FastQCFastQC Report
Wed 25 May 2016
SRR1780368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11249
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT286025.424482176193443No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT221719.708418526091208Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC7086.293892790470264No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC3593.1913947906480575No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG3503.1113876789047916No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT2632.3379855987198863Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT1481.3156725042225976No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1040.9245266245888524No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1030.9156369455062673No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC900.8000711174326608No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG790.7022846475242244No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT780.6933949684416393No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT670.595608498533203No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT600.5333807449551071No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC590.524491065872522No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT580.5156013867899368No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG510.4533736332118411No Hit
TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC500.44448395412925595No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT500.44448395412925595No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT430.3822562005511601No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC380.3378078051382345No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG370.3289181260556494No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG370.3289181260556494No Hit
CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT340.30224908880789403Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT320.2844697306427238No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG310.27558005156013865No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT280.24891101431238333No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT250.22224197706462798No Hit
TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT250.22224197706462798No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTC240.21335229798204286No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC240.21335229798204286No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGT230.20446261889945772No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCG220.1955729398168726No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT210.18668326073428748Illumina PCR Primer Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT210.18668326073428748Illumina PCR Primer Index 1 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCG200.17779358165170236No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTAGTATGCCGT200.17779358165170236No Hit
ATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTGCTT180.16001422348653213Illumina PCR Primer Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT180.16001422348653213TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGT180.16001422348653213No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTC170.15112454440394701No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGTCTT170.15112454440394701No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT170.15112454440394701No Hit
CTTATACACATCTCCGAGCCCAAGAGACTGGTCACAATCTCGTATGCCGT160.1422348653213619No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT150.13334518623877678No Hit
CTTATACACATCTCCGAGCACACGAGACTGGTCACAATCTCGTATGCCGT140.12445550715619166No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATTTCGTATGCCGT140.12445550715619166No Hit
ATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGTCTT140.12445550715619166Illumina PCR Primer Index 1 (95% over 22bp)
ATACACATCTCCGCGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT140.12445550715619166Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGT140.12445550715619166No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCACGTATGCCGT140.12445550715619166No Hit
ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT130.11556582807360655TruSeq Adapter, Index 1 (95% over 22bp)
CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC130.11556582807360655No Hit
CTTATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGT120.10667614899102143No Hit
CTTATACACATATCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT120.10667614899102143No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGTCTT120.10667614899102143TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGTGACTGGTCACAATCTCGTATGCCGT120.10667614899102143No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGC120.10667614899102143No Hit
CTTCTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT120.10667614899102143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT252.661529E-670.000011
GTCACAC150.002167666870.028
AAAACTC150.002167666870.070
CTTTACA900.066.1111151
TTTACAC900.066.1111152
CTATACA501.0913936E-1163.0000041
CTTTATA307.853239E-658.3333322
TTTATAC307.853239E-658.3333323
CTTATAC5100.056.9607851
TTACACA1050.056.6666643
AAATATA252.2899342E-456.00000468
TTATACA5450.053.3027532
ACAAAAT200.006774539652.50000467
GAGCACA200.006774539652.50000416
CGAGCAC200.006774539652.50000415
CCGAGCA200.006774539652.50000414
AGCACAC200.006774539652.50000417
GCACACG200.006774539652.50000418
GAAAAAC305.632255E-446.66666460
TATACAC6350.046.2992133
Produced by FastQC (version 0.11.5)