 -0.11706380588996268 2119 16-17 -0.5270748316766003 2119 18-19 -0.5511953227614725 2119 20-21 -1.8581747024720485 2119 22-23 -0.18701673360632043 2119 24-25 0.6112151150662015 2119 26-27 0.5657627224162596 2119 28-29 0.10367363691538856 2119 30-31 0.8746240049545122 2119 32-33 0.6055298757674237 2119 34-35 0.2493132553005708 2119 36-37 -0.16928830491410451 2119 38-39 -1.4794864352375114 2119 40-41 -1.1396481374641425 2119 42-43 0.1063195339968388 2119 44-45 0.34380682315018163 2119 46-47 -0.6078965504938907 2119 48-49 -1.1207014629949867 2119 50-51 0.5555684857727172 2119 52-53 0.07674181812217284 2119 54-55 -0.5005217048580661 2119 56-57 -2.0835859723655403 2119 58-59 -0.4238975059932102 2119 60-61 -0.07965770522539373 2119 62-63 -0.6479529137559723 2119 64-65 -0.6022050825655043 2119 66-67 0.49145149395685905 2119 68-69 0.3536966381783273 2119 70-71 -1.391293236603424 2119 72-73 -0.07514966974569504 2119 74-75 -1.0866714484149078 2119 76 -0.6601767065133011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 4.0 22 10.0 23 22.0 24 55.0 25 104.0 26 220.0 27 381.0 28 627.0 29 960.0 30 1506.0 31 2308.0 32 3335.0 33 4896.0 34 6953.0 35 8644.0 36 11231.0 37 18210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.78118588773417 23.088823865738405 11.344297581811455 33.785692664715974 2 15.645915312951939 18.084283456092557 46.352537584502066 19.91726364645344 3 14.895906904785928 27.43080079373087 36.787407930582184 20.88588437090102 4 10.653146335721253 24.222244643998252 34.53401943967982 30.59058958060068 5 10.429489119833182 39.72185786836175 37.12037130461104 12.728281707194029 6 21.144855884034573 38.96007802778058 16.448054350385092 23.44701173779975 7 21.42737026199845 36.11139138331147 18.70144284128746 23.75979551340262 8 18.272626374735143 36.9690243164161 23.46887296942791 21.289476339420847 9 18.319712104395787 20.34271684660142 17.931254834695455 43.40631621430733 10-11 18.867924528301888 25.360710321864595 30.936165203645782 24.83519994618774 12-13 22.51286449399657 19.03861029832173 27.86298052668752 30.585544680994182 14-15 23.165338176436954 23.24521575353984 19.89792486462853 33.69152120539468 16-17 24.63844886153432 27.714155988295836 19.600275787845156 28.04711936232469 18-19 22.23035011603269 26.340261662126256 20.709312884673594 30.720075337167458 20-21 20.599165909931727 24.141526250294284 20.783304745568895 34.47600309420509 22-23 23.196448390677027 30.1895200618841 20.80348434399489 25.810547203443985 24-25 24.203746678774426 32.273904415968794 20.84888844045337 22.673460464803416 26-27 21.81666834829987 29.11916052870548 28.84337268355026 20.220798439444387 28-29 17.161066828103454 22.59778697070595 30.50818955369455 29.73295664749605 30-31 16.42451148555477 24.942824471126357 31.55500622204285 27.077657821276023 32-33 16.252144082332762 31.627316449735982 27.872229509299434 24.248309958631822 34-35 16.41358086974069 32.75989641139475 32.69263108330811 18.133891635556452 36-37 16.93572797901322 28.704637944371576 32.72962701375576 21.63000706285945 38-39 19.848316685164633 23.170383076043453 29.29320956512965 27.688090673662263 40-41 18.85531227928564 23.75979551340262 31.70383076043453 25.68106144687721 42-43 24.904987724077625 22.7239094608684 32.84818215450846 19.522920660545523 44-45 18.779638785188173 25.176571486227424 29.92045874953755 26.123330979046848 46-47 24.83015504658124 19.976961625130325 27.70742945548717 27.485453872801262 48-49 22.288366461507415 19.923149362661015 29.173813607775873 28.614670568055693 50-51 16.586789089563784 19.813002387919145 34.83755423267077 28.762654289846303 52-53 18.35670803484344 20.069451451249453 39.0727474523257 22.501093061581408 54-55 16.897891231964486 20.383916860054484 34.91154609356607 27.80664581441496 56-57 23.08209733292974 20.361214811825246 30.411495644570007 26.145192210675006 58-59 17.38640567719369 28.0218948642922 33.658729357952446 20.932970100561665 60-61 19.206773618538325 36.862240608078565 24.090236437628224 19.840749335754886 62-63 14.526788416910502 43.402952947903 21.901590825009247 20.168667810177244 64-65 14.177008710859987 44.8104799381159 20.75051289812666 20.261998452897455 66-67 14.371237345710153 44.12773685803653 20.083745333467864 21.417280462785456 68-69 14.302290384421351 41.56072377493022 20.567214879090574 23.569770961557865 70-71 14.848821175125282 38.63552281976255 21.464366192446104 25.05128981266606 72-73 15.020347761746208 35.4631217838765 22.095819459859413 27.420710994517876 74-75 15.35751521878048 34.86025628090001 22.49352571217166 27.28870278814785 76 14.904315070796759 35.27898294823933 22.160562338142803 27.65613964282111 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 1.0 15 2.5 16 2.5 17 2.0 18 3.5 19 13.0 20 23.5 21 26.0 22 35.5 23 56.0 24 72.0 25 77.0 26 107.0 27 182.0 28 246.5 29 266.0 30 324.0 31 421.5 32 541.0 33 621.0 34 761.0 35 1092.5 36 1611.0 37 1938.0 38 3003.5 39 5012.5 40 6035.5 41 5670.5 42 5226.0 43 4799.5 44 4178.5 45 3768.0 46 3552.0 47 3421.0 48 3186.0 49 2881.0 50 2680.0 51 2558.5 52 2179.0 53 1778.5 54 1636.0 55 1514.5 56 1198.5 57 881.0 58 758.0 59 636.5 60 424.0 61 293.5 62 254.0 63 219.5 64 144.5 65 84.0 66 44.5 67 25.0 68 25.0 69 19.5 70 8.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59466.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.78919046177648 #Duplication Level Percentage of deduplicated Percentage of total 1 65.38984171287964 29.941479164564626 2 17.753130853134525 16.258029798540342 3 7.671967387711631 10.538795277973968 4 3.4081310367622755 6.242222446439982 5 1.9537992581438908 4.47314431776141 6 1.16419993389401 3.198466350519625 7 0.7492012192882589 2.401372212692967 8 0.5508832494766609 2.017959842599132 9 0.30114950971390797 1.2410453031984663 >10 0.9768996290719454 6.538189890021188 >50 0.033052994968599654 1.0728819829818719 >100 0.033052994968599654 2.4417314095449503 >500 0.003672554996511073 0.9047186627652776 >1k 0.011017664989533217 12.729963340396191 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT 3337 5.611609995627754 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT 3156 5.307234386035718 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 1077 1.811118958732721 No Hit CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 538 0.9047186627652776 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 291 0.48935526183028955 No Hit TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT 290 0.48767362862812363 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 154 0.25897151313355526 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 133 0.2236572158880705 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 127 0.21356741667507484 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT 122 0.20515925066424512 No Hit CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 113 0.19002455184475164 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 112 0.18834291864258568 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 110 0.1849796522382538 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT 95 0.15975515420576464 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT 82 0.13789392257760735 No Hit TTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 79 0.13284902297110956 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 71 0.119395957353782 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGTCTT 65 0.10930615814078633 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTCTT 64 0.10762452493862039 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGT 64 0.10762452493862039 No Hit CTTATACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGT 61 0.10257962533212256 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0016816332021659436 0.0 4 0.0 0.0 0.0 0.0016816332021659436 0.0 5 0.0 0.0 0.0 0.0016816332021659436 0.0 6 0.0 0.0 0.0 0.0016816332021659436 0.0 7 0.0 0.0 0.0 0.0016816332021659436 0.0 8 0.0 0.0 0.0 0.0016816332021659436 0.0 9 0.0 0.0 0.0 0.006726532808663774 0.0 10 0.0 0.0 0.0 0.010089799212995661 0.0 11 0.0 0.0 0.0 0.013453065617327549 0.0 12 0.0 0.0 0.0 0.015134698819493492 0.0 13 0.0 0.0 0.0 0.015134698819493492 0.0 14 0.0 0.0 0.0 0.015134698819493492 0.0 15 0.0 0.0 0.0 0.01849796522382538 0.0 16 0.0 0.0 0.0 0.020179598425991322 0.0 17 0.0 0.0 0.0 0.020179598425991322 0.0 18 0.0 0.0 0.0 0.021861231628157268 0.0 19 0.0 0.0 0.0 0.02858776443682104 0.0 20 0.0 0.0 0.0 0.035314297245484814 0.0 21 0.0 0.0 0.0 0.035314297245484814 0.0 22 0.0 0.0 0.0 0.035314297245484814 0.0 23 0.0 0.0 0.0 0.035314297245484814 0.0 24 0.0 0.0 0.0 0.035314297245484814 0.0 25 0.0 0.0 0.0 0.04204083005414859 0.0 26 0.0 0.0 0.0 0.05549389567147614 0.0 27 0.0 0.0 0.0 0.08912655971479501 0.0 28 0.0 0.0 0.0 0.12444085696027983 0.0 29 0.0 0.0 0.0 0.14125718898193926 0.0 30 0.0 0.0 0.0 0.16143678740793058 0.0 31 0.0 0.0 0.0 0.20011435105774728 0.0 32 0.0 0.0 0.0 0.2623347795378872 0.0 33 0.0 0.0 0.0 0.34137154003968656 0.0 34 0.0 0.0 0.0 0.43049809975448156 0.0 35 0.0 0.0 0.0 0.5179430262671106 0.0 36 0.0 0.0 0.0 0.7062859449096963 0.0 37 0.0 0.0 0.0 1.0190697205125618 0.0 38 0.0 0.0 0.0 1.4125718898193926 0.0 39 0.0 0.0 0.0 1.7842128274980662 0.0 40 0.0 0.0 0.0 2.320653818989002 0.0 41 0.0 0.0 0.0 2.9024989069384186 0.0 42 0.0 0.0 0.0 3.637372616284936 0.0 43 0.0 0.0 0.0 4.489960649783069 0.0 44 0.0 0.0 0.0 5.223152725927421 0.0 45 0.0 0.0 0.0 5.956344802071772 0.0 46 0.0 0.0 0.0 6.775300171526586 0.0 47 0.0 0.0 0.0 7.5286718460969295 0.0 48 0.0 0.0 0.0 8.245047590219622 0.0 49 0.0 0.0 0.0 8.79326001412572 0.0 50 0.0 0.0 0.0 9.361652036457809 0.0 51 0.0 0.0 0.0 10.032623684122019 0.0 52 0.0 0.0 0.0 10.854942319981166 0.0 53 0.0 0.0 0.0 11.640265025392662 0.0 54 0.0 0.0 0.0 12.42054283119766 0.0 55 0.0 0.0 0.0 13.158779806948509 0.0 56 0.0 0.0 0.0 13.82975145461272 0.0 57 0.0 0.0 0.0 14.414959808966469 0.0 58 0.0 0.0 0.0 15.06070695859819 0.0 59 0.0 0.0 0.0 15.49624995795917 0.0 60 0.0 0.0 0.0 16.03941748225877 0.0 61 0.0 0.0 0.0 16.532136010493392 0.0 62 0.0 0.0 0.0 16.994585141089026 0.0 63 0.0 0.0 0.0 17.56129553021895 0.0 64 0.0 0.0 0.0 18.26085494231998 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGAA 15 0.0022145126 70.00001 1 CTTATAA 15 0.0022145126 70.00001 1 AGACCTG 15 0.0022145126 70.00001 39 CTCGAAT 15 0.0022145126 70.00001 39 TTTACAC 180 0.0 56.388885 2 ATTAGAC 20 0.006919817 52.5 33 TTAATAC 40 7.3638876E-7 52.5 2 GGCACCG 20 0.006919817 52.5 9 TTAGACT 20 0.006919817 52.5 34 CAGGGAG 20 0.006919817 52.5 4 CGAGCAC 20 0.006919817 52.5 15 CTATACA 95 0.0 51.57895 1 CTTTACA 190 0.0 51.57895 1 TTACACA 200 0.0 50.749996 3 GAGTCAG 30 5.8342825E-4 46.666668 11 TCTTTAT 65 9.751602E-9 43.076923 1 CTTTATA 70 1.8759238E-8 40.0 2 CTGATTG 35 0.0012461898 40.0 54 TTCTGAT 35 0.0012461898 40.0 52 CTTAATA 55 6.592725E-6 38.181816 1 >>END_MODULE