FastQCFastQC Report
Wed 25 May 2016
SRR1780373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396315
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAA300.005757180529.3158281
ATCGATA407.017122E-427.50442123
CTTTATA609.824564E-625.6351682
CGGAGTG959.658834E-1025.45526932
TAATCGA450.001381945724.48859621
AAAAAGC901.481385E-824.482415
AGAGTTA450.001403484724.42368943
GAGTTAC450.001403484724.42368944
TCTCGGG1102.0190782E-1023.97962243
GTAATCG752.0263742E-623.50905220
TCGGAGT852.3607936E-723.27727731
GCGTGTA803.5924659E-622.009134
AATCGAT602.8649348E-422.00353822
CGAAACT956.8650115E-720.83492925
CGACGAA3700.020.81415722
TAGAGCC755.512617E-520.5081334
CTTAGAA654.9650646E-420.282772
TCAGGAA550.004431838620.03612520
CCCATAG550.004510325419.9754542
AAACTCG1001.1259617E-619.78818327
Produced by FastQC (version 0.11.5)