##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780366_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52318 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85949386444436 34.0 34.0 34.0 31.0 34.0 2 33.33206544592683 34.0 34.0 34.0 33.0 34.0 3 33.483867884857986 34.0 34.0 34.0 34.0 34.0 4 33.5013188577545 34.0 34.0 34.0 34.0 34.0 5 33.51471768798501 34.0 34.0 34.0 34.0 34.0 6 37.282598723192784 38.0 37.0 38.0 36.0 38.0 7 36.88290836805688 38.0 37.0 38.0 35.0 38.0 8 36.9013532627394 38.0 37.0 38.0 35.0 38.0 9 36.65619863144616 38.0 37.0 38.0 34.0 38.0 10-11 36.366537329408615 38.0 37.0 38.0 33.5 38.0 12-13 36.205789594403456 38.0 37.0 38.0 34.0 38.0 14-15 36.37467984250162 38.0 37.0 38.0 34.0 38.0 16-17 36.47748384877097 38.0 37.0 38.0 34.0 38.0 18-19 36.53086891700753 38.0 37.0 38.0 34.0 38.0 20-21 36.28523643870179 38.0 37.0 38.0 33.5 38.0 22-23 36.32081692725257 38.0 37.0 38.0 33.5 38.0 24-25 35.43163920639168 38.0 35.5 38.0 29.0 38.0 26-27 35.63805955885164 38.0 36.0 38.0 31.0 38.0 28-29 35.5476222332658 38.0 36.0 38.0 31.0 38.0 30-31 35.12826369509538 37.5 35.5 38.0 29.0 38.0 32-33 35.69104323559769 38.0 36.0 38.0 31.0 38.0 34-35 35.4475419549677 38.0 36.0 38.0 31.0 38.0 36-37 35.53038151305478 38.0 36.0 38.0 31.0 38.0 38-39 34.750506517833244 37.5 35.0 38.0 26.5 38.0 40-41 34.78366910050079 37.0 34.5 38.0 27.0 38.0 42-43 35.51035016629076 37.5 36.0 38.0 31.0 38.0 44-45 35.743807102717994 38.0 36.0 38.0 31.5 38.0 46-47 36.0006689858175 38.0 37.0 38.0 32.5 38.0 48-49 35.71366451316946 38.0 36.5 38.0 31.5 38.0 50-51 35.82225046829007 38.0 36.5 38.0 31.0 38.0 52-53 35.635211590657136 38.0 36.0 38.0 31.0 38.0 54-55 35.88045223441263 38.0 37.0 38.0 32.5 38.0 56-57 35.879295844642385 38.0 37.0 38.0 32.0 38.0 58-59 35.88045223441263 38.0 37.0 38.0 32.0 38.0 60-61 35.65703008524791 38.0 36.5 38.0 31.5 38.0 62-63 36.05500974807906 38.0 37.0 38.0 33.5 38.0 64-65 36.04598799648305 38.0 37.0 38.0 33.0 38.0 66-67 35.04638938797355 38.0 35.5 38.0 28.0 38.0 68-69 33.500554302534496 37.0 33.5 38.0 22.0 38.0 70-71 32.4981459535915 37.0 31.0 38.0 16.0 38.0 72-73 31.435863373982187 37.0 26.0 38.0 11.0 38.0 74-75 31.7001223288352 37.0 27.0 38.0 11.0 38.0 76 31.37014029588287 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04560363216595675 1101 2 0.4349898638315679 1101 3 0.13297569234408968 1101 4 0.2922897304425902 1101 5 0.3032879532061301 1101 6 -1.6006609340365472 1101 7 0.015646717705855906 1101 8 0.18207134946464976 1101 9 0.2295114780354197 1101 10-11 -0.009627372653191912 1101 12-13 0.4283895275593892 1101 14-15 0.6345136711329218 1101 16-17 0.18246404683487327 1101 18-19 -0.7915999225803887 1101 20-21 0.3210886666851067 1101 22-23 0.600125590511972 1101 24-25 -2.252295812565272 1101 26-27 -0.6777687578863336 1101 28-29 0.2902951749109235 1101 30-31 0.997996687716828 1101 32-33 -0.2353618178360648 1101 34-35 0.34521850196640713 1101 36-37 0.36215654126206687 1101 38-39 0.46259552543071436 1101 40-41 1.3366467815949221 1101 42-43 0.6073879662871917 1101 44-45 0.11170760067824403 1101 46-47 0.7043169115771235 1101 48-49 -2.0097032225055784 1101 50-51 -1.1092862379329986 1101 52-53 -2.501239775975513 1101 54-55 -1.1267216765744337 1101 56-57 -1.4367334341411109 1101 58-59 -1.7159960097574114 1101 60-61 -1.298318684472008 1101 62-63 -1.2592128475404891 1101 64-65 -1.888025622647966 1101 66-67 -2.300939019989805 1101 68-69 -2.120195630599042 1101 70-71 -1.854582478655452 1101 72-73 -1.9559939981432315 1101 74-75 -1.7952472231088414 1101 76 -1.2340323559047413 1102 1 -0.7155851790228525 1102 2 0.24757727641897986 1102 3 0.14288245225085205 1102 4 0.39660208475494585 1102 5 0.0933811932993649 1102 6 0.3785931452175362 1102 7 0.045366997426135924 1102 8 0.5067799741732699 1102 9 0.5439636924876368 1102 10-11 0.7814565434307212 1102 12-13 0.6463382455081046 1102 14-15 -0.5495189628997039 1102 16-17 0.15023793960876475 1102 18-19 -0.16828989927036275 1102 20-21 0.6080350536314896 1102 22-23 0.27308596347234015 1102 24-25 -4.983123318392781 1102 26-27 -1.1091790142965863 1102 28-29 0.13446766908341345 1102 30-31 1.8778917926119334 1102 32-33 0.4303724478982005 1102 34-35 0.34265439940231346 1102 36-37 -2.7558504517449265 1102 38-39 0.1618962247314144 1102 40-41 1.315259835207975 1102 42-43 1.0473063812056083 1102 44-45 1.0605420995127446 1102 46-47 0.9461584034186217 1102 48-49 -3.4821973949997513 1102 50-51 -0.7085869372336973 1102 52-53 0.011988662252925053 1102 54-55 0.4197818199290637 1102 56-57 0.7135579411502633 1102 58-59 -0.10515684891824861 1102 60-61 -6.338120549273874 1102 62-63 -2.4273946657222965 1102 64-65 -0.12829368791603457 1102 66-67 1.1758092317584499 1102 68-69 -1.9461863065897234 1102 70-71 -1.9024845765575549 1102 72-73 -4.364269056418291 1102 74-75 -1.9045129573745747 1102 76 -0.04801836989075525 1103 1 0.6738830708480776 1103 2 0.5765395892739065 1103 3 0.07292891585436223 1103 4 0.39789274040801814 1103 5 0.08481123976297056 1103 6 0.36527357887783296 1103 7 0.8422177976326424 1103 8 0.7135946360214902 1103 9 0.11562089434617917 1103 10-11 0.2209247933016556 1103 12-13 -0.7651227766911717 1103 14-15 -0.8865865932559274 1103 16-17 0.057362358813726644 1103 18-19 0.7184163475029948 1103 20-21 -0.1425070217428015 1103 22-23 0.5707885964098764 1103 24-25 -2.92468243042169 1103 26-27 -0.03207524558208519 1103 28-29 0.5969354026920826 1103 30-31 1.5497813124880295 1103 32-33 1.181508224872907 1103 34-35 0.7467844974921363 1103 36-37 -0.3935634099276797 1103 38-39 0.26140577558324196 1103 40-41 0.13283856520281745 1103 42-43 0.0764493858519657 1103 44-45 1.4257202099928676 1103 46-47 1.0383112170479407 1103 48-49 -1.713689392934704 1103 50-51 -0.22688843439426165 1103 52-53 0.6070325445451275 1103 54-55 0.3992087688191006 1103 56-57 0.7490251584966785 1103 58-59 -0.11181663208810022 1103 60-61 -4.33871425087429 1103 62-63 -1.5404819140444488 1103 64-65 -4.671520358030534E-4 1103 66-67 -0.2644075983912657 1103 68-69 0.049064080606981975 1103 70-71 0.6399263682023886 1103 72-73 -3.90133668677451 1103 74-75 -1.850692616641485 1103 76 -2.318230037417859 1104 1 -0.24558001640023974 1104 2 0.019492609408139572 1104 3 0.18031146618993432 1104 4 -0.816872360263126 1104 5 -0.28431627701555584 1104 6 0.20419975337448193 1104 7 0.2247681332031135 1104 8 0.5055409447463219 1104 9 0.954547068842821 1104 10-11 -1.138135609382907 1104 12-13 0.10736044478533557 1104 14-15 0.5127422658044765 1104 16-17 0.017093902437885333 1104 18-19 0.6311680253553433 1104 20-21 -0.6727083640246789 1104 22-23 -0.23793623580488799 1104 24-25 0.40082092528300706 1104 26-27 0.6323542846192538 1104 28-29 0.8855260067189334 1104 30-31 0.1101839970517915 1104 32-33 0.6043270168192123 1104 34-35 -0.508249059554835 1104 36-37 1.015832563226681 1104 38-39 0.8385869836369437 1104 40-41 1.6127043370148897 1104 42-43 0.31806012410699935 1104 44-45 0.576726921402269 1104 46-47 0.8671702774506258 1104 48-49 0.017854231226372974 1104 50-51 0.2697558609077575 1104 52-53 0.45267012843775234 1104 54-55 -0.402804653999695 1104 56-57 -0.14359229116775651 1104 58-59 -1.1889978401417878 1104 60-61 -0.4997880763776408 1104 62-63 -0.0639718469303503 1104 64-65 0.26127781440715125 1104 66-67 -0.858367329934886 1104 68-69 0.5624868993989196 1104 70-71 0.6567048916923213 1104 72-73 -0.4684507807342406 1104 74-75 -0.17284026764819416 1104 76 -1.217558896478259 1105 1 0.7600558466181724 1105 2 0.2962952251369302 1105 3 0.30954911891751635 1105 4 -0.26365432550147005 1105 5 0.06902221894038973 1105 6 0.2734649400893332 1105 7 0.6607516128107562 1105 8 -0.9239892565959593 1105 9 0.5913995899235331 1105 10-11 0.5955591075332833 1105 12-13 -1.4209694467995888 1105 14-15 -0.15657024495098426 1105 16-17 -0.1555312911604645 1105 18-19 0.4336331776527089 1105 20-21 0.0413683869648267 1105 22-23 -0.0557601903738103 1105 24-25 0.014312579043114226 1105 26-27 0.22928252416494388 1105 28-29 0.858185617801368 1105 30-31 1.1221225618427013 1105 32-33 0.331654499180253 1105 34-35 -2.0393968826489726 1105 36-37 -0.1917478876423644 1105 38-39 -0.4118217239865345 1105 40-41 0.3165418864900289 1105 42-43 -0.06743720853798152 1105 44-45 0.47913184310249335 1105 46-47 1.013466095726315 1105 48-49 -0.4578384206407762 1105 50-51 0.4959002422534766 1105 52-53 0.11327071353498042 1105 54-55 0.20952540967265065 1105 56-57 0.3007374283297608 1105 58-59 0.35509956133815734 1105 60-61 0.9362384250851008 1105 62-63 -1.0036767170043532 1105 64-65 0.3576037479814005 1105 66-67 0.42196307791229515 1105 68-69 -0.004519639923053376 1105 70-71 0.7270026029296233 1105 72-73 0.29662837947914866 1105 74-75 -0.3596411625027791 1105 76 -0.8569927288651122 1106 1 -0.6388883464436717 1106 2 0.33004333976739275 1106 3 0.3860951219341118 1106 4 0.25463375896308804 1106 5 0.06487440596905003 1106 6 0.5125297968012461 1106 7 -0.25440675822996894 1106 8 0.40689309634521464 1106 9 -0.481941284887931 1106 10-11 0.6802121995393193 1106 12-13 1.2617228608927178 1106 14-15 -0.4805709990988021 1106 16-17 0.5455433694730161 1106 18-19 0.4763367975622117 1106 20-21 0.5188382210523059 1106 22-23 0.5378484069112588 1106 24-25 -0.3330667573068098 1106 26-27 0.7940449676038597 1106 28-29 0.8823183478164509 1106 30-31 0.9959913401232399 1106 32-33 0.45146973296607484 1106 34-35 0.6920322274566075 1106 36-37 0.8818757925989686 1106 38-39 0.7249618355911451 1106 40-41 1.1679747673346697 1106 42-43 1.0457792318843389 1106 44-45 1.305112235259351 1106 46-47 1.2235905301154517 1106 48-49 1.3237415190274007 1106 50-51 0.34158274602422267 1106 52-53 0.7455293862348285 1106 54-55 0.18510987422770597 1106 56-57 0.3641801130959692 1106 58-59 0.9471055945206643 1106 60-61 1.468949586472732 1106 62-63 0.9667229813365239 1106 64-65 -0.9213366743413687 1106 66-67 0.4935128516679512 1106 68-69 1.9184630146772435 1106 70-71 0.2259656496867919 1106 72-73 0.24732596620614444 1106 74-75 -0.29959214289494085 1106 76 0.3000585531861688 1107 1 0.707086885754471 1107 2 0.1036501509129053 1107 3 0.24561524577311644 1107 4 0.1698814369816617 1107 5 0.098543136619206 1107 6 0.33992917760620145 1107 7 0.5640916937824159 1107 8 0.11548442761456812 1107 9 -0.39409298767430556 1107 10-11 0.7409707134712065 1107 12-13 0.255143913524293 1107 14-15 0.15913488325413994 1107 16-17 0.668608384952897 1107 18-19 0.8346959306891506 1107 20-21 0.44259983095943056 1107 22-23 -0.49756180980701004 1107 24-25 -0.07087635483002686 1107 26-27 -0.25781261753546403 1107 28-29 0.5539514747514289 1107 30-31 0.5975780274297477 1107 32-33 1.1925688041735043 1107 34-35 0.17964832652781837 1107 36-37 1.1378742446113534 1107 38-39 0.23831322878000094 1107 40-41 0.31232461253591026 1107 42-43 0.897559417642853 1107 44-45 0.509833597488452 1107 46-47 0.8694883629327848 1107 48-49 0.7616285159314273 1107 50-51 0.9274960587177148 1107 52-53 0.7474643707549475 1107 54-55 0.386735261224608 1107 56-57 0.9653798034984433 1107 58-59 0.43801116727608047 1107 60-61 0.7591972644913128 1107 62-63 0.6002369132250678 1107 64-65 0.49255651451311877 1107 66-67 -0.8783911730998497 1107 68-69 0.36525094037384065 1107 70-71 -0.9416040097559346 1107 72-73 -0.5760099471065452 1107 74-75 -1.3738955484676936 1107 76 -0.12099407839277987 1108 1 -0.8174014183390952 1108 2 0.12375249009419065 1108 3 -0.059574812706415514 1108 4 0.36615336680622335 1108 5 -0.1291615417433647 1108 6 -0.6063427522183602 1108 7 -0.2217270196678811 1108 8 -0.7017670343737379 1108 9 -0.13349357246963223 1108 10-11 0.39283474855892564 1108 12-13 1.0533895275593892 1108 14-15 0.3119126610319114 1108 16-17 -0.6568667612459365 1108 18-19 0.32866522893476713 1108 20-21 0.9473512929477295 1108 22-23 0.2014513480877298 1108 24-25 0.7410753995559318 1108 26-27 -0.43124098010855505 1108 28-29 -0.7163336129678655 1108 30-31 -0.10143513046499208 1108 32-33 0.5023275761033332 1108 34-35 0.6854962797441928 1108 36-37 0.9978888644943851 1108 38-39 1.1191611819963754 1108 40-41 0.7545760745242163 1108 42-43 -0.5775867811875557 1108 44-45 0.7221873986580434 1108 46-47 0.7971199418801547 1108 48-49 1.0670013229489612 1108 50-51 0.6542869943902332 1108 52-53 0.11113396139822385 1108 54-55 -0.8959767270794856 1108 56-57 0.006132727475055333 1108 58-59 0.6870974245860211 1108 60-61 1.0492191943158744 1108 62-63 0.9434386676110265 1108 64-65 0.5418986197762763 1108 66-67 0.48125794970716385 1108 68-69 0.9858649754615669 1108 70-71 -2.0187238927968707 1108 72-73 -1.827837432486664 1108 74-75 -2.0193633847250005 1108 76 -1.1929970023693883 1109 1 -1.0480913824968 1109 2 0.37164675532716984 1109 3 0.3148427500176041 1109 4 0.28618024852665513 1109 5 0.22142089553261712 1109 6 0.24459810799669413 1109 7 0.1418930520167052 1109 8 0.47601074340403926 1109 9 0.7352788289640628 1109 10-11 0.32798259802129337 1109 12-13 0.6714002802475534 1109 14-15 0.7192155284154325 1109 16-17 0.8303868229834563 1109 18-19 0.772280820332611 1109 20-21 0.9986057732344662 1109 22-23 0.6077013480877298 1109 24-25 0.3466533565451826 1109 26-27 0.43809145716743103 1109 28-29 -1.7164008172689407 1109 30-31 0.5015890630833937 1109 32-33 0.49029800621085684 1109 34-35 -0.2816632184636987 1109 36-37 0.8455143125230649 1109 38-39 1.0612758773368753 1109 40-41 0.8590563612625672 1109 42-43 0.9449490611063496 1109 44-45 0.8681103377261437 1109 46-47 0.9104263934930543 1109 48-49 0.31209540897047106 1109 50-51 -0.08216013822625001 1109 52-53 -0.31108826082400043 1109 54-55 -0.6188037880113839 1109 56-57 0.10239168804853449 1109 58-59 0.1536520840842357 1109 60-61 1.043641237326625 1109 62-63 0.4064763020196338 1109 64-65 0.5052050713891774 1109 66-67 0.03784397121254557 1109 68-69 -0.9178984653986504 1109 70-71 -0.5006235343739291 1109 72-73 -0.5987603715547642 1109 74-75 -1.0768454635780493 1109 76 -1.3120382210073807 1110 1 -1.1109264574627247 1110 2 -0.6860088557587005 1110 3 0.13692362234836253 1110 4 0.03311346070294263 1110 5 0.25004424855396934 1110 6 -0.05499298696014421 1110 7 0.7105890992679562 1110 8 0.4140389124181283 1110 9 0.8042020456837378 1110 10-11 -0.87374388993873 1110 12-13 0.2793284946955339 1110 14-15 0.8325503762118842 1110 16-17 0.3722314390670576 1110 18-19 0.03479785804274371 1110 20-21 0.7611228109445989 1110 22-23 -0.4486366800812789 1110 24-25 -0.7625924408196525 1110 26-27 1.0786866411746985 1110 28-29 0.056406997360767264 1110 30-31 -0.04025555300020045 1110 32-33 1.0130376700000383 1110 34-35 1.0611809432809665 1110 36-37 1.7086674247447817 1110 38-39 1.515092324406389 1110 40-41 -0.7415115624084905 1110 42-43 0.23100085574515106 1110 44-45 0.9022539088301897 1110 46-47 0.928281913711146 1110 48-49 1.6319954544043682 1110 50-51 -0.19347317775374506 1110 52-53 0.7727145560774105 1110 54-55 -0.4029700760622674 1110 56-57 0.7767215381166821 1110 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2116 48-49 0.02196686179608065 2116 50-51 -1.2506054450416784 2116 52-53 -0.3065769826285063 2116 54-55 -0.19268203200847012 2116 56-57 0.08192115688190427 2116 58-59 -1.8190377049043676 2116 60-61 -1.5269869460350094 2116 62-63 0.806063980894244 2116 64-65 -1.479247996765082 2116 66-67 -0.06213177460360697 2116 68-69 -0.8894483077464557 2116 70-71 1.956422306033538 2116 72-73 1.4164712559009693 2116 74-75 1.0852209928856915 2116 76 0.43445704942676855 2117 1 0.8611116535563283 2117 2 0.4918080456497478 2117 3 0.12874218075764077 2117 4 -0.5615427116251439 2117 5 -0.13365500579565293 2117 6 0.005176855624775101 2117 7 0.333828535887676 2117 8 0.18630486105109867 2117 9 -0.746647167240873 2117 10-11 -0.5550819181077387 2117 12-13 -0.9184241979308112 2117 14-15 0.034134883254139936 2117 16-17 -1.3882801599387449 2117 18-19 -1.9280749671436723 2117 20-21 -0.1980735436535781 2117 22-23 0.06726017161714282 2117 24-25 -2.9985079337773968 2117 26-27 -2.8566903265137853 2117 28-29 -1.3750345933600272 2117 30-31 -0.25400865987676013 2117 32-33 -1.6588243846809902 2117 34-35 0.8280616392213105 2117 36-37 -1.1291536191657414 2117 38-39 6.971297087901007E-4 2117 40-41 -0.7915840561947363 2117 42-43 -0.16745606223331322 2117 44-45 -0.35297600003476504 2117 46-47 -0.6661153522374974 2117 48-49 -0.5034643633255413 2117 50-51 -0.33488784221107437 2117 52-53 0.6021470332936545 2117 54-55 -0.3185665963605331 2117 56-57 0.6288859954489112 2117 58-59 -1.0124532290087487 2117 60-61 -0.7369327664684491 2117 62-63 -0.5590123127811282 2117 64-65 -0.9213366743413758 2117 66-67 -0.8153106777438168 2117 68-69 1.3890512499713665 2117 70-71 -0.3512402326661501 2117 72-73 0.6848259662061444 2117 74-75 0.17834903357564968 2117 76 -0.06023556446088918 2118 1 -0.30288121631889453 2118 2 0.11680804564974778 2118 3 -0.3670243076559103 2118 4 0.33016851832137917 2118 5 0.16692431684248987 2118 6 0.09630876293315538 2118 7 0.7504952025543403 2118 8 0.34873801613131405 2118 9 -2.1265491280251823 2118 10-11 -0.9944758575016763 2118 12-13 -1.9920650178951576 2118 14-15 0.49057427719353086 2118 16-17 -3.2569298925590644 2118 18-19 -0.39008477106523287 2118 20-21 -0.5008810302845959 2118 22-23 0.763004378390761 2118 24-25 1.0545223692529078 2118 26-27 -2.7914051215227005 2118 28-29 -0.607432097816357 2118 30-31 -0.6989730092528674 2118 32-33 -0.1861193935936427 2118 34-35 -0.11311483136692146 2118 36-37 -3.0052677011621682 2118 38-39 -0.1927075048723168 2118 40-41 0.033616478564624686 2118 42-43 0.5051152390144651 2118 44-45 -3.2670802781096313 2118 46-47 -0.20856187630165834 2118 48-49 0.25544829264593005 2118 50-51 -1.0600438136905765 2118 52-53 -2.2247246244603573 2118 54-55 -0.4638428886956447 2118 56-57 0.6693271719194982 2118 58-59 -0.3902384339998335 2118 60-61 -1.1202883814417035 2118 62-63 -1.0433037566313956 2118 64-65 0.2786410440186984 2118 66-67 0.32027310122231967 2118 68-69 -1.5103471457505577 2118 70-71 -0.6538249028978811 2118 72-73 0.8546120624628308 2118 74-75 0.3070255041638852 2118 76 0.6220282501558643 2119 1 0.777113647574275 2119 2 -0.7645478865536433 2119 3 0.20937528145554296 2119 4 -0.41174211341976275 2119 5 -0.34090824607481807 2119 6 -0.4640404923313568 2119 7 -0.5263409556377496 2119 8 0.0166887095057362 2119 9 -0.26920449525682244 2119 10-11 0.15890113273972162 2119 12-13 0.3676550642825447 2119 14-15 0.16019420528803607 2119 16-17 -0.29487288177324444 2119 18-19 -0.31946330213868634 2119 20-21 -1.2372061458469972 2119 22-23 0.1077013480877298 2119 24-25 1.0396276594429423 2119 26-27 0.3893216375938948 2119 28-29 0.19874407064794752 2119 30-31 1.0336284288570425 2119 32-33 0.4664871806231048 2119 34-35 0.2449360160907048 2119 36-37 0.4012551545132226 2119 38-39 -2.1402550138605037 2119 40-41 -1.5316762795247456 2119 42-43 -0.17374968137588098 2119 44-45 -0.04558567102180433 2119 46-47 0.42953519611744184 2119 48-49 -0.1408023493674193 2119 50-51 0.9342422674599149 2119 52-53 0.45247106120989855 2119 54-55 0.1641103727321891 2119 56-57 -0.9768592687584672 2119 58-59 -0.0985075659977781 2119 60-61 0.7379550700220889 2119 62-63 -0.6240048352138317 2119 64-65 -0.12229629547796605 2119 66-67 1.0324585146789218 2119 68-69 0.9420796647271032 2119 70-71 0.7251555798961604 2119 72-73 0.5649356371931837 2119 74-75 -0.07433042803950585 2119 76 -0.08765331122060971 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 1.0 21 3.0 22 5.0 23 17.0 24 58.0 25 98.0 26 182.0 27 340.0 28 559.0 29 931.0 30 1384.0 31 2074.0 32 2983.0 33 4383.0 34 6069.0 35 7499.0 36 9594.0 37 16136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.13043312053213 22.844909973622844 11.227493405711227 33.7971635001338 2 15.581635383615582 17.13177109216713 47.00867770174701 20.277915822470277 3 14.365992583814366 27.124507817577125 38.080584120188085 20.42891547842043 4 10.365457395160366 24.676019725524675 35.13895791123514 29.81956496807982 5 9.93921786000994 40.1009212890401 37.58935739133759 12.37050345961237 6 20.537864597270538 39.579112351389576 16.701708780916704 23.18131427042318 7 20.26071333002026 37.45556022783745 19.06036163461906 23.22336480752322 8 17.791199969417793 36.973890439236975 23.69165487977369 21.543254711571542 9 17.758706372567758 19.662448870369662 17.982338774417983 44.596505982644594 10-11 18.453495928743454 25.092702320425094 31.673611376581672 24.78019037424978 12-13 22.29060743912229 18.677128330593675 28.14232195420314 30.88994227608089 14-15 22.98061852517298 22.908941473297908 20.21961848694522 33.89082151458389 16-17 24.94265835849994 27.439886845827438 19.626132497419626 27.991322298252992 18-19 22.924232577697925 26.225199740051224 19.903283764669904 30.947283917580947 20-21 20.88955999847089 23.664895447073665 20.023701211820025 35.42184334263542 22-23 23.28070644902328 29.923735616804926 20.533086127145534 26.262471807026262 24-25 24.691310829924692 32.004281509232 20.564624029970567 22.73978363087274 26-27 26.0971367407011 29.074123628579073 24.464811345999465 20.363928284720366 28-29 28.025727283153024 22.235177185672235 27.51060820367751 22.22848732749723 30-31 31.391681639206393 20.13934018884514 30.251538667380252 18.217439504568215 32-33 24.020413624374022 21.02049007989602 33.96249856645896 20.996597729270995 34-35 17.553232157192554 24.614855307924614 38.16755227646317 19.664360258419663 36-37 16.838373026491837 27.15986849650216 29.204098015979202 26.797660461026794 38-39 20.265491800145266 23.024580450323022 28.38220115447838 28.327726595053328 40-41 19.066095798769066 24.025192094499026 31.242593371306242 25.666118735425663 42-43 25.632669444550633 22.40720211017241 32.746855766657745 19.213272678619216 44-45 18.991551664818992 25.18253755877518 29.939982415229938 25.885928361175885 46-47 25.467334378225466 20.12882755457013 27.331893421002334 27.071944646202073 48-49 22.657593944722656 19.585993348369584 29.113307083604113 28.643105623303644 50-51 17.054359876142055 19.66913872854467 34.27405481860927 29.002446576704006 52-53 18.38086318284338 20.25211208379525 38.860430444588864 22.506594288772508 54-55 16.90909438434191 20.12882755457013 35.09021751596009 27.871860545127873 56-57 23.676363775373677 20.136473106770136 30.335639741580334 25.851523376275853 58-59 17.758706372567758 28.052486715853053 33.56492985205857 20.623877059520623 60-61 19.893726824419893 37.142092587637144 23.62953476814863 19.334645819794336 62-63 14.930807752589931 44.08329829121908 21.306242593371305 19.67965136281968 64-65 14.506479605489506 45.01127718949501 20.545510149470545 19.936733055544938 66-67 14.659390649489659 44.478955617569476 20.020834129745023 20.84081960319584 68-69 14.964257043464965 42.15853052486716 20.468098933445468 22.40911349822241 70-71 15.870254979165871 39.12993615963913 21.028135632096028 23.971673229098972 72-73 16.319431170916317 35.60915937153561 21.721013800221723 26.350395657326352 74-75 16.80317293448655 34.5104410570077 22.305156018540643 26.381229989965117 76 16.62143048281662 34.47761764593448 22.48939179632249 26.41156007492641 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 0.5 15 3.0 16 4.0 17 2.0 18 5.5 19 9.5 20 13.0 21 16.0 22 27.5 23 49.5 24 58.5 25 57.0 26 92.5 27 150.5 28 199.5 29 226.0 30 260.5 31 359.0 32 511.0 33 599.0 34 721.0 35 917.0 36 1079.0 37 1167.0 38 1356.0 39 1897.0 40 3279.5 41 5090.0 42 5870.0 43 5750.0 44 5341.0 45 4520.0 46 3988.0 47 3678.5 48 3165.5 49 2727.5 50 2493.0 51 2345.0 52 1931.0 53 1590.5 54 1516.0 55 1419.5 56 1121.5 57 821.0 58 722.0 59 608.5 60 417.0 61 283.0 62 227.0 63 191.0 64 131.0 65 93.5 66 55.5 67 31.0 68 23.5 69 12.0 70 5.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 9.556940250009556E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52318.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.88218204059788 #Duplication Level Percentage of deduplicated Percentage of total 1 68.99924154724363 33.03834244428304 2 16.063231008742164 15.382851026415384 3 6.798131811105346 9.765281547459766 4 3.0737295916330685 5.887075194005887 5 1.7963354756297154 4.300623112504301 6 1.0298990060277033 2.958828701402959 7 0.6267214881641452 2.100615466952101 8 0.4351123707636422 1.6667303796016668 9 0.26346253642569156 1.1353645017011353 >10 0.8303061754021795 5.6978477770556974 >50 0.03592670951259431 1.3054780381513054 >100 0.031934852900083834 2.6874115983026874 >500 0.007983713225020958 2.8422340303528424 >1k 0.007983713225020958 11.23131618181123 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 3052 5.833556328605834 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 2824 5.397759853205398 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 983 1.878894453151879 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 504 0.9633395772009633 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 314 0.6001758477006002 No Hit TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 273 0.5218089376505218 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT 180 0.344049849000344 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 142 0.27141710310027145 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 140 0.2675943270002676 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 130 0.24848044650024848 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT 116 0.22172101380022172 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT 111 0.21216407355021216 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 97 0.1854046408501854 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 94 0.1796704767001797 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 86 0.16437937230016436 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT 82 0.15673382010015674 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT 81 0.15482243205015483 No Hit CTTATCACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 65 0.12424022325012424 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGTCTT 61 0.11659467105011659 No Hit CTTATTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 61 0.11659467105011659 No Hit TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 56 0.10703773080010703 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.011468328300011469 0.0 2 0.0 0.0 0.0 0.01337971635001338 0.0 3 0.0 0.0 0.0 0.01337971635001338 0.0 4 0.0 0.0 0.0 0.015291104400015292 0.0 5 0.0 0.0 0.0 0.017202492450017204 0.0 6 0.0 0.0 0.0 0.017202492450017204 0.0 7 0.0 0.0 0.0 0.017202492450017204 0.0 8 0.0 0.0 0.0 0.017202492450017204 0.0 9 0.0 0.0 0.0 0.022936656600022937 0.0 10 0.0 0.0 0.0 0.02484804465002485 0.0 11 0.0 0.0 0.0 0.02484804465002485 0.0 12 0.0 0.0 0.0 0.02675943270002676 0.0 13 0.0 0.0 0.0 0.02675943270002676 0.0 14 0.0 0.0 0.0 0.02675943270002676 0.0 15 0.0 0.0 0.0 0.030582208800030583 0.0 16 0.0 0.0 0.0 0.032493596850032495 0.0 17 0.0 0.0 0.0 0.032493596850032495 0.0 18 0.0 0.0 0.0 0.03440498490003441 0.0 19 0.0 0.0 0.0 0.038227761000038225 0.0 20 0.0 0.0 0.0 0.038227761000038225 0.0 21 0.0 0.0 0.0 0.045873313200045875 0.0 22 0.0 0.0 0.0 0.0496960893000497 0.0 23 0.0 0.0 0.0 0.06307580565006307 0.0 24 0.0 0.0 0.0 0.06880996980006882 0.0 25 0.0 0.0 0.0 0.07454413395007455 0.0 26 0.0 0.0 0.0 0.0821896861500822 0.0 27 0.0 0.0 0.0 0.10321495470010321 0.0 28 0.0 0.0 0.0 0.12997438740012998 0.0 29 0.0 0.0 0.0 0.15864520815015865 0.0 30 0.0 0.0 0.0 0.1854046408501854 0.0 31 0.0 0.0 0.0 0.23127795405023127 0.0 32 0.0 0.0 0.0 0.2905309836002905 0.0 33 0.0 0.0 0.0 0.3516954012003517 0.0 34 0.0 0.0 0.0 0.4205053710004205 0.0 35 0.0 0.0 0.0 0.5447455942505447 0.0 36 0.0 0.0 0.0 0.6900110860506901 0.0 37 0.0 0.0 0.0 0.927023204250927 0.0 38 0.0 0.0 0.0 1.3112122023013113 0.0 39 0.0 0.0 0.0 1.7642111701517642 0.0 40 0.0 0.0 0.0 2.2363240185022364 0.0 41 0.0 0.0 0.0 2.8957528957528957 0.0 42 0.0 0.0 0.0 3.4385871019534386 0.0 43 0.0 0.0 0.0 4.055965442104056 0.0 44 0.0 0.0 0.0 4.616002140754616 0.0 45 0.0 0.0 0.0 5.262051301655262 0.0 46 0.0 0.0 0.0 5.913834626705913 0.0 47 0.0 0.0 0.0 6.540769907106541 0.0 48 0.0 0.0 0.0 7.1696165755571695 0.0 49 0.0 0.0 0.0 7.815665736457816 0.0 50 0.0 0.0 0.0 8.364234106808365 0.0 51 0.0 0.0 0.0 8.97970105890898 0.0 52 0.0 0.0 0.0 9.66971214495967 0.0 53 0.0 0.0 0.0 10.216369127260217 0.0 54 0.0 0.0 0.0 10.801253870560801 0.0 55 0.0 0.0 0.0 11.367024733361367 0.0 56 0.0 0.0 0.0 11.936618372261936 0.0 57 0.0 0.0 0.0 12.513857563362514 0.0 58 0.0 0.0 0.0 13.012729844413013 0.0 59 0.0 0.0 0.0 13.557475438663557 0.0 60 0.0 0.0 0.0 14.132803241714132 0.0 61 0.0 0.0 0.0 14.622118582514622 0.0 62 0.0 0.0 0.0 15.176421117015176 0.0 63 0.0 0.0 0.0 15.623685920715623 0.0 64 0.0 0.0 0.0 16.13784930616614 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTAGT 15 0.0022130173 70.0 37 TTGGACG 15 0.0022130173 70.0 54 TGGACGA 15 0.0022130173 70.0 55 TGGAAAG 15 0.0022130173 70.0 42 CATCTAG 15 0.0022130173 70.0 36 GAGACAA 15 0.0022130173 70.0 14 GGACGAA 15 0.0022130173 70.0 56 GGATTCA 15 0.0022130173 70.0 31 GACGAAC 15 0.0022130173 70.0 57 GGGATTC 15 0.0022130173 70.0 30 AGTGTAC 15 0.0022130173 70.0 35 CACTTGG 15 0.0022130173 70.0 51 ACGAACC 15 0.0022130173 70.0 58 TTTACAC 165 0.0 63.636364 2 GGAGACA 25 2.3699235E-4 56.000004 13 CAGTCTT 25 2.3699235E-4 56.000004 44 CGAACCA 20 0.0069151786 52.5 59 CGAAACC 20 0.0069151786 52.5 23 GGGTCTC 20 0.0069151786 52.5 64 ACTTGGA 20 0.0069151786 52.5 52 >>END_MODULE