##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780368_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11249 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.03529202595786 12.0 12.0 23.0 12.0 32.0 2 18.47675348919904 12.0 11.0 23.0 11.0 32.0 3 19.156102764690196 21.0 11.0 23.0 11.0 32.0 4 18.734376389012358 11.0 11.0 23.0 11.0 33.0 5 19.498088718997245 21.0 11.0 27.0 11.0 33.0 6 21.572673126500135 21.0 11.0 31.0 11.0 36.0 7 21.997955373811006 22.0 11.0 32.0 11.0 36.0 8 22.266601475686727 23.0 11.0 34.0 11.0 36.0 9 22.22348653213619 21.0 12.0 33.0 12.0 36.0 10-11 20.894746199662194 21.0 11.0 31.0 11.0 35.5 12-13 20.73597653124722 21.0 11.0 29.5 11.0 34.0 14-15 20.55240465819184 21.0 11.0 29.5 11.0 34.0 16-17 20.02844697306427 16.5 11.0 27.5 11.0 34.0 18-19 20.33531869499511 21.0 11.0 28.0 11.0 34.0 20-21 20.323539870210688 21.0 11.0 29.0 11.0 34.0 22-23 19.939194595075115 16.0 11.0 29.0 11.0 34.0 24-25 20.062850031113875 16.0 11.0 29.0 11.0 34.0 26-27 19.567739354609298 21.0 11.0 27.0 10.5 34.0 28-29 19.046537469997332 11.0 11.0 26.0 11.0 34.0 30-31 19.0392034847542 11.0 11.0 26.0 11.0 34.0 32-33 19.484265268023826 16.0 11.0 26.5 11.0 34.0 34-35 19.523379855987198 16.0 11.0 26.5 11.0 34.0 36-37 19.263267846030757 11.0 11.0 26.0 11.0 34.0 38-39 18.607831807271758 11.0 11.0 26.0 11.0 34.0 40-41 18.97857587341097 11.0 11.0 26.0 11.0 34.0 42-43 19.095075117788248 11.0 11.0 26.0 11.0 34.0 44-45 19.120766290336917 11.0 11.0 26.0 11.0 34.0 46-47 19.0680949417726 11.0 11.0 26.0 11.0 34.0 48-49 19.28518090496933 16.0 11.0 26.0 11.0 34.0 50-51 19.23317628233621 11.0 11.0 26.0 11.0 34.0 52-53 19.373188727886923 15.0 11.0 26.0 11.0 34.0 54-55 19.355142679349274 15.5 11.0 26.0 11.0 34.0 56-57 18.813316739265712 11.0 11.0 26.0 11.0 33.5 58-59 18.970397368654993 16.0 11.0 26.0 11.0 33.5 60-61 19.091119210596496 15.0 11.0 26.0 11.0 33.0 62-63 18.879633745221795 15.0 11.0 26.0 11.0 33.0 64-65 19.103253622544226 15.0 11.0 26.0 11.0 33.0 66-67 19.31389456840608 19.0 11.0 26.0 11.0 33.0 68-69 18.926082318428307 15.0 11.0 26.0 11.0 33.0 70-71 19.11156547248644 19.0 11.0 26.0 11.0 33.0 72-73 19.074628855898304 19.0 11.0 25.5 11.0 33.0 74-75 18.906169437283314 15.0 11.0 25.5 11.0 33.0 76 18.282958485198684 11.0 11.0 25.0 11.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.25728248128906017 1101 2 -0.9199388576245866 1101 3 0.393702891782965 1101 4 -0.42661257992465806 1101 5 -2.0953567855300896 1101 6 -1.802990198832724 1101 7 -5.173054167419938 1101 8 -2.831129637407063 1101 9 -1.782983551653892 1101 10-11 0.8970240825009554 1101 12-13 0.8137388175642748 1101 14-15 0.825963620627391 1101 16-17 2.0040429257963197 1101 18-19 0.3333335368098638 1101 20-21 0.373416482673786 1101 22-23 0.3365210142101347 1101 24-25 -0.783373349610855 1101 26-27 0.3942234721843185 1101 28-29 -1.2107117994024605 1101 30-31 -0.33542524770597737 1101 32-33 -0.10483410233290869 1101 34-35 1.6230822253990986 1101 36-37 -0.3423997470430642 1101 38-39 1.1026887326683692 1101 40-41 -1.2815782524959545 1101 42-43 0.9635760567396652 1101 44-45 0.5393273919220718 1101 46-47 0.21601210072860155 1101 48-49 -0.004449716159577122 1101 50-51 -1.8053177592032803 1101 52-53 0.23874615161745893 1101 54-55 -0.8717749939388568 1101 56-57 -0.8508438172815822 1101 58-59 -0.7775852496267355 1101 60-61 0.1318058314152566 1101 62-63 0.2566810980475793 1101 64-65 0.524738924658557 1101 66-67 0.6994070251978997 1101 68-69 -0.5789133225792824 1101 70-71 -0.5591973570845603 1101 72-73 -0.7001650097955832 1101 74-75 -0.13546493829424477 1101 76 -3.077566876826623 1102 1 -1.2771763804933052 1102 2 1.6675942988740893 1102 3 0.3074959952312426 1102 4 2.274978932011681 1102 5 1.614192286087949 1102 6 2.19170476137948 1102 7 0.20758243735459914 1102 8 0.6410189037069962 1102 9 -0.9540710848103906 1102 10-11 -0.7402438220083276 1102 12-13 0.6220942552300599 1102 14-15 -0.9605085279137242 1102 16-17 -1.631898718766017 1102 18-19 -1.5844383464792635 1102 20-21 -1.3997930663978302 1102 22-23 -1.7370864128455672 1102 24-25 -0.5187844901944096 1102 26-27 -0.2138667134920702 1102 28-29 -1.5489080858746114 1102 30-31 -0.36924487635318926 1102 32-33 -1.4072213702374192 1102 34-35 -1.927315652584987 1102 36-37 -0.8404103571226429 1102 38-39 -0.8621653787374655 1102 40-41 -2.9314456265012616 1102 42-43 -2.188280707186067 1102 44-45 -0.9082853401734177 1102 46-47 -1.2873035491387697 1102 48-49 -0.5740783633744293 1102 50-51 -3.8358217379831228 1102 52-53 -0.603428914695538 1102 54-55 -1.829997805609942 1102 56-57 -1.3667589366449775 1102 58-59 0.4472158113812199 1102 60-61 -0.3370270598314242 1102 62-63 -0.7671915809975154 1102 64-65 1.005508155427787 1102 66-67 -1.5432985450938759 1102 68-69 -2.102786001624377 1102 70-71 1.8957098047191536 1102 72-73 -1.3991040018380225 1102 74-75 -1.8642447791430463 1102 76 0.17442251309380197 1103 1 0.015131311814389647 1103 2 1.2547737860535761 1103 3 0.7741626618979076 1103 4 0.6262609832937294 1103 5 -0.8781154062197452 1103 6 -1.757013187338469 1103 7 -0.17190474213258256 1103 8 -1.4230836603955659 1103 9 1.5997750690357613 1103 10-11 0.836679254914749 1103 12-13 1.1746583577941578 1103 14-15 1.5920555746503808 1103 16-17 -0.43446282133011493 1103 18-19 1.1040231919822752 1103 20-21 2.59123257462781 1103 22-23 0.5577853820262249 1103 24-25 1.2235232021132845 1103 26-27 -2.185661585286944 1103 28-29 -0.2796773166438413 1103 30-31 2.724344867236553 1103 32-33 -0.3008111138271623 1103 34-35 1.026530501261167 1103 36-37 1.9736922069799263 1103 38-39 0.7147576981856112 1103 40-41 -1.7493943444499784 1103 42-43 3.063001344095987 1103 44-45 0.6853044034163247 1103 46-47 0.4447477329125107 1103 48-49 -0.2010014402975031 1103 50-51 -0.7576166097779939 1103 52-53 -0.029069940336565736 1103 54-55 0.6328227072105719 1103 56-57 0.18708721720117438 1103 58-59 -1.1091944450290363 1103 60-61 0.9116908888865218 1103 62-63 -2.2030890168949497 1103 64-65 0.5580722579918884 1103 66-67 0.2028553010599694 1103 68-69 -0.9886834375218108 1103 70-71 0.2316072406165901 1103 72-73 2.057306254572236 1103 74-75 1.3639603490620793 1103 76 -1.061474922803633 1104 1 1.4739548412261527 1104 2 0.733205158602594 1104 3 -1.0748569459452284 1104 4 -1.2815821539611711 1104 5 1.8042375349567266 1104 6 1.4586730871713343 1104 7 -2.9915125852698345 1104 8 -0.5897503270622337 1104 9 -0.6825778721407083 1104 10-11 1.057267490208865 1104 12-13 0.9060309068137684 1104 14-15 2.1175457707288103 1104 16-17 1.9959293355326295 1104 18-19 0.26284672139404464 1104 20-21 -0.2391595822349366 1104 22-23 2.7097461663399507 1104 24-25 -0.06177091553377423 1104 26-27 -1.4140929578359653 1104 28-29 1.7938520951208652 1104 30-31 -0.46879238766540965 1104 32-33 0.28056143519244614 1104 34-35 2.4765305012611663 1104 36-37 2.3021235795289456 1104 38-39 1.2294635805385532 1104 40-41 0.5957036947657102 1104 42-43 0.28456997154696495 1104 44-45 0.315696560279072 1104 46-47 0.8133751838929015 1104 48-49 1.1872338538201426 1104 50-51 1.25806966473181 1104 52-53 0.41014574593794606 1104 54-55 2.1926266287791982 1104 56-57 1.8017930995541178 1104 58-59 1.1417859471278256 1104 60-61 1.896004614376718 1104 62-63 1.6253423556540696 1104 64-65 1.5835624540703215 1104 66-67 0.7293258892952679 1104 68-69 -0.28378147673749865 1104 70-71 0.5031758680675686 1104 72-73 0.9504435094741943 1104 74-75 -0.1968239646634089 1104 76 -1.065396491431084 1105 1 0.2294170261001014 1105 2 0.09286902414881482 1105 3 -1.6210754333401844 1105 4 -0.32611996908721963 1105 5 0.05045602235168545 1105 6 0.06203443170914724 1105 7 0.37571430548646845 1105 8 -1.3326074699193775 1105 9 1.2426322118929072 1105 10-11 -1.4276064593709634 1105 12-13 1.7698964530322527 1105 14-15 -0.37699204439723744 1105 16-17 0.32029908343178803 1105 18-19 1.3825946205537072 1105 20-21 -0.13495790156266807 1105 22-23 -1.0541193798785358 1105 24-25 0.8663803449704268 1105 26-27 -0.08804253766789572 1105 28-29 1.1488941119275893 1105 30-31 0.3148210577127415 1105 32-33 -0.012715875731924342 1105 34-35 0.19795907268973778 1105 36-37 -1.9167839834962663 1105 38-39 -1.2066708732429596 1105 40-41 -0.8446324396880769 1105 42-43 -0.41080817971353767 1105 44-45 0.004352022463944394 1105 46-47 -1.2219189337541572 1105 48-49 -0.20100144029750666 1105 50-51 -0.3742832764446611 1105 52-53 -0.3100223212889439 1105 54-55 -0.8624153880275252 1105 56-57 -0.1414842113702548 1105 58-59 -2.0830039688385575 1105 60-61 -1.0002138730182395 1105 62-63 2.063577649771716 1105 64-65 -0.5324039324843 1105 66-67 -1.1828589846543167 1105 68-69 -0.6720167708551408 1105 70-71 0.29351200252135357 1105 72-73 -0.4045985073325298 1105 74-75 -0.20984917474744336 1105 76 -1.0519511132798236 1106 1 0.3344861505240644 1106 2 0.5472469043331465 1106 3 0.7429798661989828 1106 4 0.06282012307867646 1106 5 0.6154329808770314 1106 6 1.769868533091639 1106 7 -0.9450230217024718 1106 8 0.24895935035712213 1106 9 -0.9131281567706893 1106 10-11 -1.316546551536863 1106 12-13 -2.0038362658617537 1106 14-15 -1.288051952231342 1106 16-17 -1.5059681976741999 1106 18-19 -0.15888003382417537 1106 20-21 -0.9953265651571357 1106 22-23 -1.2513544029200112 1106 24-25 -0.8184122817576842 1106 26-27 -0.35716696163103023 1106 28-29 -0.5861289295470655 1106 30-31 1.3125169102473038 1106 32-33 -0.5002734794185599 1106 34-35 -1.321856595513026 1106 36-37 -0.1660927392566336 1106 38-39 -0.08362939858858098 1106 40-41 -0.7031577853101929 1106 42-43 -0.5214072580545519 1106 44-45 -0.5329751664761488 1106 46-47 0.7877584856006834 1106 48-49 -0.9655175693297622 1106 50-51 -0.019444566767241156 1106 52-53 0.9461988768677365 1106 54-55 -0.28223105623028744 1106 56-57 -0.9064611698956 1106 58-59 -0.08069982137312337 1106 60-61 -0.6651908315435868 1106 62-63 -1.5202933179702214 1106 64-65 -1.201067526954347 1106 66-67 0.36737143009222706 1106 68-69 0.12798322914485638 1106 70-71 1.003650251369276 1106 72-73 -0.36312385295465077 1106 74-75 0.5085840049760577 1106 76 -1.336743740007936 1107 1 1.2484646451477204 1107 2 -1.1634080321282418 1107 3 0.7771929649282114 1107 4 2.008079165111912 1107 5 0.36127853317419323 1107 6 1.1520777217524376 1107 7 -0.5931168633447008 1107 8 0.16782543051352405 1107 9 1.6997750690357627 1107 10-11 0.7988004670359601 1107 12-13 -0.31322043008462686 1107 14-15 1.763267695862499 1107 16-17 -0.8632507001179945 1107 18-19 2.120689858648941 1107 20-21 0.8018386352338709 1107 22-23 1.535058109298955 1107 24-25 0.9689777475678305 1107 26-27 1.0128232631979053 1107 28-29 2.4021408651743386 1107 30-31 0.5940418369335205 1107 32-33 -1.105356568372617 1107 34-35 0.7416820164126818 1107 36-37 0.33732857061628607 1107 38-39 -0.3685756351477245 1107 40-41 0.562726867671234 1107 42-43 0.2948195259141677 1107 44-45 1.2671225852345103 1107 46-47 2.532626520791297 1107 48-49 0.2808167415206775 1107 50-51 2.8832924811310967 1107 52-53 0.022445211178588664 1107 54-55 1.3419136163014826 1107 56-57 0.8931478232617813 1107 58-59 0.2286843428497498 1107 60-61 1.6647211919168257 1107 62-63 1.2060018921959568 1107 64-65 2.1914055913252213 1107 66-67 0.37861287681754874 1107 68-69 -0.008380407218780306 1107 70-71 1.316455725465076 1107 72-73 -0.0820876848217047 1107 74-75 1.4836573187590467 1107 76 3.1021614408327274 1108 1 1.854525251208326 1108 2 0.018410149689938038 1108 3 1.0499202376554848 1108 4 0.43232158935433773 1108 5 -1.7296305577348967 1108 6 -0.12064955097483221 1108 7 0.679610409382569 1108 8 0.6223708850589773 1108 9 0.8209871902478838 1108 10-11 1.495770164005659 1108 12-13 0.5655674487032485 1108 14-15 0.8693283019231082 1108 16-17 1.8185674817001853 1108 18-19 -0.9096131716540867 1108 20-21 -0.016343182947945678 1108 22-23 2.3229368971777404 1108 24-25 0.9841292627193461 1108 26-27 1.452217202591843 1108 28-29 0.1900196530531275 1108 30-31 -0.1635339206422337 1108 32-33 1.5007040376879885 1108 34-35 0.3174395921702562 1108 36-37 0.9282376615253796 1108 38-39 1.646575880003791 1108 40-41 0.0930298979742652 1108 42-43 1.022092253186898 1108 44-45 1.236819554931479 1108 46-47 1.2144447026094802 1108 48-49 1.280816741520674 1108 50-51 -0.6924650946264812 1108 52-53 -0.41694872821535256 1108 54-55 -1.1429348685470053 1108 56-57 1.6355720656860235 1108 58-59 -0.6804065662411567 1108 60-61 -0.19891517171953765 1108 62-63 -0.4606647744707111 1108 64-65 -0.46010956018992744 1108 66-67 1.5755825737872442 1108 68-69 -1.0841379829763547 1108 70-71 2.149789058798408 1108 72-73 1.4179123151782953 1108 74-75 1.3472936823954136 1108 76 -1.4129900743187847 1109 1 -0.27405787737480125 1109 2 -0.7055865743067855 1109 3 0.1318203195555654 1109 4 0.7271618841946328 1109 5 -0.1727100008143374 1109 6 0.8736174432921615 1109 7 1.4289961587683209 1109 8 -0.42758816490007234 1109 9 -1.5164411471804549 1109 10-11 0.3704630386985315 1109 12-13 0.5467304298662299 1109 14-15 1.4303438629386669 1109 16-17 0.6191407822734867 1109 18-19 2.264834002793087 1109 20-21 -0.15966832627308847 1109 22-23 -1.4309533567125108 1109 24-25 -1.3872876086975268 1109 26-27 1.2138879642626073 1109 28-29 0.5041064671399411 1109 30-31 0.24146198435366983 1109 32-33 1.4077474447313953 1109 34-35 2.456260230990896 1109 36-37 -0.0353168020290866 1109 38-39 -0.23794500451709055 1109 40-41 2.0893443942887586 1109 42-43 1.3922806233752638 1109 44-45 -0.04082172270980067 1109 46-47 0.48348647165124703 1109 48-49 -1.6307311700272358 1109 50-51 -0.2256346277960155 1109 52-53 -1.4173582286248525 1109 54-55 0.9958857702736346 1109 56-57 -0.8885884584745014 1109 58-59 0.8687334828988931 1109 60-61 0.8391683663639995 1109 62-63 0.28790197409603735 1109 64-65 0.15987405979369385 1109 66-67 1.167720165924834 1109 68-69 0.8441994453610739 1109 70-71 0.5703459793553272 1109 72-73 0.4023512996172798 1109 74-75 0.6040504391521715 1109 76 0.9457322844035723 1110 1 0.2588813118143882 1110 2 -0.3973095472797574 1110 3 -0.3175040047687574 1110 4 0.5970943166270644 1110 5 2.3593845937802556 1110 6 1.5763201459948633 1110 7 0.8926785912007524 1110 8 1.8789996729377663 1110 9 4.137275069035763 1110 10-11 0.008554254914749038 1110 12-13 0.4069500244608264 1110 14-15 1.9649722413170458 1110 16-17 -1.1042544879967835 1110 18-19 0.9383981919822766 1110 20-21 1.2985242412944764 1110 22-23 2.8182020486928927 1110 24-25 2.9860232021132838 1110 26-27 -0.4398282519536103 1110 28-29 0.8609476833561587 1110 30-31 1.5670532005698874 1110 32-33 -2.796644447160496 1110 34-35 -0.7422194987388337 1110 36-37 1.7497338736465906 1110 38-39 -0.4258673018143888 1110 40-41 0.9495639888833551 1110 42-43 2.57133467742932 1110 44-45 1.090512736749659 1110 46-47 -0.12504393375415646 1110 48-49 -0.869751440297506 1110 50-51 0.15696672355533892 1110 52-53 -0.8113616070032315 1110 54-55 -0.09321895945609526 1110 56-57 0.9277122172011758 1110 58-59 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0.056170242364309786 2116 64-65 -2.8641499642303323 2116 66-67 0.043596041800711305 2116 68-69 -1.3905352893736627 2116 70-71 1.350125759135107 2116 72-73 -1.1612122639462825 2116 74-75 1.088034423136154 2116 76 0.8496361883074748 2117 1 1.8766697733528481 2117 2 0.9368250681048593 2117 3 1.1536498413850893 2117 4 0.5442097012424476 2117 5 1.4603461322417957 2117 6 5.19170476137948 2117 7 3.0152747450469057 2117 8 2.987172749860843 2117 9 0.1997750690357627 2117 10-11 0.9136023318378257 2117 12-13 -0.12790574476994365 2117 14-15 -1.0951239125291075 2117 16-17 -1.8242064110737068 2117 18-19 -0.4305921926331102 2117 20-21 3.254053087448323 2117 22-23 -2.352471028230184 2117 24-25 1.1542924328825173 2117 26-27 -2.1754051750305337 2117 28-29 -1.5681388551053814 2117 30-31 0.9769089698006574 2117 32-33 0.20816324514719753 2117 34-35 1.5342228089534728 2117 36-37 -0.01348728019956269 2117 38-39 1.0032192366471513 2117 40-41 -0.8737533188089515 2117 42-43 1.5809500620447068 2117 44-45 -0.004439186327264366 2117 46-47 -1.729611241446463 2117 48-49 1.9643831750871072 2117 50-51 -0.585821737983121 2117 52-53 -0.0649673762340015 2117 54-55 0.20846373285159814 2117 56-57 1.3832410633550225 2117 58-59 0.7549081190735301 2117 60-61 1.4322037093993423 2117 62-63 2.867423803617868 2117 64-65 -1.186799536879903 2117 66-67 1.918239916444584 2117 68-69 1.5702909214525498 2117 70-71 -1.0273671183577697 2117 72-73 -2.4183347710687926 2117 74-75 1.0972936823954136 2117 76 2.943653282324572 2118 1 -1.7771763804933052 2118 2 -1.9477903165105275 2118 3 0.42288061061585935 2118 4 0.6595943166270644 2118 5 0.8064999783956388 2118 6 -1.80829523862052 2118 7 -1.3693406395684775 2118 8 -2.0897503270622337 2118 9 0.6997750690357627 2118 10-11 1.2020638702993622 2118 12-13 -1.4932903601545604 2118 14-15 -0.38358545099064756 2118 16-17 0.32963974277245 2118 18-19 3.0501770381361233 2118 20-21 2.8309761643713998 2118 22-23 0.6859905102313526 2118 24-25 0.7504462790363604 2118 26-27 -0.2138667134920702 2118 28-29 -0.9527542397207647 2118 30-31 -0.6192448763531893 2118 32-33 0.477394014377964 2118 34-35 -1.196546421815757 2118 36-37 -0.2442565109687962 2118 38-39 0.1570653904933046 2118 40-41 -0.046830241885878365 2118 42-43 -0.28443455333990997 2118 44-45 0.14940696751888893 2118 46-47 -1.3065343183695433 2118 48-49 -0.6125399018359658 2118 50-51 -0.5473601995215809 2118 52-53 -0.27650583777246496 2118 54-55 0.2469252713131347 2118 56-57 1.2293949095088692 2118 58-59 0.21644658061198996 2118 60-61 1.816819094014729 2118 62-63 0.23280841900248106 2118 64-65 1.2170466169662504 2118 66-67 -1.0432985450938759 2118 68-69 -1.6412475400859137 2118 70-71 -0.7773671183577697 2118 72-73 1.1585883058542876 2118 74-75 1.6165244516261836 2118 76 2.405191743863032 2119 1 -0.9485050518219751 2119 2 -2.2694686381888474 2119 3 -1.4652312774960308 2119 4 -0.8858602288274824 2119 5 -3.396297224401561 2119 6 -3.5600434903687734 2119 7 1.5432467730189359 2119 8 -4.498841236153144 2119 9 -3.118406749146054 2119 10-11 -1.8905934723579776 2119 12-13 -1.7450386119028103 2119 14-15 0.626904059498866 2119 16-17 -0.2874931243604202 2119 18-19 0.13584137380045647 2119 20-21 -0.7511916677964336 2119 22-23 0.0653611396019862 2119 24-25 -2.0310222524321695 2119 26-27 -0.547782797408157 2119 28-29 -1.256950043916568 2119 30-31 -2.0423217994301126 2119 32-33 -0.18868990170594913 2119 34-35 -1.5916513169206539 2119 36-37 -1.4732774899897727 2119 38-39 -1.2170604836325687 2119 40-41 1.7066662616106285 2119 42-43 -0.8491198680252268 2119 44-45 -1.6495440814321611 2119 46-47 -1.399191661026883 2119 48-49 -2.9919105312065994 2119 50-51 -0.28337418553557114 2119 52-53 -0.22755478882141134 2119 54-55 0.9934287678166314 2119 56-57 -2.008367328253371 2119 58-59 -2.248588384422977 2119 60-61 -0.7898242626286311 2119 62-63 0.2817594679535347 2119 64-65 -3.020715620795988 2119 66-67 -0.5835083353036659 2119 68-69 -0.05383495267332705 2119 70-71 0.1422133012226503 2119 72-73 1.092154739420721 2119 74-75 -1.6754335903318598 2119 76 0.6324644711357585 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 2.0 12 45.0 13 236.0 14 553.0 15 850.0 16 1105.0 17 1276.0 18 1432.0 19 1392.0 20 1205.0 21 919.0 22 657.0 23 469.0 24 308.0 25 217.0 26 152.0 27 113.0 28 76.0 29 60.0 30 51.0 31 25.0 32 19.0 33 16.0 34 17.0 35 14.0 36 18.0 37 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.121767916256598 27.851838597119084 27.816050818645433 37.210342667978885 2 14.819095030669393 24.908880789403504 33.82522890923638 26.446795270690725 3 4.9697724039829305 27.933854907539118 40.25604551920342 26.840327169274538 4 15.112454440394702 26.90016890390257 26.25122233087386 31.736154324828874 5 4.284825317806027 47.28420304027024 22.224197706462796 26.20677393546093 6 6.258890469416785 33.970483641536276 12.695590327169274 47.07503556187766 7 5.023115220483641 36.46870554765292 11.175320056899004 47.33285917496444 8 3.8759000800071117 47.81758378522535 12.863365632500667 35.44315050226687 9 7.173971019646191 34.847542003733665 11.103209174148814 46.87527780247133 10-11 6.756156102764691 34.571961952173524 22.90870299582185 35.76317894923993 12-13 6.61836607698462 30.207129522624232 22.80202684683083 40.372477553560316 14-15 10.836518801671259 33.53631433905236 17.188194506178327 38.43897235309805 16-17 13.912966173267547 32.222074054318355 18.23354225007779 35.631417522336314 18-19 10.525380033780781 29.869321717485995 18.26829051471242 41.3370077340208 20-21 9.703084718641657 31.38501200106676 18.07271757489555 40.839185705396034 22-23 15.570272913147834 31.056093875011115 15.050226686816606 38.323406525024446 24-25 11.561027646901948 35.878744777313536 15.245799626633477 37.31442794915104 26-27 10.165348030936084 35.834296381900614 18.886123210952086 35.11423237621122 28-29 12.752244643968352 31.082762912258865 22.984265268023822 33.180727175748956 30-31 11.925504489287936 31.78949239932438 23.833229620410705 32.451773490976976 32-33 9.770191581099702 36.56932035382495 20.513846290616527 33.14664177445882 34-35 9.80976086763268 33.94079473730998 20.45959640856965 35.789847986487686 36-37 10.316472575340029 30.896079651524577 23.41096986398791 35.37647790914748 38-39 10.650308930079566 26.492421211717115 27.02582566564431 35.83144419255901 40-41 8.951906836163214 29.375944528402524 23.682105076006756 37.990043559427505 42-43 5.267134856431683 27.180193795003998 28.433638545648503 39.11903280291582 44-45 6.613921237443328 29.318161614365724 28.460307582896256 35.607609565294695 46-47 7.480664947995377 32.41176993510535 24.2554893768335 35.852075740065786 48-49 7.67623788781225 34.08302960263134 20.17957151746822 38.06116099208818 50-51 7.796595101569098 32.26207938836289 19.455927456994264 40.485398053073745 52-53 6.325006667259311 31.780602720241802 20.344030580496046 41.55036003200284 54-55 7.200640056893946 32.056182771801936 19.010578718108277 41.73259845319584 56-57 10.116454795981864 32.10507600675616 18.277180193795004 39.50128900346698 58-59 8.662992265979199 31.651702373544317 18.539425726731267 41.14587963374522 60-61 6.498355409369721 29.55818294959552 19.53951462352209 44.40394701751267 62-63 8.072187402764813 31.217495666088812 17.922389651953594 42.787927279192786 64-65 8.427415770290693 33.211841052538006 14.979109254155926 43.38163392301538 66-67 6.712006045250478 38.009512379428365 13.78850513401787 41.489976441303284 68-69 9.06747266423682 37.212196639701304 13.570095119566183 40.15023557649569 70-71 8.07627344652858 38.07449551071206 13.87678904791537 39.97244199484399 72-73 8.000711174326607 38.10116454795982 11.996621921948618 41.90150235576496 74-75 8.742999377722464 37.3944350608943 12.618899457729576 41.24366610365366 76 7.209529735976532 38.75900080007112 13.450084451951286 40.581385012001064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.0 13 1.0 14 3.0 15 4.0 16 8.5 17 13.0 18 8.0 19 7.5 20 16.0 21 20.0 22 15.0 23 15.0 24 26.0 25 32.0 26 39.0 27 47.5 28 58.5 29 68.0 30 88.0 31 109.0 32 128.5 33 147.0 34 173.5 35 231.0 36 287.0 37 312.0 38 342.5 39 399.0 40 457.0 41 539.5 42 590.0 43 637.0 44 671.0 45 699.0 46 740.0 47 729.5 48 706.0 49 696.5 50 700.0 51 652.5 52 571.0 53 504.5 54 472.0 55 437.0 56 400.5 57 353.0 58 307.0 59 273.5 60 208.0 61 154.0 62 132.0 63 119.5 64 101.0 65 78.0 66 55.0 67 49.0 68 39.5 69 25.0 70 20.0 71 20.0 72 18.5 73 13.0 74 10.0 75 11.0 76 11.0 77 9.0 78 7.0 79 7.0 80 5.0 81 2.5 82 2.5 83 3.0 84 2.5 85 2.5 86 1.5 87 0.0 88 2.5 89 4.5 90 5.0 91 3.5 92 1.0 93 1.0 94 1.0 95 2.5 96 4.0 97 3.5 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6400568939461285 2 0.0 3 0.008889679082585119 4 0.0 5 0.0 6 0.008889679082585119 7 0.008889679082585119 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.004444839541292559 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004444839541292559 34-35 0.0 36-37 0.0 38-39 0.004444839541292559 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.004444839541292559 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.004444839541292559 64-65 0.0 66-67 0.004444839541292559 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 11249.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.60885412036625 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80365908076752 99.41328118054939 2 0.12494422132976349 0.24891101431238333 3 0.04462293618920125 0.13334518623877678 4 0.00892458723784025 0.035558716330340474 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.00892458723784025 0.08000711174326607 >10 0.00892458723784025 0.08889679082585118 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008889679082585119 0.0 2 0.0 0.0 0.0 0.008889679082585119 0.0 3 0.0 0.0 0.0 0.008889679082585119 0.0 4 0.0 0.0 0.0 0.008889679082585119 0.0 5 0.0 0.0 0.0 0.008889679082585119 0.0 6 0.0 0.0 0.0 0.008889679082585119 0.0 7 0.0 0.0 0.0 0.008889679082585119 0.0 8 0.0 0.0 0.0 0.008889679082585119 0.0 9 0.0 0.0 0.0 0.008889679082585119 0.0 10 0.0 0.0 0.0 0.008889679082585119 0.0 11 0.0 0.0 0.0 0.008889679082585119 0.0 12 0.0 0.0 0.0 0.008889679082585119 0.0 13 0.0 0.0 0.0 0.008889679082585119 0.0 14 0.0 0.0 0.0 0.017779358165170237 0.0 15 0.0 0.0 0.0 0.017779358165170237 0.0 16 0.0 0.0 0.0 0.017779358165170237 0.0 17 0.0 0.0 0.0 0.017779358165170237 0.0 18 0.0 0.0 0.0 0.026669037247755358 0.0 19 0.0 0.0 0.0 0.035558716330340474 0.0 20 0.0 0.0 0.0 0.04444839541292559 0.0 21 0.0 0.0 0.0 0.053338074495510715 0.0 22 0.0 0.0 0.0 0.053338074495510715 0.0 23 0.0 0.0 0.0 0.053338074495510715 0.0 24 0.0 0.0 0.0 0.053338074495510715 0.0 25 0.0 0.0 0.0 0.06222775357809583 0.0 26 0.0 0.0 0.0 0.06222775357809583 0.0 27 0.0 0.0 0.0 0.07111743266068095 0.0 28 0.0 0.0 0.0 0.08889679082585118 0.0 29 0.0 0.0 0.0 0.0977864699084363 0.0 30 0.0 0.0 0.0 0.10667614899102143 0.0 31 0.0 0.0 0.0 0.10667614899102143 0.0 32 0.0 0.0 0.0 0.10667614899102143 0.0 33 0.0 0.0 0.0 0.10667614899102143 0.0 34 0.0 0.0 0.0 0.11556582807360655 0.0 35 0.0 0.0 0.0 0.1422348653213619 0.0 36 0.0 0.0 0.0 0.16001422348653213 0.0 37 0.0 0.0 0.0 0.17779358165170236 0.0 38 0.0 0.0 0.0 0.21335229798204286 0.0 39 0.0 0.0 0.0 0.22224197706462798 0.0 40 0.0 0.0 0.0 0.24891101431238333 0.0 41 0.0 0.0 0.0 0.26669037247755356 0.0 42 0.0 0.0 0.0 0.2844697306427238 0.0 43 0.0 0.0 0.0 0.3289181260556494 0.0 44 0.0 0.0 0.0 0.34669748422081964 0.0 45 0.0 0.0 0.0 0.4178149168815006 0.0 46 0.0 0.0 0.0 0.45337363321184104 0.0 47 0.0 0.0 0.0 0.49782202862476665 0.0 48 0.0 0.0 0.0 0.5156013867899368 0.0 49 0.0 0.0 0.0 0.5689394612854476 0.0 50 0.0 0.0 0.0 0.6222775357809583 0.0 51 0.0 0.0 0.0 0.6400568939461285 0.0 52 0.0 0.0 0.0 0.6578362521112988 0.0 53 0.0 0.0 0.0 0.7111743266068095 0.0 54 0.0 0.0 0.0 0.7200640056893947 0.0 55 0.0 0.0 0.0 0.7556227220197351 0.0 56 0.0 0.0 0.0 0.7734020801849053 0.0 57 0.0 0.0 0.0 0.8000711174326607 0.0 58 0.0 0.0 0.0 0.817850475597831 0.0 59 0.0 0.0 0.0 0.8356298337630012 0.0 60 0.0 0.0 0.0 0.8445195128455862 0.0 61 0.0 0.0 0.0 0.8800782291759267 0.0 62 0.0 0.0 0.0 0.897857587341097 0.0 63 0.0 0.0 0.0 0.9334163036714375 0.0 64 0.0 0.0 0.0 0.977864699084363 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAAAC 15 0.0021682156 69.99553 26 ATCATCA 20 0.0067762486 52.496655 59 TCCAACA 20 0.0067762486 52.496655 12 TTTACAC 20 0.0067762486 52.496655 2 CGACGAA 30 5.634027E-4 46.66369 22 TTATACA 95 3.6379788E-12 40.523727 2 GCCGACG 50 1.6055904E-4 34.99777 20 GCTGCCG 60 1.1132448E-5 34.997765 17 TATACAC 115 3.092282E-11 33.476124 3 CCGACGA 75 1.3257104E-6 32.664585 21 CTGCCGA 65 1.926083E-5 32.305634 18 CTTATAC 110 6.8394E-10 31.958826 1 ACATCTG 110 7.185008E-10 31.816153 8 TGCCGAC 60 4.6815866E-4 29.164804 19 CGCTGCC 85 3.521196E-6 28.82169 16 GACGCTG 85 3.521196E-6 28.82169 14 CATCTGA 110 2.6373527E-8 28.634539 9 TGCACGA 50 0.006916522 27.998215 28 ACGCTGC 80 7.9463E-5 26.248327 15 TGACGCT 125 9.124415E-8 25.198393 13 >>END_MODULE