##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780366_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52318 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.685825146221188 34.0 23.0 34.0 12.0 34.0 2 28.65120990863565 34.0 23.0 34.0 11.0 34.0 3 28.863125501739365 34.0 23.0 34.0 11.0 34.0 4 29.162984059023664 34.0 27.0 34.0 11.0 34.0 5 29.164341144539165 34.0 27.0 34.0 11.0 34.0 6 31.577124507817576 37.0 27.0 38.0 11.0 38.0 7 30.88032799418938 36.0 26.0 38.0 11.0 38.0 8 30.36757903589587 36.0 26.0 38.0 11.0 38.0 9 30.896555678733897 36.0 26.0 38.0 12.0 38.0 10-11 30.953170992774954 37.0 26.5 38.0 11.0 38.0 12-13 31.236476929546235 37.0 26.5 38.0 11.0 38.0 14-15 30.904382812798655 37.0 26.0 38.0 11.0 38.0 16-17 30.70907718184946 37.0 26.0 38.0 11.0 38.0 18-19 30.754759356244506 37.0 25.5 38.0 11.0 38.0 20-21 30.803958484651552 37.0 26.0 38.0 11.0 38.0 22-23 30.689246530830687 37.0 26.0 38.0 11.0 38.0 24-25 31.027485760159028 37.0 26.0 38.0 11.0 38.0 26-27 31.035838525937535 37.0 26.0 38.0 11.0 38.0 28-29 31.016179899843266 37.0 26.0 38.0 11.0 38.0 30-31 30.88037577889063 37.0 26.0 38.0 11.0 38.0 32-33 30.756240681983257 37.0 26.0 38.0 11.0 38.0 34-35 30.717773997476968 37.0 26.0 38.0 11.0 38.0 36-37 30.624335792652623 37.0 26.0 38.0 11.0 38.0 38-39 30.88949309988914 37.0 26.0 38.0 11.0 38.0 40-41 30.60961810466761 37.0 25.5 38.0 11.0 38.0 42-43 30.88356779693413 37.0 26.0 38.0 11.0 38.0 44-45 30.11931839902137 36.0 24.0 38.0 11.0 38.0 46-47 30.73838831759624 37.0 26.0 38.0 11.0 38.0 48-49 30.56912534882832 36.5 26.0 38.0 11.0 38.0 50-51 30.51852135020452 36.5 25.5 38.0 11.0 38.0 52-53 30.53817997629879 37.0 25.5 38.0 11.0 38.0 54-55 30.348101991666347 36.0 25.0 38.0 11.0 38.0 56-57 30.26820597117627 36.5 24.5 38.0 11.0 38.0 58-59 30.17345846553767 36.5 24.5 38.0 11.0 38.0 60-61 30.255495240643754 36.0 24.0 38.0 11.0 38.0 62-63 30.23742306663099 36.0 24.5 38.0 11.0 38.0 64-65 30.29728774035705 36.0 24.0 38.0 11.0 38.0 66-67 30.2999350128063 36.0 24.5 38.0 11.0 38.0 68-69 29.835907335907336 36.0 23.5 38.0 11.0 38.0 70-71 29.793952368209794 36.0 23.5 38.0 11.0 38.0 72-73 29.83731220612409 36.0 24.0 38.0 11.0 38.0 74-75 29.576962039833326 35.5 23.0 38.0 11.0 38.0 76 28.823559769104325 34.0 22.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.5322403182589142 1101 2 -0.30859725098116186 1101 3 0.6031120704285584 1101 4 -1.8320551286863918 1101 5 -1.443421746004347 1101 6 -2.3195359543145777 1101 7 -0.6901989674045304 1101 8 -1.2971741330690811 1101 9 -0.4321223854348837 1101 10-11 -0.46860161319949967 1101 12-13 -1.3373021911554943 1101 14-15 -0.6002658674533201 1101 16-17 -2.1433112508689796 1101 18-19 -0.8309108848806588 1101 20-21 -0.7561276828743573 1101 22-23 -1.1356394136183958 1101 24-25 -0.9875815749890826 1101 26-27 -1.3109997158626285 1101 28-29 -0.29692575142619404 1101 30-31 -1.4488091041024624 1101 32-33 -0.05265780983253521 1101 34-35 -1.2347166526275473 1101 36-37 -0.7700519212845123 1101 38-39 -0.9703969170876228 1101 40-41 -0.07161615614446148 1101 42-43 -0.5093708264703665 1101 44-45 0.012645595491004968 1101 46-47 -0.15497806404299297 1101 48-49 -1.3783093108717175 1101 50-51 -1.17432906353746 1101 52-53 -0.6822085957411907 1101 54-55 -1.2987773973648302 1101 56-57 -0.9440737299305901 1101 58-59 -1.1869649740931436 1101 60-61 -0.5608726051524329 1101 62-63 -0.6974846264595804 1101 64-65 -0.8824008811218853 1101 66-67 -1.459571981113843 1101 68-69 -0.5895012750839719 1101 70-71 -1.1811792431259036 1101 72-73 -0.5501111137816785 1101 74-75 -1.342301064758427 1101 76 -1.0303256292562821 1102 1 0.2772002411816459 1102 2 -0.026429418813329164 1102 3 1.1559092732257596 1102 4 0.3177117710805071 1102 5 0.9205642679816677 1102 6 0.42859924382062076 1102 7 2.472504995299431 1102 8 1.5178608319658835 1102 9 1.591770388457892 1102 10-11 0.3492305546326726 1102 12-13 -2.4245982284515364 1102 14-15 -0.7961283383157891 1102 16-17 -2.0195933021510264 1102 18-19 -0.249500628470404 1102 20-21 0.31613338938671376 1102 22-23 -1.5344739124528992 1102 24-25 0.6429545555470462 1102 26-27 -3.4914775713404858 1102 28-29 0.8285987240982813 1102 30-31 1.083417003123646 1102 32-33 -5.747529604704329 1102 34-35 -0.6171758600867534 1102 36-37 0.4541905029579141 1102 38-39 0.41142126473055995 1102 40-41 1.1538500443217394 1102 42-43 1.790512623413079 1102 44-45 -4.39020988236447 1102 46-47 0.24187508281015369 1102 48-49 0.6488468662844582 1102 50-51 -5.744142583350978 1102 52-53 -1.5122202507528435 1102 54-55 0.7853135117260734 1102 56-57 -0.694423380280238 1102 58-59 1.3950863079581346 1102 60-61 0.495013175733348 1102 62-63 -3.5165988455737995 1102 64-65 0.3438811701601665 1102 66-67 0.02912265758079613 1102 68-69 -5.10162248720518 1102 70-71 -0.16964078158743945 1102 72-73 0.506532242861681 1102 74-75 -5.508676356133723 1102 76 -1.468554067484721 1103 1 -0.06869547384158636 1103 2 -0.4884325163868972 1103 3 -0.4595785946111022 1103 4 0.46329772874700126 1103 5 0.12567526436783183 1103 6 -2.112753363303799 1103 7 0.046123993750647685 1103 8 1.4235397168394002 1103 9 -1.5421996683309587 1103 10-11 0.45787794750825483 1103 12-13 -1.290576545436565 1103 14-15 -0.5000777446141882 1103 16-17 0.40898181400797995 1103 18-19 -0.16284600792316795 1103 20-21 -0.08900342011251183 1103 22-23 -0.8842157813222826 1103 24-25 0.21213369855169262 1103 26-27 -0.041348505775179234 1103 28-29 0.30908401062383817 1103 30-31 0.015218758415333156 1103 32-33 -3.991695841152442 1103 34-35 -0.9403044093898032 1103 36-37 -0.5421182218743006 1103 38-39 0.6845756271466641 1103 40-41 0.25462443771358423 1103 42-43 0.48310425996444906 1103 44-45 -3.662486598936489 1103 46-47 -0.30445945513512385 1103 48-49 -0.368293040788334 1103 50-51 0.18401952299904778 1103 52-53 -0.4613167917956922 1103 54-55 0.28900478689386233 1103 56-57 0.5098615964879585 1103 58-59 -0.13439226715802377 1103 60-61 1.2394375433120786 1103 62-63 0.28487250187070146 1103 64-65 -0.23335166411964536 1103 66-67 -0.3287503419029427 1103 68-69 -2.8720922858629017 1103 70-71 0.04700867633718531 1103 72-73 0.9996143285612185 1103 74-75 -1.215826588451737 1103 76 1.051838291833814 1104 1 -0.7532592322308496 1104 2 -0.3542043284674321 1104 3 -0.6474980576983498 1104 4 -0.1541519356825276 1104 5 -0.05553278932344341 1104 6 0.6456358984411708 1104 7 1.5360568796566838 1104 8 1.2893115289199386 1104 9 0.9007533518032673 1104 10-11 -0.04212205249174872 1104 12-13 -0.9650731897318643 1104 14-15 -0.6645072748155307 1104 16-17 -0.7084678504215525 1104 18-19 -0.06888627637954059 1104 20-21 0.6794529557264184 1104 22-23 0.44464327908040246 1104 24-25 -0.7308193215825334 1104 26-27 0.3311347157013351 1104 28-29 0.6614330039124248 1104 30-31 -0.5686738590343303 1104 32-33 0.05192831992138025 1104 34-35 -0.31278763086631045 1104 36-37 -0.6629235910018174 1104 38-39 -0.9261626278868924 1104 40-41 -0.08765744148104915 1104 42-43 0.5703525821121005 1104 44-45 -2.4611443170572933 1104 46-47 -0.13331851553780893 1104 48-49 -0.02265545689571624 1104 50-51 -0.2992019535110195 1104 52-53 -0.4881624293795852 1104 54-55 0.13464237078648011 1104 56-57 -0.3995343766664057 1104 58-59 0.32869498116412643 1104 60-61 0.2193033151241579 1104 62-63 -0.21177179343131058 1104 64-65 0.10221880567900854 1104 66-67 -0.27841477143314464 1104 68-69 -3.3150453059971277 1104 70-71 -1.3925886390990598 1104 72-73 0.06672842252094924 1104 74-75 -0.041329944156434095 1104 76 -0.8139335202467244 1105 1 -0.9663895024080986 1105 2 -0.8482242906082007 1105 3 -0.6492189319024462 1105 4 -0.3553651519964163 1105 5 -0.10892291150551259 1105 6 -0.37781101258963545 1105 7 -0.18903346623902806 1105 8 -0.2872673731623223 1105 9 0.09818064486814393 1105 10-11 0.22487158027369603 1105 12-13 -1.4515213053746123 1105 14-15 -0.994205261392711 1105 16-17 0.33745797990025395 1105 18-19 -0.27642370539347993 1105 20-21 -0.4947640465107206 1105 22-23 -0.006268784247769332 1105 24-25 -0.42755826496577143 1105 26-27 -0.5639134687763807 1105 28-29 -0.7508884553888997 1105 30-31 -0.5403009455943035 1105 32-33 0.569137061962337 1105 34-35 0.7309010629901707 1105 36-37 1.1349597337271433 1105 38-39 0.1505238288331192 1105 40-41 -0.32948328901159485 1105 42-43 0.6103844182848803 1105 44-45 1.470687553532965 1105 46-47 0.895721236656307 1105 48-49 0.12576994320753343 1105 50-51 0.17124203203363564 1105 52-53 -0.08080999434258729 1105 54-55 -0.6941736677611061 1105 56-57 0.46006379920693874 1105 58-59 -0.6517085638367348 1105 60-61 -0.33447400375383296 1105 62-63 -0.3268552558302069 1105 64-65 -0.5330419067629109 1105 66-67 0.05412265758079471 1105 68-69 0.3682493076666127 1105 70-71 1.0880515261048664 1105 72-73 -0.11974980842037297 1105 74-75 -0.37085584331320476 1105 76 -0.9685540674847211 1106 1 -0.49146491718939345 1106 2 0.12990542281563222 1106 3 -0.15958846433080254 1106 4 -0.1551389076525247 1106 5 -0.4701597139187861 1106 6 -0.7350885389848081 1106 7 -0.023309484338575714 1106 8 0.47555313965819224 1106 9 -0.09544680611224621 1106 10-11 -0.5321042869962902 1106 12-13 0.2891461154398698 1106 14-15 -0.284646437863298 1106 16-17 -0.11880144694740835 1106 18-19 0.09433421315855739 1106 20-21 -0.6332444386675817 1106 22-23 0.6268383047416748 1106 24-25 -0.17463594219050904 1106 26-27 0.14064912549209296 1106 28-29 -1.106751954634749 1106 30-31 -0.7310060737994313 1106 32-33 -0.5073146725776319 1106 34-35 -0.5108975795437694 1106 36-37 -0.7465447911597316 1106 38-39 -0.32901991174002987 1106 40-41 -0.42912506880043466 1106 42-43 -0.18561859830637317 1106 44-45 0.17986930315588978 1106 46-47 0.05692033167893129 1106 48-49 -0.09934317896440703 1106 50-51 -0.6290973344822 1106 52-53 -0.22777454939537733 1106 54-55 -0.06983354709745271 1106 56-57 0.10438883691432821 1106 58-59 -1.2813277191911858 1106 60-61 0.5271398725659253 1106 62-63 -0.9109427369765157 1106 64-65 -0.28926362621992396 1106 66-67 -0.43795879038300356 1106 68-69 0.08440692455952359 1106 70-71 -0.4164733607729616 1106 72-73 -0.6547234132469164 1106 74-75 -1.7889365371291923 1106 76 -0.7219477326430912 1107 1 -0.21166615557948631 1107 2 -0.3271379208376217 1107 3 0.4541886254524812 1107 4 -0.47479835037698237 1107 5 0.03564523964158539 1107 6 -0.2509554120498265 1107 7 -1.0685881221094746 1107 8 -0.2283942287628591 1107 9 -0.6759421621493971 1107 10-11 -0.6671662065009336 1107 12-13 0.1297033909816676 1107 14-15 -0.3297315771821836 1107 16-17 0.1538378719380411 1107 18-19 0.9070277116105672 1107 20-21 -0.025465800896682822 1107 22-23 -0.8660022525338711 1107 24-25 -1.561245849311252 1107 26-27 -0.7970443729599204 1107 28-29 -1.0875956078855253 1107 30-31 -0.8395587053783764 1107 32-33 0.4955878042025539 1107 34-35 -0.17421027304222036 1107 36-37 -0.32278318125261407 1107 38-39 -0.06489452474312785 1107 40-41 0.2961071293419799 1107 42-43 0.08702072057907628 1107 44-45 -0.012274659692405265 1107 46-47 -0.29253787265543707 1107 48-49 -0.16648714181270563 1107 50-51 0.37999709276238036 1107 52-53 -0.17881944103624292 1107 54-55 -0.36994964616866355 1107 56-57 0.6411535427966832 1107 58-59 0.24943853467878085 1107 60-61 -0.571737836412403 1107 62-63 -0.7379551208774409 1107 64-65 -0.3111289512973201 1107 66-67 -0.10301296994957099 1107 68-69 -0.22596459246834044 1107 70-71 -0.3686792431259036 1107 72-73 -0.2450871903367009 1107 74-75 -1.060801862206592 1107 76 -1.1836350391446402 1108 1 -2.055598904117499 1108 2 -1.0106174530013625 1108 3 -2.0648172225007357 1108 4 -0.3099591690904333 1108 5 -0.1650126550952571 1108 6 -0.7399904997691245 1108 7 0.36598790128233816 1108 8 1.1769420285470815 1108 9 -1.283763799576299 1108 10-11 -0.22838696673484904 1108 12-13 -0.6734764335797365 1108 14-15 -1.0983719280593789 1108 16-17 -0.8759501397578653 1108 18-19 -0.8239664404362159 1108 20-21 -1.2933751576218313 1108 22-23 -2.0030636560426416 1108 24-25 -1.3439578376153456 1108 26-27 -1.4514668875798016 1108 28-29 -1.7824055494059934 1108 30-31 -1.0782282960216527 1108 32-33 -1.3859911431658674 1108 34-35 -1.6769515011123985 1108 36-37 -1.5194206081531973 1108 38-39 -2.146217624158332 1108 40-41 -1.2165025197808248 1108 42-43 -1.50526729111683 1108 44-45 -0.6836927883473756 1108 46-47 -1.1136270539419826 1108 48-49 -1.1573497149121224 1108 50-51 -1.761450275658671 1108 52-53 -1.0300621310947236 1108 54-55 -1.6605198216072559 1108 56-57 -0.844957568314431 1108 58-59 -1.7911316407598115 1108 60-61 -0.9677539182837478 1108 62-63 -2.000199272924224 1108 64-65 -2.1532342144552175 1108 66-67 -1.1761123851542443 1108 68-69 -0.7113447094274044 1108 70-71 -0.09437368757034648 1108 72-73 -0.6798459622665263 1108 74-75 -0.45499045869782506 1108 76 -1.9551993666300191 1109 1 -1.0394250689920526 1109 2 -0.15273214834186533 1109 3 0.0551648563523095 1109 4 -0.6023874845026178 1109 5 0.5297454094208724 1109 6 0.5065148765749612 1109 7 -1.44982751090404 1109 8 0.8868434622388364 1109 9 1.318073117986426 1109 10-11 0.2835978002902415 1109 12-13 0.3595208782482189 1109 14-15 0.388859254736321 1109 16-17 0.1956672437546807 1109 18-19 0.19355148071073813 1109 20-21 0.26464455563981915 1109 22-23 0.6611836557853188 1109 24-25 0.16627961758178955 1109 26-27 0.47973830955281827 1109 28-29 0.327854307224829 1109 30-31 0.1516552165231495 1109 32-33 0.4962669213502622 1109 34-35 0.36545441286610014 1109 36-37 0.4106421158611404 1109 38-39 0.7952922324724945 1109 40-41 0.8908227490363778 1109 42-43 0.7417533182021643 1109 44-45 0.1966387528712623 1109 46-47 0.5802125517927834 1109 48-49 -0.07571889053935976 1109 50-51 1.1587110146639112 1109 52-53 0.8056457542099373 1109 54-55 0.47724899559704426 1109 56-57 0.8125245105386192 1109 58-59 -0.2364273396845462 1109 60-61 0.4936484114653581 1109 62-63 0.21106864822272442 1109 64-65 0.1979754629641377 1109 66-67 -0.11182027045891019 1109 68-69 0.5112807386012683 1109 70-71 -0.274727630222678 1109 72-73 0.4132319947227252 1109 74-75 1.6462864230226089 1109 76 -0.6293481121497315 1110 1 1.3963768392314826 1110 2 1.8857049257153804 1110 3 0.8403079731174188 1110 4 0.7429555413947 1110 5 0.9256563589892508 1110 6 0.6009719415454313 1110 7 1.3537617666970476 1110 8 2.1269615903624164 1110 9 -0.3161386039581444 1110 10-11 1.815969449540578 1110 12-13 1.3686303739319996 1110 14-15 0.9054426259312329 1110 16-17 1.0368530683798518 1110 18-19 1.9091125892977452 1110 20-21 1.6067617750855234 1110 22-23 2.3132508979479702 1110 24-25 1.8409502218525766 1110 26-27 0.4482299042824849 1110 28-29 1.4207439028631832 1110 30-31 1.7135361797216966 1110 32-33 2.2935863216228682 1110 34-35 1.491329015319529 1110 36-37 2.016866559295945 1110 38-39 1.4755057717728128 1110 40-41 1.0266019403130677 1110 42-43 1.0439795789927153 1110 44-45 2.296843205392843 1110 46-47 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1.3177243375443801 2116 38-39 1.1963836707455933 2116 40-41 1.3839830691916042 2116 42-43 1.49632523185727 2116 44-45 2.7941163177512003 2116 46-47 0.7845297965174964 2116 48-49 1.0520278957812756 2116 50-51 1.6473843108074995 2116 52-53 2.018548980016387 2116 54-55 1.1826819327787064 2116 56-57 1.0918114375335257 2116 58-59 0.4834610910697634 2116 60-61 -0.5167566334048814 2116 62-63 1.9002027738594016 2116 64-65 1.460017665244031 2116 66-67 0.9030960526068235 2116 68-69 0.9239414225692535 2116 70-71 -1.380427363426655 2116 72-73 1.2793488998888627 2116 74-75 0.0811154310264861 2116 76 0.830462705216263 2117 1 -0.6238178583658645 2117 2 0.2769642463450417 2117 3 0.906588006257433 2117 4 0.2786846217592398 2117 5 -0.4186891256834926 2117 6 -0.7424414801612791 2117 7 -1.2438977196326917 2117 8 -1.0979762721065143 2117 9 0.1472002527112828 2117 10-11 -0.17548663993746416 2117 12-13 0.44723435073398576 2117 14-15 -0.22214643786329802 2117 16-17 -0.7915955645944699 2117 18-19 0.2193342131585574 2117 20-21 0.7307261495677118 2117 22-23 0.29227948121226177 2117 24-25 -0.027577118661096023 2117 26-27 -0.017439109802023012 2117 28-29 0.2167774571299539 2117 30-31 -0.1354178385053153 2117 32-33 -0.12128526081292534 2117 34-35 0.11410242045623065 2117 36-37 -0.9561036146891446 2117 38-39 0.0753918529658506 2117 40-41 -1.2232427158592571 2117 42-43 -1.9650303630122536 2117 44-45 -0.5480718733146972 2117 46-47 -0.9688149624387137 2117 48-49 -1.191254943670291 2117 50-51 0.793696783164858 2117 52-53 0.7023725094281552 2117 54-55 -0.7132159000386302 2117 56-57 -1.0536993983797878 2117 58-59 -0.3327983074264793 2117 60-61 -0.009624833316426162 2117 62-63 -0.6499133252118092 2117 64-65 0.18500107966242751 2117 66-67 -0.3644293786183006 2117 68-69 -0.5111813107345959 2117 70-71 -0.7069145372435486 2117 72-73 -0.6032528250116229 2117 74-75 -1.9727600665409604 2117 76 -0.6631242032313267 2118 1 -0.8807251667437619 2118 2 -0.24799119037509954 2118 3 -0.3362818689653828 2118 4 -0.9153884620197239 2118 5 -1.072209624792226 2118 6 -0.02408140886003096 2118 7 -0.2583807855863469 2118 8 -3.8805074664024133 2118 9 -1.621516324828825 2118 10-11 -1.673147067744953 2118 12-13 0.011182657329349865 2118 14-15 -0.5358719280593753 2118 16-17 -1.0107354932932218 2118 18-19 -1.3498502788200533 2118 20-21 -2.187945864692537 2118 22-23 -1.578821231800216 2118 24-25 -0.8095512719587781 2118 26-27 -0.2693330491959607 2118 28-29 -0.7552590847595262 2118 30-31 -0.5397181950115524 2118 32-33 0.04203915986443718 2118 34-35 -0.5756257435366372 2118 36-37 -1.0920216182542077 2118 38-39 -1.481760553451263 2118 40-41 -2.5147979743262816 2118 42-43 -1.4639162810158197 2118 44-45 -1.0290210711756593 2118 46-47 -0.7951295791945086 2118 48-49 -1.488157795720202 2118 50-51 -0.3750866392950343 2118 52-53 -1.5950873836199762 2118 54-55 -1.1586258822133182 2118 56-57 -0.30013433599119566 2118 58-59 -0.21726800439617477 2118 60-61 -2.712387756667585 2118 62-63 -0.15203008100503368 2118 64-65 -1.0604311841521863 2118 66-67 -2.8951780417199053 2118 68-69 -0.16904672962942513 2118 70-71 -1.791027727974388 2118 72-73 -2.5122323259028896 2118 74-75 -1.6036647011220673 2118 76 -1.2045680534987078 2119 1 0.15920806386743536 2119 2 -0.20035379951736942 2119 3 0.7645142275934269 2119 4 0.08563875934648024 2119 5 -0.03471604492576574 2119 6 -0.16866281615330436 2119 7 -1.2744311194332916 2119 8 0.14928195321751403 2119 9 0.6334913793314207 2119 10-11 0.4316295116078983 2119 12-13 -0.23509366521815878 2119 14-15 -0.8225950919011851 2119 16-17 -0.5239609683831006 2119 18-19 -0.31325551764902215 2119 20-21 -0.6183516171321877 2119 22-23 0.3140267394375833 2119 24-25 -0.35378599204095806 2119 26-27 -0.5672272453952445 2119 28-29 -0.7060166605171041 2119 30-31 -0.8019806500706181 2119 32-33 -0.6020928380811199 2119 34-35 0.1929284423904285 2119 36-37 -0.19750441229632187 2119 38-39 -0.1148499217101211 2119 40-41 -0.45573274576952727 2119 42-43 -1.5102696451657955 2119 44-45 -2.0941212252588635 2119 46-47 -0.6961953213619481 2119 48-49 -1.0163421819554372 2119 50-51 0.7576175209514986 2119 52-53 -0.8755188165937824 2119 54-55 -0.8409576747146019 2119 56-57 -0.5738888300846732 2119 58-59 -0.2830101718332614 2119 60-61 0.4251957050684183 2119 62-63 -0.7220095365777119 2119 64-65 -0.8407342144552175 2119 66-67 -0.2925201064348535 2119 68-69 -0.055859775340774576 2119 70-71 -0.6091453448208171 2119 72-73 -2.159895397294772 2119 74-75 -0.6719749219746589 2119 76 -0.9692059579671195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 22.0 13 174.0 14 529.0 15 978.0 16 1450.0 17 1754.0 18 2139.0 19 2008.0 20 1711.0 21 1334.0 22 1021.0 23 825.0 24 676.0 25 608.0 26 662.0 27 716.0 28 842.0 29 988.0 30 1232.0 31 1496.0 32 1932.0 33 2436.0 34 3416.0 35 5072.0 36 8415.0 37 9882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.15374694411827 22.71651042368477 10.675855165643227 29.453887466553734 2 16.4991016476165 28.533200810428532 30.66822126228067 24.299476279674302 3 14.901181237814901 31.117397454031114 27.539279024427536 26.442142283726444 4 10.663633930960664 27.715126725027716 32.4974196261325 29.123819717879122 5 10.397950992010397 35.55755189418556 31.283688214381282 22.76080889942276 6 21.304331205321304 36.91463740968691 16.50674719981651 25.274284185175272 7 20.78061087962078 31.92018043503192 19.59172751251959 27.70748117282771 8 17.934554073167934 38.48962116288849 17.98042738636798 25.595397377575598 9 19.056538858519055 22.686264765472686 19.1005007836691 39.156695592339155 10-11 20.194770442295194 27.690278680377688 25.664207347375662 26.45074352995145 12-13 20.074352995145077 25.50651783325051 24.924500172024924 29.494628999579493 14-15 19.223785312894222 27.07959019840208 22.826751787147824 30.869872701555867 16-17 20.175656561795176 29.23754730685424 23.42023777667342 27.166558354677168 18-19 19.026912343744026 28.08880308880309 23.944913796398946 28.93937077105394 20-21 18.791811613593794 28.4003593409534 24.08157804197408 28.726251003478726 22-23 21.15906571352116 27.91677816430292 23.704078902098704 27.220077220077222 24-25 19.643334989869643 29.883596467754884 23.44317443327344 27.02989410910203 26-27 18.88164685194388 29.984900034404987 24.811728277074813 26.321724836576323 28-29 19.81918269046982 28.343017699453345 26.564471118926562 25.27332849115027 30-31 19.65098054206965 28.265606483428268 26.357085515501353 25.726327459000725 32-33 18.20883825834321 30.553537979280556 25.500783669100503 25.736840093275738 34-35 19.52960740089453 29.014870599029013 25.31920180435032 26.136320195726135 36-37 19.854543369394857 28.37359990825337 25.80947283917581 25.96238388317596 38-39 19.305019305019304 26.89227416950189 27.634848426927633 26.167858098551168 40-41 18.593027256393594 26.73076187927673 27.223899996177224 27.452310868152452 42-43 17.342023777667343 26.907565273901906 27.932069268702932 27.81834167972782 44-45 17.359226270117357 26.865514736801867 28.367865744103366 27.407393248977407 46-47 17.394586949042395 27.865170686952865 27.715126725027716 27.025115638977027 48-49 17.31239726289231 28.241714132803242 27.02798272105203 27.41790588325242 50-51 17.134638174242134 27.78011391872778 26.605565962001602 28.479681945028478 52-53 16.128292365916128 28.042929775603042 27.28984288390229 28.538934974578538 54-55 16.094843075041094 28.535112198478537 26.453610612026452 28.916434114453914 56-57 17.06869528651707 27.98845521617799 26.42302840322642 28.51982109407852 58-59 17.11074582361711 27.739019075652738 26.050307733476053 29.099927367254104 60-61 16.74184792996674 26.77185672235177 26.403914522726403 30.082380824955084 62-63 16.94923353339195 27.346228831377346 25.979586375625978 29.724951259604726 64-65 17.35062502389235 27.85848082877786 24.603386979624602 30.187507167705185 66-67 17.107878741542105 29.04640850185405 24.092090676249093 29.753622080354752 68-69 17.602928246492603 28.664130891853663 23.64195879047364 30.09098207118009 70-71 17.548453687067546 29.326426851179328 23.411636530448412 29.713482931304714 72-73 17.604839634542603 28.864826637103864 23.183225658473184 30.347108069880345 74-75 18.123781490118123 29.472648037004472 22.66810657899767 29.73546389387974 76 18.2977178026683 29.12190832982912 22.946213540272947 29.634160327229637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 1.5 10 2.0 11 2.0 12 3.0 13 3.5 14 3.0 15 4.5 16 9.0 17 11.0 18 14.0 19 17.5 20 27.0 21 36.0 22 47.5 23 63.0 24 77.0 25 87.0 26 118.0 27 193.5 28 283.5 29 329.0 30 381.0 31 480.0 32 624.0 33 721.0 34 863.5 35 1101.0 36 1362.5 37 1529.0 38 1700.5 39 2075.5 40 2544.0 41 2893.0 42 2977.0 43 3157.0 44 3465.0 45 3628.5 46 3664.0 47 3665.0 48 3632.5 49 3387.5 50 3176.0 51 2990.5 52 2606.5 53 2241.5 54 2075.0 55 1939.0 56 1610.5 57 1287.5 58 1157.0 59 1011.0 60 785.5 61 591.5 62 477.0 63 412.5 64 304.5 65 213.0 66 141.0 67 117.0 68 96.5 69 67.0 70 45.5 71 33.0 72 26.0 73 18.5 74 15.5 75 13.0 76 12.5 77 10.5 78 7.0 79 5.0 80 4.5 81 5.5 82 8.5 83 10.0 84 7.0 85 3.0 86 2.5 87 3.0 88 3.0 89 2.5 90 2.0 91 1.5 92 1.0 93 1.0 94 1.5 95 2.0 96 2.0 97 4.0 98 3.5 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7053021904507053 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52318.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.47081310447648 #Duplication Level Percentage of deduplicated Percentage of total 1 84.76804639072185 64.82281432776482 2 8.515796840631875 13.024198172713024 3 3.291841631673665 7.551894185557552 4 1.4347130573885223 4.388546962804388 5 0.7798440311937612 2.9817653580029817 6 0.4699060187962407 2.1560457204021564 7 0.2549490101979604 1.3647310677013647 8 0.16496700659868027 1.0092128904010091 9 0.10497900419916018 0.7225046829007226 >10 0.21495700859828032 1.9782866317519785 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007645552200007646 0.0 2 0.0 0.0 0.0 0.007645552200007646 0.0 3 0.0 0.0 0.0 0.007645552200007646 0.0 4 0.0 0.0 0.0 0.011468328300011469 0.0 5 0.0 0.0 0.0 0.01337971635001338 0.0 6 0.0 0.0 0.0 0.01337971635001338 0.0 7 0.0 0.0 0.0 0.01337971635001338 0.0 8 0.0 0.0 0.0 0.01337971635001338 0.0 9 0.0 0.0 0.0 0.017202492450017204 0.0 10 0.0 0.0 0.0 0.019113880500019113 0.0 11 0.0 0.0 0.0 0.021025268550021025 0.0 12 0.0 0.0 0.0 0.022936656600022937 0.0 13 0.0 0.0 0.0 0.022936656600022937 0.0 14 0.0 0.0 0.0 0.022936656600022937 0.0 15 0.0 0.0 0.0 0.02484804465002485 0.0 16 0.0 0.0 0.0 0.02675943270002676 0.0 17 0.0 0.0 0.0 0.02675943270002676 0.0 18 0.0 0.0 0.0 0.030582208800030583 0.0 19 0.0 0.0 0.0 0.030582208800030583 0.0 20 0.0 0.0 0.0 0.030582208800030583 0.0 21 0.0 0.0 0.0 0.038227761000038225 0.0 22 0.0 0.0 0.0 0.04396192515004396 0.0 23 0.0 0.0 0.0 0.051607477350051605 0.0 24 0.0 0.0 0.0 0.05543025345005543 0.0 25 0.0 0.0 0.0 0.05734164150005734 0.0 26 0.0 0.0 0.0 0.06498719370006499 0.0 27 0.0 0.0 0.0 0.08792385030008792 0.0 28 0.0 0.0 0.0 0.10894911885010895 0.0 29 0.0 0.0 0.0 0.12997438740012998 0.0 30 0.0 0.0 0.0 0.16055659620016055 0.0 31 0.0 0.0 0.0 0.215986849650216 0.0 32 0.0 0.0 0.0 0.2714171031002714 0.0 33 0.0 0.0 0.0 0.3344929087503345 0.0 34 0.0 0.0 0.0 0.4071256546504071 0.0 35 0.0 0.0 0.0 0.5332772659505333 0.0 36 0.0 0.0 0.0 0.6747199816506747 0.0 37 0.0 0.0 0.0 0.9002637715509003 0.0 38 0.0 0.0 0.0 1.2844527696012844 0.0 39 0.0 0.0 0.0 1.7374517374517375 0.0 40 0.0 0.0 0.0 2.1847165411521847 0.0 41 0.0 0.0 0.0 2.8154745976528153 0.0 42 0.0 0.0 0.0 3.343017699453343 0.0 43 0.0 0.0 0.0 3.9183455025039184 0.0 44 0.0 0.0 0.0 4.445888604304446 0.0 45 0.0 0.0 0.0 5.046064452005046 0.0 46 0.0 0.0 0.0 5.694025000955694 0.0 47 0.0 0.0 0.0 6.313314729156313 0.0 48 0.0 0.0 0.0 6.915401964906915 0.0 49 0.0 0.0 0.0 7.4907297679574905 0.0 50 0.0 0.0 0.0 7.972399556557972 0.0 51 0.0 0.0 0.0 8.564929852058565 0.0 52 0.0 0.0 0.0 9.201422072709201 0.0 53 0.0 0.0 0.0 9.715585458159715 0.0 54 0.0 0.0 0.0 10.262242440460263 0.0 55 0.0 0.0 0.0 10.78978554226079 0.0 56 0.0 0.0 0.0 11.321151420161321 0.0 57 0.0 0.0 0.0 11.854428686111854 0.0 58 0.0 0.0 0.0 12.328452922512328 0.0 59 0.0 0.0 0.0 12.842616307962842 0.0 60 0.0 0.0 0.0 13.37971635001338 0.0 61 0.0 0.0 0.0 13.851829198363852 0.0 62 0.0 0.0 0.0 14.3984861806644 0.0 63 0.0 0.0 0.0 14.826637103864826 0.0 64 0.0 0.0 0.0 15.31977522076532 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTGGT 15 0.0022134965 69.99618 46 TGGATAA 15 0.0022134965 69.99618 68 GCGTAGC 15 0.0022134965 69.99618 24 AGGGTTG 25 2.3705636E-4 55.996944 5 AGACTAT 20 0.006916672 52.49713 70 TTCAATG 20 0.006916672 52.49713 32 GGTTTAA 20 0.006916672 52.49713 38 TTGAGGA 20 0.006916672 52.49713 2 GTAGCTG 20 0.006916672 52.49713 26 TCAATGA 20 0.006916672 52.49713 33 GATGGAT 20 0.006916672 52.49713 66 CGTAGCT 20 0.006916672 52.49713 25 TGCTATG 20 0.006916672 52.49713 60 TAGCTGG 20 0.006916672 52.49713 27 TGATGGA 20 0.006916672 52.49713 65 TTTACAC 40 4.536097E-5 43.74761 2 ATTAGAC 35 0.0012451471 39.997814 67 TAGATCT 35 0.0012451471 39.997814 39 TCTTTAA 40 0.0023990946 34.99809 23 GCTGGGT 50 0.007150503 27.998472 29 >>END_MODULE