FastQCFastQC Report
Wed 25 May 2016
SRR1780366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52318
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT30525.833556328605834No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT28245.397759853205398RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC9831.878894453151879No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC5040.9633395772009633No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG3140.6001758477006002No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT2730.5218089376505218No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT1800.344049849000344No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1420.27141710310027145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1400.2675943270002676No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1300.24848044650024848No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT1160.22172101380022172No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT1110.21216407355021216No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG970.1854046408501854No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC940.1796704767001797No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC860.16437937230016436No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT820.15673382010015674No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT810.15482243205015483No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC650.12424022325012424No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT610.11659467105011659No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG610.11659467105011659No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC560.10703773080010703No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGT150.002213017370.037
TTGGACG150.002213017370.054
TGGACGA150.002213017370.055
TGGAAAG150.002213017370.042
CATCTAG150.002213017370.036
GAGACAA150.002213017370.014
GGACGAA150.002213017370.056
GGATTCA150.002213017370.031
GACGAAC150.002213017370.057
GGGATTC150.002213017370.030
AGTGTAC150.002213017370.035
CACTTGG150.002213017370.051
ACGAACC150.002213017370.058
TTTACAC1650.063.6363642
GGAGACA252.3699235E-456.00000413
CAGTCTT252.3699235E-456.00000444
CGAACCA200.006915178652.559
CGAAACC200.006915178652.523
GGGTCTC200.006915178652.564
ACTTGGA200.006915178652.552
Produced by FastQC (version 0.11.5)