##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780364_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9344 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.27878852739726 23.0 12.0 31.0 12.0 32.0 2 19.954837328767123 21.0 11.0 27.0 11.0 33.0 3 20.56228595890411 21.0 11.0 31.0 11.0 33.0 4 21.12339469178082 23.0 11.0 32.0 11.0 34.0 5 20.754601883561644 21.0 11.0 31.0 11.0 34.0 6 22.846104452054796 23.0 11.0 34.0 11.0 36.0 7 22.896511130136986 23.0 11.0 34.0 11.0 36.0 8 22.96586044520548 23.0 11.0 34.0 11.0 36.0 9 22.514875856164384 22.0 12.0 33.0 12.0 36.0 10-11 20.992776113013697 21.0 11.0 31.0 11.0 35.5 12-13 20.762200342465754 21.0 11.0 29.5 11.0 34.0 14-15 20.38960830479452 21.0 11.0 29.5 11.0 34.0 16-17 19.864779537671232 16.5 11.0 27.5 11.0 34.0 18-19 20.239940068493148 21.0 11.0 28.0 11.0 34.0 20-21 20.363388270547944 16.0 11.0 29.0 11.0 34.0 22-23 19.68621575342466 16.0 11.0 27.5 10.5 34.0 24-25 19.664008989726028 16.0 11.0 27.0 10.5 34.0 26-27 19.502140410958905 21.0 11.0 26.5 10.5 34.0 28-29 18.974850171232877 11.0 11.0 26.0 11.0 34.0 30-31 18.869488441780824 11.0 11.0 26.0 11.0 34.0 32-33 19.288741438356162 16.0 11.0 26.5 11.0 34.0 34-35 19.63811001712329 16.0 11.0 27.0 11.0 34.0 36-37 19.278146404109588 11.0 11.0 26.0 11.0 34.0 38-39 18.631956335616437 11.0 11.0 26.0 11.0 34.0 40-41 18.99117080479452 11.0 11.0 26.0 11.0 34.0 42-43 19.233251284246577 15.0 11.0 26.0 11.0 34.0 44-45 18.971050941780824 11.0 11.0 26.0 11.0 34.0 46-47 19.167326626712327 11.0 11.0 26.5 11.0 34.0 48-49 19.469231592465754 15.5 11.0 26.5 11.0 34.0 50-51 19.763217037671232 21.0 11.0 27.0 11.0 34.0 52-53 19.519852311643838 19.5 11.0 26.0 11.0 34.0 54-55 19.504066780821915 20.0 11.0 26.0 11.0 34.0 56-57 18.87992294520548 15.0 11.0 26.0 11.0 33.5 58-59 18.95660316780822 16.0 11.0 26.0 11.0 33.5 60-61 19.306560359589042 19.0 11.0 26.0 11.0 33.0 62-63 18.82956977739726 15.0 11.0 26.0 11.0 33.0 64-65 19.09637200342466 15.0 11.0 25.5 11.0 33.0 66-67 19.316513270547944 19.0 11.0 26.0 11.0 33.0 68-69 18.928082191780824 15.0 11.0 25.5 11.0 33.0 70-71 19.087542808219176 19.0 11.0 25.0 11.0 33.0 72-73 19.019210188356162 15.0 11.0 25.0 11.0 33.0 74-75 18.875267551369863 15.0 11.0 25.5 11.0 32.5 76 18.250321061643834 11.0 11.0 24.0 11.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23378040813358325 1101 2 -0.6344627533857654 1101 3 1.9748516723514946 1101 4 0.6488269666456787 1101 5 -1.3673883322279146 1101 6 -0.8968685645237109 1101 7 2.7603056299615965 1101 8 1.6571349103593782 1101 9 -0.1394678990245417 1101 10-11 2.178240485045265 1101 12-13 2.4374002456269572 1101 14-15 1.5866190215563414 1101 16-17 2.1282500642381095 1101 18-19 -0.17017412239286145 1101 20-21 1.5772583609882425 1101 22-23 1.2255415004751171 1101 24-25 0.4461313794073938 1101 26-27 0.7798501123243042 1101 28-29 1.811366884969889 1101 30-31 2.868253229455874 1101 32-33 3.29427131306198 1101 34-35 1.178754525866431 1101 36-37 1.503307717079089 1101 38-39 1.9389613689828664 1101 40-41 1.0590770135835825 1101 42-43 0.4364215732024874 1101 44-45 2.687235651020373 1101 46-47 3.6143270170594697 1101 48-49 1.928208656777315 1101 50-51 -0.21160036227567147 1101 52-53 1.2412157802492239 1101 54-55 3.0208194401468234 1101 56-57 -0.26166139219603934 1101 58-59 0.8681979884196664 1101 60-61 0.7988359650612296 1101 62-63 0.422215507094176 1101 64-65 0.5699450346534825 1101 66-67 1.3716568294255076 1101 68-69 -1.9145020253568106 1101 70-71 0.021634493877090932 1101 72-73 0.32614788646586845 1101 74-75 2.7994518775322916 1101 76 0.3016152722250318 1102 1 2.5204148686810868 1102 2 -0.9082147662682161 1102 3 -1.4325557350559137 1102 4 -0.12895081113209983 1102 5 -1.2611081389912009 1102 6 -1.188333942945608 1102 7 -3.2042676389594966 1102 8 -3.2156509189481888 1102 9 2.33396205266628 1102 10-11 -1.6929350383041708 1102 12-13 -2.961150479010726 1102 14-15 0.3901616946642328 1102 16-17 -0.5255341547634984 1102 18-19 -0.1886926409113805 1102 20-21 -0.663482379752498 1102 22-23 0.7182951236635233 1102 24-25 -1.2301971230080646 1102 26-27 -3.0872996461298037 1102 28-29 -0.06222409731674361 1102 30-31 -1.9562234211077332 1102 32-33 -3.4086272376626567 1102 34-35 -1.4540957156794612 1102 36-37 -0.7446794004732453 1102 38-39 0.05812400988463651 1102 40-41 -2.517412519427687 1102 42-43 -0.9862837408071741 1102 44-45 1.6026945882184371 1102 46-47 0.5700436032108378 1102 48-49 -0.13781388750609835 1102 50-51 -1.738975563563919 1102 52-53 -0.5703784226493269 1102 54-55 1.2213025319342634 1102 56-57 -0.6110977851106938 1102 58-59 -0.1752802724498963 1102 60-61 -1.4282171750353854 1102 62-63 -1.3128891627931054 1102 64-65 -1.6313432101130267 1102 66-67 -2.9407425264521088 1102 68-69 -2.7937290784969058 1102 70-71 -0.11524151256413262 1102 72-73 -1.2463158816500766 1102 74-75 -0.9517558519363085 1102 76 -2.429463632766918 1103 1 -1.7143677400145663 1103 2 0.9824684635454517 1103 3 1.3960156935155155 1103 4 -2.071807953989243 1103 5 -1.76421372905331 1103 6 -0.4852283528834995 1103 7 -4.286255216599248 1103 8 -0.31006085683638673 1103 9 -1.5616901212467624 1103 10-11 -1.6196431128383324 1103 12-13 -1.6673616591349507 1103 14-15 0.10196293690025726 1103 16-17 -0.8267763907883463 1103 18-19 -0.660121212339952 1103 20-21 0.06508904881892974 1103 22-23 -2.0363632614296456 1103 24-25 -1.9792654459894337 1103 26-27 -1.723324490850299 1103 28-29 -0.6261992525962512 1103 30-31 -2.0767203155176723 1103 32-33 -1.3104905916999243 1103 34-35 -0.8894994423875353 1103 36-37 -0.9993377855664178 1103 38-39 -0.6747952447737511 1103 40-41 -1.7261081716016022 1103 42-43 0.4776914144723321 1103 44-45 -0.8302246664399462 1103 46-47 0.21538521811767097 1103 48-49 -2.299304570735913 1103 50-51 -1.447050097725409 1103 52-53 -3.077831838798396 1103 54-55 -0.9426726233452385 1103 56-57 -3.1881164186510667 1103 58-59 -3.174659154437478 1103 60-61 -2.102751336526069 1103 62-63 0.41692450180316953 1103 64-65 -1.6797904150819711 1103 66-67 -0.8177611599924823 1103 68-69 -0.6160893269441132 1103 70-71 -1.5270427548001564 1103 72-73 -1.5500425897246117 1103 74-75 -3.156103678023266 1103 76 -1.9375381669284089 1104 1 0.6522989266521009 1104 2 -1.42705534597836 1104 3 -0.29922240172258086 1104 4 -0.6289508111320998 1104 5 3.5215005566609747 1104 6 0.7623906947355508 1104 7 0.6566019262578955 1104 8 2.108986762211231 1104 9 0.17164321208657185 1104 10-11 -1.2958335890288097 1104 12-13 -1.3125997543730428 1104 14-15 0.9495819845193054 1104 16-17 0.4541759901640354 1104 18-19 0.36130735908861666 1104 20-21 -1.7301490464191644 1104 22-23 0.7255415004751171 1104 24-25 0.7516869349629474 1104 26-27 -0.609038776564585 1104 28-29 -0.5404849668819622 1104 30-31 3.142327303529946 1104 32-33 -0.8557286869380185 1104 34-35 -1.9990232519113462 1104 36-37 -2.8040996903283215 1104 38-39 -1.346223816202322 1104 40-41 -0.3427748382682694 1104 42-43 0.5753104620913803 1104 44-45 0.781680095464818 1104 46-47 0.010623313355765163 1104 48-49 -0.24216171359305605 1104 50-51 -2.2708596215349353 1104 52-53 -0.4921175530841069 1104 54-55 -0.6569583376309538 1104 56-57 -0.5190687996034455 1104 58-59 1.051531321753 1104 60-61 2.904391520616784 1104 62-63 -0.7592659743873043 1104 64-65 -0.18931422460578062 1104 66-67 -1.065380207611529 1104 68-69 -2.17561313646792 1104 70-71 0.14200486424746472 1104 72-73 -1.1738521135341315 1104 74-75 -0.6561036780232641 1104 76 -2.5613476907379322 1105 1 -2.1810344066812313 1105 2 -2.5937220126450278 1105 3 -0.8992224017225787 1105 4 1.0710491888678995 1105 5 1.4215005566609733 1105 6 -0.9709426385977835 1105 7 -2.3767314070754395 1105 8 -1.5910132377887685 1105 9 -3.0616901212467624 1105 10-11 -1.5791669223621412 1105 12-13 0.5540669122936244 1105 14-15 -0.6004180154806953 1105 16-17 -0.862490676502631 1105 18-19 -1.038692640911382 1105 20-21 0.16985095358083413 1105 22-23 -1.2744584995248793 1105 24-25 0.31835360162961734 1105 26-27 0.3576278901020835 1105 28-29 -0.5238183002152965 1105 30-31 0.8423273035299488 1105 32-33 -1.95572868693802 1105 34-35 0.7176434147553188 1105 36-37 -1.1207663569949844 1105 38-39 -1.2628904828689862 1105 40-41 0.3738918283983992 1105 42-43 -0.12468953790862258 1105 44-45 -3.084986571201849 1105 46-47 0.043956646689100154 1105 48-49 0.10783828640694537 1105 50-51 0.9791403784650647 1105 52-53 0.7078824469158924 1105 54-55 0.0430416623690455 1105 56-57 1.630931200396553 1105 58-59 0.4348646550863329 1105 60-61 0.837724853950121 1105 62-63 -0.3925993077206371 1105 64-65 -0.8226475579391099 1105 66-67 0.21795312572180592 1105 68-69 -2.6089464698012534 1105 70-71 -1.5246618024192067 1105 72-73 -0.9571854468674701 1105 74-75 -0.0894370113566012 1105 76 0.4719856425953992 1106 1 -2.354367740014567 1106 2 -0.4803886793116945 1106 3 0.08744426494408586 1106 4 1.9910491888679012 1106 5 0.32150055666097543 1106 6 1.629057361402218 1106 7 -1.7833980737421058 1106 8 -1.0643465711221012 1106 9 -1.9216901212467619 1106 10-11 5.137499744304524 1106 12-13 0.7640669122936217 1106 14-15 1.8362486511859686 1106 16-17 -1.0124906765026296 1106 18-19 0.011307359088618796 1106 20-21 0.5565176202475008 1106 22-23 1.4122081671417845 1106 24-25 -0.2249797317037192 1106 26-27 0.7509612234354144 1106 28-29 -0.20048496688196238 1106 30-31 0.46899397019661393 1106 32-33 -1.499062020271353 1106 34-35 0.7876434147553191 1106 36-37 -0.6107663569949864 1106 38-39 -0.14622381620231906 1106 40-41 -0.006108171601603374 1106 42-43 1.7919771287580453 1106 44-45 -0.22165323786851943 1106 46-47 0.8639566466890969 1106 48-49 0.8178382864069427 1106 50-51 1.2358070451317325 1106 52-53 1.607882446915891 1106 54-55 1.6030416623690442 1106 56-57 -0.6824021329367795 1106 58-59 0.7081979884196663 1106 60-61 2.0743915206167856 1106 62-63 -0.1659326410539741 1106 64-65 -2.502647557939113 1106 66-67 -2.992046874278195 1106 68-69 -1.8389464698012539 1106 70-71 1.558671530914129 1106 72-73 -1.3271854468674675 1106 74-75 1.4638963219767334 1106 76 0.6453189759287348 1107 1 -1.1956177400145656 1107 2 -2.310388679311693 1107 3 -0.4638057350559137 1107 4 -2.3477008111321 1107 5 -1.0409994433390253 1107 6 1.2790573614022165 1107 7 2.150351926257894 1107 8 0.11315342887789726 1107 9 -0.7054401212467631 1107 10-11 -0.10937525569547546 1107 12-13 -1.3959330877063785 1107 14-15 -1.6118763488140289 1107 16-17 -0.565615676502631 1107 18-19 -1.1730676409113805 1107 20-21 -0.632232379752498 1107 22-23 0.22033316714178497 1107 24-25 -1.414979731703717 1107 26-27 -2.234038776564585 1107 28-29 -0.22798496688196224 1107 30-31 -0.6253810298033855 1107 32-33 -1.429687020271352 1107 34-35 -1.5886065852446798 1107 36-37 -1.3988913569949872 1107 38-39 -2.1993488162023205 1107 40-41 -2.266733171601601 1107 42-43 -1.0861478712419554 1107 44-45 0.7827217621314837 1107 46-47 -0.28729335331090056 1107 48-49 -2.3171617135930553 1107 50-51 0.2426820451317333 1107 52-53 -0.9514925530841083 1107 54-55 -0.038208337630955214 1107 56-57 -0.4086521329367798 1107 58-59 -0.5068020115803336 1107 60-61 0.7606415206167867 1107 62-63 -0.3040576410539728 1107 64-65 0.8523524420608872 1107 66-67 -0.3632968742781948 1107 68-69 -1.9214464698012534 1107 70-71 -0.613203469085871 1107 72-73 -0.7571854468674672 1107 74-75 1.4938963219767345 1107 76 -1.9009310240712658 1108 1 1.2146645180499505 1108 2 -1.9152273889891127 1108 3 -1.5293299286043016 1108 4 0.3549201566098361 1108 5 -1.5752736368874132 1108 6 -1.1322329611784276 1108 7 -0.4272690414840419 1108 8 -0.5662820549930707 1108 9 0.9931485884306568 1108 10-11 0.8084674862400085 1108 12-13 -1.7830298618999265 1108 14-15 -0.25149328429790074 1108 16-17 -0.14474874101875912 1108 18-19 1.5209847784434594 1108 20-21 -0.27638560555895 1108 22-23 0.16640171552888106 1108 24-25 -0.3746571510585568 1108 26-27 -0.7542000668871651 1108 28-29 -0.21790432172067398 1108 30-31 0.7864133250353262 1108 32-33 1.831905721664132 1108 34-35 -0.8517114239543595 1108 36-37 -0.16754055054337513 1108 38-39 -0.5526754291055447 1108 40-41 -1.706753332891921 1108 42-43 -1.2499583551129234 1108 44-45 -0.6532661410943241 1108 46-47 -1.9012046436334842 1108 48-49 1.8159028025359767 1108 50-51 2.0829038193252813 1108 52-53 -0.5759885208260442 1108 54-55 0.03336424301420493 1108 56-57 -0.8411118103561321 1108 58-59 1.0294883110003141 1108 60-61 -0.6133504148670852 1108 62-63 -0.14528747976365253 1108 64-65 0.1386427646415349 1108 66-67 0.7969853837863212 1108 68-69 -0.21378517947866982 1108 70-71 -0.699392985214903 1108 72-73 0.9121693918422125 1108 74-75 -1.4012649683458456 1108 76 -0.7366165079422338 1109 1 2.2642036885568615 1109 2 -0.8818172507402657 1109 3 0.353158550658371 1109 4 0.7639063317250425 1109 5 -1.8713565861961676 1109 6 -0.4352283528834988 1109 7 0.5923162119721788 1109 8 1.211367714592182 1109 9 1.1383098787532369 1109 10-11 0.38392831573309394 1109 12-13 0.08620976943647918 1109 14-15 3.0555343654716864 1109 16-17 2.9410807520687996 1109 18-19 2.579164501945762 1109 20-21 -1.0027680940382133 1109 22-23 -0.732791832858215 1109 24-25 2.7457345540105678 1109 26-27 5.033818366292561 1109 28-29 -0.04048496688196224 1109 30-31 1.8768511130537568 1109 32-33 1.1573665511572173 1109 34-35 2.9069291290410355 1109 36-37 3.322090785862155 1109 38-39 1.5573476123691101 1109 40-41 1.9810346855412568 1109 42-43 3.1526914144723293 1109 44-45 2.119775333560053 1109 46-47 -0.006043353310900557 1109 48-49 0.9685525721212294 1109 50-51 3.052949902274591 1109 52-53 2.3900253040587494 1109 54-55 0.7787559480833295 1109 56-57 2.99759786706322 1109 58-59 1.4931979884196664 1109 60-61 1.247248663473929 1109 62-63 0.4526387875174578 1109 64-65 0.5487810134894566 1109 66-67 1.0608102685789476 1109 68-69 -0.001803612658399345 1109 70-71 -0.15561418337158628 1109 72-73 3.4213859817039634 1109 74-75 -1.5061036780232655 1109 76 0.7553189759287342 1110 1 -1.6286534543002809 1110 2 0.33246846354544957 1110 3 1.603158550658371 1110 4 -0.5218079539892422 1110 5 0.2715005566609747 1110 6 -1.6852283528834988 1110 7 0.378030497686467 1110 8 -1.288632285407818 1110 9 1.0311670216103792 1110 10-11 -0.09821454140976016 1110 12-13 0.9433526265793368 1110 14-15 1.7341057940431135 1110 16-17 -0.2910621050740616 1110 18-19 0.3470216448029042 1110 20-21 0.836517620247502 1110 22-23 1.6779224528560697 1110 24-25 0.24573455401056776 1110 26-27 -0.0376102051360121 1110 28-29 -0.9690563954533928 1110 30-31 -0.6767203155176702 1110 32-33 0.19308083687150557 1110 34-35 -0.7894994423875374 1110 36-37 -0.48148064270927193 1110 38-39 0.5037761837976795 1110 40-41 -0.2868224573158855 1110 42-43 0.4741199859009022 1110 44-45 -0.09451038072565865 1110 46-47 0.15467093240338414 1110 48-49 0.8614097149783753 1110 50-51 0.7136641879888792 1110 52-53 -0.9849746959412506 1110 54-55 0.10018451951189888 1110 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-0.5037104188317514 2116 64-65 -0.46709200238355564 2116 66-67 -0.9153802076115269 2116 68-69 -1.7478353586901427 2116 70-71 0.11422708646968616 2116 72-73 -3.1460743357563565 2116 74-75 1.1327852108656238 2116 76 0.9497634203731771 2117 1 2.1481322599854344 2117 2 0.18961132068830722 2117 3 4.129944264944086 2117 4 -1.1914508111320998 2117 5 1.9590005566609747 2117 6 6.3415573614022165 2117 7 3.494101926257894 2117 8 -0.5743465711221027 2117 9 -0.7991901212467631 2117 10-11 -2.5569547545956084E-7 2117 12-13 0.5103169122936215 2117 14-15 0.7162486511859711 2117 16-17 1.418759323497369 2117 18-19 -0.4074426409113805 2117 20-21 0.586517620247502 2117 22-23 1.860958167141785 2117 24-25 2.741270268296283 2117 26-27 -0.577788776564585 2117 28-29 2.3345150331180378 2117 30-31 0.8589939701966145 2117 32-33 -0.07031202027135208 2117 34-35 0.06764341475532021 2117 36-37 -1.1957663569949872 2117 38-39 0.3475261837976795 2117 40-41 0.4363918283983992 2117 42-43 -1.5080228712419554 2117 44-45 4.329596762131484 2117 46-47 3.5877066466890994 2117 48-49 1.3703382864069447 2117 50-51 -0.6166929548682667 2117 52-53 -0.1233675530841083 2117 54-55 4.836791662369045 2117 56-57 2.77884786706322 2117 58-59 0.27444798841966644 2117 60-61 3.9168915206167867 2117 62-63 2.711567358946027 2117 64-65 1.5086024420608872 2117 66-67 6.542953125721805 2117 68-69 3.0785535301987466 2117 70-71 4.589921530914129 2117 72-73 3.3678145531325328 2117 74-75 1.4938963219767345 2117 76 3.067818975928734 2118 1 1.4014217336696433 2118 2 -0.4024939424695866 2118 3 -0.9062399455822288 2118 4 4.081575504657373 2118 5 2.784658451397817 2118 6 -0.0762057964925198 2118 7 -2.2065559684789484 2118 8 -1.455925518490524 2118 9 1.269888826121658 2118 10-11 1.253289217988737 2118 12-13 1.419856385977834 2118 14-15 -1.8890145067087651 2118 16-17 0.3348777445499991 2118 18-19 -1.6360610619640106 2118 20-21 0.5470439360369745 2118 22-23 -1.7920023591740026 2118 24-25 3.2297571104015432 2118 26-27 1.1014875392248875 2118 28-29 2.0121466120654077 2118 30-31 2.1879413386176694 2118 32-33 -0.3811672834292459 2118 34-35 -0.6955144799815223 2118 36-37 -0.5181347780476173 2118 38-39 -1.5883290793602143 2118 40-41 -1.4155818558121283 2118 42-43 -0.9290755028209006 2118 44-45 -1.7806006062895676 2118 46-47 -3.2297275638372156 2118 48-49 0.13678565482799954 2118 50-51 0.1958070451317333 2118 52-53 -1.3815912372946357 2118 54-55 0.3483048202637846 2118 56-57 -0.02871792241046478 2118 58-59 0.8155664094722965 2118 60-61 0.696496783774684 2118 62-63 -2.4890905357908153 2118 64-65 -1.6805422947812154 2118 66-67 1.9837425994060176 2118 68-69 0.14105353019874656 2118 70-71 0.4007767940720228 2118 72-73 -0.875606499499046 2118 74-75 0.13863316408199822 2118 76 -3.15257576091337 2119 1 -2.4143677400145656 2119 2 3.725325606402592 2119 3 -0.21827002077019841 2119 4 2.0853349031536155 2119 5 1.3786434138038324 2119 6 -5.328085495740641 2119 7 -5.586255216599248 2119 8 -3.6100608568363874 2119 9 -0.07597583553247844 2119 10-11 -1.669643112838333 2119 12-13 -2.324504516277809 2119 14-15 0.03767722261454054 2119 16-17 -1.5410621050740616 2119 18-19 1.4541645019457619 2119 20-21 0.9079461916760714 2119 22-23 -3.0720775471439303 2119 24-25 -1.2364083031322863 2119 26-27 0.2123897948639879 2119 28-29 -0.36191353831053164 2119 30-31 -1.7838631726605279 2119 32-33 -1.9676334488427827 2119 34-35 -1.646642299530395 2119 36-37 -0.24933778556641428 2119 38-39 -1.4962238162023205 2119 40-41 0.08817754268411448 2119 42-43 -2.150880014099098 2119 44-45 -2.1659389521542316 2119 46-47 -1.6846147818823312 2119 48-49 0.1649811435498023 2119 50-51 -1.3041929548682667 2119 52-53 -0.7706889816555389 2119 54-55 -2.185529766202386 2119 56-57 -2.3595449900796375 2119 58-59 -2.1675162972946183 2119 60-61 -2.324179907954644 2119 62-63 1.3454959303745966 2119 64-65 1.513066727775172 2119 66-67 -2.6534754457067677 2119 68-69 -1.1089464698012534 2119 70-71 -1.1556141833715863 2119 72-73 -1.3286140182960402 2119 74-75 -2.79181796373755 2119 76 -4.208966738356979 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 3.0 12 7.0 13 67.0 14 257.0 15 630.0 16 981.0 17 1222.0 18 1284.0 19 1253.0 20 1104.0 21 838.0 22 557.0 23 340.0 24 208.0 25 136.0 26 95.0 27 72.0 28 49.0 29 42.0 30 37.0 31 29.0 32 16.0 33 18.0 34 14.0 35 24.0 36 31.0 37 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.311827956989248 29.118279569892476 8.526881720430108 56.04301075268817 2 2.654109589041096 46.32919520547945 22.527825342465754 28.4888698630137 3 3.2106164383561646 29.22731164383562 19.424229452054796 48.13784246575342 4 2.2902397260273974 25.61001712328767 17.305222602739725 54.794520547945204 5 2.0012842465753424 51.59460616438356 16.716609589041095 29.6875 6 3.617294520547945 31.945633561643838 7.983732876712329 56.453339041095894 7 3.125 50.8347602739726 8.625856164383562 37.41438356164384 8 3.413955479452055 35.85188356164384 10.466609589041095 50.267551369863014 9 7.59845890410959 32.47003424657534 9.00042808219178 50.93107876712328 10-11 5.67208904109589 32.65732020547945 21.69306506849315 39.97752568493151 12-13 5.789811643835616 28.413955479452053 22.07298801369863 43.7232448630137 14-15 10.782320205479452 31.86536815068493 17.792166095890412 39.56014554794521 16-17 15.571489726027396 31.48544520547945 17.080479452054796 35.86258561643836 18-19 11.403039383561644 28.804580479452053 15.598244863013699 44.1941352739726 20-21 8.850599315068493 29.40924657534247 18.263056506849313 43.477097602739725 22-23 17.883133561643834 29.51626712328767 14.142765410958905 38.45783390410959 24-25 13.035102739726026 35.825128424657535 13.752140410958905 37.387628424657535 26-27 9.728167808219178 36.53681506849315 19.17808219178082 34.55693493150685 28-29 14.245197195911597 30.507839674640124 23.074864879327876 32.172098250120406 30-31 13.47388698630137 30.26005993150685 24.732448630136986 31.533604452054792 32-33 9.610445205479452 37.17893835616438 21.92315924657534 31.28745719178082 34-35 9.118150684931507 35.91609589041096 20.69777397260274 34.26797945205479 36-37 11.263912671232877 29.53767123287671 23.23951198630137 35.95890410958904 38-39 11.296018835616438 24.5826198630137 27.66481164383562 36.45654965753425 40-41 8.759631849315069 26.62671232876712 25.26755136986301 39.34610445205479 42-43 4.751712328767123 25.294306506849317 29.61258561643836 40.34139554794521 44-45 6.603167808219178 27.95376712328767 30.98779965753425 34.4552654109589 46-47 6.790453767123288 32.25599315068493 25.722388698630137 35.23116438356164 48-49 6.945633561643835 35.13484589041096 19.48844178082192 38.43107876712329 50-51 6.490796232876712 32.43792808219178 19.36001712328767 41.71125856164384 52-53 6.132277397260274 31.82255993150685 21.28638698630137 40.75877568493151 54-55 6.287457191780822 31.46939212328767 20.13056506849315 42.11258561643836 56-57 10.696703767123287 31.496147260273972 18.091823630136986 39.71532534246575 58-59 9.74422089041096 30.26005993150685 18.20419520547945 41.79152397260274 60-61 6.752996575342466 27.50963184931507 19.71318493150685 46.02418664383562 62-63 9.647902397260275 29.48951198630137 17.647688356164384 43.21489726027397 64-65 9.920804794520548 31.05736301369863 13.971532534246576 45.05029965753425 66-67 7.04195205479452 37.33946917808219 13.136772260273974 42.48180650684932 68-69 10.038527397260275 36.06592465753425 13.12071917808219 40.77482876712329 70-71 8.518835616438356 36.54216609589041 14.169520547945206 40.76947773972603 72-73 8.930864726027398 37.24315068493151 12.13077910958904 41.69520547945205 74-75 10.113441780821917 36.69734589041096 11.943493150684931 41.24571917808219 76 8.261986301369863 39.12671232876712 12.275256849315069 40.33604452054795 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 0.5 14 0.5 15 3.0 16 3.5 17 2.0 18 4.0 19 6.5 20 8.5 21 10.0 22 12.5 23 12.0 24 15.0 25 21.0 26 24.5 27 36.5 28 42.0 29 39.0 30 43.5 31 62.0 32 89.5 33 103.0 34 110.0 35 134.0 36 168.0 37 185.0 38 228.0 39 278.5 40 329.0 41 392.5 42 413.0 43 464.5 44 534.5 45 531.0 46 509.0 47 531.5 48 564.0 49 584.0 50 594.0 51 584.0 52 541.5 53 501.0 54 493.0 55 462.5 56 389.5 57 322.5 58 298.0 59 284.0 60 239.5 61 188.5 62 168.0 63 138.0 64 102.0 65 83.0 66 75.5 67 81.0 68 59.5 69 33.5 70 27.5 71 26.0 72 20.0 73 10.5 74 8.5 75 10.0 76 10.0 77 8.0 78 5.0 79 4.0 80 2.5 81 1.5 82 2.0 83 2.0 84 3.5 85 4.0 86 3.0 87 3.0 88 2.0 89 1.0 90 2.0 91 2.5 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 1.0 98 0.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4708904109589041 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.005351027397260274 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 9344.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.65753424657534 #Duplication Level Percentage of deduplicated Percentage of total 1 99.75300687285224 99.41138698630137 2 0.19329896907216496 0.3852739726027397 3 0.03221649484536082 0.09631849315068493 4 0.010738831615120275 0.04280821917808219 5 0.0 0.0 6 0.010738831615120275 0.06421232876712328 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.010702054794520547 0.0 17 0.0 0.0 0.0 0.010702054794520547 0.0 18 0.0 0.0 0.0 0.021404109589041095 0.0 19 0.0 0.0 0.0 0.021404109589041095 0.0 20 0.0 0.0 0.0 0.021404109589041095 0.0 21 0.0 0.0 0.0 0.021404109589041095 0.0 22 0.0 0.0 0.0 0.021404109589041095 0.0 23 0.0 0.0 0.0 0.03210616438356165 0.0 24 0.0 0.0 0.0 0.04280821917808219 0.0 25 0.0 0.0 0.0 0.04280821917808219 0.0 26 0.0 0.0 0.0 0.04280821917808219 0.0 27 0.0 0.0 0.0 0.04280821917808219 0.0 28 0.0 0.0 0.0 0.04280821917808219 0.0 29 0.0 0.0 0.0 0.04280821917808219 0.0 30 0.0 0.0 0.0 0.04280821917808219 0.0 31 0.0 0.0 0.0 0.05351027397260274 0.0 32 0.0 0.0 0.0 0.05351027397260274 0.0 33 0.0 0.0 0.0 0.05351027397260274 0.0 34 0.0 0.0 0.0 0.07491438356164383 0.0 35 0.0 0.0 0.0 0.08561643835616438 0.0 36 0.0 0.0 0.0 0.09631849315068493 0.0 37 0.0 0.0 0.0 0.14982876712328766 0.0 38 0.0 0.0 0.0 0.19263698630136986 0.0 39 0.0 0.0 0.0 0.2782534246575342 0.0 40 0.0 0.0 0.0 0.2889554794520548 0.0 41 0.0 0.0 0.0 0.3424657534246575 0.0 42 0.0 0.0 0.0 0.4066780821917808 0.0 43 0.0 0.0 0.0 0.4708904109589041 0.0 44 0.0 0.0 0.0 0.5351027397260274 0.0 45 0.0 0.0 0.0 0.5779109589041096 0.0 46 0.0 0.0 0.0 0.6100171232876712 0.0 47 0.0 0.0 0.0 0.6421232876712328 0.0 48 0.0 0.0 0.0 0.7063356164383562 0.0 49 0.0 0.0 0.0 0.7491438356164384 0.0 50 0.0 0.0 0.0 0.759845890410959 0.0 51 0.0 0.0 0.0 0.802654109589041 0.0 52 0.0 0.0 0.0 0.8347602739726028 0.0 53 0.0 0.0 0.0 0.877568493150685 0.0 54 0.0 0.0 0.0 0.9310787671232876 0.0 55 0.0 0.0 0.0 0.9524828767123288 0.0 56 0.0 0.0 0.0 0.9738869863013698 0.0 57 0.0 0.0 0.0 0.995291095890411 0.0 58 0.0 0.0 0.0 1.0380993150684932 0.0 59 0.0 0.0 0.0 1.0595034246575343 0.0 60 0.0 0.0 0.0 1.0702054794520548 0.0 61 0.0 0.0 0.0 1.1237157534246576 0.0 62 0.0 0.0 0.0 1.134417808219178 0.0 63 0.0 0.0 0.0 1.1665239726027397 0.0 64 0.0 0.0 0.0 1.1986301369863013 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE