FastQCFastQC Report
Wed 25 May 2016
SRR1780371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105122
Sequences flagged as poor quality0
Sequence length50
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1750.1664732406156656No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG1380.13127604117121058No Hit
CTTATACACATCTGACGCTGCCGACGAAGTATGCAGTGTAGATCTCGGTG1060.1008352200300603No Hit
TCTCTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCG1060.1008352200300603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATG207.8141765E-444.01096330
CTTTACA1350.039.1581881
TTTACAC1600.035.690862
TTTAAAT250.002362171435.1417744
AATTAGA250.002362171435.141772
CTTATAC7650.032.5358431
TTATACA8100.032.2675172
TATACAC8550.031.8536513
TTACACA1850.030.8677733
CGGTGGT1400.029.82193843
TTATAGA300.005767964829.284812
ACCGTGC300.005767964829.284818
ATAATTA454.0805404E-529.2848073
TTTATAC554.9683003E-627.953683
TCTTTAT508.202897E-526.4317741
CGAAACT1005.820766E-1126.41916825
ATATCTC508.256364E-526.40657640
TAATAAG609.552972E-625.6975571
TCGGAGT701.118091E-625.13713831
GGTGGTC1400.025.10126144
Produced by FastQC (version 0.11.5)