FastQCFastQC Report
Wed 25 May 2016
SRR1780367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82052
Sequences flagged as poor quality0
Sequence length50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1210.147467459659728No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG1120.1364988056354507No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC930.1133427582508653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC207.768288E-444.05198740
TCTACAC207.9098606E-443.8909233
TATACGC207.9098606E-443.8909233
TAAGGAT357.2400617E-637.6896931
ACATGTG357.3317005E-637.6207925
TTTACAC1350.037.388562
AGACGCT250.002312563135.2847513
TCAGACG250.002312563135.2847511
GACGATT250.0023405235.19853223
TACGCAT250.00236874435.1127365
CTTTACA1550.034.0423051
CTATACA607.867129E-932.9784771
TAAAATT401.8075923E-532.9784771
CTTATAC6300.032.4550131
TTATACA6100.032.0187842
TTACACA1600.031.54663
CACATGT353.238794E-431.3506584
ATGTGAC353.238794E-431.3506587
CAGTGTA1100.030.03544434
ATCAGAC300.00564733329.4039610
Produced by FastQC (version 0.11.5)