FastQCFastQC Report
Wed 25 May 2016
SRR1780369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234339
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT3513414.992809562215422No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT2737311.680940859182638Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC96664.12479356829209No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC50362.149023423331157No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG43741.8665266985008897No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT26701.139375008001229No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC20370.8692535173402636No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC14680.6264428882943086No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG13250.565420181873269No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG10050.42886587379821545No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9110.3887530458011684No Hit
CTTAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC7750.33071746486927056No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC6580.2807897959793291No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5530.2359829136422021No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5470.23342252036579486No Hit
CTTATCACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC4750.2026978010489078No Hit
TCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT3180.13570084364958457No Hit
ACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTTCT2990.12759293160762827Illumina PCR Primer Index 10 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT2830.12076521620387559No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC2570.10967017867277748No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2510.10710978539637021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCCT254.433358E-543.99765437
ATCGTAT207.845701E-443.9976536
ACTCGCA207.845701E-443.9976521
CACCGTG207.845701E-443.997655
CCGTCTT32250.042.98362744
TTTACAC10100.040.7391932
CTTTACA10200.040.564171
CTTATAA1650.040.0149421
ATAACAC1600.039.8813824
GCCGTCT35100.039.43081343
CTATACA6300.038.0777131
GGTATGA357.2574367E-637.7203241
TTACACA11100.037.0689963
CCTGTTG301.2967121E-436.67253541
CTGTTGG301.2967121E-436.67253542
CGTGGAA250.002349550835.19812418
TATATCG250.002349550835.19812433
TTACTCG250.002349550835.19812419
CCGTATT250.002349550835.19812414
ATATCGT250.002349550835.19812434
Produced by FastQC (version 0.11.5)