##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780368_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11249 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76344563961241 34.0 34.0 34.0 31.0 34.0 2 33.372477553560316 34.0 34.0 34.0 33.0 34.0 3 33.50057782914037 34.0 34.0 34.0 34.0 34.0 4 33.488843452751354 34.0 34.0 34.0 34.0 34.0 5 33.562361098764335 34.0 34.0 34.0 34.0 34.0 6 37.364121255222685 38.0 37.0 38.0 37.0 38.0 7 36.91599253266957 38.0 37.0 38.0 35.0 38.0 8 36.93777224642191 38.0 37.0 38.0 35.0 38.0 9 36.627344652858035 38.0 37.0 38.0 34.0 38.0 10-11 36.29287047737577 38.0 37.0 38.0 33.0 38.0 12-13 35.99057694017246 38.0 36.5 38.0 33.0 38.0 14-15 35.906925060005335 38.0 36.0 38.0 31.0 38.0 16-17 35.803893679438175 38.0 36.0 38.0 31.0 38.0 18-19 35.97848697662015 38.0 36.5 38.0 32.0 38.0 20-21 35.89225708951906 38.0 36.0 38.0 31.0 38.0 22-23 35.82780691617033 38.0 36.0 38.0 32.5 38.0 24-25 34.43759445284025 37.0 34.0 38.0 28.0 38.0 26-27 34.66810383145169 37.0 34.0 38.0 26.0 38.0 28-29 34.83923015379145 37.0 34.5 38.0 27.0 38.0 30-31 34.6152102409103 37.0 34.5 38.0 26.5 38.0 32-33 35.29735976531247 37.0 36.0 38.0 30.5 38.0 34-35 34.09587518890568 37.0 35.0 38.0 24.0 38.0 36-37 33.536847719797315 37.0 34.0 38.0 22.5 38.0 38-39 31.192906036092097 36.0 27.0 37.0 11.0 38.0 40-41 33.04142590452484 36.5 32.5 37.5 23.0 38.0 42-43 34.70406258334074 37.0 34.5 38.0 28.5 38.0 44-45 35.0890745844075 37.0 35.0 38.0 29.0 38.0 46-47 35.05822739799093 37.0 35.0 38.0 27.0 38.0 48-49 34.527424659969775 37.0 34.5 38.0 26.0 38.0 50-51 34.46101875722286 37.0 34.0 38.0 26.0 38.0 52-53 34.0732509556405 37.0 34.0 38.0 24.0 38.0 54-55 34.50537825584496 37.0 34.5 38.0 25.5 38.0 56-57 34.397101964619075 37.0 34.0 38.0 26.0 38.0 58-59 34.1144990665837 37.0 34.0 38.0 24.0 38.0 60-61 34.14414614632412 37.0 34.0 38.0 25.0 38.0 62-63 34.91065872522002 37.0 35.0 38.0 27.0 38.0 64-65 34.80038225620055 37.0 35.0 38.0 27.0 38.0 66-67 32.54129255933861 36.5 31.0 38.0 21.0 38.0 68-69 28.095919637301094 32.5 21.5 37.0 11.0 38.0 70-71 23.75553382522891 25.0 11.0 33.5 10.5 37.0 72-73 19.892790470264025 21.0 11.0 25.0 10.5 34.0 74-75 19.12423326517913 19.0 11.0 25.0 11.0 34.0 76 19.493732776246777 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5311345797434797 1101 2 0.6455605938903304 1101 3 -0.020049201342459355 1101 4 0.10793503618043587 1101 5 0.1596293069654422 1101 6 -1.822532327952473 1101 7 -0.402548465529037 1101 8 0.3631176313986799 1101 9 -0.12590001827733488 1101 10-11 0.16711017952654572 1101 12-13 0.7967110059176719 1101 14-15 -0.39778328339147606 1101 16-17 -0.03134818826919883 1101 18-19 -1.301652121363034 1101 20-21 -0.28039334373994507 1101 22-23 0.18747835509540778 1101 24-25 -1.1211819542976897 1101 26-27 -2.2705365557450534 1101 28-29 0.892430444334515 1101 30-31 -0.14643759746226692 1101 32-33 -2.754226035677796 1101 34-35 -0.8911382226628177 1101 36-37 -0.10851400949400158 1101 38-39 -1.8819172254161067 1101 40-41 1.3727674434369206 1101 42-43 -0.32415247534776626 1101 44-45 -0.4226333975177141 1101 46-47 0.9568523984761867 1101 48-49 -4.396867457772643 1101 50-51 -3.082338600138467 1101 52-53 -6.237078619662256 1101 54-55 -3.8954821994938165 1101 56-57 -2.0716414723315353 1101 58-59 -3.3300367750394386 1101 60-61 -2.19233883946697 1101 62-63 -3.039488788157815 1101 64-65 -3.195499665951722 1101 66-67 -5.0460422234179525 1101 68-69 -3.020159696184077 1101 70-71 -1.1094604763434361 1101 72-73 -1.6636251454702347 1101 74-75 -0.8852543688326726 1101 76 0.24467044257876935 1102 1 -2.3229117680723945 1102 2 -1.508285559955823 1102 3 -0.037290580652801 1102 4 0.10262999639263626 1102 5 -0.40270491054118907 1102 6 -0.22438909187820144 1102 7 -0.6465802957677624 1102 8 0.4029054298071699 1102 9 0.9258241196536972 1102 10-11 0.5092852458395356 1102 12-13 0.30533169557284623 1102 14-15 0.1672034540090621 1102 16-17 -0.726971530444267 1102 18-19 -1.7300340842277535 1102 20-21 0.784593393660586 1102 22-23 -0.24156673774278659 1102 24-25 -7.814152776578858 1102 26-27 -3.957539208264947 1102 28-29 -1.1645987333843166 1102 30-31 1.7700080258799105 1102 32-33 -0.5314143645902618 1102 34-35 1.1055461274698075 1102 36-37 -4.134376078459518 1102 38-39 0.1465973634433091 1102 40-41 1.0836427750019055 1102 42-43 1.7023727235912247 1102 44-45 1.7451384857714132 1102 46-47 2.074226403781225 1102 48-49 -6.063976211088288 1102 50-51 -2.5803492102180456 1102 52-53 -0.5746117761609284 1102 54-55 1.7331650153602993 1102 56-57 1.6200030900026832 1102 58-59 0.10166083769265555 1102 60-61 -7.673108070236204 1102 62-63 -3.2463853398819538 1102 64-65 0.2580812359050455 1102 66-67 3.4599259463433185 1102 68-69 -0.6567644707198852 1102 70-71 -3.4841289113566987 1102 72-73 -1.4567285937460959 1102 74-75 -1.51058593381941 1102 76 2.190293784753834 1103 1 1.2309343857737574 1103 2 0.3122272605569947 1103 3 0.3627094193471976 1103 4 0.48724538100802306 1103 5 0.03319252535624173 1103 6 0.06022629273718394 1103 7 -0.0876059367934019 1103 8 0.4849567118584517 1103 9 0.025824119653698574 1103 10-11 0.2849262714805576 1103 12-13 -0.6113349710938252 1103 14-15 0.5056649924705923 1103 16-17 0.18072077724804103 1103 18-19 0.29432489013122165 1103 20-21 -0.8936117345445496 1103 22-23 0.27253582635977125 1103 24-25 -3.448768161194245 1103 26-27 -1.0498469005726463 1103 28-29 -0.6133166821022655 1103 30-31 0.9777003335722156 1103 32-33 1.498072814896922 1103 34-35 -0.09573592381224216 1103 36-37 -1.8843760784595176 1103 38-39 -1.06237699553105 1103 40-41 -0.988152096792966 1103 42-43 -1.0040375328190265 1103 44-45 1.5733436139765402 1103 46-47 1.7896110191658323 1103 48-49 -1.8819249290370053 1103 50-51 0.9670866872178507 1103 52-53 1.1882087366595826 1103 54-55 0.5729086051038905 1103 56-57 1.4174389874385724 1103 58-59 0.66191724794907 1103 60-61 -7.142338839466969 1103 62-63 -1.796385339881951 1103 64-65 0.4401325179563216 1103 66-67 -1.047766361348991 1103 68-69 -0.38625165020706476 1103 70-71 0.7209992937715093 1103 72-73 -0.9233952604127644 1103 74-75 -0.3990474722809516 1103 76 -1.0507318562718062 1104 1 -0.34553620246153827 1104 2 0.49850177036091736 1104 3 0.462709419347199 1104 4 -1.33628403075668 1104 5 0.0724082116307514 1104 6 0.18963805744306939 1104 7 0.839845043598757 1104 8 0.5477018098976671 1104 9 0.9258241196536972 1104 10-11 -1.504289414793945 1104 12-13 0.30533169557284623 1104 14-15 1.0880179336470661 1104 16-17 0.6689560713656846 1104 18-19 0.9011876352292632 1104 20-21 -0.08870977376022893 1104 22-23 0.38626131655585283 1104 24-25 0.15417301527634208 1104 26-27 0.7325060406038304 1104 28-29 2.234722533584012 1104 30-31 1.121817980631043 1104 32-33 0.9912100697988748 1104 34-35 1.2356366252073627 1104 36-37 1.8215062744816564 1104 38-39 3.1013484946650287 1104 40-41 2.5598871188933074 1104 42-43 1.367531094631957 1104 44-45 0.17726518260398905 1104 46-47 1.719022783871722 1104 48-49 0.3455260513551508 1104 50-51 1.5474788440805938 1104 52-53 0.9921303052870343 1104 54-55 -0.6084639439157229 1104 56-57 -0.3551100321692644 1104 58-59 -2.2920043206783802 1104 60-61 0.7782493958271459 1104 62-63 0.8124381895298143 1104 64-65 1.4911129101131877 1104 66-67 0.1375277562980699 1104 68-69 4.350022859596855 1104 70-71 2.6866855682813124 1104 72-73 0.8962125827244911 1104 74-75 0.4068348806602273 1104 76 -0.38210440529141465 1105 1 0.9595058143451851 1105 2 -0.06872512039538492 1105 3 -0.19443343779565936 1105 4 0.20153109529373836 1105 5 -0.033474141310421146 1105 6 0.3173691498800437 1105 7 0.8171559679685032 1105 8 -1.0674242405225058 1105 9 -0.031318737489158366 1105 10-11 -0.026978490424198753 1105 12-13 -0.23038259014144558 1105 14-15 -0.38004929324369385 1105 16-17 -1.8264220798948188 1105 18-19 -0.393770347964022 1105 20-21 -0.3912307821635892 1105 22-23 -0.34651179268784915 1105 24-25 0.03694612452003554 1105 26-27 -0.8212754720012114 1105 28-29 0.8200166512310645 1105 30-31 1.163414619286506 1105 32-33 0.9575966244207237 1105 34-35 0.2995021714258499 1105 36-37 1.2156239215404838 1105 38-39 -1.5099960431500996 1105 40-41 0.6166098079689348 1105 42-43 -0.03498991377141181 1105 44-45 0.07096266159558695 1105 46-47 0.6253253048801213 1105 48-49 -0.3914487385608183 1105 50-51 0.3575628776940434 1105 52-53 -0.34988650143564826 1105 54-55 -0.20090091870563498 1105 56-57 -0.9420848220852349 1105 58-59 -0.1285589425271212 1105 60-61 0.5362325891044577 1105 62-63 -3.7178139113105253 1105 64-65 -0.1265341487103413 1105 66-67 -0.5120520756346991 1105 68-69 -1.1886326025880152 1105 70-71 1.347189769961986 1105 72-73 -0.2281571651746681 1105 74-75 -0.6990474722809488 1105 76 -0.017398522938471217 1106 1 -0.15293658196817717 1106 2 0.4520122067935546 1106 3 0.36593522579881466 1106 4 0.48724538100802306 1106 5 0.011687149012161058 1106 6 0.43441984112428145 1106 7 -0.8574984099116847 1106 8 0.7924835935788792 1106 9 0.5548563777182167 1106 10-11 0.701592938147229 1106 12-13 1.748880082669615 1106 14-15 -0.16530274946489243 1106 16-17 1.0726562611190076 1106 18-19 1.6744324170129374 1106 20-21 1.5327323514769589 1106 22-23 1.1101702349619273 1106 24-25 1.051231838805755 1106 26-27 1.8501530994273594 1106 28-29 1.8167908447794545 1106 30-31 1.0293132367980249 1106 32-33 1.3953846428539052 1106 34-35 1.5193178396286129 1106 36-37 2.470462631217906 1106 38-39 1.0429993485549751 1106 40-41 1.8516328494435896 1106 42-43 1.8438119295465611 1106 44-45 2.3276446892453606 1106 46-47 1.6492884385206779 1106 48-49 2.0514084042963248 1106 50-51 -0.809257398803652 1106 52-53 1.5215420699929183 1106 54-55 0.4626935513404433 1106 56-57 1.213675546578358 1106 58-59 1.7834226242931592 1106 60-61 1.7592740637588378 1106 62-63 2.0439372407632064 1106 64-65 -0.8684696325813093 1106 66-67 2.660298154780044 1106 68-69 3.6583720057069122 1106 70-71 0.8849777883951617 1106 72-73 -2.343825367939644 1106 74-75 -0.734531343248694 1106 76 -3.4561082003578285 1107 1 1.149116203955579 1107 2 -0.839287890958154 1107 3 -0.2948663382285588 1107 4 0.09330598706863213 1107 5 -0.14862565646193815 1107 6 0.054165686676576286 1107 7 0.7123940632065953 1107 8 -0.2847402578385214 1107 9 -1.210539516709936 1107 10-11 -0.32871009215580216 1107 12-13 0.418968059209206 1107 14-15 0.281422568228173 1107 16-17 1.3458722923995552 1107 18-19 0.5473551931615219 1107 20-21 0.4139640230312125 1107 22-23 0.0876873415112911 1107 24-25 -0.009374221800307225 1107 26-27 -1.0740893248150627 1107 28-29 0.9624408936553124 1107 30-31 0.19891245478434172 1107 32-33 1.8298909967151005 1107 34-35 1.3951731670968428 1107 36-37 1.9186542245707798 1107 38-39 2.8558048226507715 1107 40-41 1.3482115395706629 1107 42-43 0.9308109520294536 1107 44-45 0.8809193715522952 1107 46-47 0.40930798886280684 1107 48-49 1.3089841618720826 1107 50-51 0.9610260811572502 1107 52-53 1.5366935851444339 1107 54-55 0.47442375661903213 1107 56-57 2.2522874722870654 1107 58-59 1.0043414903733137 1107 60-61 1.6864490393209053 1107 62-63 1.5414934479968352 1107 64-65 1.7825567603805652 1107 66-67 -1.5523118158944413 1107 68-69 0.9819301679747525 1107 70-71 -2.680515857743643 1107 72-73 1.5735744365569353 1107 74-75 -0.22328989652337228 1107 76 -1.314368219908168 1108 1 -0.48724743240806134 1108 2 -0.23322728489754496 1108 3 0.18998214661992563 1108 4 0.33573022949287434 1108 5 -0.11832262615890698 1108 6 -0.6428040102931192 1108 7 -0.16639381558128008 1108 8 -2.012012985111248 1108 9 -0.48326678943720935 1108 10-11 -0.32871009215580216 1108 12-13 0.9644226046637527 1108 14-15 0.675361962167564 1108 16-17 -0.12382467729741364 1108 18-19 0.4109915567978817 1108 20-21 1.3382064472736346 1108 22-23 -0.5335247797008265 1108 24-25 1.4603227478966616 1108 26-27 0.7289409782152347 1108 28-29 -2.0678621366477117 1108 30-31 -0.24048148460960306 1108 32-33 1.3753455421696472 1108 34-35 0.7588095307332097 1108 36-37 1.0701693760859357 1108 38-39 2.0073199741659202 1108 40-41 0.4997266910858187 1108 42-43 -0.7055526843341795 1108 44-45 0.09304058367351331 1108 46-47 0.04567162522644708 1108 48-49 0.2786811315690514 1108 50-51 0.2792078993390632 1108 52-53 1.2033602518110982 1108 54-55 -0.874061091865812 1108 56-57 -0.005288285288699512 1108 58-59 1.9134323994642202 1108 60-61 2.1409944938663656 1108 62-63 2.117251023754406 1108 64-65 1.4643749421987522 1108 66-67 0.1598093962267697 1108 68-69 0.4819301679747525 1108 70-71 1.1376659604381771 1108 72-73 1.6947865577690564 1108 74-75 0.6100434368099599 1108 76 -3.2840651896051405 1109 1 -2.425822370982999 1109 2 -0.03011508178534683 1109 3 0.40865536529314284 1109 4 0.027785921548563408 1109 5 0.15030964247335987 1109 6 -0.4749088423979515 1109 7 -0.03535368454114973 1109 8 0.45072247762421824 1109 9 1.1014997953293744 1109 10-11 0.8367280732823659 1109 12-13 1.122899263140411 1109 14-15 1.4083676951732969 1109 16-17 1.114504561031822 1109 18-19 1.2938744396807706 1109 20-21 1.44467655374374 1109 22-23 0.8743376281615696 1109 24-25 -1.0163357287618098 1109 26-27 0.5258287751030366 1109 28-29 -0.9826860514716387 1109 30-31 1.4966192524911364 1109 32-33 1.3958205626446656 1109 34-35 -0.40744763552395824 1109 36-37 1.8318401377566929 1109 38-39 0.39347886032480517 1109 40-41 -0.5358998445407153 1109 42-43 1.1441606653791752 1109 44-45 1.0647850554179854 1109 46-47 1.2369083164631363 1109 48-49 2.024381377269293 1109 50-51 0.6842038043349774 1109 52-53 0.6701907186415639 1109 54-55 -0.5194337372384581 1109 56-57 0.43861015860974817 1109 58-59 0.9015568875887041 1109 60-61 0.9563098091816755 1109 62-63 -0.46260155609817133 1109 64-65 1.9410334188572236 1109 66-67 0.7400714764888505 1109 68-69 1.5511357371803243 1109 70-71 1.9124407352129502 1109 72-73 2.624352487334985 1109 74-75 1.7414930682595902 1109 76 0.1663852608453098 1110 1 -2.1065656142262412 1110 2 -0.2919394061096696 1110 3 -0.318540580652801 1110 4 -0.7315046189919769 1110 5 0.33527585868957743 1110 6 -0.05852370726281464 1110 7 0.13947739653993096 1110 8 0.4870400451917831 1110 9 1.2695741196536972 1110 10-11 -1.267157061852771 1110 12-13 0.46158169557284623 1110 14-15 1.393164992470595 1110 16-17 0.6588457772480396 1110 18-19 -0.9379667765354469 1110 20-21 1.494929932122119 1110 22-23 -0.3389225069735602 1110 24-25 -1.823768161194245 1110 26-27 2.1939030994273594 1110 28-29 0.04814165123107017 1110 30-31 -1.250424666427783 1110 32-33 0.4241144815635849 1110 34-35 1.3928057428544207 1110 36-37 2.8812489215404824 1110 38-39 -1.2332103288643843 1110 40-41 -2.0485687634596346 1110 42-43 -0.2832041994856951 1110 44-45 1.222301947309873 1110 46-47 1.142736019165838 1110 48-49 2.4576584042963248 1110 50-51 -2.177704979448812 1110 52-53 0.9590420699929183 1110 54-55 -0.6875080615627809 1110 56-57 0.30389732077190956 1110 58-59 -2.3214160853842642 1110 60-61 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0.2721331786365653 2116 64-65 -2.6820897042658984 2116 66-67 -1.1718404354230643 2116 68-69 0.03411872016330619 2116 70-71 0.2858141085863224 2116 72-73 -0.9567285937460959 2116 74-75 1.3491006758671986 2116 76 2.3085274029874547 2117 1 1.1770882319276055 2117 2 0.6455605938903304 2117 3 0.039632496270279205 2117 4 -0.5896776959150571 2117 5 0.058833550997270834 2117 6 0.42945706196795186 2117 7 0.27649662730916447 2117 8 -0.7509407240389834 2117 9 -2.4972528034232226 2117 10-11 -1.5291762926219974 2117 12-13 -2.5023606121194604 2117 14-15 0.1864342232398215 2117 16-17 -1.5731253765981137 2117 18-19 -1.1146494688431403 2117 20-21 0.073054932122119 2117 22-23 -0.5492590454351003 2117 24-25 -2.160306622732705 2117 26-27 -4.746000746726487 2117 28-29 -0.645367964153543 2117 30-31 -0.17229966642778294 2117 32-33 -3.0506451338210283 2117 34-35 -1.5867615648378859 2117 36-37 -2.3074530015364374 2117 38-39 -0.04571032886438431 2117 40-41 -0.685587994228861 2117 42-43 0.2023727235912247 2117 44-45 -1.3125538219208934 2117 46-47 -1.2334659039110818 2117 48-49 -1.0062839033959818 2117 50-51 -1.0034261332949654 2117 52-53 0.4638497623006117 2117 54-55 -1.459142676947394 2117 56-57 -0.8799969099973239 2117 58-59 -3.0906468546150343 2117 60-61 -2.5192619163900467 2117 62-63 -0.823308416805034 2117 64-65 -2.338072610248801 2117 66-67 -3.5400740536566815 2117 68-69 -1.7144567784121918 2117 70-71 -1.6187442959720784 2117 72-73 -3.437497824515326 2117 74-75 -0.9913551645886436 2117 76 0.22875532321537406 2118 1 0.06170361654299228 2118 2 0.5686375169672502 2118 3 -1.768059811422031 2118 4 -0.01275461899197694 2118 5 -0.24885875669503577 2118 6 0.04484167735257216 2118 7 0.8534197042322376 2118 8 0.21059773749947652 2118 9 -1.8818681880386094 2118 10-11 -0.3945609080066177 2118 12-13 -0.00236061211946037 2118 14-15 1.0710496078552083 2118 16-17 -4.688509991982727 2118 18-19 -1.5569571611508337 2118 20-21 0.053824162891345395 2118 22-23 0.9315101853341332 2118 24-25 1.6473856849596018 2118 26-27 -2.726769977495721 2118 28-29 0.31617049738491687 2118 30-31 -0.710761204889323 2118 32-33 -0.3583374415133349 2118 34-35 -0.08676156483788589 2118 36-37 -3.788222232305671 2118 38-39 -2.4495564827105376 2118 40-41 1.064412005771139 2118 42-43 0.5869881082066115 2118 44-45 -3.90870766807474 2118 46-47 0.5934571730119913 2118 48-49 0.6475622504501644 2118 50-51 -0.6572722871411187 2118 52-53 -3.1899963915455416 2118 54-55 -3.0168349846397042 2118 56-57 -0.5915353715357838 2118 58-59 -2.0906468546150343 2118 60-61 -2.9038773010054335 2118 62-63 -2.3810007244973406 2118 64-65 0.4503889282127389 2118 66-67 0.2868490232663987 2118 68-69 -1.4836875476429618 2118 70-71 0.34279416556638154 2118 72-73 -0.03365167066917252 2118 74-75 -1.4336628568963334 2118 76 -1.3866292921692427 2119 1 1.285479840319212 2119 2 -0.127166678836943 2119 3 0.3718003284381055 2119 4 0.3508817446443899 2119 5 0.36652585868957743 2119 6 0.2965899291008185 2119 7 0.3639092147217511 2119 8 0.6091991361008766 2119 9 -0.5287213348917561 2119 10-11 0.42886566541995563 2119 12-13 -0.9673955771544271 2119 14-15 -0.9306986438930451 2119 16-17 -0.6692792227519604 2119 18-19 -0.1723417765354469 2119 20-21 -2.1542177951506076 2119 22-23 0.19374794757189306 2119 24-25 1.4375954751693953 2119 26-27 1.2137894630637263 2119 28-29 0.10638028759471041 2119 30-31 0.8958821517540301 2119 32-33 0.9056485724726784 2119 34-35 1.4482034701271544 2119 36-37 0.13835119426775577 2119 38-39 -3.022983056137111 2119 40-41 -1.113909672550541 2119 42-43 -2.016158744940242 2119 44-45 -0.27059577996285356 2119 46-47 -0.19675261719780224 2119 48-49 0.6650447679326845 2119 50-51 0.8322382023693748 2119 52-53 1.1806329790838248 2119 54-55 0.2698783020735789 2119 56-57 -2.4901367701371804 2119 58-59 -0.23050699447517076 2119 60-61 0.4212975241693897 2119 62-63 -1.1554762489728603 2119 64-65 -0.1947159668921543 2119 66-67 2.538597275014652 2119 68-69 2.9516271376717214 2119 70-71 0.6679689907412047 2119 72-73 -0.2976376846551858 2119 74-75 -0.1626838359173135 2119 76 0.609874204334254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 6.0 24 27.0 25 51.0 26 118.0 27 184.0 28 272.0 29 468.0 30 692.0 31 983.0 32 1369.0 33 1922.0 34 2607.0 35 2024.0 36 463.0 37 61.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8800782291759268 29.069250600053337 12.89003466974842 57.16063650102231 2 0.4000355587163304 3.9914659080807184 85.86541025868965 9.74308827451329 3 0.5778291403680328 33.70966308116277 62.9033691883723 2.8091385900968975 4 0.34669748422081964 45.05289359054138 25.122233087385542 29.478175837852255 5 0.4000355587163304 51.51569028358076 43.43497199751089 4.649302160192017 6 0.47115299137701133 47.56867277091297 8.276291225886744 43.68388301182327 7 0.8534091919281714 51.50680060449818 5.182682905147124 42.45710729842653 8 0.5156013867899368 46.81305004889323 28.962574451062316 23.70877411325451 9 0.6044981776157881 23.779891545915195 4.240376922393101 71.37523335407592 10-11 0.7245088452306871 25.895635167570454 42.75046670815183 30.629389279047025 12-13 14.694639523513201 3.5158680771624145 33.16739265712508 48.6220997421993 14-15 23.837674459951998 16.61481020535159 6.845052893590542 52.70246244110588 16-17 32.68734998666548 27.384656413903457 0.6845052893590542 39.243488310072 18-19 25.846741932616236 21.241888167837143 0.5600497822028625 52.35132011734377 20-21 17.12596675260023 10.618721664147925 0.7956262778913681 71.45968530536048 22-23 23.948795448484308 39.63463418970575 0.6622810916525913 35.754289270157344 24-25 32.72735354253712 44.590630278246955 1.1067650457818472 21.575251133434083 26-27 39.63018935016446 33.20739621299671 16.485909858654104 10.676504578184726 28-29 27.691350342252647 7.667348208729664 40.679171481909506 23.962129967108186 30-31 49.475508934127475 15.14356831718375 16.41034758645213 18.970575162236646 32-33 9.298604320384033 24.433282958485197 23.877678015823626 42.390434705307136 34-35 1.0978753666992622 48.63098942128189 16.579251489021246 33.6918837229976 36-37 1.0223130944972887 52.19130589385723 23.668770557382878 23.117610454262604 38-39 14.65463596764157 11.658814116810383 31.331673926571252 42.3548759889768 40-41 10.120899635523157 15.796959729753757 41.18143835007556 32.90070228464753 42-43 37.13218952795804 10.036447684238599 45.270690728064714 7.560672059738644 44-45 12.44999555516046 23.366521468574987 33.665214685749845 30.518268290514712 46-47 39.034580851631254 3.0180460485376477 21.228553649213264 36.718819450617836 48-49 29.047026402346876 1.1334340830296026 24.508845230687175 45.31069428393635 50-51 6.969508400746734 1.2045515156902835 48.550982309538625 43.27495777402436 52-53 14.530180460485376 1.1645479598186506 67.45488487865589 16.850386701040094 54-55 10.032002844697306 1.3556760600942306 49.475508934127475 39.13681216108099 56-57 37.0966308116277 1.6845941861498799 31.798382078406966 29.420392923815452 58-59 11.952173526535692 30.869410614276827 49.41328118054938 7.765134678638101 60-61 23.024268823895458 65.78807005067117 9.263045604053694 1.9246155213796783 62-63 2.649124366610365 94.76842385989866 0.8000711174326608 1.7823806560583164 64-65 0.44448395412925595 96.81305004889323 0.7378433638545648 2.004622633122944 66-67 0.413370077340208 94.39950217797137 0.8934127477998044 4.293714996888612 68-69 1.057871810827629 84.71864165703617 2.822473108720775 11.401013423415415 70-71 2.1646368566094765 74.79775980087119 5.5116010312027734 17.52600231131656 72-73 3.498088718997244 62.676682371766375 8.889679082585118 24.935549826651258 74-75 4.351497910925415 59.365276913503415 9.614187927815806 26.669037247755355 76 3.4936438794559517 62.281091652591336 8.667437105520492 25.557827362432217 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 1.0 21 2.0 22 1.5 23 0.5 24 0.0 25 0.0 26 0.0 27 0.5 28 1.0 29 1.0 30 1.5 31 2.0 32 5.0 33 8.0 34 6.0 35 3.5 36 7.0 37 11.0 38 36.5 39 220.0 40 1080.5 41 2260.0 42 2737.0 43 2672.0 44 2180.0 45 1346.5 46 940.0 47 724.0 48 364.0 49 166.5 50 113.0 51 82.0 52 35.5 53 13.0 54 6.0 55 7.0 56 7.5 57 8.0 58 9.0 59 5.0 60 1.5 61 2.0 62 2.0 63 1.5 64 1.0 65 1.0 66 1.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 11249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.632589563516756 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30037413148048 13.0233798559872 2 9.56707642971673 3.1825051115654723 3 2.61892036344201 1.3067828251400124 4 2.030999465526456 1.351231220552938 5 1.0689470871191877 0.8889679082585119 6 1.2292891501870657 1.2267757133967465 7 0.694815606627472 0.8089607965152459 8 0.5344735435595939 0.7111743266068095 9 0.5344735435595939 0.8000711174326608 >10 2.512025654730091 8.658547426437906 >50 0.427578834847675 4.818206062761134 >100 0.3206841261357563 11.796604142590454 >500 0.053447354355959376 6.293892790470264 >1k 0.10689470871191875 45.132900702284644 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 2860 25.424482176193443 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT 2217 19.708418526091208 Illumina PCR Primer Index 1 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 708 6.293892790470264 No Hit CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 359 3.1913947906480575 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 350 3.1113876789047916 No Hit TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 263 2.3379855987198863 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT 148 1.3156725042225976 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 104 0.9245266245888524 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 103 0.9156369455062673 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 90 0.8000711174326608 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 79 0.7022846475242244 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT 78 0.6933949684416393 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT 67 0.595608498533203 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT 60 0.5333807449551071 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 59 0.524491065872522 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT 58 0.5156013867899368 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 51 0.4533736332118411 No Hit TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 50 0.44448395412925595 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT 50 0.44448395412925595 No Hit CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT 43 0.3822562005511601 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC 38 0.3378078051382345 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 37 0.3289181260556494 No Hit CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG 37 0.3289181260556494 No Hit CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT 34 0.30224908880789403 Illumina PCR Primer Index 1 (95% over 21bp) CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT 32 0.2844697306427238 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG 31 0.27558005156013865 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT 28 0.24891101431238333 No Hit TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 25 0.22224197706462798 No Hit TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 25 0.22224197706462798 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTC 24 0.21335229798204286 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC 24 0.21335229798204286 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGT 23 0.20446261889945772 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCG 22 0.1955729398168726 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT 21 0.18668326073428748 Illumina PCR Primer Index 1 (95% over 22bp) ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT 21 0.18668326073428748 Illumina PCR Primer Index 1 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCG 20 0.17779358165170236 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTAGTATGCCGT 20 0.17779358165170236 No Hit ATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTGCTT 18 0.16001422348653213 Illumina PCR Primer Index 1 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT 18 0.16001422348653213 TruSeq Adapter, Index 4 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGT 18 0.16001422348653213 No Hit CTTTACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTC 17 0.15112454440394701 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGTCTT 17 0.15112454440394701 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT 17 0.15112454440394701 No Hit CTTATACACATCTCCGAGCCCAAGAGACTGGTCACAATCTCGTATGCCGT 16 0.1422348653213619 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT 15 0.13334518623877678 No Hit CTTATACACATCTCCGAGCACACGAGACTGGTCACAATCTCGTATGCCGT 14 0.12445550715619166 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATTTCGTATGCCGT 14 0.12445550715619166 No Hit ATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGTCTT 14 0.12445550715619166 Illumina PCR Primer Index 1 (95% over 22bp) ATACACATCTCCGCGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT 14 0.12445550715619166 Illumina PCR Primer Index 1 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGT 14 0.12445550715619166 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCACGTATGCCGT 14 0.12445550715619166 No Hit ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT 13 0.11556582807360655 TruSeq Adapter, Index 1 (95% over 22bp) CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC 13 0.11556582807360655 No Hit CTTATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGT 12 0.10667614899102143 No Hit CTTATACACATATCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 12 0.10667614899102143 No Hit ATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGTCTT 12 0.10667614899102143 TruSeq Adapter, Index 4 (95% over 21bp) CTTATACACATCTCCGAGCCCACGTGACTGGTCACAATCTCGTATGCCGT 12 0.10667614899102143 No Hit TCTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGC 12 0.10667614899102143 No Hit CTTCTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT 12 0.10667614899102143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008889679082585119 0.0 2 0.0 0.0 0.0 0.008889679082585119 0.0 3 0.0 0.0 0.0 0.008889679082585119 0.0 4 0.0 0.0 0.0 0.008889679082585119 0.0 5 0.0 0.0 0.0 0.008889679082585119 0.0 6 0.0 0.0 0.0 0.008889679082585119 0.0 7 0.0 0.0 0.0 0.008889679082585119 0.0 8 0.0 0.0 0.0 0.008889679082585119 0.0 9 0.0 0.0 0.0 0.008889679082585119 0.0 10 0.0 0.0 0.0 0.008889679082585119 0.0 11 0.0 0.0 0.0 0.008889679082585119 0.0 12 0.0 0.0 0.0 0.008889679082585119 0.0 13 0.0 0.0 0.0 0.008889679082585119 0.0 14 0.0 0.0 0.0 0.008889679082585119 0.0 15 0.0 0.0 0.0 0.008889679082585119 0.0 16 0.0 0.0 0.0 0.008889679082585119 0.0 17 0.0 0.0 0.0 0.008889679082585119 0.0 18 0.0 0.0 0.0 0.017779358165170237 0.0 19 0.0 0.0 0.0 0.026669037247755358 0.0 20 0.0 0.0 0.0 0.026669037247755358 0.0 21 0.0 0.0 0.0 0.035558716330340474 0.0 22 0.0 0.0 0.0 0.035558716330340474 0.0 23 0.0 0.0 0.0 0.035558716330340474 0.0 24 0.0 0.0 0.0 0.035558716330340474 0.0 25 0.0 0.0 0.0 0.04444839541292559 0.0 26 0.0 0.0 0.0 0.04444839541292559 0.0 27 0.0 0.0 0.0 0.053338074495510715 0.0 28 0.0 0.0 0.0 0.053338074495510715 0.0 29 0.0 0.0 0.0 0.06222775357809583 0.0 30 0.0 0.0 0.0 0.06222775357809583 0.0 31 0.0 0.0 0.0 0.06222775357809583 0.0 32 0.0 0.0 0.0 0.06222775357809583 0.0 33 0.0 0.0 0.0 0.06222775357809583 0.0 34 0.0 0.0 0.0 0.06222775357809583 0.0 35 0.0 0.0 0.0 0.10667614899102143 0.0 36 0.0 0.0 0.0 0.12445550715619166 0.0 37 0.0 0.0 0.0 0.12445550715619166 0.0 38 0.0 0.0 0.0 0.1422348653213619 0.0 39 0.0 0.0 0.0 0.15112454440394701 0.0 40 0.0 0.0 0.0 0.17779358165170236 0.0 41 0.0 0.0 0.0 0.17779358165170236 0.0 42 0.0 0.0 0.0 0.17779358165170236 0.0 43 0.0 0.0 0.0 0.20446261889945774 0.0 44 0.0 0.0 0.0 0.2311316561472131 0.0 45 0.0 0.0 0.0 0.2755800515601387 0.0 46 0.0 0.0 0.0 0.30224908880789403 0.0 47 0.0 0.0 0.0 0.3289181260556494 0.0 48 0.0 0.0 0.0 0.3378078051382345 0.0 49 0.0 0.0 0.0 0.37336652146857496 0.0 50 0.0 0.0 0.0 0.4267045959640857 0.0 51 0.0 0.0 0.0 0.4355942750466708 0.0 52 0.0 0.0 0.0 0.44448395412925595 0.0 53 0.0 0.0 0.0 0.49782202862476665 0.0 54 0.0 0.0 0.0 0.49782202862476665 0.0 55 0.0 0.0 0.0 0.5422704240376922 0.0 56 0.0 0.0 0.0 0.5422704240376922 0.0 57 0.0 0.0 0.0 0.5511601031202774 0.0 58 0.0 0.0 0.0 0.5778291403680327 0.0 59 0.0 0.0 0.0 0.5778291403680327 0.0 60 0.0 0.0 0.0 0.595608498533203 0.0 61 0.0 0.0 0.0 0.6044981776157881 0.0 62 0.0 0.0 0.0 0.6222775357809583 0.0 63 0.0 0.0 0.0 0.6400568939461285 0.0 64 0.0 0.0 0.0 0.6845052893590542 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTTAT 25 2.661529E-6 70.00001 1 GTCACAC 15 0.0021676668 70.0 28 AAAACTC 15 0.0021676668 70.0 70 CTTTACA 90 0.0 66.111115 1 TTTACAC 90 0.0 66.111115 2 CTATACA 50 1.0913936E-11 63.000004 1 CTTTATA 30 7.853239E-6 58.333332 2 TTTATAC 30 7.853239E-6 58.333332 3 CTTATAC 510 0.0 56.960785 1 TTACACA 105 0.0 56.666664 3 AAATATA 25 2.2899342E-4 56.000004 68 TTATACA 545 0.0 53.302753 2 ACAAAAT 20 0.0067745396 52.500004 67 GAGCACA 20 0.0067745396 52.500004 16 CGAGCAC 20 0.0067745396 52.500004 15 CCGAGCA 20 0.0067745396 52.500004 14 AGCACAC 20 0.0067745396 52.500004 17 GCACACG 20 0.0067745396 52.500004 18 GAAAAAC 30 5.632255E-4 46.666664 60 TATACAC 635 0.0 46.299213 3 >>END_MODULE