FastQCFastQC Report
Wed 25 May 2016
SRR1780365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558394
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC207.893757E-443.95376641
AACGACC353.2440553E-431.381492
CTTAGAC502.3761986E-630.7703951
GGTCCGT300.00575178129.3235323
GTCCGTC407.02266E-427.5031424
CGGTGGT1650.025.30444743
GTTCGCA450.001391239524.46259735
ACGGTGC551.5687497E-424.05236415
GCGCTAT551.5725804E-424.04371619
CGTGCGA752.0476855E-623.48409310
TTAATAC752.087996E-623.4315132
CTTATAA952.7062924E-823.1356351
CGTAGCG703.1577598E-522.04815
CGGAATC703.1621457E-522.04403720
GGCGCTA602.8349727E-422.04007518
CTATACA1306.91216E-1121.9788531
TCGGTGG3050.020.89418242
TCGGAGT755.5102864E-520.51175931
TACGGAC654.9029733E-420.32277138
AAGGTAA1101.2527198E-720.0148537
Produced by FastQC (version 0.11.5)