FastQCFastQC Report
Wed 25 May 2016
SRR1780367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82052
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3026236.881489786964366No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT2166426.402768975771462RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC63337.718276215083118No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG42645.196704528835372No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC34814.242431628723248No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT21592.631258226490518No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC12441.5161117340223274No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC10991.33939453029786No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG7210.8787110612782139No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC6530.7958367864281187No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC5820.7093062935699312No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5340.6508068054404524No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC4500.5484327012138644No Hit
CTTATCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC3820.4655584263637693No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3460.42168381026666013No Hit
ACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCT2920.3558718861209964RNA PCR Primer, Index 32 (95% over 23bp)
CTTATTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG2720.33149709940038025No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC2560.3119972700238873No Hit
ATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCTGCTT2420.294934919319456TruSeq Adapter, Index 8 (96% over 26bp)
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT2420.294934919319456No Hit
CTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT2340.2851850046312095No Hit
TCTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT1900.23156047384585385No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTCTTC1690.20596694778920685TruSeq Adapter, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT1390.16940476770828256No Hit
CTTATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC1300.15843611368400526No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGTCTT1220.14868619899575877No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGC1060.12918636961926583No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG1010.12309267293911179No Hit
CTTATACCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG840.10237410422658802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC207.7313685E-444.0946851
CTTATCA454.5838533E-1044.0946851
CTTATAA850.044.0946851
TATAACA800.044.0140273
TTATAAC800.044.0140272
ATAACAC750.044.0140234
TCTGCTT408.172719E-943.98720644
CTTTACA5900.042.599951
TTTACAC5950.042.1646962
TCTTTAT1000.041.889951
CCGTCTT24500.041.74295844
CTTAACA750.041.1550371
TTAACAC750.041.0797582
TTATCAC501.3114914E-939.612622
CTATACA3550.039.126271
TTCTGCT452.3115717E-839.0997443
CTTCTGC452.3115717E-839.0997442
GCCGTCT26800.037.99640743
CTCTTAT1400.037.7954441
CTTATAC38450.037.099171
Produced by FastQC (version 0.11.5)