FastQCFastQC Report
Wed 25 May 2016
SRR1780373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396315
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT277787.009071067206642No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT245016.182203550206275RNA PCR Primer, Index 24 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC77971.9673744370008703No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC42011.0600153917969293No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG27910.7042377906463293No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT22830.5760569244161841No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC15390.38832746678778246No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC12470.3146487011594313No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG10250.2586326533186985No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC9060.22860603307974717No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG8020.20236428093814265No Hit
CTTAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC7380.18621551038946293No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC5720.14432963677882493No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG5020.12666691899120647No Hit
CTTATCACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC4230.10673328034517997No Hit
CTTATTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG4200.10597630672571062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT26050.042.73136544
GCCGTCT29000.038.38455643
TTTACAC8950.037.607322
CTTATAA1150.036.3829881
CTTTACA9400.035.843151
CTATACA5250.035.6535031
ATTCCGC250.00234993235.20292712
TTACACA10050.033.27223
GCCGCTT401.8159679E-533.00274311
GCACTAA353.1958142E-431.4589711
CATCGTT353.2148015E-431.42721732
ATTACAC857.2759576E-1231.0574874
CTTATAC41200.030.3059861
TTATACA41600.029.7727572
CCGCTTT454.06042E-529.33577312
TCACGGA300.005741621429.33207343
TAGCCCG454.0639177E-529.3320735
CGCTACG300.005741621429.33207316
GCGGCGT454.0639177E-529.33207320
TGCAGAC300.005741621429.3320735
Produced by FastQC (version 0.11.5)