##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780372_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10871 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.039922730199613 12.0 12.0 23.0 12.0 32.0 2 18.41182963848772 12.0 11.0 23.0 11.0 32.0 3 19.123539692760556 21.0 11.0 23.0 11.0 33.0 4 20.187379265936897 21.0 11.0 31.0 11.0 34.0 5 20.29307331432251 21.0 11.0 31.0 11.0 34.0 6 19.867261521479165 11.0 11.0 28.0 11.0 35.0 7 21.954649986201822 22.0 11.0 31.0 11.0 36.0 8 20.50160978750805 21.0 11.0 31.0 11.0 35.0 9 21.494802686045443 21.0 12.0 31.0 10.0 36.0 10-11 20.55054732775274 21.0 11.0 29.5 10.5 35.0 12-13 20.634394259957684 21.0 11.0 29.5 11.0 34.0 14-15 20.456305767638675 21.0 11.0 29.5 11.0 34.0 16-17 20.024468770122343 21.0 11.0 28.0 11.0 34.0 18-19 20.251494802686047 21.0 11.0 28.0 11.0 34.0 20-21 20.362386165026216 16.0 11.0 29.0 11.0 34.0 22-23 19.823843252690644 16.0 11.0 29.0 10.5 34.0 24-25 19.986753748505194 16.0 11.0 29.0 10.5 34.0 26-27 19.501839757152055 16.0 11.0 27.0 10.5 34.0 28-29 19.17919234661025 11.0 11.0 26.0 11.0 34.0 30-31 19.091711894029988 11.0 11.0 26.0 11.0 34.0 32-33 19.423098151044062 16.0 11.0 26.5 11.0 34.0 34-35 19.633336399595255 16.0 11.0 27.0 11.0 34.0 36-37 19.452074326188942 16.0 11.0 26.5 11.0 34.0 38-39 18.788013982154354 11.0 11.0 26.0 11.0 34.0 40-41 19.175466838377336 11.0 11.0 26.0 11.0 34.0 42-43 19.41684297672707 16.0 11.0 26.5 11.0 34.0 44-45 19.074556158587065 11.0 11.0 26.0 11.0 34.0 46-47 19.257795970931838 11.0 11.0 26.0 11.0 34.0 48-49 19.472863582007175 16.0 11.0 26.0 11.0 34.0 50-51 19.43947198969736 15.0 11.0 26.0 11.0 34.0 52-53 19.466332444117377 15.0 11.0 26.0 11.0 34.0 54-55 19.50749701039463 21.0 11.0 26.0 11.0 34.0 56-57 18.89895133842333 15.0 11.0 26.0 11.0 33.5 58-59 18.960353233373198 16.0 11.0 26.0 11.0 33.5 60-61 19.18779321129611 19.0 11.0 26.0 11.0 33.0 62-63 18.85061171925306 15.0 11.0 26.0 11.0 33.0 64-65 19.10390028516236 15.0 11.0 26.0 11.0 33.0 66-67 19.402814828442644 19.0 11.0 26.0 11.0 33.5 68-69 19.029850059792107 15.0 11.0 25.5 11.0 33.0 70-71 19.12110201453408 19.0 11.0 26.0 11.0 33.0 72-73 19.097783092631772 19.0 11.0 25.5 11.0 33.0 74-75 18.79210744181768 15.0 11.0 25.0 11.0 33.0 76 18.198417808849232 11.0 11.0 24.0 11.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.8218336848176193 1101 2 0.11944703336817497 1101 3 -0.5997743135155105 1101 4 0.682921154530078 1101 5 -1.3698007091143118 1101 6 1.3602143752192113 1101 7 0.015854641157478255 1101 8 -2.5240145210383886 1101 9 -1.5222678860435614 1101 10-11 -1.022095336839854 1101 12-13 -1.655851711059988 1101 14-15 -0.6415245737917097 1101 16-17 -0.9848145397259316 1101 18-19 -3.46575789312552 1101 20-21 -0.03521378526015084 1101 22-23 -2.3769596912874427 1101 24-25 -0.01253456178731227 1101 26-27 -1.6564214377893478 1101 28-29 -5.6723995938996055 1101 30-31 -2.8103195133218914 1101 32-33 -1.480038613377502 1101 34-35 -1.3374343163029714 1101 36-37 -1.793663393518905 1101 38-39 -1.0839979933995103 1101 40-41 -1.8685453244256642 1101 42-43 -1.2968792799463849 1101 44-45 -2.9502710487908494 1101 46-47 -2.201182306161744 1101 48-49 -1.77356075365282 1101 50-51 -1.2543554271627038 1101 52-53 -3.1908708487141446 1101 54-55 0.9879073118526307 1101 56-57 -4.004503581745293 1101 58-59 -0.7041356741552853 1101 60-61 -2.960293432368651 1101 62-63 -2.4868091233729253 1101 64-65 -1.2935626090941454 1101 66-67 -1.2259149515584369 1101 68-69 -0.07966040808748076 1101 70-71 -1.217913081815869 1101 72-73 -2.2569862746787344 1101 74-75 -2.010793155998698 1101 76 -1.5869735591405885 1102 1 0.5750388130227471 1102 2 0.20278036670150712 1102 3 -0.04208200582320387 1102 4 -1.8010532044442833 1102 5 1.460327496013896 1102 6 -0.636580496575661 1102 7 -0.4040171537143138 1102 8 -0.7708093928332609 1102 9 -0.46457557835125485 1102 10-11 1.234314919570405 1102 12-13 -0.35617222388049896 1102 14-15 0.7847574774903414 1102 16-17 1.1337752038638094 1102 18-19 0.6271908248231988 1102 20-21 2.849401599355236 1102 22-23 3.313745436917685 1102 24-25 1.473042361289611 1102 26-27 0.3211426647747544 1102 28-29 -1.7685534400534522 1102 30-31 -0.5779477184500976 1102 32-33 0.8452818994430125 1102 34-35 -0.005703547072204884 1102 36-37 -1.3930223678778795 1102 38-39 -1.2955364549379702 1102 40-41 1.19715980377946 1102 42-43 1.5460694380023376 1102 44-45 -2.6794377154575137 1102 46-47 0.6161253861459492 1102 48-49 -2.2126633177553856 1102 50-51 -2.307240042547317 1102 52-53 2.046308638465341 1102 54-55 -0.701195252249935 1102 56-57 0.2695348797931665 1102 58-59 -0.22977669979631088 1102 60-61 0.18874502916980873 1102 62-63 0.5724857484219505 1102 64-65 1.4099630319314933 1102 66-67 -1.5031585413020245 1102 68-69 -2.0732501516772217 1102 70-71 0.219586918184131 1102 72-73 -1.7329478131402745 1102 74-75 -0.8024598226653623 1102 76 -0.8978709950380264 1103 1 1.7586616418068672 1103 2 4.380199721540215 1103 3 2.2792579445490055 1103 4 -0.04422938310433011 1103 5 0.2605756349717119 1103 6 0.111558461240719 1103 7 0.12203743685640589 1103 8 0.3160392424024714 1103 9 1.0986998558919225 1103 10-11 0.012850899719289544 1103 12-13 0.39320742872495984 1103 14-15 -2.0675998426089173 1103 16-17 1.3955618043600886 1103 18-19 0.8554786660142639 1103 20-21 0.01317331151404133 1103 22-23 0.11833600763728924 1103 24-25 0.18706221240623577 1103 26-27 1.551911895543988 1103 28-29 0.3114713738423305 1103 30-31 0.5330944651727307 1103 32-33 -0.08958162413018655 1103 34-35 2.039581812729285 1103 36-37 1.4227344559434592 1103 38-39 3.1095503936972655 1103 40-41 1.1711052132087438 1103 42-43 0.6614540533869508 1103 44-45 2.5947558329295823 1103 46-47 1.0367209196447078 1103 48-49 0.26810591301384434 1103 50-51 0.3242736050953603 1103 52-53 1.0779463555869313 1103 54-55 2.6989288172289747 1103 56-57 -1.3452293881969055 1103 58-59 -1.4245657816821655 1103 60-61 0.11430334182489688 1103 62-63 0.5824113067346026 1103 64-65 0.6345287887553148 1103 66-67 1.1537893495168348 1103 68-69 1.753672925245855 1103 70-71 -1.3529937269771573 1103 72-73 0.30551372532126564 1103 74-75 -0.028266274278266224 1103 76 -0.16089829032338443 1104 1 1.2697503514842872 1104 2 -1.3284696332984929 1104 3 1.3689756864844895 1104 4 -2.48374551213659 1104 5 -3.671884042447644 1104 6 0.8706310418858791 1104 7 -2.036228692175854 1104 8 -1.3260978543717208 1104 9 -3.3035178860435614 1104 10-11 0.06123799649348172 1104 12-13 -0.9006433777266523 1104 14-15 1.5720170928749546 1104 16-17 -1.4275228730592673 1104 18-19 1.570700440207812 1104 20-21 -0.7383387852601508 1104 22-23 -0.9915430246207748 1104 24-25 0.6280904382126877 1104 26-27 0.3487868955439879 1104 28-29 0.9682254061003945 1104 30-31 2.8198888200114425 1104 32-33 0.35850305328916576 1104 34-35 -0.7436843163029714 1104 36-37 0.5865449398144271 1104 38-39 1.3847520066004897 1104 40-41 0.25124634224100006 1104 42-43 -1.1041709466130492 1104 44-45 -0.6325627154575137 1104 46-47 -1.7636823061617442 1104 48-49 -0.10689408698615566 1104 50-51 -0.9418554271627038 1104 52-53 -0.12316251538081247 1104 54-55 -0.236051021480705 1104 56-57 -0.5670035817452934 1104 58-59 -0.3968440074886175 1104 60-61 -0.46029343236865117 1104 62-63 -1.4191007900395896 1104 64-65 -1.0852292757608133 1104 66-67 0.6855433817748988 1104 68-69 -0.32445207475414506 1104 70-71 1.485211918184131 1104 72-73 1.2117637253212656 1104 74-75 2.4475401773346377 1104 76 -0.4098902258072563 1105 1 -1.5436425056585712 1105 2 -3.297219633298493 1105 3 -0.6783457420869397 1105 4 -0.8230312264223052 1105 5 -0.9209911853047856 1105 6 -1.8838332438284056 1105 7 0.14323559353843152 1105 8 -0.7752049972288653 1105 9 0.31344639967072396 1105 10-11 -2.0101905749350877 1105 12-13 -1.2243040920123676 1105 14-15 0.057284950017812974 1105 16-17 -1.7145764444878395 1105 18-19 -1.4431388455064713 1105 20-21 -1.2066423566887217 1105 22-23 -3.2406501674779165 1105 24-25 -1.0857488475015984 1105 26-27 -2.905230961598871 1105 28-29 -0.6723995938996055 1105 30-31 -0.4180576085599874 1105 32-33 -0.6080148038536919 1105 34-35 -0.439220030588686 1105 36-37 0.1932413683858556 1105 38-39 -2.2982837076852256 1105 40-41 -1.9500929434732868 1105 42-43 -0.7099745180416193 1105 44-45 0.006276570256769531 1105 46-47 1.1095319795525391 1105 48-49 -1.4890369441290154 1105 50-51 -0.8061411414484176 1105 52-53 -0.3718232296665249 1105 54-55 1.0081454070907228 1105 56-57 0.05799641825470658 1105 58-59 -1.066040436060046 1105 60-61 0.2414922819170613 1105 62-63 0.6054527813889798 1105 64-65 -2.4977292757608147 1105 66-67 -1.2872244753679567 1105 68-69 -2.3177556461827145 1105 70-71 -1.8161273675301537 1105 72-73 1.948370868178408 1105 74-75 2.6975401773346377 1105 76 -0.21435451152154172 1106 1 -0.6364996485157128 1106 2 -0.8329339190127776 1106 3 0.6859399721987742 1106 4 -1.1801740835651628 1106 5 -1.8638483281619287 1106 6 0.11616675617159444 1106 7 -1.9067644064615692 1106 8 0.053366431342563914 1106 9 0.7991606853850115 1106 10-11 1.1326665679220511 1106 12-13 -0.7421612348695099 1106 14-15 -2.7070007642678995 1106 16-17 0.646137841226448 1106 18-19 -1.4359959883636186 1106 20-21 -0.5173566424030085 1106 22-23 1.3343498325220828 1106 24-25 -0.45003456178731227 1106 26-27 -1.448088104456012 1106 28-29 0.7740289775289639 1106 30-31 0.10337096286858483 1106 32-33 -3.0187290895679766 1106 34-35 -1.2213628877315408 1106 36-37 -0.6389014887570035 1106 38-39 -0.494712279113795 1106 40-41 -1.6929500863304305 1106 42-43 -1.0885459466130492 1106 44-45 -0.7330091440289443 1106 46-47 -0.8261823061617442 1106 48-49 0.08953448444241374 1106 50-51 1.468858858551581 1106 52-53 -0.10753751538081247 1106 54-55 -1.3097117357664203 1106 56-57 0.5579964182547066 1106 58-59 2.69467384965424 1106 60-61 1.4772065676313488 1106 62-63 0.2661670671032681 1106 64-65 0.745127867096329 1106 66-67 -2.062224475367959 1106 68-69 -1.4248985033255757 1106 70-71 1.9338726324698463 1106 72-73 -0.03377198896444966 1106 74-75 -1.106031251236793 1106 76 -0.45006879723582927 1107 1 -1.219832981849045 1107 2 -1.0547953908742507 1107 3 -0.8800773438185416 1107 4 -1.9269273303184065 1107 5 1.4824720181584183 1107 6 -2.0223613823565465 1107 7 0.9666122169150562 1107 8 -3.808105430129295 1107 9 -2.6510557648314403 1107 10-11 -2.6357317004762137 1107 12-13 -1.055472923181199 1107 14-15 -1.933191240458381 1107 16-17 -2.2310266609380562 1107 18-19 -2.727121529489157 1107 20-21 -1.3988501488965142 1107 22-23 -2.322035448863197 1107 24-25 -1.95761031936307 1107 26-27 -2.02384568021359 1107 28-29 -1.687551109051121 1107 30-31 -1.3197892102915887 1107 32-33 -3.6599628558017443 1107 34-35 -1.7219040132726668 1107 36-37 -2.047451272306784 1107 38-39 -3.0688464782479947 1107 40-41 -3.4461968395771816 1107 42-43 -1.6112732193403225 1107 44-45 -1.543074079093877 1107 46-47 -2.015576245555682 1107 48-49 -0.47431832941039787 1107 50-51 -2.010037245344524 1107 52-53 -0.49390115174444915 1107 54-55 0.49169519064050604 1107 56-57 -1.7753369150786291 1107 58-59 -1.5071659771855863 1107 60-61 -2.409157068732288 1107 62-63 -1.859915183978984 1107 64-65 -1.3674262454577821 1107 66-67 -1.5422028303463122 1107 68-69 -1.3978422262692938 1107 70-71 -0.8637464151492047 1107 72-73 -1.9066074867999454 1107 74-75 -1.2721567923623311 1107 76 -1.5225796197466508 1108 1 2.0252650573666386 1108 2 -1.1501608097690799 1108 3 -0.555656666456688 1108 4 -0.3440396297836479 1108 5 -1.195781101271173 1108 6 -1.2745895463494143 1108 7 1.3222271901770881 1108 8 -1.8205831484893693 1108 9 -2.184032591925913 1108 10-11 0.2671203494346557 1108 12-13 -0.39237131890312327 1108 14-15 -0.011622613007400417 1108 16-17 0.9980285975289682 1108 18-19 0.19845779314898593 1108 20-21 0.45008033238690714 1108 22-23 -0.5007342010913618 1108 24-25 0.5205536735068037 1108 26-27 1.7872060131910459 1108 28-29 -0.12828194684078298 1108 30-31 0.66639617295262 1108 32-33 -0.5596954761226023 1108 34-35 -1.5984637280676743 1108 36-37 0.2529052339320721 1108 38-39 -1.6281156404583328 1108 40-41 1.235621342241 1108 42-43 -0.05913418190716513 1108 44-45 -1.0764965389869268 1108 46-47 -1.2967705414558601 1108 48-49 -1.9671882046332136 1108 50-51 1.0140269257784702 1108 52-53 -0.681066927145519 1108 54-55 -0.352778962657176 1108 56-57 0.3668199476664711 1108 58-59 1.3448839336878535 1108 60-61 -0.5080875500157092 1108 62-63 -0.4218581429807635 1108 64-65 -0.5006704522314003 1108 66-67 -0.4918463241074562 1108 68-69 -1.275738839460029 1108 70-71 -2.16276602299234 1108 72-73 -0.7091921570316764 1108 74-75 1.4622460596875797 1108 76 -1.5109931669837273 1109 1 -0.9489996485157128 1109 2 -2.609719633298493 1109 3 0.8689756864844895 1109 4 1.39125448786341 1109 5 1.109365957552356 1109 6 -2.629368958114121 1109 7 2.213771307824146 1109 8 -1.8260978543717208 1109 9 0.6027321139564386 1109 10-11 -0.6575120035065183 1109 12-13 1.1774816222733477 1109 14-15 0.2438920928749546 1109 16-17 -0.4900228730592673 1109 18-19 2.586325440207812 1109 20-21 0.19916121473984916 1109 22-23 1.4303319753792252 1109 24-25 0.9405904382126877 1109 26-27 -1.791838104456012 1109 28-29 2.0932254061003945 1109 30-31 0.6167638200114425 1109 32-33 -0.20399694671083424 1109 34-35 0.9594406836970286 1109 36-37 -0.11658006018557288 1109 38-39 1.2285020066004897 1109 40-41 1.516871342241 1109 42-43 1.5677040533869508 1109 44-45 2.7736872845424863 1109 46-47 0.8456926938382558 1109 48-49 1.1118559130138443 1109 50-51 0.16751957283729624 1109 52-53 0.5643374846191875 1109 54-55 2.513948978519295 1109 56-57 1.4173714182547066 1109 58-59 1.0719059925113825 1109 60-61 1.1334565676313488 1109 62-63 -0.044100790039589555 1109 64-65 -1.0696042757608133 1109 66-67 0.4511683817748988 1109 68-69 0.44117292524585494 1109 70-71 1.657086918184131 1109 72-73 0.9148887253212656 1109 74-75 1.6819151773346377 1109 76 -0.5036402258072563 1110 1 0.26501550299943943 1110 2 -0.11540145148030945 1110 3 0.029013565272368425 1110 4 -1.1693515727426522 1110 5 3.391562927249325 1110 6 -1.2647856247807887 1110 7 0.5120667623695994 1110 8 -3.2020448240686896 1110 9 1.0459139321382551 1110 10-11 -0.8327013974459128 1110 12-13 2.4142240465157734 1110 14-15 1.2789299716628335 1110 16-17 -0.39769332760472054 1110 18-19 2.0001511977835698 1110 20-21 0.4950892450428803 1110 22-23 -1.9129445397722904 1110 24-25 -1.0182163799691324 1110 26-27 1.551911895543988 1110 28-29 0.03972161822160558 1110 30-31 0.3923320018296259 1110 32-33 -2.67511437095326 1110 34-35 -1.130994922363577 1110 36-37 1.4373972125417005 1110 38-39 -0.19005859946011938 1110 40-41 -1.0977119910923356 1110 42-43 1.2372116291445288 1110 44-45 0.04783501181521288 1110 46-47 -0.1216368516162909 1110 48-49 -0.35310620819827676 1110 50-51 -2.6009463362536103 1110 52-53 -2.5393556971989923 1110 54-55 -1.220426021480705 1110 56-57 0.3610267212850111 1110 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62-63 2.16365383959004 2116 64-65 -1.71254409057563 2116 66-67 0.4847331965897155 2116 68-69 0.3092284808014121 2116 70-71 0.2658832144804286 2116 72-73 -1.5648566450491046 2116 74-75 1.8086512884457484 2116 76 2.8343227371557056 2117 1 2.613500351484287 2117 2 2.077780366701507 2117 3 0.23355901981782168 2117 4 1.307921154530078 2117 5 2.088532624219024 2117 6 3.2352143752192113 2117 7 1.099187974490814 2117 8 1.1426521456282792 2117 9 -3.6889345527102293 2117 10-11 -1.0220953368398504 2117 12-13 1.7399816222733477 2117 14-15 3.6501420928749546 2117 16-17 -0.23481453972593158 2117 18-19 4.221742106874476 2117 20-21 -0.681047118593483 2117 22-23 3.414706975379225 2117 24-25 1.4041321048793556 2117 26-27 0.51024522887732 2117 28-29 1.4317670727670624 2117 30-31 1.7521804866781068 2117 32-33 0.37412805328916576 2117 34-35 1.7458990170303643 2117 36-37 1.9355032731477628 2117 38-39 1.6660020066004897 2117 40-41 3.4647880089076644 2117 42-43 2.4947873867202865 2117 44-45 0.34139561787582196 2117 46-47 -0.6386823061617442 2117 48-49 -0.5443940869861557 2117 50-51 -1.150188760496036 2117 52-53 -0.2742041820474803 2117 54-55 0.1337406451859593 2117 56-57 1.9329964182547066 2117 58-59 2.1500309925113825 2117 60-61 -0.251960099035319 2117 62-63 1.2215242099604104 2117 64-65 3.4772707242391867 2117 66-67 1.2115850484415631 2117 68-69 3.566172925245855 2117 70-71 0.5737535848507989 2117 72-73 0.7013470586546013 2117 74-75 1.2600401773346377 2117 76 3.5380264408594115 2118 1 1.7563574943414295 2118 2 0.7742089381300801 2118 3 0.18593997219877423 2118 4 1.5698259164348372 2118 5 1.2790088146952137 2118 6 -0.9552618152569785 2118 7 0.45037845068128846 2118 8 2.9462235741997063 2118 9 0.9777321139564386 2118 10-11 -0.45661914636366063 2118 12-13 -1.5100183777266523 2118 14-15 -0.20700076426789948 2118 16-17 1.199709269797875 2118 18-19 0.17114686877924257 2118 20-21 1.4112147861684186 2118 22-23 0.3522069753792252 2118 24-25 0.9785368667841183 2118 26-27 0.8019118955439879 2118 28-29 1.8276004061003945 2118 30-31 1.5676566771543001 2118 32-33 1.838413767574881 2118 34-35 0.3143513979827439 2118 36-37 -0.3531872030427152 2118 38-39 -0.8339979933995103 2118 40-41 -0.31795008633043054 2118 42-43 -1.2849745180416186 2118 44-45 1.7848479988282016 2118 46-47 0.5845319795525405 2118 48-49 1.1609630558709902 2118 50-51 -1.9954268557341308 2118 52-53 0.8924624846191875 2118 54-55 0.7974311213764409 2118 56-57 0.6472821325404219 2118 58-59 0.08753099251138252 2118 60-61 1.1736351390599182 2118 62-63 -1.7874043614681625 2118 64-65 0.9236992956677561 2118 66-67 1.7056326674891835 2118 68-69 -1.3356127890398604 2118 70-71 0.719586918184131 2118 72-73 3.6090851538926962 2118 74-75 0.3939687487632071 2118 76 0.3713597741927437 2119 1 -1.594832981849045 2119 2 -0.630552966631825 2119 3 -0.8914409801821783 2119 4 0.5995878211967423 2119 5 1.046865957552356 2119 6 0.8602143752192113 2119 7 -1.9841453588425217 2119 8 1.8509854789616114 2119 9 3.5193987806231064 2119 10-11 2.0820713298268174 2119 12-13 0.6149816222733477 2119 14-15 3.879308759541619 2119 16-17 2.4318521269407327 2119 18-19 2.680075440207812 2119 20-21 -0.493547118593483 2119 22-23 1.7272069753792252 2119 24-25 0.966632104879352 2119 26-27 -0.8647547711226764 2119 28-29 -0.9223995938996055 2119 30-31 0.4396804866781068 2119 32-33 1.47829471995583 2119 34-35 -0.8166009829696357 2119 36-37 -0.7519967268522372 2119 38-39 0.7285020066004897 2119 40-41 1.673121342241 2119 42-43 -0.442712613279717 2119 44-45 2.674728951209154 2119 46-47 1.3821510271715916 2119 48-49 2.0181059130138443 2119 50-51 2.203977906170632 2119 52-53 -0.5658708487141482 2119 54-55 0.3212406451859593 2119 56-57 1.0996630849213709 2119 58-59 -0.09996900748861748 2119 60-61 -0.626960099035319 2119 62-63 1.7006908766270783 2119 64-65 3.123104057572519 2119 66-67 -0.05924828489176548 2119 68-69 0.44117292524585494 2119 70-71 3.4279202515174667 2119 72-73 0.45134705865460134 2119 74-75 -2.09412648933203 2119 76 -1.1703068924739242 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 1.0 12 52.0 13 260.0 14 572.0 15 827.0 16 1063.0 17 1266.0 18 1319.0 19 1336.0 20 1100.0 21 867.0 22 669.0 23 421.0 24 306.0 25 192.0 26 167.0 27 103.0 28 68.0 29 57.0 30 50.0 31 35.0 32 34.0 33 19.0 34 20.0 35 27.0 36 12.0 37 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.976313841598817 27.609178386380457 28.155070318282753 37.259437453737974 2 17.26612087204489 24.726336123631683 32.96844816484224 25.03909483948119 3 5.261705454879956 28.709410357832766 39.2236224818324 26.805261705454882 4 4.047465734523043 26.906448348817957 23.797258761843437 45.248827154815565 5 3.0907920154539603 30.742342010854568 21.313586606567934 44.85327936712354 6 19.1610707386625 30.567565081409253 12.556342562781714 37.71502161714654 7 5.5100726704075065 34.73461503081593 10.587802410081869 49.167509888694696 8 13.742985925857788 35.07497010394628 13.95455799834422 37.22748597185171 9 13.007083065035415 27.945911139729557 11.056940483856131 47.9900653113789 10-11 8.403090792015453 30.406586330604362 23.479900653113788 37.7104222242664 12-13 8.099530861926226 27.320393708030537 23.332720080949315 41.247355349093915 14-15 12.432158955017938 31.050501333823938 18.392972127679148 38.12436758347898 16-17 16.281850795694968 30.751540796614847 17.864041946463065 35.10256646122712 18-19 11.930825131082697 28.718609143593042 17.399503265568946 41.95106245975531 20-21 10.647594517523688 29.463710790175696 18.1216079477509 41.76708674454972 22-23 17.358108729647686 29.201545396007727 15.076809861098333 38.363536013246254 24-25 13.007083065035415 35.45671971299789 14.317910035875265 37.21828718609144 26-27 10.960353233373194 35.15315978290865 19.230061631864594 34.65642535185356 28-29 13.807377426179745 30.85732683285806 22.47263361236317 32.86266212859903 30-31 13.384233281206882 30.884923190138903 23.69147272560022 32.039370803054 32-33 10.394627909115997 36.37659828902585 20.87664428295465 32.3521295189035 34-35 10.05427283598565 34.68402170913439 20.145340815012418 35.116364639867534 36-37 11.158127127219206 30.259405758439883 23.318921902308894 35.263545212032014 38-39 11.751448808757244 26.23953638119768 26.538496918406768 35.4705178916383 40-41 9.152791831478245 28.56682917854843 24.1284150492135 38.15196394075982 42-43 5.583662956489743 26.846656241376138 27.821727531965777 39.74795327016834 44-45 7.262441357740778 28.732407322233467 28.60362432158955 35.4015269984362 46-47 7.598197037990985 32.33833134026309 23.953638119768193 36.10983350197774 48-49 8.196118112409161 34.578235672891175 19.66240456259774 37.56324165210192 50-51 7.933952718241192 32.766074878116086 18.535553306963482 40.76441909667924 52-53 6.489743353877288 31.758807837365467 20.706466746389477 41.044982062367765 54-55 6.986477784932389 31.271272192070647 19.234661024744735 42.50758899825223 56-57 10.767178732407322 31.69901572992365 17.657069266856777 39.87673627081225 58-59 9.120596081317265 30.475577223806454 19.188667095943334 41.21515959893294 60-61 6.885291141569313 28.925581823199337 19.266856774905712 44.92227026032564 62-63 8.73424707938552 31.10569404838561 17.693864409897895 42.466194462330975 64-65 9.125195474197406 33.01904148652378 14.630668751724773 43.22509428755404 66-67 7.221046821819519 37.443657437218285 13.531413853371355 41.80388188759084 68-69 9.281574832122159 37.48505197313954 13.140465458559468 40.09290773617882 70-71 8.311102934412657 38.46012326372919 13.361236316806183 39.867537485051976 72-73 8.338699291693496 38.22555422684206 11.981418452764235 41.45432802870021 74-75 9.410357832766076 37.94039186827339 12.082605096127311 40.56664520283322 76 7.561401894949866 39.38920062551743 12.409161990617239 40.64023548891546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 2.0 14 3.0 15 3.0 16 3.5 17 4.0 18 4.5 19 7.5 20 9.0 21 8.0 22 11.0 23 19.0 24 25.5 25 27.0 26 30.0 27 40.0 28 48.5 29 50.0 30 53.0 31 66.0 32 96.0 33 116.0 34 131.0 35 175.0 36 225.5 37 247.0 38 253.0 39 308.5 40 395.0 41 463.0 42 494.0 43 537.5 44 622.0 45 679.0 46 695.0 47 705.0 48 709.5 49 675.0 50 646.0 51 643.5 52 604.0 53 536.0 54 505.0 55 487.5 56 441.5 57 361.0 58 309.0 59 303.0 60 251.5 61 196.5 62 187.0 63 171.0 64 133.0 65 98.5 66 80.5 67 75.0 68 65.5 69 48.0 70 29.5 71 19.0 72 19.0 73 19.5 74 15.0 75 10.0 76 10.5 77 7.0 78 4.5 79 6.0 80 5.5 81 3.5 82 2.5 83 3.0 84 3.0 85 2.5 86 2.0 87 2.0 88 3.0 89 3.5 90 2.0 91 1.5 92 2.0 93 1.5 94 1.0 95 1.5 96 2.0 97 2.0 98 2.5 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5795235028976174 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 10871.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.48486799742435 #Duplication Level Percentage of deduplicated Percentage of total 1 99.73185390661119 99.21810321037623 2 0.2034211742949607 0.4047465734523043 3 0.027739251040221912 0.08278907184251678 4 0.009246417013407305 0.036795143041118576 5 0.009246417013407305 0.045993928801398215 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.009246417013407305 0.08278907184251678 >10 0.009246417013407305 0.128783000643915 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC 14 0.128783000643915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009198785760279642 0.0 2 0.0 0.0 0.0 0.009198785760279642 0.0 3 0.0 0.0 0.0 0.009198785760279642 0.0 4 0.0 0.0 0.0 0.009198785760279642 0.0 5 0.0 0.0 0.0 0.009198785760279642 0.0 6 0.0 0.0 0.0 0.009198785760279642 0.0 7 0.0 0.0 0.0 0.009198785760279642 0.0 8 0.0 0.0 0.0 0.018397571520559285 0.0 9 0.0 0.0 0.0 0.018397571520559285 0.0 10 0.0 0.0 0.0 0.018397571520559285 0.0 11 0.0 0.0 0.0 0.02759635728083893 0.0 12 0.0 0.0 0.0 0.02759635728083893 0.0 13 0.0 0.0 0.0 0.02759635728083893 0.0 14 0.0 0.0 0.0 0.02759635728083893 0.0 15 0.0 0.0 0.0 0.02759635728083893 0.0 16 0.0 0.0 0.0 0.02759635728083893 0.0 17 0.0 0.0 0.0 0.02759635728083893 0.0 18 0.0 0.0 0.0 0.02759635728083893 0.0 19 0.0 0.0 0.0 0.03679514304111857 0.0 20 0.0 0.0 0.0 0.03679514304111857 0.0 21 0.0 0.0 0.0 0.045993928801398215 0.0 22 0.0 0.0 0.0 0.045993928801398215 0.0 23 0.0 0.0 0.0 0.045993928801398215 0.0 24 0.0 0.0 0.0 0.045993928801398215 0.0 25 0.0 0.0 0.0 0.045993928801398215 0.0 26 0.0 0.0 0.0 0.045993928801398215 0.0 27 0.0 0.0 0.0 0.045993928801398215 0.0 28 0.0 0.0 0.0 0.045993928801398215 0.0 29 0.0 0.0 0.0 0.045993928801398215 0.0 30 0.0 0.0 0.0 0.045993928801398215 0.0 31 0.0 0.0 0.0 0.045993928801398215 0.0 32 0.0 0.0 0.0 0.05519271456167786 0.0 33 0.0 0.0 0.0 0.0643915003219575 0.0 34 0.0 0.0 0.0 0.09198785760279643 0.0 35 0.0 0.0 0.0 0.11038542912335572 0.0 36 0.0 0.0 0.0 0.14718057216447428 0.0 37 0.0 0.0 0.0 0.17477692944531323 0.0 38 0.0 0.0 0.0 0.24836721552755037 0.0 39 0.0 0.0 0.0 0.29436114432894855 0.0 40 0.0 0.0 0.0 0.33115628737006714 0.0 41 0.0 0.0 0.0 0.35875264465090606 0.0 42 0.0 0.0 0.0 0.42314414497286357 0.0 43 0.0 0.0 0.0 0.45993928801398215 0.0 44 0.0 0.0 0.0 0.4875356452948211 0.0 45 0.0 0.0 0.0 0.5335295740962193 0.0 46 0.0 0.0 0.0 0.5887222886578971 0.0 47 0.0 0.0 0.0 0.6347162174592954 0.0 48 0.0 0.0 0.0 0.6899089320209733 0.0 49 0.0 0.0 0.0 0.7267040750620918 0.0 50 0.0 0.0 0.0 0.7451016465826511 0.0 51 0.0 0.0 0.0 0.7451016465826511 0.0 52 0.0 0.0 0.0 0.77269800386349 0.0 53 0.0 0.0 0.0 0.7818967896237696 0.0 54 0.0 0.0 0.0 0.7818967896237696 0.0 55 0.0 0.0 0.0 0.8094931469046086 0.0 56 0.0 0.0 0.0 0.8370895041854475 0.0 57 0.0 0.0 0.0 0.8738846472265661 0.0 58 0.0 0.0 0.0 0.9106797902676846 0.0 59 0.0 0.0 0.0 0.9566737190690828 0.0 60 0.0 0.0 0.0 1.002667647870481 0.0 61 0.0 0.0 0.0 1.0210652193910403 0.0 62 0.0 0.0 0.0 1.0210652193910403 0.0 63 0.0 0.0 0.0 1.057860362432159 0.0 64 0.0 0.0 0.0 1.0670591481924385 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACATC 165 5.8176817E-4 14.847507 7 CACATCT 155 0.006777326 13.547496 8 >>END_MODULE