FastQCFastQC Report
Wed 25 May 2016
SRR1780370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23100
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT281112.168831168831169No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT251510.887445887445887RNA PCR Primer, Index 27 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC9734.212121212121212No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC4231.831168831168831No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG2921.2640692640692641No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2481.0735930735930737No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1400.6060606060606061No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1400.6060606060606061No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1320.5714285714285714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1260.5454545454545455No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG910.393939393939394No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT870.37662337662337664No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT840.36363636363636365No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT820.354978354978355No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC780.33766233766233766No Hit
CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC760.329004329004329No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT650.2813852813852814No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT580.2510822510822511No Hit
CTTATCACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC530.22943722943722947No Hit
TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC520.22510822510822512No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG440.19047619047619047No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGT430.18614718614718614No Hit
CTTATACACATCTCCGAGCCCACGCGACTTGACCCTATCTCGTATGCCGT430.18614718614718614No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG380.1645021645021645No Hit
ATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGTCTT310.1341991341991342TruSeq Adapter, Index 4 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT300.12987012987012986No Hit
ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT280.12121212121212122RNA PCR Primer, Index 27 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGTCTT260.11255411255411256No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGT260.11255411255411256No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCG250.10822510822510822No Hit
TCTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT240.1038961038961039No Hit
ATACACATCTCCGAGCCCACGAGAATTGACCCTATCTCGTATGCCGTCTT240.1038961038961039No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAATT150.002197332470.023
AGCTCGT207.765569E-570.034
TAGCTCG207.765569E-570.033
AGAATTG150.002197332470.024
TCTCGTC150.002197332470.035
GCTCGTA207.765569E-570.035
CTAGCTC207.765569E-570.032
CGAGAAT150.002197332470.022
TTTACAC1350.070.02
CCTAGCT207.765569E-570.031
CTTTACA1350.067.407411
CTATACA700.060.01
AATACAC252.3421593E-455.9999964
TTACACA1700.055.588233
ACAAAGA200.006866541752.570
GCCTTGA200.006866541752.526
CTTTATA407.1673094E-752.52
GTCTGCC200.006866541752.539
ATTACAC200.006866541752.54
TCTGCCG200.006866541752.540
Produced by FastQC (version 0.11.5)