##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780372_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10871 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74822923374115 34.0 34.0 34.0 31.0 34.0 2 33.33088032379726 34.0 34.0 34.0 33.0 34.0 3 33.46030723944439 34.0 34.0 34.0 34.0 34.0 4 33.517891638303745 34.0 34.0 34.0 34.0 34.0 5 33.46803421948303 34.0 34.0 34.0 34.0 34.0 6 37.301904148652376 38.0 37.0 38.0 37.0 38.0 7 36.82697083984914 38.0 37.0 38.0 35.0 38.0 8 36.89908932020973 38.0 37.0 38.0 35.0 38.0 9 36.4505565265385 38.0 37.0 38.0 34.0 38.0 10-11 36.22201269432435 38.0 37.0 38.0 33.0 38.0 12-13 35.83860730383589 38.0 36.5 38.0 31.5 38.0 14-15 35.84426455707847 38.0 36.0 38.0 31.0 38.0 16-17 35.72330052433079 38.0 36.0 38.0 31.0 38.0 18-19 35.7927053628921 38.0 36.0 38.0 31.0 38.0 20-21 35.70665072210468 38.0 36.0 38.0 31.0 38.0 22-23 35.71833318002024 38.0 36.0 38.0 32.5 38.0 24-25 34.63678594425536 37.0 34.0 38.0 28.0 38.0 26-27 34.756416153067796 37.0 34.0 38.0 29.0 38.0 28-29 34.60629196946003 37.0 34.5 38.0 28.0 38.0 30-31 33.685861466286454 37.0 33.5 38.0 23.0 38.0 32-33 34.42456995676571 37.0 34.0 38.0 25.0 38.0 34-35 33.26083157023273 37.0 32.5 38.0 23.0 38.0 36-37 33.245147640511455 37.0 34.0 38.0 21.5 38.0 38-39 30.677398583386992 34.5 26.5 37.0 11.0 38.0 40-41 32.762625333455986 36.5 32.5 37.0 18.0 38.0 42-43 34.52672247263361 37.0 34.0 38.0 26.5 38.0 44-45 35.01343022721001 37.0 35.0 38.0 29.0 38.0 46-47 34.90060711986018 37.0 35.0 38.0 27.0 38.0 48-49 34.4604452212308 37.0 34.5 38.0 25.5 38.0 50-51 34.216585410725784 37.0 34.0 38.0 24.5 38.0 52-53 33.81078097691105 37.0 34.0 38.0 24.0 38.0 54-55 34.30401986937724 37.0 34.0 38.0 25.5 38.0 56-57 34.24556158587067 37.0 34.0 38.0 24.0 38.0 58-59 33.976129150952076 37.0 34.0 38.0 24.0 38.0 60-61 34.01605188115169 37.0 34.0 38.0 24.0 38.0 62-63 34.83906724312391 37.0 34.5 38.0 27.0 38.0 64-65 34.837871400975075 37.0 35.0 38.0 28.0 38.0 66-67 32.44480728543832 36.5 31.0 38.0 21.0 38.0 68-69 27.724128415049215 32.5 20.5 37.0 11.0 38.0 70-71 23.655137521847116 25.0 11.0 33.5 10.0 37.0 72-73 19.66980958513476 19.5 11.0 26.0 10.0 33.5 74-75 18.930687149296293 19.0 11.0 25.0 10.0 33.0 76 19.492686965320576 19.0 11.0 25.0 10.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.018634425195792 1101 2 0.43134495798810946 1101 3 0.30420047606035894 1101 4 0.3725830131648067 1101 5 0.5560147403321096 1101 6 -0.5688714519261424 1101 7 0.5703527045056589 1101 8 0.7309575019613916 1101 9 1.4089485834066977 1101 10-11 0.35794612819397287 1101 12-13 1.4579583143054364 1101 14-15 1.561173630471373 1101 16-17 -0.3631193770786325 1101 18-19 0.5844365164483918 1101 20-21 0.3887378030246751 1101 22-23 0.6855749938579123 1101 24-25 -2.8561944152109007 1101 26-27 -0.29396483424343245 1101 28-29 1.1405670666152758 1101 30-31 2.1128145062023336 1101 32-33 1.0514268241555769 1101 34-35 2.858801380322369 1101 36-37 0.4360598436546539 1101 38-39 1.9949457440540606 1101 40-41 1.5401626694916075 1101 42-43 1.2863925521685502 1101 44-45 0.7915974051908492 1101 46-47 2.3845766353451836 1101 48-49 -1.8331496791333848 1101 50-51 -0.6362533370858685 1101 52-53 -0.8833022206581944 1101 54-55 -1.3815948142038437 1101 56-57 -2.1534296878029195 1101 58-59 0.5956725507563405 1101 60-61 -0.9392637532331776 1101 62-63 9.72028744712361E-4 1101 64-65 -0.7616433123604054 1101 66-67 -1.6512087337714938 1101 68-69 0.7289398201141744 1101 70-71 -0.908341183979406 1101 72-73 -1.1060943621140744 1101 74-75 -1.205928193090724 1101 76 1.8531820049560181 1102 1 -2.231365574804208 1102 2 -0.44365504201189054 1102 3 -0.057979011119130064 1102 4 0.2988650644468578 1102 5 0.2867839711013431 1102 6 0.2163849583302735 1102 7 0.2658655250184765 1102 8 -0.04788865188476166 1102 9 0.7679229423810554 1102 10-11 -0.07314361539577874 1102 12-13 0.8121249809721007 1102 14-15 0.17014798944573784 1102 16-17 0.4525857511264988 1102 18-19 -0.9219737399618566 1102 20-21 0.055404469691339386 1102 22-23 0.5525621733450876 1102 24-25 -6.84337390239039 1102 26-27 -3.566400731679334 1102 28-29 -0.058150882102665946 1102 30-31 2.569545275433107 1102 32-33 0.577067849796606 1102 34-35 -0.5706857991648135 1102 36-37 -4.3940683614735505 1102 38-39 -0.5899901533818408 1102 40-41 1.995290874619812 1102 42-43 1.1790207572967617 1102 44-45 1.512751251344696 1102 46-47 1.9839356097041616 1102 48-49 -4.06712403810775 1102 50-51 -0.9904200037525399 1102 52-53 -1.6974047847607565 1102 54-55 1.0799436473346162 1102 56-57 0.9443267224534893 1102 58-59 -1.1815710389872507 1102 60-61 -5.546635548104973 1102 62-63 -2.0214638686911925 1102 64-65 1.2239336107165144 1102 66-67 2.8183425482797873 1102 68-69 -1.0819576157832564 1102 70-71 -1.357059132697355 1102 72-73 -0.7903892339089467 1102 74-75 0.3725974479349148 1102 76 1.2249768767508904 1103 1 -0.2797526715784002 1103 2 0.16924818379455786 1103 3 -0.726713502434265 1103 4 -0.03736322339433684 1103 5 -0.6375336467646662 1103 6 0.22010704269751358 1103 7 0.3378258227852271 1103 8 0.7511187922839753 1103 9 0.1885184758798104 1103 10-11 -0.2623495707307626 1103 12-13 -2.324971793221451 1103 14-15 -0.5060306706038915 1103 16-17 -0.4525010975087369 1103 18-19 0.26723221537312725 1103 20-21 0.19250124388489098 1103 22-23 0.35156961751382454 1103 24-25 -4.528237425963592 1103 26-27 -0.9324056944584953 1103 28-29 -0.49921787962128406 1103 30-31 1.3648306352345898 1103 32-33 1.6508891897469766 1103 34-35 -0.31324163043032627 1103 36-37 -1.1943165004313698 1103 38-39 -1.931801567773899 1103 40-41 0.05360352970666327 1103 42-43 0.4712043801255419 1103 44-45 2.158532889061817 1103 46-47 2.192372334269919 1103 48-49 -3.197396990961348 1103 50-51 -0.264613552139636 1103 52-53 1.4365902524600855 1103 54-55 -0.5912722335586835 1103 56-57 1.2625649358529856 1103 58-59 -1.1375263739748434 1103 60-61 -5.230258376889097 1103 62-63 -0.6294043153413185 1103 64-65 0.7685986231234665 1103 66-67 -4.893816260653214 1103 68-69 -1.2885332981653939 1103 70-71 2.4680029020421017 1103 72-73 -0.02612124383450265 1103 74-75 0.16167933379099608 1103 76 -0.20058143590419775 1104 1 -0.35636557480420805 1104 2 -0.22490504201189054 1104 3 0.15836714272702324 1104 4 -0.335750320168529 1104 5 -0.6627352596678904 1104 6 0.2332118814071933 1104 7 0.7370193711723232 1104 8 0.7934575019613916 1104 9 1.450615250073362 1104 10-11 -1.3712205384726985 1104 12-13 0.7652499809721007 1104 14-15 1.0559652971380444 1104 16-17 0.3972972895880389 1104 18-19 1.3604781831150632 1104 20-21 -0.06959553030866061 1104 22-23 -0.10609167280875909 1104 24-25 -0.7676527485442364 1104 26-27 1.4768684990898961 1104 28-29 2.1822337332819473 1104 30-31 0.4096895062023336 1104 32-33 0.5097601574889126 1104 34-35 -0.34953195301096684 1104 36-37 1.4568931769879896 1104 38-39 2.718904077387389 1104 40-41 1.613079336158279 1104 42-43 0.6405592188352216 1104 44-45 1.7447224051908492 1104 46-47 1.754368302011855 1104 48-49 -0.5623163458000562 1104 50-51 1.75957999624746 1104 52-53 -0.09163555399153012 1104 54-55 0.009030185796156331 1104 56-57 0.47677864553040905 1104 58-59 -2.430369115910331 1104 60-61 1.0555279134334867 1104 62-63 0.39680536207804096 1104 64-65 0.8164816876395946 1104 66-67 0.640457932895174 1104 68-69 1.78102315344751 1104 70-71 1.1489504826872619 1104 72-73 -0.9550526954474066 1104 74-75 -3.231969859757392 1104 76 -1.6259846617106497 1105 1 0.8686344251957934 1105 2 0.5563449579881095 1105 3 0.31015285701273854 1105 4 -0.4143217487399582 1105 5 0.2417290260463929 1105 6 0.3823190242643335 1105 7 0.9120193711723203 1105 8 -0.4083282123243208 1105 9 0.8327581072162218 1105 10-11 1.1537794615273 1105 12-13 -0.2950178761707605 1105 14-15 0.7064117257094722 1105 16-17 -0.030381281840533347 1105 18-19 0.22253175454363827 1105 20-21 0.9625473268341977 1105 22-23 0.23497975576266583 1105 24-25 0.7247579657414747 1105 26-27 0.3215113562327545 1105 28-29 1.1072337332819444 1105 30-31 1.286028791916614 1105 32-33 0.649045871774625 1105 34-35 -0.47453195301096684 1105 36-37 1.0301074627022757 1105 38-39 -0.7806494940411817 1105 40-41 0.6568293361582818 1105 42-43 1.6191306474066494 1105 44-45 1.3308831194765602 1105 46-47 1.8762433020118578 1105 48-49 0.43946936848566054 1105 50-51 0.623865710533174 1105 52-53 0.2762215888656172 1105 54-55 0.8184051857961592 1105 56-57 1.4745465026732631 1105 58-59 0.44864874123252463 1105 60-61 0.7434743420049159 1105 62-63 -0.13355178077910068 1105 64-65 1.6865709733538807 1105 66-67 1.8458150757523129 1105 68-69 1.6801302963046538 1105 70-71 2.201182625544405 1105 72-73 -2.7662134097331226 1105 74-75 -0.692237716900248 1105 76 -3.2134846617106483 1106 1 -0.9099370033756351 1106 2 0.020630672273824757 1106 3 0.13158142844130793 1106 4 0.2713925369743251 1106 5 -0.4082709739536057 1106 6 0.425176167121478 1106 7 -1.2808377716848227 1106 8 0.5345289305328222 1106 9 -1.817241892783784 1106 10-11 0.23592231867016267 1106 12-13 1.5799821238292395 1106 14-15 -0.9328739885762403 1106 16-17 0.8481901467309001 1106 18-19 1.2332460402579244 1106 20-21 0.5911187554056241 1106 22-23 1.0992654700483797 1106 24-25 -0.02881346282994457 1106 26-27 0.7893684990898961 1106 28-29 -0.04990912386091395 1106 30-31 0.7646002204880489 1106 32-33 0.9026173003460514 1106 34-35 1.5968966184176026 1106 36-37 1.3229646055594202 1106 38-39 0.8729219345302468 1106 40-41 0.2782579075868483 1106 42-43 1.4977020759780828 1106 44-45 1.6130259766194186 1106 46-47 0.8548147305832856 1106 48-49 1.7501836541999438 1106 50-51 -1.2939914323239705 1106 52-53 0.10479301743703928 1106 54-55 -0.9351662427752743 1106 56-57 -0.8468820687553062 1106 58-59 1.0272201698039538 1106 60-61 0.9327600562906255 1106 62-63 1.4950196477923257 1106 64-65 0.06871383049673341 1106 66-67 2.2743865043237435 1106 68-69 2.93727315344751 1106 70-71 1.6333254826872619 1106 72-73 -1.3412134097331219 1106 74-75 -2.435094859757392 1106 76 -0.8849132331392227 1107 1 1.086816243377612 1107 2 -0.2921398904967418 1107 3 0.5958671427270232 1107 4 0.414249679831471 1107 5 0.46510564942301613 1107 6 0.5182497601950686 1107 7 1.0514133105662609 1107 8 -0.5001031040992174 1107 9 0.6665243409824555 1107 10-11 0.8143855221333638 1107 12-13 0.22879164763877213 1107 14-15 0.7505675698653178 1107 16-17 0.4683200168607655 1107 18-19 0.8723153043271878 1107 20-21 1.032677196964066 1107 22-23 -0.41480379402088374 1107 24-25 1.2044116453951546 1107 26-27 -0.33563150091010385 1107 28-29 1.5042034302516427 1107 30-31 1.9366781425659738 1107 32-33 2.513548036276788 1107 34-35 1.6466801682011578 1107 36-37 2.523181055775865 1107 38-39 0.46653665314496706 1107 40-41 0.44167782100676334 1107 42-43 2.2238925521685573 1107 44-45 1.3408398294332713 1107 46-47 1.7671523929209485 1107 48-49 2.079729108745397 1107 50-51 2.7368527235201867 1107 52-53 2.8666977793417985 1107 54-55 1.9971930645840317 1107 56-57 1.9507369788637376 1107 58-59 1.7187786113623957 1107 60-61 2.581569580100158 1107 62-63 1.2850629378356189 1107 64-65 2.3311597179426258 1107 66-67 -0.6303754004381545 1107 68-69 2.7857580019323613 1107 70-71 0.15226487662665278 1107 72-73 -1.52465496817468 1107 74-75 0.9891475644850303 1107 76 1.731969883743897 1108 1 -1.378424398333621 1108 2 -0.09071386554130356 1108 3 -0.3158975631553318 1108 4 0.20836732689029702 1108 5 -0.0028087890796584247 1108 6 -1.1252440009457487 1108 7 -0.7115100405923798 1108 8 -1.0999248509797823 1108 9 0.663850544191007 1108 10-11 0.3273088732920115 1108 12-13 1.0474190986191587 1108 14-15 -0.007453820509013553 1108 16-17 -1.729540945706077 1108 18-19 0.0011031831150631888 1108 20-21 1.2171691755736944 1108 22-23 -0.3377093198675851 1108 24-25 0.1634134279263577 1108 26-27 -0.6885726773806908 1108 28-29 -1.4868839137768788 1108 30-31 0.6385498003199785 1108 32-33 0.5759366280771445 1108 34-35 -0.2686496000697929 1108 36-37 0.8649814122821056 1108 38-39 -0.02098562849496588 1108 40-41 0.22153521851122093 1108 42-43 -0.6535584282236044 1108 44-45 0.6813032875437912 1108 46-47 1.514478596129507 1108 48-49 1.6251836541999438 1108 50-51 1.5831094080121701 1108 52-53 0.4304232695378758 1108 54-55 0.5007581269726202 1108 56-57 0.10269776317746704 1108 58-59 1.0356235311484951 1108 60-61 1.4737264428452548 1108 62-63 1.238717126783925 1108 64-65 0.46262139352194254 1108 66-67 1.732369697601058 1108 68-69 3.18727315344751 1108 70-71 -0.9181451055480352 1108 72-73 0.23336642219965142 1108 74-75 1.1531404343602532 1108 76 0.6276918088775858 1109 1 -1.606365574804208 1109 2 -0.19365504201189054 1109 3 0.5646171427270232 1109 4 0.320499679831471 1109 5 -0.006485259667890375 1109 6 -0.07928811859280671 1109 7 -0.7629806288276768 1109 8 0.13720750196139164 1109 9 1.200615250073362 1109 10-11 -0.18372053847269854 1109 12-13 0.32774998097210073 1109 14-15 0.7278402971380444 1109 16-17 0.6160472895880389 1109 18-19 0.8448531831150632 1109 20-21 1.4147794696913394 1109 22-23 0.7064083271912409 1109 24-25 -0.8770277485442364 1109 26-27 0.5237434990898961 1109 28-29 -2.0677662667180527 1109 30-31 1.1909395062023336 1109 32-33 1.6347601574889126 1109 34-35 -0.16203195301096684 1109 36-37 1.8006431769879896 1109 38-39 3.093904077387389 1109 40-41 0.4099543361582789 1109 42-43 1.7343092188352216 1109 44-45 1.0884724051908492 1109 46-47 1.348118302011855 1109 48-49 1.1720586541999438 1109 50-51 0.9939549962474601 1109 52-53 -1.1228855539915301 1109 54-55 -1.8659698142038437 1109 56-57 -0.11697135446959095 1109 58-59 0.2415058840896691 1109 60-61 -0.30384708656651327 1109 62-63 -0.728194637921959 1109 64-65 1.8946066876395946 1109 66-67 2.140457932895174 1109 68-69 0.09352315344751005 1109 70-71 0.7895754826872619 1109 72-73 2.2480723045525934 1109 74-75 2.314905140242608 1109 76 1.6865153382893503 1110 1 -0.8828807263193603 1110 2 -1.140624738981586 1110 3 -0.1314055845457034 1110 4 0.11121937680116645 1110 5 0.22268140699877392 1110 6 0.18491642686174004 1110 7 0.6877769469299011 1110 8 0.8029271989310871 1110 9 1.3028879773460886 1110 10-11 -1.0644023566545187 1110 12-13 0.6984886173357339 1110 14-15 1.3717796910774354 1110 16-17 0.8925624411031876 1110 18-19 -0.8549574229455459 1110 20-21 1.138737803024668 1110 22-23 -0.5208644000814857 1110 24-25 -1.128921687938174 1110 26-27 1.6795200142414117 1110 28-29 1.9435973696455875 1110 30-31 0.3003145062023336 1110 32-33 1.2408207635495216 1110 34-35 1.601225622746611 1110 36-37 2.704999237594052 1110 38-39 -0.2607360741277631 1110 40-41 -3.088625209296268 1110 42-43 -0.018531690255684907 1110 44-45 1.5378095264029739 1110 46-47 1.8277584535270108 1110 48-49 2.807001836018131 1110 50-51 -0.46011697344950875 1110 52-53 1.2909402035842277 1110 54-55 -0.442200874809906 1110 56-57 1.0416460697728311 1110 58-59 -1.3872819946982133 1110 60-61 -0.9790364805059042 1110 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-3.9822272522900093 2116 68-69 -4.007171290996933 2116 70-71 -2.5750078506460703 2116 72-73 -0.15933510285481134 2116 74-75 -0.21287263753517038 2116 76 -3.887558735784724 2117 1 0.9353010918624562 2117 2 0.014678291321445158 2117 3 0.5958671427270232 2117 4 0.3725830131648067 2117 5 -0.027318593001226077 2117 6 0.3894618814071933 2117 7 0.5286860378389875 2117 8 -0.18570916470527266 2117 9 -0.007718083259973696 2117 10-11 -1.97538720513937 2117 12-13 -2.771208352361228 2117 14-15 -0.29299303619529127 2117 16-17 -2.7381193770786325 2117 18-19 -3.061396816884937 2117 20-21 -0.361262196975332 2117 22-23 0.14390832719124091 2117 24-25 -3.5020277485442364 2117 26-27 -2.189798167576768 2117 28-29 -1.359432933384717 2117 30-31 -1.2830188271310021 2117 32-33 -2.448573175844423 2117 34-35 0.9629680469890332 2117 36-37 -1.8347734896786783 2117 38-39 -2.296720922612611 2117 40-41 -1.4598373305083854 2117 42-43 -0.2136074478314427 2117 44-45 -2.9792359281424865 2117 46-47 -2.4695900313214736 2117 48-49 -1.3748163458000562 2117 50-51 -2.92792000375254 2117 52-53 1.0541977793417985 2117 54-55 -0.46492814753717226 2117 56-57 1.742403645530409 2117 58-59 -2.5293274492436666 2117 60-61 -2.6059304198998454 2117 62-63 -1.1240279712552876 2117 64-65 -1.5741433123604054 2117 66-67 -0.5678754004381616 2117 68-69 -2.68772684655249 2117 70-71 -1.9708411839794024 2117 72-73 -1.4810943621140709 2117 74-75 -0.18509485975739182 2117 76 -1.4384846617106497 2118 1 -1.302794146232781 2118 2 -0.15794075629760584 2118 3 -1.618418571558692 2118 4 0.3071068226886169 2118 5 0.3060147403321096 2118 6 0.425176167121478 2118 7 0.8977336568866079 2118 8 0.49881464481853754 2118 9 -2.281527607069492 2118 10-11 -0.06764910990126793 2118 12-13 -0.6343035904564687 2118 14-15 0.40641172570947504 2118 16-17 -3.919666996126246 2118 18-19 -1.7667539597420756 2118 20-21 -1.6767383874515218 2118 22-23 0.2599797557626715 2118 24-25 1.1676151085986248 2118 26-27 -3.8713457866243886 2118 28-29 -1.8534805524323374 2118 30-31 -5.021114065226236 2118 32-33 -1.8652398425110874 2118 34-35 -0.43881766729668215 2118 36-37 -6.069892537297726 2118 38-39 -2.323506636898326 2118 40-41 0.9568293361582789 2118 42-43 1.2834163616923604 2118 44-45 -1.190545451952012 2118 46-47 0.2476718734404244 2118 48-49 -0.9105306315143409 2118 50-51 -3.579705718038255 2118 52-53 -4.59163555399153 2118 54-55 -0.7923090999181355 2118 56-57 0.08168935981612435 2118 58-59 -2.0442084016246156 2118 60-61 -2.763668515137944 2118 62-63 -2.790694637921959 2118 64-65 -1.0205718837889748 2118 66-67 0.6851007900380353 2118 68-69 -2.134155417981063 2118 70-71 -1.5631030887413075 2118 72-73 1.355215161695451 2118 74-75 0.7613337116711776 2118 76 0.5079439097179232 2119 1 0.893634425195792 2119 2 -0.06865504201189054 2119 3 -0.23746619060631247 2119 4 -0.7524169868351933 2119 5 -1.1106519263345547 2119 6 -2.6105381185928067 2119 7 -0.3879806288276768 2119 8 -0.43570916470527266 2119 9 -1.799384749926638 2119 10-11 -0.9128872051393628 2119 12-13 -1.4587083523612279 2119 14-15 -0.8346597028619556 2119 16-17 -0.5714527104119611 2119 18-19 -0.6030634835516011 2119 20-21 -1.5904288636419963 2119 22-23 -1.1894250061420877 2119 24-25 -0.10619441521090778 2119 26-27 0.16436849908989615 2119 28-29 1.3697337332819473 2119 30-31 0.44614783953566217 2119 32-33 1.4680934908222412 2119 34-35 0.5254680469890332 2119 36-37 -1.563940156345346 2119 38-39 -2.734220922612611 2119 40-41 -1.418170663841721 2119 42-43 -2.067774114498107 2119 44-45 -0.8542359281424794 2119 46-47 -0.8654233646548164 2119 48-49 -1.0414830124667276 2119 50-51 0.2595799962474601 2119 52-53 -1.5499688873248658 2119 54-55 0.16007185246282774 2119 56-57 -2.4242630211362552 2119 58-59 -0.5293274492436666 2119 60-61 -2.189263753233181 2119 62-63 -1.3948613045886304 2119 64-65 -1.3241433123604054 2119 66-67 -0.38037540043816165 2119 68-69 -0.39606017988582565 2119 70-71 1.1333254826872619 2119 72-73 3.518905637885929 2119 74-75 -0.35176152642405967 2119 76 0.7698486716226824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 13.0 23 10.0 24 32.0 25 54.0 26 111.0 27 196.0 28 351.0 29 493.0 30 757.0 31 967.0 32 1409.0 33 1896.0 34 2381.0 35 1785.0 36 352.0 37 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.0762579339527183 29.316530218011223 11.719253058596266 57.8879587894398 2 0.49673443105510073 3.256370159138994 86.6709594333548 9.575935976451108 3 0.5243307883359397 33.61236316806181 62.57933952718241 3.2839665164198326 4 0.4139453592125839 45.48799558458283 24.2204029068163 29.877656149388283 5 0.5059332168153804 51.09925489835342 44.68770122343851 3.7071106613926963 6 0.5795235028976174 47.54852359488548 8.656057400423144 43.21589550179376 7 1.1038542912335572 51.50400147180573 4.801766166865973 42.59037807009475 8 0.6347162174592954 46.74822923374114 29.150952074326185 23.46610247447337 9 0.8278907184251678 23.1809401159047 3.477141017385705 72.51402814828442 10-11 0.7543004323429308 26.685677490571248 42.636371998896145 29.923650078189677 12-13 14.920430503173582 3.458743445865146 33.451384417256925 48.16944163370435 14-15 23.19013890166498 16.332444117376507 6.931285070370712 53.5461319105878 16-17 32.64189127035231 27.66534817404103 1.0440621837917394 38.64869837181492 18-19 26.409713917762858 21.313586606567934 0.7037071106613927 51.57299236500782 20-21 17.0821451568393 10.04507405022537 0.7864961825039094 72.08628461043143 22-23 23.387912795510992 39.954925949774626 0.6623125747401343 35.99484867997425 24-25 32.853463342838744 44.66010486615767 0.9428755404286634 21.543556250574923 26-27 40.21249195106246 33.91592309815105 0.9060803973875449 24.96550455339895 28-29 25.365651733971116 8.168521755128323 1.4350105786036242 65.03081593229693 30-31 47.53932480912519 15.21019225462239 1.2694324349185908 35.981050501333826 32-33 52.53886486983718 23.429307331432252 1.5453960077269802 22.48643179100359 34-35 9.336767546683838 48.095851347622116 14.313310642995125 28.25407046269892 36-37 1.3246251494802685 53.25637015913899 22.868181400055192 22.550823291325546 38-39 14.856039002851624 11.907828166681998 30.880323797258765 42.35580903320761 40-41 9.382761475485237 16.111673259129795 41.08177720540888 33.42378805997608 42-43 37.94959065403366 9.414957225646214 44.8946739030448 7.740778217275319 44-45 11.457087664428295 24.316990157299237 33.97571520559286 30.250206972679607 46-47 39.38000183975715 3.067795051053261 21.267592677766533 36.284610431423054 48-49 29.330328396651637 1.3752184711618067 23.714469690000918 45.579983442185636 50-51 6.825499034127495 1.499402078925582 48.10964952626253 43.56544936068439 52-53 14.584674822923374 1.5913899365283781 67.81344862478153 16.01048661576672 54-55 9.189586974519363 1.5453960077269802 49.49866617606476 39.7663508416889 56-57 37.889798546591855 1.9225462238984452 30.530769938368135 29.65688529114157 58-59 11.125931377058228 30.65035415325177 50.44614110937356 7.777573360316438 60-61 24.054824763131265 64.83764143133106 9.102198509796706 2.005335295740962 62-63 2.6124551559194185 94.46233097231166 0.9566737190690828 1.9685401526998436 64-65 0.49673443105510073 96.3388832674087 0.7588998252230705 2.4054824763131264 66-67 0.5611259313770582 93.98399411277711 1.1176524698739765 4.337227485971852 68-69 1.1958421488363535 84.17808849231902 2.810229049765431 11.815840309079203 70-71 2.57106061999816 74.75393248091252 5.606659920890443 17.06834697819888 72-73 3.84969184067703 61.59966884371263 9.088400331156288 25.46223898445405 74-75 4.539600772698004 59.36436390396468 9.115996688437127 26.980038634900193 76 3.458743445865146 61.668659736914734 8.21451568392972 26.658081133290406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 1.0 27 1.5 28 2.0 29 3.0 30 3.0 31 2.0 32 2.5 33 4.0 34 2.5 35 3.5 36 8.0 37 10.0 38 17.0 39 24.5 40 62.0 41 251.0 42 403.0 43 1038.5 44 2126.5 45 2554.0 46 2529.0 47 2125.0 48 1321.5 49 685.0 50 448.0 51 320.5 52 141.0 53 68.5 54 48.0 55 36.5 56 18.5 57 11.5 58 11.0 59 13.0 60 10.5 61 7.0 62 8.0 63 4.0 64 2.0 65 4.0 66 2.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 10871.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.268788519915372 #Duplication Level Percentage of deduplicated Percentage of total 1 80.96676737160121 14.791647502529667 2 8.358509566968781 3.0539968724128412 3 2.6183282980866065 1.4350105786036242 4 1.863041289023162 1.3614202925213872 5 0.6545820745216516 0.5979210744181768 6 0.5538771399798591 0.6071198601784564 7 0.6545820745216516 0.8370895041854475 8 0.25176233635448136 0.3679514304111857 9 0.5538771399798591 0.9106797902676846 >10 2.719033232628399 9.327568760923558 >50 0.4531722054380665 6.328764603072394 >100 0.2014098690835851 9.465550547327753 >500 0.050352467270896276 5.933216815380369 >1k 0.10070493454179255 44.982062367767455 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 2798 25.73820255726244 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 2092 19.24385981050501 RNA PCR Primer, Index 15 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 645 5.933216815380369 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 362 3.3299604452212304 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 317 2.916015086008647 No Hit TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 188 1.729371722932573 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT 162 1.4902032931653022 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 96 0.8830834329868458 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT 94 0.8646858614662865 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 89 0.8186919326648883 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 86 0.7910955753840493 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT 81 0.7451016465826511 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT 70 0.643915003219575 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT 68 0.6255174316990157 No Hit CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 53 0.4875356452948211 No Hit TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 51 0.4691380737742618 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 49 0.4507405022537025 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT 47 0.4323429307331432 No Hit CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 44 0.4047465734523043 No Hit CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 44 0.4047465734523043 No Hit CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT 32 0.2943611443289486 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT 28 0.25756600128783 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT 26 0.2391684297672707 No Hit CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT 26 0.2391684297672707 No Hit ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT 26 0.2391684297672707 RNA PCR Primer, Index 15 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG 26 0.2391684297672707 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG 26 0.2391684297672707 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 25 0.22996964400699105 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCACGTATGCCGT 25 0.22996964400699105 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT 21 0.19317450096587252 Illumina PCR Primer Index 4 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT 21 0.19317450096587252 No Hit TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 20 0.18397571520559286 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG 20 0.18397571520559286 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT 20 0.18397571520559286 TruSeq Adapter, Index 19 (95% over 21bp) CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 19 0.17477692944531323 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT 18 0.16557814368503357 Illumina PCR Primer Index 7 (95% over 21bp) ATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 18 0.16557814368503357 RNA PCR Primer, Index 36 (95% over 21bp) CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 18 0.16557814368503357 No Hit CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 18 0.16557814368503357 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCCT 17 0.15637935792475394 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGT 16 0.1471805721644743 No Hit CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT 16 0.1471805721644743 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC 15 0.13798178640419465 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC 15 0.13798178640419465 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT 15 0.13798178640419465 No Hit CTTATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 14 0.128783000643915 No Hit ATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTT 14 0.128783000643915 RNA PCR Primer, Index 36 (96% over 27bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAGCTCGTATGCCGT 14 0.128783000643915 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT 13 0.11958421488363535 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCCCGTATGCCGT 13 0.11958421488363535 No Hit CTTATACACATCTCCGCGCCCACGAGACCGCGGACAATCTCGTATGCCGT 13 0.11958421488363535 No Hit ATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGTCTT 13 0.11958421488363535 RNA PCR Primer, Index 36 (95% over 21bp) ATACACATCTCCGAGCCCACGAGAACGCGGACAATCTCGTATGCCGTCTT 13 0.11958421488363535 RNA PCR Primer, Index 36 (95% over 21bp) CTTATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGT 13 0.11958421488363535 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT 13 0.11958421488363535 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGGACAATCTCGTATGCCGT 13 0.11958421488363535 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGACAATCTCGTATGCCGTCTT 12 0.11038542912335572 RNA PCR Primer, Index 19 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATTTCGTATGCCGT 12 0.11038542912335572 No Hit ATACACATCTCCGAGCCCAAGAGACCGCGGACAATCTCGTATGCCGTCTT 12 0.11038542912335572 RNA PCR Primer, Index 36 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGTCTT 12 0.11038542912335572 RNA PCR Primer, Index 36 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC 12 0.11038542912335572 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACATCTCGTATGCCGTC 12 0.11038542912335572 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGTC 11 0.10118664336307608 No Hit CTTATACACATCTCCGAGCCCACGAGAACGCGGACAATCTCGTATGCCGT 11 0.10118664336307608 No Hit CTCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 11 0.10118664336307608 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGC 11 0.10118664336307608 No Hit CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC 11 0.10118664336307608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009198785760279642 0.0 2 0.0 0.0 0.0 0.009198785760279642 0.0 3 0.0 0.0 0.0 0.009198785760279642 0.0 4 0.0 0.0 0.0 0.009198785760279642 0.0 5 0.0 0.0 0.0 0.009198785760279642 0.0 6 0.0 0.0 0.0 0.009198785760279642 0.0 7 0.0 0.0 0.0 0.009198785760279642 0.0 8 0.0 0.0 0.0 0.018397571520559285 0.0 9 0.0 0.0 0.0 0.018397571520559285 0.0 10 0.0 0.0 0.0 0.018397571520559285 0.0 11 0.0 0.0 0.0 0.02759635728083893 0.0 12 0.0 0.0 0.0 0.02759635728083893 0.0 13 0.0 0.0 0.0 0.02759635728083893 0.0 14 0.0 0.0 0.0 0.02759635728083893 0.0 15 0.0 0.0 0.0 0.03679514304111857 0.0 16 0.0 0.0 0.0 0.03679514304111857 0.0 17 0.0 0.0 0.0 0.03679514304111857 0.0 18 0.0 0.0 0.0 0.03679514304111857 0.0 19 0.0 0.0 0.0 0.045993928801398215 0.0 20 0.0 0.0 0.0 0.045993928801398215 0.0 21 0.0 0.0 0.0 0.05519271456167786 0.0 22 0.0 0.0 0.0 0.05519271456167786 0.0 23 0.0 0.0 0.0 0.05519271456167786 0.0 24 0.0 0.0 0.0 0.05519271456167786 0.0 25 0.0 0.0 0.0 0.05519271456167786 0.0 26 0.0 0.0 0.0 0.05519271456167786 0.0 27 0.0 0.0 0.0 0.05519271456167786 0.0 28 0.0 0.0 0.0 0.05519271456167786 0.0 29 0.0 0.0 0.0 0.05519271456167786 0.0 30 0.0 0.0 0.0 0.05519271456167786 0.0 31 0.0 0.0 0.0 0.0643915003219575 0.0 32 0.0 0.0 0.0 0.0643915003219575 0.0 33 0.0 0.0 0.0 0.07359028608223714 0.0 34 0.0 0.0 0.0 0.07359028608223714 0.0 35 0.0 0.0 0.0 0.10118664336307608 0.0 36 0.0 0.0 0.0 0.128783000643915 0.0 37 0.0 0.0 0.0 0.16557814368503357 0.0 38 0.0 0.0 0.0 0.18397571520559286 0.0 39 0.0 0.0 0.0 0.22077085824671144 0.0 40 0.0 0.0 0.0 0.26676478704810963 0.0 41 0.0 0.0 0.0 0.2759635728083893 0.0 42 0.0 0.0 0.0 0.3127587158495079 0.0 43 0.0 0.0 0.0 0.3403550731303468 0.0 44 0.0 0.0 0.0 0.35875264465090606 0.0 45 0.0 0.0 0.0 0.35875264465090606 0.0 46 0.0 0.0 0.0 0.39554778769202464 0.0 47 0.0 0.0 0.0 0.4415417164934229 0.0 48 0.0 0.0 0.0 0.45993928801398215 0.0 49 0.0 0.0 0.0 0.4783368595345414 0.0 50 0.0 0.0 0.0 0.4875356452948211 0.0 51 0.0 0.0 0.0 0.5059332168153804 0.0 52 0.0 0.0 0.0 0.5243307883359396 0.0 53 0.0 0.0 0.0 0.5243307883359396 0.0 54 0.0 0.0 0.0 0.5243307883359396 0.0 55 0.0 0.0 0.0 0.5427283598564989 0.0 56 0.0 0.0 0.0 0.5611259313770582 0.0 57 0.0 0.0 0.0 0.5795235028976176 0.0 58 0.0 0.0 0.0 0.6255174316990157 0.0 59 0.0 0.0 0.0 0.6623125747401343 0.0 60 0.0 0.0 0.0 0.7083065035415326 0.0 61 0.0 0.0 0.0 0.7175052893018121 0.0 62 0.0 0.0 0.0 0.7175052893018121 0.0 63 0.0 0.0 0.0 0.77269800386349 0.0 64 0.0 0.0 0.0 0.77269800386349 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 25 2.6558282E-6 70.00001 1 TGGTTGC 15 0.0021658174 70.0 21 AACGCGG 15 0.0021658174 70.0 27 GAGAACG 15 0.0021658174 70.0 24 GGTTGCG 15 0.0021658174 70.0 22 TAAATGG 15 0.0021658174 70.0 17 ATGGTTG 15 0.0021658174 70.0 20 GAACGCG 15 0.0021658174 70.0 26 AGAACGC 15 0.0021658174 70.0 25 ACGAGAA 15 0.0021658174 70.0 22 AATGGTT 15 0.0021658174 70.0 19 TTGCGGC 15 0.0021658174 70.0 24 CGAGAAC 15 0.0021658174 70.0 23 ACGCGGA 15 0.0021658174 70.0 28 AAATGGT 15 0.0021658174 70.0 18 TGCGGCT 15 0.0021658174 70.0 25 GTTGCGG 15 0.0021658174 70.0 23 CTTTACA 90 0.0 69.99999 1 TTTACAC 90 0.0 69.99999 2 TTACACA 95 0.0 62.631577 3 >>END_MODULE