FastQCFastQC Report
Wed 25 May 2016
SRR1780368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11249
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAC150.002168215669.9955326
ATCATCA200.006776248652.49665559
TCCAACA200.006776248652.49665512
TTTACAC200.006776248652.4966552
CGACGAA305.634027E-446.6636922
TTATACA953.6379788E-1240.5237272
GCCGACG501.6055904E-434.9977720
GCTGCCG601.1132448E-534.99776517
TATACAC1153.092282E-1133.4761243
CCGACGA751.3257104E-632.66458521
CTGCCGA651.926083E-532.30563418
CTTATAC1106.8394E-1031.9588261
ACATCTG1107.185008E-1031.8161538
TGCCGAC604.6815866E-429.16480419
CGCTGCC853.521196E-628.8216916
GACGCTG853.521196E-628.8216914
CATCTGA1102.6373527E-828.6345399
TGCACGA500.00691652227.99821528
ACGCTGC807.9463E-526.24832715
TGACGCT1259.124415E-825.19839313
Produced by FastQC (version 0.11.5)