FastQCFastQC Report
Wed 25 May 2016
SRR1780372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10871
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT279825.73820255726244No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT209219.24385981050501RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC6455.933216815380369No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG3623.3299604452212304No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC3172.916015086008647No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT1881.729371722932573No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT1621.4902032931653022No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC960.8830834329868458No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT940.8646858614662865No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG890.8186919326648883No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC860.7910955753840493No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT810.7451016465826511No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT700.643915003219575No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT680.6255174316990157No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC530.4875356452948211No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC510.4691380737742618No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC490.4507405022537025No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT470.4323429307331432No Hit
CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC440.4047465734523043No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG440.4047465734523043No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT320.2943611443289486No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT280.25756600128783No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT260.2391684297672707No Hit
CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT260.2391684297672707No Hit
ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT260.2391684297672707RNA PCR Primer, Index 15 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG260.2391684297672707No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG260.2391684297672707No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG250.22996964400699105No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCACGTATGCCGT250.22996964400699105No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT210.19317450096587252Illumina PCR Primer Index 4 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT210.19317450096587252No Hit
TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT200.18397571520559286No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG200.18397571520559286No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT200.18397571520559286TruSeq Adapter, Index 19 (95% over 21bp)
CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT190.17477692944531323No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT180.16557814368503357Illumina PCR Primer Index 7 (95% over 21bp)
ATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT180.16557814368503357RNA PCR Primer, Index 36 (95% over 21bp)
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG180.16557814368503357No Hit
CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT180.16557814368503357No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCCT170.15637935792475394No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGT160.1471805721644743No Hit
CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT160.1471805721644743No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC150.13798178640419465No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC150.13798178640419465No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT150.13798178640419465No Hit
CTTATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT140.128783000643915No Hit
ATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTT140.128783000643915RNA PCR Primer, Index 36 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAGCTCGTATGCCGT140.128783000643915No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT130.11958421488363535No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCCCGTATGCCGT130.11958421488363535No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCGGACAATCTCGTATGCCGT130.11958421488363535No Hit
ATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGTCTT130.11958421488363535RNA PCR Primer, Index 36 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGAACGCGGACAATCTCGTATGCCGTCTT130.11958421488363535RNA PCR Primer, Index 36 (95% over 21bp)
CTTATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGT130.11958421488363535No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT130.11958421488363535No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGGACAATCTCGTATGCCGT130.11958421488363535No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGTCTT120.11038542912335572RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATTTCGTATGCCGT120.11038542912335572No Hit
ATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGTCTT120.11038542912335572RNA PCR Primer, Index 36 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT120.11038542912335572RNA PCR Primer, Index 36 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC120.11038542912335572No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACATCTCGTATGCCGTC120.11038542912335572No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGTC110.10118664336307608No Hit
CTTATACACATCTCCGAGCCCACGAGAACGCGGACAATCTCGTATGCCGT110.10118664336307608No Hit
CTCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG110.10118664336307608No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGC110.10118664336307608No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC110.10118664336307608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT252.6558282E-670.000011
TGGTTGC150.002165817470.021
AACGCGG150.002165817470.027
GAGAACG150.002165817470.024
GGTTGCG150.002165817470.022
TAAATGG150.002165817470.017
ATGGTTG150.002165817470.020
GAACGCG150.002165817470.026
AGAACGC150.002165817470.025
ACGAGAA150.002165817470.022
AATGGTT150.002165817470.019
TTGCGGC150.002165817470.024
CGAGAAC150.002165817470.023
ACGCGGA150.002165817470.028
AAATGGT150.002165817470.018
TGCGGCT150.002165817470.025
GTTGCGG150.002165817470.023
CTTTACA900.069.999991
TTTACAC900.069.999992
TTACACA950.062.6315773
Produced by FastQC (version 0.11.5)