FastQCFastQC Report
Wed 25 May 2016
SRR1780369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234339
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA850.036.233711
TAGATCC250.0023445135.2135138
ACTACCG250.00236425335.1533935
GTAGAAC401.809079E-533.01266535
CGTGTAT401.809079E-533.01266535
AATTAGT300.005701028329.36970519
TTTACAC1500.029.3007492
AGGGACA752.1736923E-929.3007492
CTTTACA1600.027.4987981
AGTAACC407.034429E-427.48705332
TATACAC9350.026.3236683
TTATACA9600.025.6381532
CTTATAC10050.024.7352391
GGTGGTC1700.024.5714144
ACCACGT450.001391734724.45382536
TTGGGCC450.00140566424.4120774
TTACACA2000.023.0743393
GGGCATA500.002558994322.02256813
ACGTTCT500.002568548822.00844439
AGCTCCA500.002568548822.00844436
Produced by FastQC (version 0.11.5)