##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23100 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.81 24.0 12.0 34.0 12.0 34.0 2 24.40004329004329 27.0 12.0 34.0 11.0 34.0 3 24.36965367965368 27.0 11.0 34.0 11.0 34.0 4 24.43038961038961 31.0 11.0 34.0 11.0 34.0 5 24.808398268398268 31.0 11.0 34.0 11.0 34.0 6 27.104372294372293 31.0 11.0 38.0 11.0 38.0 7 27.336320346320345 32.0 11.0 37.0 11.0 38.0 8 26.976839826839825 31.0 11.0 37.0 11.0 38.0 9 27.41891774891775 33.0 12.0 37.0 12.0 38.0 10-11 26.848376623376623 31.0 11.0 37.0 11.0 38.0 12-13 26.931125541125542 31.5 11.0 38.0 11.0 38.0 14-15 26.66348484848485 31.0 11.0 37.0 11.0 38.0 16-17 26.2687012987013 31.0 11.0 37.0 11.0 38.0 18-19 26.4317316017316 31.0 11.0 37.0 11.0 38.0 20-21 26.54926406926407 31.0 11.0 37.0 11.0 38.0 22-23 26.330064935064936 31.0 11.0 37.0 11.0 38.0 24-25 26.423484848484847 31.0 11.0 37.5 11.0 38.0 26-27 26.364025974025974 31.0 11.0 37.5 11.0 38.0 28-29 26.158939393939395 31.0 11.0 38.0 11.0 38.0 30-31 26.131666666666668 31.0 11.0 37.5 11.0 38.0 32-33 26.121060606060606 29.0 11.0 37.0 11.0 38.0 34-35 26.143658008658008 29.0 11.0 37.0 11.0 38.0 36-37 26.026774891774892 27.0 11.0 37.0 11.0 38.0 38-39 25.985887445887446 27.0 11.0 37.0 11.0 38.0 40-41 25.928831168831167 27.0 11.0 37.0 11.0 38.0 42-43 26.145173160173158 29.0 11.0 37.0 11.0 38.0 44-45 25.58742424242424 27.0 11.0 37.0 11.0 38.0 46-47 25.932294372294372 27.0 11.0 37.0 11.0 38.0 48-49 26.07883116883117 27.0 11.0 37.0 11.0 38.0 50-51 26.008398268398267 27.5 11.0 37.0 11.0 38.0 52-53 26.000497835497836 27.0 11.0 37.0 11.0 38.0 54-55 25.87186147186147 27.0 11.0 37.0 11.0 38.0 56-57 25.711601731601732 27.0 11.0 37.0 11.0 38.0 58-59 25.61961038961039 27.0 11.0 37.0 11.0 38.0 60-61 25.74244588744589 27.0 11.0 37.0 11.0 38.0 62-63 25.613896103896103 27.0 11.0 37.0 11.0 38.0 64-65 25.727056277056278 27.0 11.0 37.0 11.0 38.0 66-67 25.805476190476192 27.0 11.0 37.0 11.0 38.0 68-69 25.44365800865801 26.5 11.0 37.0 11.0 38.0 70-71 25.475194805194803 27.0 11.0 37.0 11.0 38.0 72-73 25.48634199134199 27.0 11.0 37.0 11.0 38.0 74-75 25.233852813852813 26.0 11.0 37.0 11.0 38.0 76 24.496017316017316 25.0 11.0 36.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.4299084577337453 1101 2 -1.2135234806561996 1101 3 0.29490249043895744 1101 4 -0.06664186435589414 1101 5 -0.17292825602202555 1101 6 0.4876478412383456 1101 7 -1.1145340216119486 1101 8 -0.4058936937242379 1101 9 0.618005908889284 1101 10-11 -0.45314408757903024 1101 12-13 -1.1690206983062303 1101 14-15 -1.2321397999963004 1101 16-17 0.13482750965208012 1101 18-19 -0.6098681319166133 1101 20-21 -1.2099763245224757 1101 22-23 -0.20010893512456818 1101 24-25 0.27031680585621487 1101 26-27 -0.5545898472258273 1101 28-29 0.6816381208212796 1101 30-31 -0.45481368099768105 1101 32-33 0.24750805862618463 1101 34-35 0.46178726176935925 1101 36-37 -0.791640117932328 1101 38-39 -0.11589370264800891 1101 40-41 -0.40083088617938145 1101 42-43 -0.4700679087834452 1101 44-45 0.1105660399713706 1101 46-47 -1.3069584839700106 1101 48-49 -2.611564648554296 1101 50-51 -2.1780804403245035 1101 52-53 -0.881020977084745 1101 54-55 0.5165551609364165 1101 56-57 -1.4313616131176126 1101 58-59 -0.2982613638045386 1101 60-61 -0.6052179550863279 1101 62-63 0.8788660788168876 1101 64-65 -0.00812465373327953 1101 66-67 -0.3639211013838377 1101 68-69 -1.5599408358930056 1101 70-71 0.17009385942204602 1101 72-73 -0.9878660609373995 1101 74-75 0.7347203548957388 1101 76 -1.6876200944542425 1102 1 2.314313422081355 1102 2 2.3627477057844786 1102 3 2.204917898759451 1102 4 1.4849760092958775 1102 5 1.6471025606189578 1102 6 1.0645353604987449 1102 7 1.395789553118405 1102 8 0.1771571537333898 1102 9 1.6155405776103962 1102 10-11 1.6819868831451608 1102 12-13 -0.6457541343616988 1102 14-15 1.1698632816677978 1102 16-17 1.0161834418554712 1102 18-19 1.6932135321819999 1102 20-21 1.9832440144605776 1102 22-23 1.6846368275872976 1102 24-25 1.4493614899856446 1102 26-27 -1.358133760939232 1102 28-29 -0.29108915190599305 1102 30-31 0.4718427134553238 1102 32-33 -2.193478073885373 1102 34-35 1.8146378010605773 1102 36-37 1.3251549514051142 1102 38-39 1.6032126147633967 1102 40-41 1.4749780506465058 1102 42-43 0.7733835550070012 1102 44-45 -2.6074616950055223 1102 46-47 0.4745515314383084 1102 48-49 0.8577727936645019 1102 50-51 -1.0876335990302017 1102 52-53 1.0434782524992308 1102 54-55 0.5543055461444268 1102 56-57 0.7862038722444851 1102 58-59 1.4943426423587916 1102 60-61 1.2459376689506492 1102 62-63 -1.1815345375159332 1102 64-65 1.1345563940325114 1102 66-67 0.4485596382155457 1102 68-69 -2.8916819761087176 1102 70-71 1.2987533355391498 1102 72-73 2.2640599791242337 1102 74-75 -1.619979182854646 1102 76 -0.9187449018502356 1103 1 -3.629106318696312 1103 2 -2.2282293630091417 1103 3 -1.826487883892593 1103 4 -0.768513522109906 1103 5 -1.7686501811557171 1103 6 -1.8856141908472175 1103 7 -2.3813789413980437 1103 8 -2.2764819290183524 1103 9 -1.3889459627684673 1103 10-11 -2.614374034103097 1103 12-13 -2.0905447624773537 1103 14-15 -0.8393590513332008 1103 16-17 -1.7475254315243909 1103 18-19 -1.7202959394032433 1103 20-21 -1.4702704421695323 1103 22-23 -1.2383442292422124 1103 24-25 -1.5005388091170495 1103 26-27 -1.156862574498554 1103 28-29 -1.666089151905993 1103 30-31 -1.7204553922276276 1103 32-33 -3.3452726900369143 1103 34-35 -0.649843754273423 1103 36-37 -1.5615599040285844 1103 38-39 -1.7223108149474697 1103 40-41 -3.4311784797622664 1103 42-43 -2.3938647002272972 1103 44-45 -3.120048933290665 1103 46-47 -3.3899798743443412 1103 48-49 -0.8653079640623176 1103 50-51 -1.8366365900571218 1103 52-53 -0.4431867524858113 1103 54-55 -1.7722148925395196 1103 56-57 -1.7002118804972888 1103 58-59 -2.1073522728954472 1103 60-61 -2.263907794658518 1103 62-63 -2.769395953268674 1103 64-65 -1.1094615521290052 1103 66-67 -0.44600666288116386 1103 68-69 -2.769299124663057 1103 70-71 -1.4174729854977386 1103 72-73 -1.9778393229694835 1103 74-75 -1.7618037092753873 1103 76 -2.55259335648633 1104 1 0.41676725461360675 1104 2 1.2193123402393233 1104 3 2.4900178228677206 1104 4 0.8682292346943115 1104 5 0.7247650954026668 1104 6 0.5937536757023878 1104 7 0.5172163176010756 1104 8 2.1642555600071063 1104 9 2.5816422725256487 1104 10-11 3.053097432095324 1104 12-13 1.4670715676367827 1104 14-15 2.158226550071543 1104 16-17 1.3512454200998398 1104 18-19 1.6311088015976338 1104 20-21 1.1512177053282713 1104 22-23 1.9879507663679696 1104 24-25 2.0194348519891854 1104 26-27 2.576346380725326 1104 28-29 1.9178660719746041 1104 30-31 1.4484427640498083 1104 32-33 0.8586342458717766 1104 34-35 1.5133476416879468 1104 36-37 1.9124304383770365 1104 38-39 1.9256259717074897 1104 40-41 2.748694215584525 1104 42-43 2.755169540334599 1104 44-45 0.9002539639876481 1104 46-47 2.026284392556448 1104 48-49 2.2775805346207356 1104 50-51 1.9190701702589443 1104 52-53 1.7628053458460577 1104 54-55 1.0491196114113102 1104 56-57 1.8225054153762805 1104 58-59 3.2956328017314185 1104 60-61 2.113506603936738 1104 62-63 2.2864644505943623 1104 64-65 1.2270178157375433 1104 66-67 1.5586026435279692 1104 68-69 -1.4811077287016872 1104 70-71 0.6517763560299166 1104 72-73 1.543341537434376 1104 74-75 1.917081277555166 1104 76 2.895826309887685 1105 1 -0.2883500161753041 1105 2 0.629333662200942 1105 3 -0.8466559511194838 1105 4 0.7125789148648849 1105 5 -0.2703308534246247 1105 6 -0.33183267824217566 1105 7 -0.0015470086249358417 1105 8 1.4798205919900482 1105 9 0.781642272525648 1105 10-11 0.4936091591742162 1105 12-13 1.39201826273273 1105 14-15 0.23669136883486885 1105 16-17 -0.8651724903479199 1105 18-19 1.1784435563950737 1105 20-21 1.6757379611918104 1105 22-23 0.8713196363040048 1105 24-25 0.5469401824795916 1105 26-27 0.8895659969300169 1105 28-29 -0.12680343762027846 1105 30-31 0.6990824228984245 1105 32-33 1.9072483183664453 1105 34-35 1.1449041448862403 1105 36-37 0.4453728690806571 1105 38-39 0.1587816220273197 1105 40-41 -0.646934782283278 1105 42-43 0.37084118212564476 1105 44-45 2.1696569490622757 1105 46-47 0.7060285290170043 1105 48-49 1.3968769098872613 1105 50-51 0.6738676116235531 1105 52-53 0.6981998021360347 1105 54-55 0.1626590570403117 1105 56-57 -0.6579849897409886 1105 58-59 0.39264772710455276 1105 60-61 -0.557815357683733 1105 62-63 -0.39775729780648916 1105 64-65 0.5178493722407431 1105 66-67 0.9380269505642147 1105 68-69 -0.0924083683605339 1105 70-71 0.1928211321493194 1105 72-73 0.3601858871145467 1105 74-75 -0.12566925549387165 1105 76 -0.32528243211658037 1106 1 0.8711347613422618 1106 2 -0.606966103607018 1106 3 -0.7864685974895096 1106 4 -0.7253601952053721 1106 5 0.7790836664817 1106 6 1.2098535044276133 1106 7 -0.308572769749059 1106 8 0.4727948308659258 1106 9 -0.10688231763828426 1106 10-11 0.7715951076519652 1106 12-13 0.12293161167886879 1106 14-15 0.027323687336039626 1106 16-17 0.5118766899799496 1106 18-19 0.022471659439567304 1106 20-21 0.9146138394119525 1106 22-23 -0.14601057446882848 1106 24-25 0.5218816344702226 1106 26-27 -0.35563306630183433 1106 28-29 -0.545187512561732 1106 30-31 -0.3071236660945509 1106 32-33 -0.20375870739467672 1106 34-35 -0.6410443328655155 1106 36-37 -0.30251940258210297 1106 38-39 0.9396949709734557 1106 40-41 0.7140722434778439 1106 42-43 0.8257592149125301 1106 44-45 -0.13608075585575463 1106 46-47 1.0809699810076339 1106 48-49 -0.8076898348434192 1106 50-51 0.9326498130287 1106 52-53 0.5504246264920063 1106 54-55 1.0918159657054147 1106 56-57 0.8514997877765786 1106 58-59 -0.18112276469872768 1106 60-61 0.2444094666722414 1106 62-63 0.32402256167828725 1106 64-65 0.10871588277938571 1106 66-67 -0.03351871688309416 1106 68-69 0.5448281655973091 1106 70-71 0.2215096567394852 1106 72-73 -0.7248258224873254 1106 74-75 -1.1323437285617892 1106 76 -0.4983503478074489 1107 1 -1.9345197884320342 1107 2 -1.9671466690619965 1107 3 -1.3801963237902939 1107 4 -1.0969448946589253 1107 5 -0.6245751598428448 1107 6 -0.8438409598156724 1107 7 -1.0655221639044399 1107 8 -1.9508212299561194 1107 9 -1.6357490318221757 1107 10-11 -1.1682956027305487 1107 12-13 -0.8566359815736853 1107 14-15 -2.4891885483493965 1107 16-17 -1.2274913309276307 1107 18-19 -0.742674456027288 1107 20-21 -1.6389618317688495 1107 22-23 -2.2406886452694934 1107 24-25 -1.3822523641042572 1107 26-27 -1.6296886614550772 1107 28-29 -2.102683354804544 1107 30-31 -2.5656173700622382 1107 32-33 -1.76790696113666 1107 34-35 -1.1923629358591015 1107 36-37 -1.471086758248532 1107 38-39 -0.9746552309747543 1107 40-41 -0.2725041404613293 1107 42-43 -0.9733617164250816 1107 44-45 -1.2484589929667074 1107 46-47 -0.9246132929291662 1107 48-49 -0.8736199845226764 1107 50-51 -0.8602938790596788 1107 52-53 -0.6794399494167607 1107 54-55 -0.2653947731874311 1107 56-57 0.3284539336544583 1107 58-59 -0.6943087946345763 1107 60-61 -1.6086435150336271 1107 62-63 -1.8236372149907538 1107 64-65 -0.5158980397675386 1107 66-67 -1.4722214966407563 1107 68-69 -0.9042096105965634 1107 70-71 -1.651381766401407 1107 72-73 -1.310000448289177 1107 74-75 -1.5731847834441837 1107 76 -2.306855931081383 1108 1 -1.7871427727145388 1108 2 -1.3261995369942277 1108 3 -2.0042012227492627 1108 4 -0.3880247068654974 1108 5 0.18661079647477052 1108 6 -0.5243880102341265 1108 7 -0.31965566053640515 1108 8 1.1518527851490035 1108 9 -0.5451182908546315 1108 10-11 -1.1166524102422848 1108 12-13 -0.7677402885751157 1108 14-15 -0.3677351905212696 1108 16-17 -1.837003476263412 1108 18-19 -1.3207516146310851 1108 20-21 -0.08180731043796641 1108 22-23 -0.7944751323076638 1108 24-25 0.013338572821641748 1108 26-27 -1.500172433653482 1108 28-29 -1.8192581659904974 1108 30-31 -1.4309980599989593 1108 32-33 0.28169499844693036 1108 34-35 -1.0977517907274397 1108 36-37 -0.9879269297120992 1108 38-39 -1.465061436322781 1108 40-41 -0.6657476595468594 1108 42-43 -1.0974686770292834 1108 44-45 0.21684004765382525 1108 46-47 0.07242691935905299 1108 48-49 -1.3191190659678682 1108 50-51 -0.20842615095190453 1108 52-53 -2.0686010026929402 1108 54-55 -2.9847252487946996 1108 56-57 -0.9617073237450136 1108 58-59 -2.001718470078547 1108 60-61 -1.6703908104000291 1108 62-63 -0.7021838571626269 1108 64-65 -1.6535791991878277 1108 66-67 -1.7125766711661683 1108 68-69 -2.514742372384678 1108 70-71 -0.29661548756899236 1108 72-73 -0.11526682918323772 1108 74-75 -1.0358302212886414 1108 76 -1.1460067781930405 1109 1 0.4885407401272168 1109 2 -0.21352348065619964 1109 3 0.041159174930935905 1109 4 0.08442765436068456 1109 5 0.7607615835501669 1109 6 2.055562279741018 1109 7 0.16273870566077875 1109 8 1.8411651298051765 1109 9 -0.09482831570964478 1109 10-11 1.245920083543961 1109 12-13 0.40945523752264634 1109 14-15 0.2782880074903282 1109 16-17 0.4510039802403156 1109 18-19 2.922958908726514E-4 1109 20-21 0.24296485194811268 1109 22-23 0.04842047664013904 1109 24-25 1.3965200144123635 1109 26-27 0.5563727196190946 1109 28-29 -0.23226562249422855 1109 30-31 -0.25722009810997903 1109 32-33 0.5076684864336727 1109 34-35 0.19574448102069653 1109 36-37 1.5046165665596511 1109 38-39 0.49092447917017523 1109 40-41 1.4291156378847916 1109 42-43 -0.27621764140376825 1109 44-45 0.10053930200345107 1109 46-47 -1.6767445802266963 1109 48-49 -0.5711903170034951 1109 50-51 0.14130458641346522 1109 52-53 -0.06818675248581485 1109 54-55 0.3380792251075384 1109 56-57 0.9762587077380012 1109 58-59 -0.25441109642486026 1109 60-61 0.6625627935767717 1109 62-63 -0.622337129739261 1109 64-65 0.1699502125768788 1109 66-67 0.6495815724129557 1109 68-69 1.0615832282781206 1109 70-71 0.2075270145022614 1109 72-73 -1.345486381793016 1109 74-75 0.054372761312848183 1109 76 -1.5820051211922106 1110 1 0.3911142695389813 1110 2 0.13022651934380036 1110 3 -0.7511202368337706 1110 4 -0.46152822799225746 1110 5 -0.31184871056748165 1110 6 -1.3313862496707465 1110 7 0.6158637056607787 1110 8 1.6316063062757635 1110 9 -1.3089827274743513 1110 10-11 0.35253773060278704 1110 12-13 -1.1461513801244116 1110 14-15 -0.41196934545084574 1110 16-17 -0.18548499034791988 1110 18-19 0.9152739135379306 1110 20-21 0.0697111754775257 1110 22-23 0.5686410648754325 1110 24-25 -0.2840866032346945 1110 26-27 -1.602175074498554 1110 28-29 -0.03327665190599305 1110 30-31 -0.34131936281586306 1110 32-33 -0.38203739591926933 1110 34-35 -0.3414797836851875 1110 36-37 -0.16266284520505536 1110 38-39 -1.1809505208298248 1110 40-41 -0.9904615679975635 1110 42-43 -1.1057213178743552 1110 44-45 -0.4100305509377229 1110 46-47 -0.9203107566972832 1110 48-49 -0.1732123758270241 1110 50-51 0.34351046876640723 1110 52-53 -0.9165323407211083 1110 54-55 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-0.2787426759794691 2116 62-63 -0.23134125770623726 2116 64-65 0.46045588853147024 2116 66-67 2.0785031410404073 2116 68-69 1.0797720827672848 2116 70-71 -1.7150736046927868 2116 72-73 0.2697096966383583 2116 74-75 1.2353583134284314 2116 76 1.8418854876829158 2117 1 -0.08194607528860587 2117 2 0.28647651934380036 2117 3 -0.6875426506268738 2117 4 -1.0509678831646703 2117 5 1.224250427363554 2117 6 -1.0032612496707465 2117 7 -0.6734681908909437 2117 8 -2.1852040385518237 2117 9 -0.10801289988814489 2117 10-11 -0.4384105452592806 2117 12-13 -0.019804397365792425 2117 14-15 -1.4017322764853297 2117 16-17 -0.3910345593134359 2117 18-19 -0.29889634508275975 2117 20-21 -2.3082521865914423 2117 22-23 -0.11562617650387708 2117 24-25 -0.8818775515105557 2117 26-27 -1.4025522296709667 2117 28-29 -1.515227082940477 2117 30-31 -0.20904565591931146 2117 32-33 -0.9811753269537533 2117 34-35 -1.2647017664438067 2117 36-37 -1.4740852589981586 2117 38-39 -0.38407552082982477 2117 40-41 -2.017131826618254 2117 42-43 -1.999848473046768 2117 44-45 -1.60965339576531 2117 46-47 -0.38313403255935086 2117 48-49 -1.9560787551373657 2117 50-51 0.06549322738709762 2117 52-53 -0.28776078899696955 2117 54-55 -1.586109415866094 2117 56-57 -1.3946844971301502 2117 58-59 -2.1418350315161376 2117 60-61 -1.007569052265012 2117 62-63 -0.18445680519565144 2117 64-65 0.4671104559845851 2117 66-67 -1.8496577784998252 2117 68-69 -0.7505364471782698 2117 70-71 -0.393385764402403 2117 72-73 -0.8366121424421031 2117 74-75 -1.6091667924396873 2117 76 0.3515648585238118 2118 1 -2.3795588073840968 2118 2 -2.7827542498869704 2118 3 -2.586216390679926 2118 4 -1.830278227992256 2118 5 -0.658242941336713 2118 6 -0.2647997112092071 2118 7 -2.4949536020315293 2118 8 -3.513586001416545 2118 9 0.5354884263718027 2118 10-11 -0.9888084232433663 2118 12-13 -0.2739158032013371 2118 14-15 -2.000671268527771 2118 16-17 -2.6267109518863805 2118 18-19 -1.8644135864620708 2118 20-21 -2.2176686322147816 2118 22-23 -1.2924166274322602 2118 24-25 -2.215697180157772 2118 26-27 -0.4587856514216284 2118 28-29 -0.9526276134444558 2118 30-31 -1.4135549397389404 2118 32-33 0.40834721946534813 2118 34-35 -0.1605903606082677 2118 36-37 -0.5112205375127488 2118 38-39 -0.46484475159905614 2118 40-41 -1.9441875295360234 2118 42-43 -1.209928048643583 2118 44-45 0.2504261798315106 2118 46-47 -1.6181472951588205 2118 48-49 -1.0157604527501007 2118 50-51 -0.6151433773874366 2118 52-53 -2.0292727253364937 2118 54-55 -0.8477805033992496 2118 56-57 -0.5530399347959296 2118 58-59 -2.1227368882800626 2118 60-61 -2.5506725005408732 2118 62-63 -2.6505045505537446 2118 64-65 -1.1920407376493678 2118 66-67 -2.454830192292931 2118 68-69 -1.6165841925363615 2118 70-71 -1.380255790927606 2118 72-73 -2.1969569700283103 2118 74-75 -1.2932516730762948 2118 76 -1.0120956189297665 2119 1 -1.4196168625364898 2119 2 -1.0814480089580876 2119 3 -1.1455777840035815 2119 4 -0.4680140770488599 2119 5 -0.7142661633976708 2119 6 -1.3994876647650862 2119 7 -3.7759405396222405 2119 8 -0.3530635050449895 2119 9 -2.229678482191332 2119 10-11 -2.2311886844915527 2119 12-13 0.2944441387435113 2119 14-15 -1.026650949224429 2119 16-17 -0.704795131857356 2119 18-19 -1.3304513223111272 2119 20-21 -1.8646933056545514 2119 22-23 -0.28690138795475306 2119 24-25 -0.15952882021582937 2119 26-27 -0.46110785751742256 2119 28-29 -1.0835419820946726 2119 30-31 -0.8856884665894462 2119 32-33 -0.7806223015796476 2119 34-35 -2.0836672836851875 2119 36-37 -1.5358939772805265 2119 38-39 -0.5869057095090682 2119 40-41 -2.1430264264881274 2119 42-43 -2.6433097612705794 2119 44-45 -0.4331732396169663 2119 46-47 -0.591006511414264 2119 48-49 -0.08034680978928677 2119 50-51 -1.1293668897241567 2119 52-53 -2.0937139444946915 2119 54-55 -2.5469096761133265 2119 56-57 -0.0052895719512271455 2119 58-59 0.10962885918002385 2119 60-61 -1.8902951420503094 2119 62-63 -1.6629864083186199 2119 64-65 -2.4648999539048084 2119 66-67 -1.302000003613685 2119 68-69 -1.200764163508783 2119 70-71 -1.340197735775206 2119 72-73 -0.4912965926698192 2119 74-75 0.16799651270019567 2119 76 -0.10614496580930322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 64.0 13 334.0 14 704.0 15 995.0 16 1247.0 17 1552.0 18 1681.0 19 1594.0 20 1403.0 21 1083.0 22 809.0 23 581.0 24 437.0 25 337.0 26 329.0 27 279.0 28 293.0 29 296.0 30 345.0 31 423.0 32 500.0 33 698.0 34 945.0 35 1331.0 36 2173.0 37 2664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.991375931007447 36.86136155755912 8.789581427762533 32.35768108367089 2 16.943722943722943 27.27705627705628 31.025974025974023 24.753246753246753 3 17.307359307359306 29.064935064935067 26.805194805194805 26.82251082251082 4 16.74025974025974 24.805194805194805 28.844155844155843 29.61038961038961 5 10.593073593073592 36.01731601731602 31.80952380952381 21.58008658008658 6 16.437229437229437 35.46320346320346 15.354978354978355 32.74458874458874 7 14.4978354978355 41.76190476190476 16.26839826839827 27.47186147186147 8 12.926406926406928 42.497835497835496 15.987012987012985 28.588744588744593 9 14.3982683982684 27.757575757575758 16.597402597402596 41.24675324675325 10-11 14.867965367965368 31.922077922077925 24.515151515151516 28.694805194805195 12-13 14.259740259740258 27.935064935064936 24.298701298701296 33.506493506493506 14-15 14.893939393939393 30.056277056277057 20.7012987012987 34.34848484848485 16-17 17.77705627705628 30.80519480519481 21.794372294372295 29.623376623376625 18-19 16.060606060606062 29.274891774891778 21.560606060606062 33.103896103896105 20-21 15.658008658008656 29.937229437229433 21.426406926406926 32.97835497835498 22-23 18.735930735930737 29.614718614718615 20.564935064935064 31.084415584415588 24-25 16.753246753246753 32.18614718614718 20.227272727272727 30.833333333333336 26-27 15.738095238095237 32.43722943722943 22.38961038961039 29.435064935064936 28-29 17.4978354978355 30.303030303030305 24.21861471861472 27.98051948051948 30-31 16.805194805194805 30.27922077922078 24.841991341991342 28.073593073593074 32-33 15.448051948051948 32.984848484848484 23.20995670995671 28.357142857142858 34-35 15.997835497835498 30.967532467532465 23.625541125541126 29.409090909090907 36-37 15.857142857142856 29.40692640692641 25.017316017316016 29.718614718614717 38-39 16.597402597402596 26.92857142857143 26.800865800865804 29.673160173160174 40-41 14.889932682525595 28.199744583216084 25.738652351782505 31.171670382475813 42-43 12.816017316017318 27.23809523809524 27.45887445887446 32.48701298701299 44-45 13.515151515151516 27.227272727272727 28.46969696969697 30.787878787878785 46-47 14.015151515151514 29.608225108225106 26.33982683982684 30.03679653679654 48-49 13.803030303030303 30.571428571428573 24.29004329004329 31.335497835497833 50-51 13.68644342951146 29.968181129461673 23.64336890408883 32.70200653693803 52-53 12.976190476190478 29.484848484848484 24.26839826839827 33.270562770562776 54-55 12.716450216450218 29.837662337662334 23.783549783549784 33.66233766233766 56-57 14.63852813852814 29.764069264069263 22.980519480519483 32.616883116883116 58-59 13.971861471861471 29.313852813852815 23.11038961038961 33.603896103896105 60-61 13.261904761904761 28.39177489177489 23.584415584415584 34.76190476190476 62-63 13.632034632034632 29.504329004329005 22.6991341991342 34.16450216450217 64-65 14.543290043290044 30.662337662337663 20.515151515151516 34.27922077922078 66-67 13.38961038961039 32.616883116883116 20.116883116883116 33.87662337662338 68-69 14.4004329004329 32.209956709956714 19.68181818181818 33.70779220779221 70-71 13.7987012987013 33.19264069264069 19.660173160173162 33.34848484848485 72-73 14.277056277056277 32.5 18.857142857142858 34.365800865800864 74-75 14.653679653679655 32.84632034632035 18.794372294372295 33.705627705627705 76 13.99134199134199 32.56277056277057 18.98701298701299 34.45887445887446 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 2.0 11 4.0 12 5.0 13 2.5 14 1.0 15 3.5 16 4.0 17 3.0 18 10.0 19 11.5 20 15.0 21 24.0 22 26.5 23 34.5 24 47.5 25 55.0 26 59.0 27 79.0 28 104.0 29 113.0 30 150.5 31 210.5 32 260.0 33 287.0 34 363.5 35 496.5 36 609.5 37 666.0 38 772.5 39 956.5 40 1086.5 41 1244.0 42 1349.0 43 1386.0 44 1497.5 45 1598.0 46 1624.0 47 1561.5 48 1507.0 49 1489.5 50 1464.0 51 1364.5 52 1169.5 53 1025.5 54 977.0 55 871.0 56 694.5 57 558.5 58 493.0 59 454.0 60 362.0 61 265.5 62 222.0 63 189.0 64 139.5 65 102.5 66 68.5 67 55.0 68 51.5 69 35.5 70 25.5 71 28.0 72 27.0 73 19.0 74 12.5 75 13.0 76 9.5 77 8.5 78 8.0 79 5.0 80 3.0 81 2.5 82 4.0 83 4.0 84 3.0 85 3.0 86 2.5 87 1.0 88 1.5 89 3.0 90 3.5 91 2.5 92 2.0 93 3.0 94 2.0 95 0.0 96 0.0 97 0.0 98 2.0 99 5.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6103896103896104 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0021645021645021645 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0021645021645021645 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 23100.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.32034632034632 #Duplication Level Percentage of deduplicated Percentage of total 1 92.17757960548636 82.33333333333334 2 5.195560509862841 9.281385281385282 3 1.7690108079290456 4.740259740259741 4 0.5040469151359472 1.800865800865801 5 0.20355740803567102 0.9090909090909091 6 0.09693209906460525 0.5194805194805194 7 0.014539814859690787 0.09090909090909091 8 0.009693209906460524 0.06926406926406926 9 0.014539814859690787 0.11688311688311688 >10 0.014539814859690787 0.13852813852813853 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012987012987012988 0.0 2 0.0 0.0 0.0 0.012987012987012988 0.0 3 0.0 0.0 0.0 0.012987012987012988 0.0 4 0.0 0.0 0.0 0.012987012987012988 0.0 5 0.0 0.0 0.0 0.012987012987012988 0.0 6 0.0 0.0 0.0 0.012987012987012988 0.0 7 0.0 0.0 0.0 0.012987012987012988 0.0 8 0.0 0.0 0.0 0.012987012987012988 0.0 9 0.0 0.0 0.0 0.017316017316017316 0.0 10 0.0 0.0 0.0 0.017316017316017316 0.0 11 0.0 0.0 0.0 0.021645021645021644 0.0 12 0.0 0.0 0.0 0.021645021645021644 0.0 13 0.0 0.0 0.0 0.021645021645021644 0.0 14 0.0 0.0 0.0 0.025974025974025976 0.0 15 0.0 0.0 0.0 0.025974025974025976 0.0 16 0.0 0.0 0.0 0.030303030303030304 0.0 17 0.0 0.0 0.0 0.030303030303030304 0.0 18 0.0 0.0 0.0 0.03463203463203463 0.0 19 0.0 0.0 0.0 0.04329004329004329 0.0 20 0.0 0.0 0.0 0.04329004329004329 0.0 21 0.0 0.0 0.0 0.06060606060606061 0.0 22 0.0 0.0 0.0 0.06060606060606061 0.0 23 0.0 0.0 0.0 0.06493506493506493 0.0 24 0.0 0.0 0.0 0.06493506493506493 0.0 25 0.0 0.0 0.0 0.06493506493506493 0.0 26 0.0 0.0 0.0 0.06493506493506493 0.0 27 0.0 0.0 0.0 0.08225108225108226 0.0 28 0.0 0.0 0.0 0.09956709956709957 0.0 29 0.0 0.0 0.0 0.12121212121212122 0.0 30 0.0 0.0 0.0 0.12121212121212122 0.0 31 0.0 0.0 0.0 0.13852813852813853 0.0 32 0.0 0.0 0.0 0.1471861471861472 0.0 33 0.0 0.0 0.0 0.20346320346320346 0.0 34 0.0 0.0 0.0 0.2857142857142857 0.0 35 0.0 0.0 0.0 0.31601731601731603 0.0 36 0.0 0.0 0.0 0.41125541125541126 0.0 37 0.0 0.0 0.0 0.6277056277056277 0.0 38 0.0 0.0 0.0 0.7965367965367965 0.0 39 0.0 0.0 0.0 1.0303030303030303 0.0 40 0.0 0.0 0.0 1.2294372294372293 0.0 41 0.0 0.0 0.0 1.4675324675324675 0.0 42 0.0 0.0 0.0 1.7792207792207793 0.0 43 0.0 0.0 0.0 2.116883116883117 0.0 44 0.0 0.0 0.0 2.3593073593073592 0.0 45 0.0 0.0 0.0 2.675324675324675 0.0 46 0.0 0.0 0.0 2.9523809523809526 0.0 47 0.0 0.0 0.0 3.220779220779221 0.0 48 0.0 0.0 0.0 3.4761904761904763 0.0 49 0.0 0.0 0.0 3.70995670995671 0.0 50 0.0 0.0 0.0 3.9740259740259742 0.0 51 0.0 0.0 0.0 4.212121212121212 0.0 52 0.0 0.0 0.0 4.523809523809524 0.0 53 0.0 0.0 0.0 4.83982683982684 0.0 54 0.0 0.0 0.0 5.077922077922078 0.0 55 0.0 0.0 0.0 5.3982683982683985 0.0 56 0.0 0.0 0.0 5.636363636363637 0.0 57 0.0 0.0 0.0 5.857142857142857 0.0 58 0.0 0.0 0.0 6.064935064935065 0.0 59 0.0 0.0 0.0 6.329004329004329 0.0 60 0.0 0.0 0.0 6.554112554112554 0.0 61 0.0 0.0 0.0 6.770562770562771 0.0 62 0.0 0.0 0.0 7.086580086580087 0.0 63 0.0 0.0 0.0 7.259740259740259 0.0 64 0.0 0.0 0.0 7.5021645021645025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCTGT 15 0.0021989502 69.98701 35 CCCGCCA 20 0.0068715815 52.49026 53 TCGCCGT 20 0.0068715815 52.49026 53 CGAATTG 20 0.0068715815 52.49026 25 GACGAAT 20 0.0068715815 52.49026 23 GAGAGGA 20 0.0068715815 52.49026 23 ACGAATT 20 0.0068715815 52.49026 24 TTTACAC 30 5.7652E-4 46.658005 2 CTTTACA 35 0.0011545226 40.518795 1 CGACGAA 45 0.0042263092 31.105337 22 TGTAGAT 50 0.007073734 27.994806 38 >>END_MODULE