FastQCFastQC Report
Wed 25 May 2016
SRR1780366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52318
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT150.002213496569.9961846
TGGATAA150.002213496569.9961868
GCGTAGC150.002213496569.9961824
AGGGTTG252.3705636E-455.9969445
AGACTAT200.00691667252.4971370
TTCAATG200.00691667252.4971332
GGTTTAA200.00691667252.4971338
TTGAGGA200.00691667252.497132
GTAGCTG200.00691667252.4971326
TCAATGA200.00691667252.4971333
GATGGAT200.00691667252.4971366
CGTAGCT200.00691667252.4971325
TGCTATG200.00691667252.4971360
TAGCTGG200.00691667252.4971327
TGATGGA200.00691667252.4971365
TTTACAC404.536097E-543.747612
ATTAGAC350.001245147139.99781467
TAGATCT350.001245147139.99781439
TCTTTAA400.002399094634.9980923
GCTGGGT500.00715050327.99847229
Produced by FastQC (version 0.11.5)