FastQCFastQC Report
Wed 25 May 2016
SRR1780365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558394
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT357106.395126022127745No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT337966.052357296102752RNA PCR Primer, Index 45 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC109571.9622345512308514RNA PCR Primer, Index 45 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC58951.05570618595472No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG40790.7304877917742669No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT28330.5073478583222599RNA PCR Primer, Index 45 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC19260.34491774625085514No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC17260.30910074248648806No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG15240.27292556868447726No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC12420.22242359337671966No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG10870.19466541545933516No Hit
CTTAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC9240.16547455739137598RNA PCR Primer, Index 45 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC6650.11909153751652059No Hit
CTTATCACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC6160.11031637159425066No Hit
CTTATTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG5720.1024366307660899No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG5590.10010852552140602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAGA254.4354092E-544.00752314
ATTAGAG454.783942E-1044.0035863
CCGTCTT35700.042.51685344
CTTATAA950.039.3927961
GCCGTCT39050.038.86944243
TTTACAC12800.038.8469122
CTTTACA13350.037.7611921
TTACACA13850.036.06073
CTATACA8200.035.7050171
TAGACTC250.002351805835.19971517
CTTTATA3300.034.6694872
TCTTTAT3400.033.020431
CGTGCGA502.3553039E-630.80526710
CTTATAC53400.030.7118871
TTATACA54450.029.9418332
ATACTGT300.00574262829.3330966
GCGAAGT300.00574514229.33046732
ACATCTT300.00574514229.33046737
TGATGAC300.00574514229.33046731
TTTATAC4350.028.8299353
Produced by FastQC (version 0.11.5)