FastQCFastQC Report
Wed 25 May 2016
SRR1780364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9344
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT222423.8013698630137No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT188020.11986301369863TruSeq Adapter, Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC6136.560359589041095No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG3013.221318493150685No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC2622.803938356164384No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT1601.7123287671232876No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT1291.3805650684931507No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG770.8240582191780822No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC760.8133561643835616No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT760.8133561643835616No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT730.78125No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC650.6956335616438356No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT650.6956335616438356No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG650.6956335616438356No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT580.6207191780821918No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC520.5565068493150684No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT510.545804794520548No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC480.5136986301369862No Hit
TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC340.3638698630136986No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCG320.3424657534246575No Hit
CTTATACACATCTCCGAGCCCACGCGACGTGTGGTGATCTCGTATGCCGT320.3424657534246575No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTGTGGTGATCTCGTATGCCGT320.3424657534246575No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCG290.3103595890410959No Hit
CTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT280.2996575342465753No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGCTCTCGTATGCCGTCTT270.2889554794520548No Hit
ATACACATCTCCGAGCCCACGAGAAGTGTGGTGATCTCGTATGCCGTCTT230.2461472602739726Illumina PCR Primer Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTC230.2461472602739726No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCG220.23544520547945205No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGT210.2247431506849315No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTC210.2247431506849315No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG210.2247431506849315No Hit
CTTATACACATCTCCGAGCACACGAGACGTGTGGTGATCTCGTATGCCGT200.21404109589041095No Hit
CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC190.2033390410958904No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTGGTGATCTCGTATGCCGT180.19263698630136986No Hit
ATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGTCTT160.17123287671232876Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGAAGTGTGGTGATCTCGTATGCCGT160.17123287671232876No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCAGT150.1605308219178082No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCAGTCTT150.1605308219178082No Hit
TCTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT150.1605308219178082No Hit
ATACACATCTCCGAGCCCACGAGACGAGTGGTGATCTCGTATGCCGTCTT150.1605308219178082TruSeq Adapter, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTGAGGTGATCTCGTATGCCGTCTT140.14982876712328766TruSeq Adapter, Index 5 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG140.14982876712328766No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTAGTATGCCGT140.14982876712328766No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTCTGCCGTCTT140.14982876712328766No Hit
ACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCT140.14982876712328766RNA PCR Primer, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCACGTATGCCGT140.14982876712328766No Hit
TCTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT130.1391267123287671No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAAATCGTATGCCGT130.1391267123287671No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG120.12842465753424656No Hit
ATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTGCTT120.12842465753424656TruSeq Adapter, Index 5 (96% over 27bp)
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTC120.12842465753424656No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAGCTCGTATGCCGTCTT110.11772260273972603No Hit
CTTACACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT110.11772260273972603No Hit
CTTATACACATATCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT110.11772260273972603No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT100.10702054794520548No Hit
ATACACATCTACGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT100.10702054794520548TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACATCGTATGCCGT100.10702054794520548No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGCTCTCGTATGCCGT100.10702054794520548No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGAGATCTCGTATGCCGTCTT100.10702054794520548RNA PCR Primer, Index 17 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCCT100.10702054794520548No Hit
CTTATACACATCTCCGAGCCCACGTGACGTGTGGTGATCTCGTATGCCGT100.10702054794520548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA150.00215596870.01
CTTTACA1150.070.01
ACACACA150.00215596870.070
ACGAGTG150.00215596870.027
CTTATCA150.00215596870.01
GACGAGT150.00215596870.026
TCTTTAT150.00215596870.01
TTTACAC1150.070.02
TTACACA1200.067.083333
TCTTATA1000.055.9999961
CTTATAC4400.054.8863681
CACAAAA200.006738255352.569
TTATACA4700.051.382982
TATACAC5050.047.821783
GGTGATA503.122319E-642.033
GATATCG503.122319E-642.036
TATCGTA503.122319E-642.038
TGATATC503.122319E-642.035
GTGATAT503.122319E-642.034
ATATCGT503.122319E-642.037
Produced by FastQC (version 0.11.5)