##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82052 Sequences flagged as poor quality 0 Sequence length 50 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.978440501145617 16.0 10.0 26.0 7.0 30.0 2 18.37968605274704 16.0 10.0 27.0 7.0 31.0 3 18.67637595671038 16.0 10.0 28.0 7.0 31.0 4 20.16257982742651 19.0 10.0 31.0 7.0 35.0 5 20.25633744454736 19.0 10.0 31.0 7.0 35.0 6 20.592867937405547 17.0 10.0 32.0 8.0 35.0 7 20.396321844683857 17.0 10.0 31.0 8.0 35.0 8 20.36034465948423 17.0 10.0 31.0 8.0 35.0 9 20.56048603324721 17.0 10.0 31.0 8.0 37.0 10 20.537634670696633 17.0 10.0 31.0 8.0 37.0 11 20.895029980987665 17.0 10.0 32.0 8.0 37.0 12 20.54994393799054 17.0 10.0 31.0 8.0 37.0 13 20.57386779115683 17.0 10.0 31.0 7.0 37.0 14 20.8172134743821 17.0 10.0 31.0 7.0 37.0 15 20.47947642958124 17.0 9.0 31.0 7.0 37.0 16 20.415943547993955 17.0 8.0 31.0 7.0 37.0 17 20.541814946619215 17.0 9.0 31.0 7.0 37.0 18 20.22989080095549 17.0 8.0 31.0 7.0 37.0 19 20.285404377711696 18.0 8.0 31.0 7.0 37.0 20 20.36470774630722 18.0 8.0 31.0 7.0 37.0 21 20.133244771608247 18.0 8.0 30.0 7.0 37.0 22 20.114573684980257 18.0 8.0 30.0 7.0 37.0 23 20.075391215326867 17.0 8.0 30.0 7.0 37.0 24 19.58782235655438 17.0 8.0 30.0 7.0 37.0 25 19.51074928094379 17.0 8.0 30.0 7.0 36.0 26 19.18477307073563 16.0 8.0 30.0 7.0 36.0 27 18.927801881733537 16.0 8.0 29.0 7.0 36.0 28 18.80926729391118 16.0 8.0 28.0 7.0 36.0 29 18.60398284015015 16.0 8.0 28.0 7.0 35.0 30 18.38879003558719 15.0 8.0 28.0 7.0 35.0 31 18.30970604007215 15.0 8.0 28.0 7.0 35.0 32 17.989543216496855 15.0 8.0 27.0 7.0 35.0 33 17.978379564178812 15.0 8.0 27.0 7.0 35.0 34 17.666223858041242 15.0 8.0 27.0 7.0 34.0 35 17.513625505776826 15.0 8.0 26.0 7.0 34.0 36 17.44346268220153 14.0 8.0 26.0 7.0 34.0 37 17.22346804465461 14.0 8.0 26.0 7.0 34.0 38 17.25193779554429 14.0 8.0 26.0 7.0 33.0 39 16.90744893482182 13.0 8.0 25.0 7.0 33.0 40 16.37387266611417 13.0 8.0 24.0 7.0 33.0 41 16.48065860673719 13.0 8.0 24.0 7.0 33.0 42 16.516940476770827 13.0 8.0 24.0 7.0 33.0 43 16.531492224443035 13.0 8.0 24.0 7.0 33.0 44 16.567140350021937 13.0 8.0 24.0 7.0 33.0 45 16.62824794033052 13.0 8.0 24.0 7.0 33.0 46 16.45562570077512 13.0 8.0 24.0 7.0 33.0 47 16.39398186515868 13.0 8.0 24.0 7.0 33.0 48 16.301321113440256 13.0 8.0 24.0 7.0 32.0 49 15.999110320284698 12.0 8.0 24.0 7.0 32.0 50 15.997135962560328 12.0 8.0 24.0 7.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5436501744415949 1101 2 -1.0842157461514468 1101 3 0.0024061035193021496 1101 4 -1.151983393574536 1101 5 -0.6072371201576985 1101 6 0.08280147392004267 1101 7 -1.5052162500098412 1101 8 -0.8051163672450521 1101 9 -0.5108118368457113 1101 10 -1.5398540308909112 1101 11 -0.1783554406202974 1101 12 -0.3073065581503194 1101 13 -1.6606762258576815 1101 14 -0.9855554146001388 1101 15 -0.2977358995571393 1101 16 -1.0202192128148582 1101 17 0.7136911456083581 1101 18 0.033963218672969475 1101 19 -0.6188406165793126 1101 20 -1.822513354487036 1101 21 -1.1368106503332456 1101 22 -1.2553667963524262 1101 23 -1.1784362058122753 1101 24 0.22187041614793301 1101 25 -0.9496639501639379 1101 26 -0.7939275130710755 1101 27 -0.596181750519694 1101 28 -1.252406150905177 1101 29 -0.8568904746545307 1101 30 -0.9866154524627895 1101 31 -0.4281851261109537 1101 32 -0.05961280441502126 1101 33 -0.30346286188795446 1101 34 -0.21075643853340154 1101 35 -0.4191472358849637 1101 36 0.05633964272640313 1101 37 -0.33895682588407894 1101 38 -0.9090746660450293 1101 39 -0.8072700852566967 1101 40 -2.2710957182220533 1101 41 -1.6444043194104587 1101 42 -1.5705575242131466 1101 43 -0.9081777206955266 1101 44 -1.0549136354737225 1101 45 -0.6733186248666989 1101 46 -1.5234105362754988 1101 47 -1.738625575492506 1101 48 -1.8790499317051523 1101 49 -1.4974136287226631 1101 50 -1.0662561199475729 1102 1 -0.29338492245220493 1102 2 -1.4668417408464087 1102 3 -0.6292915092127913 1102 4 -1.5472088577655185 1102 5 -1.7894652368685726 1102 6 -1.361628234302767 1102 7 -2.4756406531928654 1102 8 -1.5195726006668053 1102 9 -2.002721651169317 1102 10 -0.6576259141800378 1102 11 -1.5009018597184394 1102 12 -1.791391438786924 1102 13 -1.4393234407117923 1102 14 -1.366059393379981 1102 15 -2.4433592417322068 1102 16 -2.9235348626822315 1102 17 -1.0591205254791767 1102 18 -1.2456123781440063 1102 19 -1.4189732425740083 1102 20 -2.072115476502951 1102 21 -2.059887573410169 1102 22 -1.895817724734389 1102 23 -2.1850675055470212 1102 24 -2.144442581199545 1102 25 -2.449663950163938 1102 26 -1.6784102716917673 1102 27 -1.1205849335435651 1102 28 -1.910894214565655 1102 29 -2.1952193871213765 1102 30 -1.448684417980033 1102 31 -1.8665140385777974 1102 32 -3.5102759343884955 1102 33 -1.83489522263066 1102 34 -2.224814793971067 1102 35 -1.729094185487087 1102 36 -1.6276126119155077 1102 37 -1.2985058975021158 1102 38 -1.4886502628620057 1102 39 -2.5023629234529814 1102 40 -1.7871434635801435 1102 41 -1.7034228870497152 1102 42 -1.8661808663882127 1102 43 -2.520511938202157 1102 44 -1.5481497097442798 1102 45 -1.4861833463521101 1102 46 -1.2931718094850488 1102 47 -1.8912780953864043 1102 48 -1.5087581545168245 1102 49 -1.6536470504733263 1102 50 -1.8895982950138865 1103 1 -0.22351794732207253 1103 2 -0.05822404275502535 1103 3 -0.3107258643313564 1103 4 -0.006144658806583436 1103 5 0.020193525421767333 1103 6 0.47475117576085424 1103 7 0.9912188031402742 1103 8 -0.23634530165003298 1103 9 0.8237098121042514 1103 10 0.6409860002213499 1103 11 -0.2373391066820041 1103 12 0.6600255652616589 1103 13 -0.10901690514210216 1103 14 1.0375843313163742 1103 15 0.15079923137363593 1103 16 0.26102268503321113 1103 17 -0.06392098817437741 1103 18 -0.08841168410120659 1103 19 0.13932635256769288 1103 20 1.1680233320569116 1103 21 1.0887532594411908 1103 22 1.0795437558902492 1103 23 -0.14978699658230354 1103 24 -0.5517878674922017 1103 25 0.3322909370541076 1103 26 -0.4543501213158265 1103 27 0.24112703869472085 1103 28 0.4150167166084344 1103 29 0.08043127800297611 1103 30 0.5385336271327503 1103 31 -0.009660365934649917 1103 32 -1.015018560183755 1103 33 -0.13668816652886662 1103 34 -0.029268235266613374 1103 35 -0.7032410912129414 1103 36 -2.080359864662759 1103 37 -0.46710046083352097 1103 38 -0.5276901703229662 1103 39 -0.07373365798161302 1103 40 -0.48402027098326705 1103 41 -0.3164940264366436 1103 42 0.022714448822892308 1103 43 0.1727789814045515 1103 44 0.27105214103651676 1103 45 -1.3980399108402537 1103 46 -1.1072840130709363 1103 47 0.054413055567902546 1103 48 0.311253412863163 1103 49 0.6495918159234364 1103 50 -0.1401477455633362 1104 1 -0.24246065292328467 1104 2 -0.2971935595703208 1104 3 -0.13412156288005406 1104 4 -0.5212696806635506 1104 5 -0.19691902339212675 1104 6 -0.30539686876907624 1104 7 -0.9564993294003798 1104 8 -0.0406817241015105 1104 9 -0.06193453131243132 1104 10 -0.5664511974239232 1104 11 -0.3899298859557696 1104 12 -1.1429120112377298 1104 13 -0.47015230177321143 1104 14 -1.3781643426942338 1104 15 -0.3891553061329205 1104 16 -0.4003983093131218 1104 17 -0.3882201080671308 1104 18 -1.4624281205411442 1104 19 -0.4273811137725758 1104 20 -1.6895275218219723 1104 21 -0.7957253790154155 1104 22 -1.476497510065336 1104 23 -1.938794398808799 1104 24 -1.6685332192436704 1104 25 -0.7364856555902932 1104 26 -1.7295730623894414 1104 27 -1.9218967999717123 1104 28 -1.7179902193360768 1104 29 -2.0522259464535146 1104 30 -1.1316301544141396 1104 31 -0.6932880397704047 1104 32 -0.7709199415441326 1104 33 -1.0438397664589232 1104 34 -1.5168243348178194 1104 35 -1.2573589439844035 1104 36 0.4633832139640397 1104 37 -0.6565858020936481 1104 38 -1.064380733941313 1104 39 -1.3060003712764772 1104 40 -1.1006199096147284 1104 41 -0.5960883611105388 1104 42 -0.9933722796261364 1104 43 -1.800118378273714 1104 44 -1.9356273483847097 1104 45 -2.6482584805202674 1104 46 -1.168425238226849 1104 47 -1.5237766046291004 1104 48 -1.488245334004004 1104 49 -1.6364138960308683 1104 50 -1.4391510678224737 1105 1 -0.07314200747244826 1105 2 -0.013111260800137359 1105 3 -0.27313187936895034 1105 4 0.24949443893777712 1105 5 0.07282510436913725 1105 6 -0.3823916813820034 1105 7 0.06640677306508636 1105 8 -0.20627011368010528 1105 9 0.5831083083448512 1105 10 1.0545198348078166 1105 11 -0.5681661743511768 1105 12 0.7051383472165469 1105 13 1.6804567790684253 1105 14 0.9924715493614862 1105 15 0.7823781787420572 1105 16 0.4565114068377234 1105 17 0.8533722449083321 1105 18 0.7085807971017992 1105 19 0.07917597662784459 1105 20 1.6868203245381146 1105 21 1.3669487481629936 1105 22 1.2750324776947615 1105 23 -0.1798621845522277 1105 24 1.0873098768687015 1105 25 -0.9308669576827349 1105 26 0.30504837492477677 1105 27 0.22608944470976056 1105 28 -0.5549080954216379 1105 29 -0.04738827086920594 1105 30 -0.1381581021905589 1105 31 -0.0998859298444259 1105 32 0.6315979811696302 1105 33 0.36707123196737257 1105 34 -0.6608471826350346 1105 35 -0.8460982340700838 1105 36 -1.1104350526326847 1105 37 -0.3242433179763786 1105 38 0.17155794997778528 1105 39 -0.3519291467034158 1105 40 -0.01785485744943216 1105 41 -7.045527524347506E-4 1105 42 0.14301520070259244 1105 43 0.05247822952485137 1105 44 -0.2251884604672405 1105 45 0.09820069066350712 1105 46 -0.1599155920183044 1105 47 -0.8252861925523973 1105 48 0.26614063090827855 1105 49 0.34132113923170593 1105 50 -0.19277932451070434 1106 1 -0.14039346946075426 1106 2 0.12473335072868252 1106 3 0.45410287418159 1106 4 0.5987007881441251 1106 5 0.09747005006671472 1106 6 0.48770021502568284 1106 7 0.3725888950366816 1106 8 -0.2091940902882925 1106 9 -1.022990271437937 1106 10 1.1363911798370587 1106 11 0.3345071756905966 1106 12 0.348413201017717 1106 13 -0.9782733796617329 1106 14 -0.33626696358755126 1106 15 1.6198886215156598 1106 16 -0.08275342190079016 1106 17 0.04551927916062937 1106 18 -0.31606414859577825 1106 19 -0.7470562706570334 1106 20 1.1534034490159755 1106 21 0.8552528417302447 1106 22 0.5753666464499823 1106 23 1.5695112490317342 1106 24 0.29658305982609434 1106 25 2.137637637137651 1106 26 2.104129410847918 1106 27 0.9311855182268864 1106 28 1.8711570674856297 1106 29 1.1205315286295416 1106 30 0.675125105829494 1106 31 0.9381257660620079 1106 32 -0.03005615416871521 1106 33 0.10307791534247812 1106 34 0.6904477212914486 1106 35 1.1234088670159643 1106 36 1.8085290242261287 1106 37 1.0366589376627182 1106 38 0.5504217762100332 1106 39 -0.30138612247618113 1106 40 0.9449688685321895 1106 41 0.545661362034533 1106 42 1.4700828698755224 1106 43 1.2947505770603591 1106 44 1.9840847224901523 1106 45 0.8103978502290872 1106 46 1.72563160931837 1106 47 0.7803946762863667 1106 48 0.10699276123409263 1106 49 1.670895074068799 1106 50 1.4392173338017429 1107 1 -0.0813656916829757 1107 2 -0.859269625461792 1107 3 0.32972291386413133 1107 4 -1.0418002039193652 1107 5 0.0611357246698887 1107 6 0.05640061185107825 1107 7 -0.015183922423634755 1107 8 -0.6558706775898813 1107 9 0.3544898872922211 1107 10 -0.3879143757184984 1107 11 0.8087135248969446 1107 12 0.4063249073669226 1107 13 -0.18115036378871707 1107 14 0.5704790681584804 1107 15 0.05652056596010269 1107 16 -0.08002524729761618 1107 17 0.16816312836697733 1107 18 0.6845558910867631 1107 19 0.43907964204137784 1107 20 1.0818508696508964 1107 21 0.050689349666754424 1107 22 -0.652187917042081 1107 23 0.20976422522220872 1107 24 0.8382497264927622 1107 25 0.2847110498360621 1107 26 -0.09403527169176584 1107 27 0.029895835687202776 1107 28 -0.5298845991810381 1107 29 0.2492517667247789 1107 30 0.32319058201996853 1107 31 0.5295196152683559 1107 32 -0.02782401131157286 1107 33 -0.7247990687845061 1107 34 -1.1805840247402983 1107 35 -2.2157528393332413 1107 36 -1.3113866503770453 1107 37 -0.6921357051944241 1107 38 0.046205506368764304 1107 39 -0.44250715422221276 1107 40 -0.5361819251186049 1107 41 0.21604826679643807 1107 42 0.7290114413040953 1107 43 0.5877813310286122 1107 44 0.4677156748711049 1107 45 0.6871339613401979 1107 46 0.15358299820725918 1107 47 0.052592096921287634 1107 48 0.9779004993293299 1107 49 -0.5954739735502486 1107 50 -0.5988531027061939 1108 1 0.9219996929324097 1108 2 -0.305303279307946 1108 3 0.2860931061718226 1108 4 -0.26259347315013315 1108 5 -0.13561908302241932 1108 6 0.5845256118510775 1108 7 -0.5525637301159421 1108 8 -1.050341831436036 1108 9 -0.4334908819385497 1108 10 -0.865318221872343 1108 11 1.3144827556661767 1108 12 -0.2452375926330781 1108 13 -8.619022502571738E-4 1108 14 -0.9352901626107517 1108 15 -0.374128472501436 1108 16 -0.19276563191300156 1108 17 -1.036043602402252 1108 18 -0.6917662242978508 1108 19 -0.7497424733432361 1108 20 -0.3721154765029482 1108 21 -1.1675798811024762 1108 22 -0.895817724734389 1108 23 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2214 5 2.3989388963116802 2214 6 0.8173614327466012 2214 7 0.8685614133972592 2214 8 0.21842969554444736 2214 9 1.3073816783369985 2214 10 -0.38931362944984116 2214 11 -0.6819581168941014 2214 12 1.0771504670684138 2214 13 0.6943160541217317 2214 14 0.9492104114420634 2214 15 0.9300047077511486 2214 16 -0.012627859237912986 2214 17 1.5066659268744402 2214 18 1.1116780925793002 2214 19 1.1134033360712294 2214 20 0.5815010562180589 2214 21 0.4975177078757085 2214 22 0.7059044337041875 2214 23 0.777045008804297 2214 24 0.7393691294778364 2214 25 0.23690321401516812 2214 26 0.0036792805470398093 2214 27 0.8232727013588459 2214 28 1.7567292067891103 2214 29 2.142208856277019 2214 30 1.4348976715722088 2214 31 0.5933022645220873 2214 32 0.6650397946585755 2214 33 0.06671212524534553 2214 34 1.6309831394388077 2214 35 1.1707910039503417 2214 36 0.26079152126474625 2214 37 0.6326077649548303 2214 38 -0.10001650855661026 2214 39 0.5250768010016671 2214 40 -0.08002520870069318 2214 41 0.20415461008002111 2214 42 1.1534517398115582 2214 43 0.526330771327121 2214 44 -0.5336835788602379 2214 45 0.09315075238497528 2214 46 0.7786996026848705 2214 47 1.8353579551302417 2214 48 0.9346402381353016 2214 49 0.3919327428676631 2214 50 0.7116646211744033 2215 1 0.6415165397219837 2215 2 -0.5911265469493969 2215 3 0.543372190723634 2215 4 1.4206868828574954 2215 5 1.5406682659922026 2215 6 1.0952694135039707 2215 7 1.06752527179124 2215 8 1.5712729174514415 2215 9 1.1894588955566832 2215 10 1.1097612441162106 2215 11 0.42338294638454954 2215 12 0.38120868835865807 2215 13 1.2648723634840096 2215 14 1.0797765888196373 2215 15 0.5560686031501838 2215 16 0.930692727908994 2215 17 1.1881140581190444 2215 18 2.236015085301638 2215 19 1.21936115030584 2215 20 1.2847821713037888 2215 21 1.5408753000799784 2215 22 0.7308828474207303 2215 23 0.5663628822470024 2215 24 0.8186216273192102 2215 25 1.1040550581005242 2215 26 0.7976227861594722 2215 27 0.9593896373400952 2215 28 0.9234986652817732 2215 29 1.2016019733363485 2215 30 1.593464342350547 2215 31 0.8699767433675305 2215 32 0.9061894184404942 2215 33 0.8710170469179737 2215 34 0.5683829174084636 2215 35 1.0947456110799827 2215 36 0.4769010562345244 2215 37 0.3318183237311949 2215 38 -0.8061963531353662 2215 39 0.8528182590009266 2215 40 0.12239245504668617 2215 41 -0.11982466708785822 2215 42 1.3101064826264093 2215 43 0.17681800458233 2215 44 -0.10898887058344187 2215 45 -0.05782988163500846 2215 46 -0.8234960307183599 2215 47 -0.6295489154754073 2215 48 -0.08879629819132973 2215 49 -0.6287900892527283 2215 50 -0.1791087845243755 2216 1 -0.806540925327834 2216 2 0.2373279212671804 2216 3 -1.216494959967644 2216 4 -0.8092505543866544 2216 5 -0.9475529435544097 2216 6 -0.8491192479620047 2216 7 -1.0523480579376532 2216 8 -0.6422900701132441 2216 9 -1.4577180566330128 2216 10 -0.7088840018867231 2216 11 -0.8723612414581972 2216 12 -1.0620600225396188 2216 13 -0.0698769993027355 2216 14 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50 -0.1842064905566616 >>END_MODULE >>Per sequence quality scores fail #Quality Count 3 1.0 4 1.0 5 4.0 6 30.0 7 690.0 8 3177.0 9 6379.0 10 5784.0 11 4541.0 12 4155.0 13 4302.0 14 4340.0 15 4190.0 16 3955.0 17 3626.0 18 3345.0 19 2953.0 20 2670.0 21 2617.0 22 2298.0 23 2249.0 24 2159.0 25 2032.0 26 1897.0 27 1673.0 28 1586.0 29 1564.0 30 1422.0 31 1387.0 32 1204.0 33 1196.0 34 1091.0 35 1017.0 36 840.0 37 646.0 38 507.0 39 524.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.75496333174702 20.85148442361899 20.511037083135044 27.882515161498944 2 18.599095640303727 28.751812985240164 33.6551001255378 18.993991248918302 3 19.068628048408918 32.81617530560261 27.808314341080546 20.306882304907923 4 25.021937308048553 24.824501535611564 24.23585043630868 25.917710720031202 5 20.78803685467752 37.69682640276898 26.315019743577245 15.20011699897626 6 28.39053283283771 31.434943694242673 18.43465119680203 21.739872276117584 7 27.461853458782237 27.476478330814608 22.543021498561888 22.51864671184127 8 31.626285769999512 27.552040169648517 20.52966411543899 20.29200994491298 9 29.748208453176034 19.614390874079852 17.013601130990104 33.62379954175401 10 25.235216691853946 25.37537171549749 29.190025837273925 20.199385755374642 11 35.507184555946914 19.941256048067665 22.379983181192184 22.171576214793237 12 26.936342917037383 22.432936416044072 25.209326134383115 25.421394532535434 13 30.90507995319813 17.62382995319813 24.102964118564742 27.368125975039003 14 36.56562701866004 19.10977854156764 19.345009567686812 24.97958487208551 15 28.36788669908711 23.91921703413897 20.254244518385804 27.458651748388117 16 33.14904315563367 19.487160710798673 20.72349150556532 26.640304628002344 17 40.12505180020964 19.169734051629575 17.270799307705435 23.434414840455354 18 33.06560878852609 17.59169972535856 19.14311870613366 30.199572779981693 19 32.17721457173552 18.39413028616076 22.028518410000977 27.40013673210274 20 37.264410216321195 18.24744408750565 21.186285407174875 23.301860288998277 21 30.256165939824825 20.523814759164928 18.261443178070145 30.95857612294011 22 34.621340519185985 16.81318815309768 16.899759794178973 31.665711533537365 23 41.04139242357759 19.385939252108624 17.40529471990639 22.16737360440739 24 30.981722345591646 27.53361477830108 16.438907733229215 25.04575514287806 25 37.789726520111685 20.259214553080458 16.013753246278213 25.937305680529647 26 36.0610567896418 25.243227426787936 16.2956280022433 22.40008778132696 27 31.826826948867375 26.18687669161932 19.425276145424398 22.561020214088902 28 38.012045255047305 22.8103969569882 17.51316687798693 21.66439090997757 29 33.42924723841108 22.873027871930553 20.465019873686263 23.232705015972105 30 36.955143059248236 20.634436666097518 19.474595702124546 22.935824572529697 31 34.925435013230256 19.847821580558232 21.708592959309346 23.51815044690217 32 32.29047282303885 21.898851471628177 21.65622180496964 24.154453900363336 33 37.144877703806664 19.806374521423173 21.842612236935157 21.206135537835003 34 32.13188313905282 20.794517875432863 23.13685802077745 23.93674096473687 35 35.01383825698923 21.477950231044026 21.214596617856838 22.2936148941099 36 36.80282892330204 18.389220826728447 23.72637483233752 21.081575417631996 37 32.52462693845703 19.84541109919048 25.631522481225005 21.998439481127473 38 34.798883158369605 17.729251252789055 25.20331150858968 22.268554080251654 39 32.53304394478857 19.326440033165877 27.124079403014196 21.01643661903136 40 26.549871810523744 31.12928824319375 23.486753754120375 18.83408619216213 41 31.571054075474 16.11046759739072 27.43278668536243 24.885691641772848 42 25.484362616594524 19.391574711942937 33.015911723465216 22.108150947997316 43 25.622706380073396 16.506748271784055 31.167627802635913 26.702917545506637 44 26.168417973035567 15.925104225077408 33.98956530219178 23.916912499695247 45 26.37180440586636 18.011142672534653 33.704755751155105 21.91229717044388 46 25.753918915624467 18.903434993539577 29.35225139569468 25.990394695141276 47 31.105476797054987 19.69599083340444 28.617574996647853 20.58095737289272 48 30.932471964895175 20.802047781569964 25.13651877133106 23.128961482203803 49 28.38371526084837 22.313505607020964 26.327401267674304 22.975377864456362 50 30.159446083331304 22.03232805909745 24.271643464904795 23.536582392666457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 4.0 10 8.0 11 6.5 12 5.0 13 5.5 14 6.0 15 12.5 16 19.0 17 23.0 18 27.0 19 38.5 20 50.0 21 69.0 22 88.0 23 132.0 24 176.0 25 212.0 26 248.0 27 303.0 28 358.0 29 453.5 30 549.0 31 669.0 32 789.0 33 985.5 34 1182.0 35 1377.5 36 1573.0 37 1816.5 38 2060.0 39 2238.0 40 2416.0 41 2788.0 42 3160.0 43 3345.5 44 3531.0 45 3862.0 46 4193.0 47 4501.0 48 4809.0 49 5133.0 50 5457.0 51 5667.0 52 5877.0 53 6115.5 54 6354.0 55 6074.5 56 5795.0 57 5624.5 58 5454.0 59 5025.0 60 4596.0 61 4321.0 62 4046.0 63 3730.0 64 3414.0 65 3147.0 66 2880.0 67 2669.5 68 2459.0 69 2345.0 70 2231.0 71 2014.5 72 1798.0 73 1698.0 74 1598.0 75 1483.5 76 1369.0 77 1201.0 78 1033.0 79 937.5 80 842.0 81 707.0 82 572.0 83 485.5 84 399.0 85 326.0 86 253.0 87 215.5 88 178.0 89 137.0 90 96.0 91 67.5 92 39.0 93 33.5 94 28.0 95 21.5 96 15.0 97 11.0 98 7.0 99 10.5 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12309267293911179 2 0.0060936966801540485 3 0.0012187393360308097 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0012187393360308097 12 0.003656218008092429 13 0.004874957344123239 14 0.0060936966801540485 15 0.0060936966801540485 16 0.1413737629795739 17 0.007312436016184858 18 0.15477989567591283 19 0.17062350704431337 20 0.22302929849363817 21 0.23277921318188466 22 0.04753083410520158 23 0.009749914688246477 24 0.11456149758689613 25 0.04265587676107834 26 0.036562180080924295 27 0.036562180080924295 28 0.03412470140886267 29 0.04143713742504753 30 0.07068688148978695 31 0.052405791449324814 32 0.04143713742504753 33 0.04631209476917077 34 0.04874957344123239 35 0.04021839808901673 36 0.05118705211329401 37 0.03412470140886267 38 0.04265587676107834 39 0.04874957344123239 40 0.173060985716375 41 0.04509335543313996 42 0.04509335543313996 43 0.0377809194169551 44 0.021937308048554576 45 0.03046848340077024 46 0.017062350704431337 47 0.018281090040462147 48 0.014624872032369717 49 0.014624872032369717 50 0.021937308048554576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 82052.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.36815677862819 #Duplication Level Percentage of deduplicated Percentage of total 1 98.78085795224607 95.1932920586945 2 0.7512140833670579 1.447862331204602 3 0.18717118575475514 0.5411202651976795 4 0.07588021044111695 0.29249744064739436 5 0.04046944556859571 0.19499829376492955 6 0.03035208417644678 0.1754984643884366 7 0.022764063132335085 0.15356115633988204 8 0.024028733306353703 0.18524837907668307 9 0.018970052610279238 0.1645298103641593 >10 0.058174828004856335 0.8628674499098133 >50 0.007588021044111695 0.5045580851167553 >100 0.002529340348037232 0.28396626529517865 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 121 0.147467459659728 No Hit CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG 112 0.1364988056354507 No Hit ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC 93 0.1133427582508653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.018281090040462147 0.0 2 0.0 0.0 0.0 0.018281090040462147 0.0 3 0.0 0.0 0.0 0.018281090040462147 0.0 4 0.0 0.0 0.0 0.029249744064739434 0.0 5 0.0 0.0 0.0 0.035343440744893484 0.0 6 0.0 0.0 0.0 0.035343440744893484 0.0 7 0.0 0.0 0.0 0.035343440744893484 0.0 8 0.0 0.0 0.0 0.0377809194169551 0.0 9 0.0 0.0 0.0 0.03899965875298591 0.0 10 0.0 0.0 0.0 0.03899965875298591 0.0 11 0.0 0.0 0.0 0.03899965875298591 0.0 12 0.0 0.0 0.0 0.04021839808901672 0.0 13 0.0 0.0 0.0 0.04143713742504753 0.0 14 0.0 0.0 0.0 0.04143713742504753 0.0 15 0.0 0.0 0.0 0.04265587676107834 0.0 16 0.0 0.0 0.0 0.04265587676107834 0.0 17 0.0 0.0 0.0 0.04265587676107834 0.0 18 0.0 0.0 0.0 0.05849948812947887 0.0 19 0.0 0.0 0.0 0.0633744454736021 0.0 20 0.0 0.0 0.0 0.06459318480963291 0.0 21 0.0 0.0 0.0 0.07068688148978697 0.0 22 0.0 0.0 0.0 0.0865304928581875 0.0 23 0.0 0.0 0.0 0.09140545020231072 0.0 24 0.0 0.0 0.0 0.09262418953834153 0.0 25 0.0 0.0 0.0 0.09262418953834153 0.0 26 0.0 0.0 0.0 0.09506166821040316 0.0 27 0.0 0.0 0.0 0.10603032223468045 0.0 28 0.0 0.0 0.0 0.11943645493101936 0.0 29 0.0 0.0 0.0 0.14502998098766637 0.0 30 0.0 0.0 0.0 0.16574854970019012 0.0 31 0.0 0.0 0.0 0.1901233364208063 0.0 32 0.0 0.0 0.0 0.21449812314142253 0.0 33 0.0 0.0 0.0 0.2632476965826549 0.0 34 0.0 0.0012187393360308097 0.0 0.3034660946716716 0.0 35 0.0 0.0012187393360308097 0.0 0.3607468434651197 0.0 36 0.0 0.0012187393360308097 0.0 0.4753083410520158 0.0 37 0.0 0.0012187393360308097 0.0 0.6130258860234973 0.0 38 0.0 0.0012187393360308097 0.0 0.7678057816994102 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCTC 20 7.768288E-4 44.051987 40 TCTACAC 20 7.9098606E-4 43.890923 3 TATACGC 20 7.9098606E-4 43.890923 3 TAAGGAT 35 7.2400617E-6 37.689693 1 ACATGTG 35 7.3317005E-6 37.620792 5 TTTACAC 135 0.0 37.38856 2 AGACGCT 25 0.0023125631 35.28475 13 TCAGACG 25 0.0023125631 35.28475 11 GACGATT 25 0.00234052 35.198532 23 TACGCAT 25 0.002368744 35.112736 5 CTTTACA 155 0.0 34.042305 1 CTATACA 60 7.867129E-9 32.978477 1 TAAAATT 40 1.8075923E-5 32.978477 1 CTTATAC 630 0.0 32.455013 1 TTATACA 610 0.0 32.018784 2 TTACACA 160 0.0 31.5466 3 CACATGT 35 3.238794E-4 31.350658 4 ATGTGAC 35 3.238794E-4 31.350658 7 CAGTGTA 110 0.0 30.035444 34 ATCAGAC 30 0.005647333 29.40396 10 >>END_MODULE