FastQCFastQC Report
Wed 25 May 2016
SRR1780371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105122
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT4093538.94046916915584No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT2649225.201194802229793RNA PCR Primer, Index 45 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC75467.178326135347501No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG58005.517398831833488No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC40903.890717452103271No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT23402.225985045946614No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC14561.3850573619223379No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC13871.3194193413367326No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG11011.047354502387702No Hit
CTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC7300.6944312322824908No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC7140.6792108217119157No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC7070.672551892087289No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG6170.5869370826278039No Hit
CTTATCACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC4630.4404406308860182No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3980.3786077129430566No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC3290.3129696923574513No Hit
ACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCT3280.3120184166967904RNA PCR Primer, Index 45 (95% over 23bp)
CTTATTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3140.29870055744753715No Hit
CTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT2920.2777724929129963No Hit
ATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTTCTGCTT2800.266357184985065RNA PCR Primer, Index 29 (96% over 27bp)
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT2700.2568444283784555No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT2410.22925743421928807No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTCTTC2140.20357299138144253RNA PCR Primer, Index 35 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGTCTT1980.18835258081086736No Hit
TCTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT1720.16361941363368276No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGC1510.14364262475980288No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC1500.14269134909914194RNA PCR Primer, Index 12 (95% over 21bp)
TCTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT1340.1274709385285668No Hit
CTCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG1280.12176328456460113No Hit
CTTATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC1180.11225052795799167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA254.4044915E-544.018091
CTTAACA900.044.018091
TATAACA1100.044.0180853
TTATAAC1100.044.0180852
ATAACAC1050.044.0180854
CTTACAC207.8080036E-444.0180851
TCTCATA207.8080036E-444.0180851
CTCATAC207.8080036E-444.0180852
TCTTTAC207.8080036E-444.0180851
CATACAC207.8080036E-444.0180854
TCATACA207.8080036E-444.0180853
TCTTAAT850.044.0180851
TTCTGCT254.4168955E-543.99714743
TCTGCTT254.4168955E-543.99714744
TCTTCTG254.4168955E-543.99714741
CTTCTGC254.4168955E-543.99714742
TCTTTAT1650.042.6842041
CTTTACA7350.042.2214281
TTTACAC7300.042.209122
CCGTCTT29050.042.02825544
Produced by FastQC (version 0.11.5)