##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780364_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9344 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70237585616438 34.0 34.0 34.0 31.0 34.0 2 33.39736729452055 34.0 34.0 34.0 33.0 34.0 3 33.51990582191781 34.0 34.0 34.0 34.0 34.0 4 33.493364726027394 34.0 34.0 34.0 34.0 34.0 5 33.55532962328767 34.0 34.0 34.0 34.0 34.0 6 37.36750856164384 38.0 38.0 38.0 37.0 38.0 7 36.88452482876713 38.0 37.0 38.0 35.0 38.0 8 37.029965753424655 38.0 37.0 38.0 36.0 38.0 9 36.64736729452055 38.0 37.0 38.0 34.0 38.0 10-11 36.382705479452056 38.0 37.0 38.0 34.0 38.0 12-13 36.052707619863014 38.0 36.5 38.0 33.0 38.0 14-15 35.94563356164383 38.0 36.0 38.0 31.5 38.0 16-17 35.92888484589041 38.0 36.0 38.0 32.0 38.0 18-19 36.10894691780822 38.0 36.5 38.0 32.5 38.0 20-21 35.88195633561644 38.0 36.0 38.0 31.0 38.0 22-23 35.775417380136986 38.0 36.0 38.0 32.0 38.0 24-25 34.27381207191781 37.0 33.5 38.0 27.0 38.0 26-27 33.99186643835617 37.0 34.0 38.0 25.5 38.0 28-29 34.023330479452056 37.0 34.0 38.0 25.0 38.0 30-31 33.13195633561644 36.5 32.0 38.0 22.0 38.0 32-33 34.21281035958904 37.0 34.0 38.0 26.5 38.0 34-35 33.00786601027397 36.5 32.5 38.0 18.0 38.0 36-37 32.78954409246575 37.0 33.0 38.0 21.0 38.0 38-39 30.32919520547945 34.0 27.0 37.0 11.0 38.0 40-41 32.75502996575342 36.5 32.5 37.0 17.5 38.0 42-43 34.66154751712329 37.0 34.0 38.0 28.5 38.0 44-45 35.17358732876713 37.0 35.5 38.0 29.0 38.0 46-47 35.12778253424658 37.0 35.5 38.0 29.0 38.0 48-49 34.608839897260275 37.0 34.5 38.0 28.0 38.0 50-51 34.46789383561644 37.0 34.0 38.0 26.0 38.0 52-53 34.21527183219178 37.0 34.0 38.0 24.5 38.0 54-55 34.67123287671233 37.0 34.5 38.0 27.0 38.0 56-57 34.55747003424658 37.0 34.0 38.0 27.0 38.0 58-59 34.13677226027397 37.0 34.0 38.0 24.0 38.0 60-61 34.13527397260274 37.0 34.0 38.0 25.0 38.0 62-63 34.977739726027394 37.0 35.5 38.0 27.0 38.0 64-65 34.94734589041096 37.0 35.0 38.0 29.0 38.0 66-67 32.570151969178085 36.5 31.0 38.0 21.0 38.0 68-69 28.155607876712327 32.5 21.5 37.0 11.0 38.0 70-71 23.841770119863014 25.0 11.0 33.5 10.5 37.0 72-73 19.78162457191781 20.0 11.0 25.0 10.5 34.0 74-75 19.112371575342465 19.0 11.0 25.0 11.0 34.0 76 19.36611729452055 19.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0845865980177223 1101 2 0.4753292682529988 1101 3 0.37511512445983186 1101 4 0.45711828740917326 1101 5 0.4519559382299363 1101 6 -0.2347517458758972 1101 7 0.45609742953805466 1101 8 0.5013978893048332 1101 9 -0.926411739343564 1101 10-11 0.2690641296000251 1101 12-13 0.9711550710877432 1101 14-15 0.7423769119571588 1101 16-17 0.11712387266631197 1101 18-19 -0.6172544634404247 1101 20-21 0.174515669177552 1101 22-23 0.8828941007958448 1101 24-25 -2.197099024693628 1101 26-27 0.406212202355583 1101 28-29 -0.06873702137349369 1101 30-31 1.49441500045603 1101 32-33 1.6941173957410172 1101 34-35 1.2599117686522163 1101 36-37 0.1881732479144489 1101 38-39 0.8518789964465512 1101 40-41 0.6041582247657828 1101 42-43 -0.041113572193197 1101 44-45 -0.2770595900456314 1101 46-47 0.6702924931033323 1101 48-49 -2.9297837599496965 1101 50-51 -1.320840250166313 1101 52-53 -2.48685987507017 1101 54-55 -2.9852725352573017 1101 56-57 -5.159147647529771 1101 58-59 -2.7037315370689115 1101 60-61 -0.7701844357649179 1101 62-63 -0.6797352716574778 1101 64-65 -0.634219144677246 1101 66-67 -1.3976178390627219 1101 68-69 -1.9673913452492684 1101 70-71 -3.6600234388393 1101 72-73 1.20438727420985 1101 74-75 1.8325917814797243 1101 76 3.9755983014557508 1102 1 -1.3888433536730993 1102 2 0.36904907501628514 1102 3 0.5232632726079771 1102 4 0.4377946159115922 1102 5 0.4519559382299363 1102 6 0.49310654719978686 1102 7 -1.068862312812989 1102 8 0.3870661662774566 1102 9 0.9721389852941229 1102 10-11 -0.4008231489829086 1102 12-13 0.5846816411360578 1102 14-15 -0.6110852458528271 1102 16-17 -0.8377875605059941 1102 18-19 -0.8467230624742399 1102 20-21 1.2453691313353659 1102 22-23 0.7500438592499492 1102 24-25 -6.721253614065606 1102 26-27 -4.874786187338458 1102 28-29 -0.653278084175426 1102 30-31 0.7150269167201202 1102 32-33 -0.7624043433894201 1102 34-35 -1.007399020397699 1102 36-37 -4.422937863196665 1102 38-39 -1.6714704399463614 1102 40-41 2.0123707851522568 1102 42-43 1.038596572734349 1102 44-45 1.4049049832232896 1102 46-47 2.29589635783762 1102 48-49 -4.161667817920712 1102 50-51 -1.4255101374449026 1102 52-53 -1.1865378138785374 1102 54-55 1.048543889863474 1102 56-57 0.3642017888631415 1102 58-59 -0.8172581071172189 1102 60-61 -6.7122134212721605 1102 62-63 -4.091168443960214 1102 64-65 -1.1366346036144463 1102 66-67 0.10640792583261316 1102 68-69 -3.4335749201284926 1102 70-71 -1.5923905885977554 1102 72-73 -1.1667882491395858 1102 74-75 -2.6778752072481353 1102 76 0.2541812322125949 1103 1 1.135380248811373 1103 2 0.5578689507926811 1103 3 0.23754898689369242 1103 4 0.5396579699488555 1103 5 0.25195593822993345 1103 6 0.3800630689389166 1103 7 0.8603302337708598 1103 8 0.5088052967122394 1103 9 0.8069215939897703 1103 10-11 0.16377312430901725 1103 12-13 -0.29233699240432287 1103 14-15 0.0783557479359942 1103 16-17 1.1150074705499051 1103 18-19 0.8986185524325876 1103 20-21 -0.6471774525155638 1103 22-23 0.400354418256164 1103 24-25 -4.35106727866188 1103 26-27 0.4183815145248957 1103 28-29 1.4709455183090512 1103 30-31 2.8187536247946525 1103 32-33 3.3226888243124506 1103 34-35 1.7112345199749655 1103 36-37 -0.45150929176809385 1103 38-39 1.1682811128486676 1103 40-41 0.9819360025435628 1103 42-43 0.6112673801877548 1103 44-45 1.983786970800928 1103 46-47 1.7337845565953884 1103 48-49 -1.7535932837592227 1103 50-51 0.8881544588283958 1103 52-53 2.132716844506554 1103 54-55 1.4274258774411166 1103 56-57 1.3815930932109666 1103 58-59 1.602617669280292 1103 60-61 -3.7463749119553924 1103 62-63 -0.010423102345306745 1103 64-65 1.7689554584973592 1103 66-67 -0.8600517014965874 1103 68-69 -0.668978646836571 1103 70-71 -0.4346266134424752 1103 72-73 -0.17921060938803635 1103 74-75 -1.4822230333350923 1103 76 0.209460735318185 1104 1 0.4401421535732766 1104 2 0.2864403793641159 1104 3 0.28992993927464283 1104 4 -0.1317706014797153 1104 5 -0.014710728436732268 1104 6 0.2800630689389223 1104 7 0.3079492813899094 1104 8 0.342138630045568 1104 9 1.3402549273231017 1104 10-11 0.3875826481185385 1104 12-13 0.6100439599766361 1104 14-15 1.1997843193645608 1104 16-17 0.7078646134070539 1104 18-19 0.9438566476706853 1104 20-21 0.18377492843681154 1104 22-23 0.22178298968473342 1104 24-25 0.40845653086193323 1104 26-27 1.9636196097629934 1104 28-29 2.7923740897376206 1104 30-31 0.5758964819375123 1104 32-33 1.1774507290743514 1104 34-35 0.48028213902258443 1104 36-37 2.1937288034700018 1104 38-39 2.6944715890391393 1104 40-41 3.5486026692102257 1104 42-43 1.52555309447348 1104 44-45 1.1266441136580738 1104 46-47 1.8480702708811094 1104 48-49 0.8202162400502999 1104 50-51 1.381011601685536 1104 52-53 1.594621606411316 1104 54-55 1.7036163536315883 1104 56-57 1.731593093210968 1104 58-59 0.2240462407088657 1104 60-61 1.2798155642350864 1104 62-63 1.3276721357499284 1104 64-65 1.1880030775449697 1104 66-67 0.18756734612246362 1104 68-69 0.5381642103062916 1104 70-71 0.3844210056051445 1104 72-73 -0.719686799864224 1104 74-75 2.617776966664909 1104 76 2.5904131162705646 1105 1 1.3068088202399437 1105 2 0.5864403793641131 1105 3 0.5232632726079771 1105 4 0.5682293985202875 1105 5 0.018622604896606276 1105 6 0.1133964022722509 1105 7 0.8746159480565723 1105 8 -0.057861369954430586 1105 9 1.2735882606564388 1105 10-11 0.5042493147852056 1105 12-13 -1.573289373356701 1105 14-15 0.9831176526978922 1105 16-17 1.3411979467403867 1105 18-19 0.8438566476706839 1105 20-21 1.0004415951034815 1105 22-23 1.6051163230180663 1105 24-25 2.5917898641952704 1105 26-27 1.863619609762992 1105 28-29 1.5090407564042891 1105 30-31 2.092563148604178 1105 32-33 2.6274507290743543 1105 34-35 -0.7863845276440777 1105 36-37 2.560395470136669 1105 38-39 1.2444715890391436 1105 40-41 1.96526933587689 1105 42-43 1.1422197611401401 1105 44-45 2.176644113658071 1105 46-47 2.5147369375477666 1105 48-49 0.9035495733836356 1105 50-51 1.381011601685529 1105 52-53 1.8779549397446473 1105 54-55 2.470283020298261 1105 56-57 1.4982597598776337 1105 58-59 2.4740462407088657 1105 60-61 2.7631488975684206 1105 62-63 1.411005469083257 1105 64-65 1.8880030775449796 1105 66-67 3.287567346122458 1105 68-69 1.5548308769729573 1105 70-71 1.9677543389384766 1105 72-73 3.4803132001357753 1105 74-75 1.6677769666649063 1105 76 -1.776253550396099 1106 1 -1.6931911797600563 1106 2 0.2664403793641128 1106 3 0.12326327260797854 1106 4 -1.1917706014797105 1106 5 0.09195593822993686 1106 6 0.3000630689389183 1106 7 -1.8253840519434235 1106 8 0.14880529671223997 1106 9 -1.51307840601023 1106 10-11 9.159814518682197E-4 1106 12-13 1.533377293309968 1106 14-15 -1.3302156806354404 1106 16-17 -0.14213538659294755 1106 18-19 0.08718998100402331 1106 20-21 0.4271082617701438 1106 22-23 0.4517829896847303 1106 24-25 -0.10821013580473249 1106 26-27 1.2469529430963249 1106 28-29 1.882374089737624 1106 30-31 1.8758964819375095 1106 32-33 1.8741173957410169 1106 34-35 1.2569488056892482 1106 36-37 1.8570621368033358 1106 38-39 1.3311382557058096 1106 40-41 1.9219360025435606 1106 42-43 0.525553094473473 1106 44-45 0.906644113658075 1106 46-47 1.1880702708811057 1106 48-49 1.763549573383635 1106 50-51 0.7910116016855397 1106 52-53 1.1812882730779748 1106 54-55 -0.6297169797017474 1106 56-57 1.8615930932109706 1106 58-59 2.4140462407088705 1106 60-61 2.3964822309017535 1106 62-63 1.421005469083262 1106 64-65 -2.9453302557883596 1106 66-67 -0.18576598721087123 1106 68-69 0.4281642103062886 1106 70-71 -0.8289123277281902 1106 72-73 1.7236465334691111 1106 74-75 -1.9822230333350923 1106 76 0.8837464496039011 1107 1 1.0880588202399437 1107 2 0.3989403793641131 1107 3 0.21076327260797711 1107 4 0.22447939852028753 1107 5 0.014455938229936294 1107 6 -0.013686931061080543 1107 7 0.8808659480565737 1107 8 0.12755529671223798 1107 9 -1.0930784060102283 1107 10-11 0.9334159814518728 1107 12-13 0.8496272933099647 1107 14-15 0.9622843193645636 1107 16-17 1.7484896134070524 1107 18-19 0.8896899810040182 1107 20-21 1.5327332617701472 1107 22-23 -0.02509201031526942 1107 24-25 0.7074148641952647 1107 26-27 1.3969529430963235 1107 28-29 1.2923740897376206 1107 30-31 2.616521481937511 1107 32-33 2.319117395741017 1107 34-35 0.9219488056892544 1107 36-37 2.8895621368033346 1107 38-39 0.5017632557058107 1107 40-41 0.19443600254356141 1107 42-43 1.9224280944734744 1107 44-45 2.082894113658071 1107 46-47 1.9324452708811037 1107 48-49 1.4660495733836356 1107 50-51 2.0466366016855346 1107 52-53 1.9269132730779788 1107 54-55 0.9452830202982554 1107 56-57 1.5972180932109694 1107 58-59 1.8240462407088671 1107 60-61 2.1308572309017535 1107 62-63 1.7328804690832627 1107 64-65 1.7546697442116397 1107 66-67 -0.030140987210870662 1107 68-69 3.4006642103062887 1107 70-71 1.310462672271811 1107 72-73 -0.13010346653089044 1107 74-75 -0.5603480333350923 1107 76 0.6549964496038996 1108 1 -0.6931911797600563 1108 2 0.4574081212995935 1108 3 -0.37996253384363854 1108 4 0.5359713340041594 1108 5 0.35518174468155195 1108 6 -0.03284015686752895 1108 7 -0.057642116459554416 1108 8 -0.34925921941679405 1108 9 -0.04469130923603615 1108 10-11 0.5563998524196165 1108 12-13 1.8224095513744771 1108 14-15 0.5207520613000511 1108 16-17 0.13205816179415564 1108 18-19 0.5529964326169221 1108 20-21 1.2832372940282113 1108 22-23 -1.2604750748313975 1108 24-25 1.6272737351630084 1108 26-27 -1.5465954440004452 1108 28-29 -1.2640775231656107 1108 30-31 1.508154546453639 1108 32-33 1.8392786860635937 1108 34-35 2.442110096011831 1108 36-37 1.6415782658355909 1108 38-39 2.5788801911896826 1108 40-41 1.736774712220985 1108 42-43 -0.6163823893974936 1108 44-45 1.2169666943032311 1108 46-47 1.770650916042399 1108 48-49 2.226130218544924 1108 50-51 1.869721279104894 1108 52-53 0.4999979504973311 1108 54-55 1.1509281815885757 1108 56-57 0.06546406095290536 1108 58-59 0.7514655955475718 1108 60-61 1.8319661018694973 1108 62-63 1.7868119206961666 1108 64-65 1.3030568409858319 1108 66-67 1.147782399885898 1108 68-69 2.4430029199837087 1108 70-71 0.483345736787939 1108 72-73 1.7055820173400775 1108 74-75 1.4210027731165233 1108 76 -0.06980193749287622 1109 1 -2.9074768940457716 1109 2 0.2650118079355437 1109 3 0.5232632726079771 1109 4 -0.36034203005114307 1109 5 -0.1194726331986331 1109 6 -0.5627940739182264 1109 7 -0.2753840519434263 1109 8 -0.12690898900204672 1109 9 0.4783501654183411 1109 10-11 0.7637731243090116 1109 12-13 0.5862344361671106 1109 14-15 0.9890700336502718 1109 16-17 0.9650074705499136 1109 18-19 0.5236185524325876 1109 20-21 1.8563939760558554 1109 22-23 0.8074972753990224 1109 24-25 -0.0046387072333047286 1109 26-27 1.2898100859534622 1109 28-29 -0.10048305311951822 1109 30-31 -0.2919606609196279 1109 32-33 1.9976888243124478 1109 34-35 0.9576630914035391 1109 36-37 3.5056335653747652 1109 38-39 1.753995398562953 1109 40-41 1.5962217168292767 1109 42-43 1.7505530944734744 1109 44-45 -0.0019273149133596235 1109 46-47 1.5016416994525343 1109 48-49 0.22497814481220502 1109 50-51 -1.1832741126001807 1109 52-53 -1.9815688697791671 1109 54-55 -0.2154312654160293 1109 56-57 0.6351645217824 1109 58-59 -1.7116680450054176 1109 60-61 0.7357679451874688 1109 62-63 -1.1711373880595914 1109 64-65 0.7546697442116397 1109 66-67 -1.1529088443537283 1109 68-69 -1.0904072182651419 1109 70-71 0.6653733865575262 1109 72-73 1.2493608191833943 1109 74-75 1.7499198238077618 1109 76 -1.4476821218246734 1110 1 0.1282373916685131 1110 2 0.4435832365069672 1110 3 0.416120415465123 1110 4 0.42537225566314163 1110 5 0.4519559382299363 1110 6 -0.5627940739182264 1110 7 0.7246159480565737 1110 8 0.4445195824265227 1110 9 1.228350165418341 1110 10-11 -0.9326554471195578 1110 12-13 1.014805864738534 1110 14-15 1.4354986050788483 1110 16-17 1.4650074705499065 1110 18-19 -0.5835243047102736 1110 20-21 1.159965404627286 1110 22-23 -0.9782170103152694 1110 24-25 -0.07606727866187413 1110 26-27 2.4326672288106153 1110 28-29 1.11380266116619 1110 30-31 1.083039339080372 1110 32-33 0.9798316814553019 1110 34-35 1.8148059485463932 1110 36-37 2.1127764225176193 1110 38-39 1.1647096842772413 1110 40-41 -1.6716354260278692 1110 42-43 0.804124523044905 1110 44-45 1.8909298279437863 1110 46-47 1.662355985166819 1110 48-49 1.8856924305264968 1110 50-51 -0.7011312554573266 1110 52-53 1.9112882730779788 1110 54-55 -1.4118598368446058 1110 56-57 1.5994502360681082 1110 58-59 0.7169033835660059 1110 60-61 1.5393393737588923 1110 62-63 -1.0461373880595985 1110 64-65 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1.0610054690832627 2116 64-65 -3.4675524780105818 2116 66-67 -0.68465487609976 2116 68-69 0.5603864325285102 2116 70-71 -1.3544678832837462 2116 72-73 -0.09190902208644758 2116 74-75 -2.2877785888906494 2116 76 -0.3762535503961004 2117 1 1.3068088202399437 2117 2 0.5864403793641131 2117 3 0.5232632726079771 2117 4 0.38072939852028753 2117 5 -0.4230440617700637 2117 6 0.20506306893891946 2117 7 -0.0878840519434263 2117 8 -0.278694703287762 2117 9 -2.3430784060102283 2117 10-11 -2.4103340185481272 2117 12-13 -1.9941227066900353 2117 14-15 0.2122843193645636 2117 16-17 -3.6421353865929476 2117 18-19 -2.422810018995982 2117 20-21 -0.6391417382298528 2117 22-23 -0.6032170103152694 2117 24-25 -1.6832101358047353 2117 26-27 -4.3842970569036765 2117 28-29 -3.7701259102623794 2117 30-31 -2.149103518062489 2117 32-33 -6.368382604258983 2117 34-35 -1.4530511943107456 2117 36-37 -3.6729378631966654 2117 38-39 -1.3888617442941893 2117 40-41 -1.4305639974564386 2117 42-43 -1.4056969055265256 2117 44-45 -0.292105886341929 2117 46-47 -1.3644297291188963 2117 48-49 -0.7527004266163644 2117 50-51 -0.25023839831446537 2117 52-53 -1.1199617269220212 2117 54-55 -0.2734669797017446 2117 56-57 -0.7309069067890306 2117 58-59 -0.8322037592911329 2117 60-61 -0.5410177690982465 2117 62-63 -1.7827445309167373 2117 64-65 -1.5890802557883603 2117 66-67 -2.6395159872108707 2117 68-69 0.49441421030628874 2117 70-71 1.857337672271811 2117 72-73 0.5886465334691096 2117 74-75 -1.3884730333350923 2117 76 -2.6262535503961004 2118 1 0.7804930307662588 2118 2 0.42854564252201044 2118 3 -2.529368306339393 2118 4 -1.0633495488481302 2118 5 -0.39014932492796106 2118 6 0.0011157005178645818 2118 7 0.18514226384604626 2118 8 -0.9332999664456594 2118 9 -5.014131037589177 2118 10-11 -0.8790840185481272 2118 12-13 -2.6224121803742406 2118 14-15 -0.24495252274069657 2118 16-17 -5.484240649750841 2118 18-19 -2.4359679137328243 2118 20-21 -0.9960496329666952 2118 22-23 0.03494088442157306 2118 24-25 0.45494775893210715 2118 26-27 -3.7740996884826217 2118 28-29 -2.5102574892097493 2118 30-31 -3.1556824654309104 2118 32-33 -1.3848299726800377 2118 34-35 -0.8083143522054854 2118 36-37 -3.9492536526703503 2118 38-39 -1.6783354285047167 2118 40-41 -2.5391166290353837 2118 42-43 0.4939741471050496 2118 44-45 -6.178619044236669 2118 46-47 -1.4992981501715263 2118 48-49 -1.9911872687216317 2118 50-51 -3.3374094509460406 2118 52-53 -7.601869621658864 2118 54-55 -2.8770854007543747 2118 56-57 -1.4710384857364005 2118 58-59 -3.1101642856069205 2118 60-61 -4.235096716466668 2118 62-63 -4.228468215127265 2118 64-65 -0.29796183473573024 2118 66-67 0.27002348647333463 2118 68-69 -2.438151579167396 2118 70-71 -0.7331228540439767 2118 72-73 0.04259390189016443 2118 74-75 -1.5348546122824622 2118 76 0.1763780285512695 2119 1 -0.3360483226172022 2119 2 -1.9849881920644599 2119 3 -0.333879584534877 2119 4 -0.43177060147971247 2119 5 0.1662416525156516 2119 6 0.15149164036748886 2119 7 -0.0968126233719957 2119 8 0.6230910109979533 2119 9 0.3354930225612023 2119 10-11 0.29948741002330337 2119 12-13 -1.235194135261466 2119 14-15 -0.028787109206867 2119 16-17 -2.070706815164378 2119 18-19 -0.5656671618531206 2119 20-21 -4.447177452515568 2119 22-23 -1.0139312960295541 2119 24-25 1.7275041499095494 2119 26-27 0.9683815145248929 2119 28-29 0.18523123259475938 2119 30-31 0.8687536247946497 2119 32-33 2.5512602528838784 2119 34-35 1.1898059485463932 2119 36-37 -3.20865214891095 2119 38-39 -3.496004601437047 2119 40-41 -4.760921140313581 2119 42-43 -1.3744469055265256 2119 44-45 -0.21621302919906782 2119 46-47 -1.3733583005474657 2119 48-49 -0.3107361409020797 2119 50-51 1.5667258873998193 2119 52-53 0.41128827307797877 2119 54-55 0.8917115917268248 2119 56-57 -2.4541211925033153 2119 58-59 -1.9973823307197023 2119 60-61 -0.3892320548125312 2119 62-63 -2.2961373880595985 2119 64-65 -2.2453302557883603 2119 66-67 0.9185197270748375 2119 68-69 -0.6261215039794266 2119 70-71 -0.44176947058533145 2119 72-73 -2.0006391808166057 2119 74-75 0.01777696666490769 2119 76 -2.8762535503961004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 3.0 24 21.0 25 41.0 26 106.0 27 133.0 28 287.0 29 402.0 30 662.0 31 921.0 32 1208.0 33 1631.0 34 2063.0 35 1484.0 36 309.0 37 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.1879280821917808 29.933647260273972 11.761558219178083 57.11686643835616 2 0.6207191780821918 3.2106164383561646 86.39768835616438 9.77097602739726 3 0.5779109589041096 34.417808219178085 62.1361301369863 2.868150684931507 4 0.3852739726027397 44.04965753424658 24.935787671232877 30.62928082191781 5 0.5136986301369862 52.57919520547946 42.74400684931507 4.163099315068494 6 0.545804794520548 46.11515410958904 9.235873287671232 44.103167808219176 7 1.0809075342465753 53.46746575342466 4.69820205479452 40.75342465753425 8 0.7170376712328768 44.9486301369863 29.83732876712329 24.497003424657535 9 0.6956335616438356 24.689640410958905 3.6601027397260273 70.95462328767124 10-11 0.8936215753424657 26.04345034246575 42.28381849315068 30.7791095890411 12-13 15.068493150684931 3.3497431506849313 33.09610445205479 48.48565924657534 14-15 23.828125 16.81292808219178 7.23458904109589 52.124357876712324 16-17 32.38976883561644 26.856806506849317 0.898972602739726 39.85445205479452 18-19 25.77589897260274 21.982020547945204 0.8026541095890412 51.439426369863014 20-21 17.299871575342465 10.33818493150685 0.898972602739726 71.46297089041096 22-23 23.98330479452055 39.800941780821915 0.8347602739726028 35.38099315068493 24-25 32.73758561643836 43.95869006849315 1.209332191780822 22.09439212328767 26-27 41.80757705479452 32.78039383561644 14.84375 10.56827910958904 28-29 44.46703767123288 8.149614726027398 23.46960616438356 23.913741438356166 30-31 62.933433219178085 1.6053082191780823 31.80115582191781 3.6601027397260273 32-33 55.902183219178085 1.5303938356164384 41.30458047945205 1.2628424657534247 34-35 39.025042808219176 17.289169520547944 41.89854452054795 1.7872431506849313 36-37 26.193279109589042 27.38120719178082 29.7142551369863 16.711258561643834 38-39 17.449700342465754 10.209760273972602 30.42594178082192 41.914597602739725 40-41 9.717465753424657 16.39019691780822 41.17080479452055 32.72153253424658 42-43 37.85851883561644 9.599743150684931 44.54195205479452 7.99978595890411 44-45 11.488655821917808 23.78531678082192 33.62585616438356 31.10017123287671 46-47 39.37821061643836 3.0126284246575343 22.23351883561644 35.37564212328767 48-49 28.34439212328767 1.4233732876712328 24.20269691780822 46.02953767123288 50-51 7.314854452054795 1.2307363013698631 48.52846746575342 42.925941780821915 52-53 15.03638698630137 1.5036386986301369 67.08583047945206 16.37414383561644 54-55 9.305436643835616 1.6106592465753424 49.32577054794521 39.75813356164384 56-57 37.69798801369863 1.8193493150684932 31.88142123287671 28.601241438356162 58-59 11.247859589041095 31.308861301369863 49.30971746575342 8.133561643835616 60-61 23.40004280821918 65.00963184931507 9.58369006849315 2.0066352739726026 62-63 2.573844178082192 94.63291952054794 1.0702054794520548 1.723030821917808 64-65 0.5136986301369862 96.57534246575342 0.7705479452054794 2.1404109589041096 66-67 0.4227311643835617 94.17273116438356 1.0059931506849316 4.398544520547945 68-69 1.1130136986301369 84.6586044520548 2.905607876712329 11.32277397260274 70-71 2.34375 74.84482020547945 5.714897260273973 17.096532534246574 72-73 3.8794948630136985 61.451198630136986 9.17166095890411 25.497645547945208 74-75 4.692851027397261 58.7275256849315 9.36964897260274 27.209974315068493 76 3.6708047945205475 62.007705479452056 8.37970890410959 25.94178082191781 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.0 23 1.0 24 1.0 25 0.0 26 0.0 27 0.5 28 1.0 29 1.0 30 2.0 31 2.5 32 2.0 33 2.0 34 3.0 35 4.5 36 6.5 37 8.0 38 14.0 39 39.5 40 188.0 41 855.0 42 1393.0 43 1827.5 44 2201.5 45 1836.5 46 1532.0 47 1181.0 48 621.5 49 290.5 50 168.0 51 124.0 52 54.5 53 28.0 54 27.0 55 22.0 56 14.0 57 10.0 58 9.0 59 5.0 60 2.0 61 1.5 62 0.0 63 0.0 64 0.0 65 1.0 66 1.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 9344.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.428938356164384 #Duplication Level Percentage of deduplicated Percentage of total 1 79.67479674796748 14.68321917808219 2 8.652729384436702 3.1892123287671237 3 3.077816492450639 1.701626712328767 4 1.3937282229965158 1.0273972602739725 5 1.3937282229965158 1.2842465753424657 6 0.6387921022067364 0.7063356164383561 7 0.7549361207897793 0.9738869863013699 8 0.6387921022067364 0.9417808219178082 9 0.23228803716608595 0.3852739726027397 >10 2.5551684088269457 8.465325342465754 >50 0.5807200929152149 7.04195205479452 >100 0.23228803716608595 9.118150684931507 >500 0.05807200929152149 6.560359589041095 >1k 0.11614401858304298 43.92123287671233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 2224 23.8013698630137 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT 1880 20.11986301369863 TruSeq Adapter, Index 5 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 613 6.560359589041095 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 301 3.221318493150685 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 262 2.803938356164384 No Hit TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT 160 1.7123287671232876 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT 129 1.3805650684931507 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 77 0.8240582191780822 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 76 0.8133561643835616 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT 76 0.8133561643835616 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT 73 0.78125 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 65 0.6956335616438356 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT 65 0.6956335616438356 No Hit CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 65 0.6956335616438356 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT 58 0.6207191780821918 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 52 0.5565068493150684 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTCTT 51 0.545804794520548 No Hit CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 48 0.5136986301369862 No Hit TTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 34 0.3638698630136986 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCG 32 0.3424657534246575 No Hit CTTATACACATCTCCGAGCCCACGCGACGTGTGGTGATCTCGTATGCCGT 32 0.3424657534246575 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTGTGGTGATCTCGTATGCCGT 32 0.3424657534246575 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCG 29 0.3103595890410959 No Hit CTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT 28 0.2996575342465753 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGCTCTCGTATGCCGTCTT 27 0.2889554794520548 No Hit ATACACATCTCCGAGCCCACGAGAAGTGTGGTGATCTCGTATGCCGTCTT 23 0.2461472602739726 Illumina PCR Primer Index 5 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTC 23 0.2461472602739726 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCG 22 0.23544520547945205 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGT 21 0.2247431506849315 No Hit CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTC 21 0.2247431506849315 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 21 0.2247431506849315 No Hit CTTATACACATCTCCGAGCACACGAGACGTGTGGTGATCTCGTATGCCGT 20 0.21404109589041095 No Hit CTTATCACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 19 0.2033390410958904 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGTGGTGATCTCGTATGCCGT 18 0.19263698630136986 No Hit ATACACATCTCCGAGCCCACGAGACGCGTGGTGATCTCGTATGCCGTCTT 16 0.17123287671232876 Illumina PCR Primer Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGAAGTGTGGTGATCTCGTATGCCGT 16 0.17123287671232876 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCAGT 15 0.1605308219178082 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCAGTCTT 15 0.1605308219178082 No Hit TCTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 15 0.1605308219178082 No Hit ATACACATCTCCGAGCCCACGAGACGAGTGGTGATCTCGTATGCCGTCTT 15 0.1605308219178082 TruSeq Adapter, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACGTGAGGTGATCTCGTATGCCGTCTT 14 0.14982876712328766 TruSeq Adapter, Index 5 (95% over 21bp) CCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 14 0.14982876712328766 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTAGTATGCCGT 14 0.14982876712328766 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTCTGCCGTCTT 14 0.14982876712328766 No Hit ACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCT 14 0.14982876712328766 RNA PCR Primer, Index 5 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCACGTATGCCGT 14 0.14982876712328766 No Hit TCTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 13 0.1391267123287671 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAAATCGTATGCCGT 13 0.1391267123287671 No Hit CTTATTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 12 0.12842465753424656 No Hit ATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTGCTT 12 0.12842465753424656 TruSeq Adapter, Index 5 (96% over 27bp) CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGTC 12 0.12842465753424656 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGAGCTCGTATGCCGTCTT 11 0.11772260273972603 No Hit CTTACACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 11 0.11772260273972603 No Hit CTTATACACATATCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 11 0.11772260273972603 No Hit ATTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 10 0.10702054794520548 No Hit ATACACATCTACGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT 10 0.10702054794520548 TruSeq Adapter, Index 5 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACATCGTATGCCGT 10 0.10702054794520548 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGCTCTCGTATGCCGT 10 0.10702054794520548 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGAGATCTCGTATGCCGTCTT 10 0.10702054794520548 RNA PCR Primer, Index 17 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCCT 10 0.10702054794520548 No Hit CTTATACACATCTCCGAGCCCACGTGACGTGTGGTGATCTCGTATGCCGT 10 0.10702054794520548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.010702054794520547 0.0 6 0.0 0.0 0.0 0.010702054794520547 0.0 7 0.0 0.0 0.0 0.010702054794520547 0.0 8 0.0 0.0 0.0 0.010702054794520547 0.0 9 0.0 0.0 0.0 0.010702054794520547 0.0 10 0.0 0.0 0.0 0.010702054794520547 0.0 11 0.0 0.0 0.0 0.010702054794520547 0.0 12 0.0 0.0 0.0 0.010702054794520547 0.0 13 0.0 0.0 0.0 0.010702054794520547 0.0 14 0.0 0.0 0.0 0.010702054794520547 0.0 15 0.0 0.0 0.0 0.010702054794520547 0.0 16 0.0 0.0 0.0 0.010702054794520547 0.0 17 0.0 0.0 0.0 0.010702054794520547 0.0 18 0.0 0.0 0.0 0.010702054794520547 0.0 19 0.0 0.0 0.0 0.010702054794520547 0.0 20 0.0 0.0 0.0 0.021404109589041095 0.0 21 0.0 0.0 0.0 0.021404109589041095 0.0 22 0.0 0.0 0.0 0.021404109589041095 0.0 23 0.0 0.0 0.0 0.03210616438356165 0.0 24 0.0 0.0 0.0 0.03210616438356165 0.0 25 0.0 0.0 0.0 0.04280821917808219 0.0 26 0.0 0.0 0.0 0.04280821917808219 0.0 27 0.0 0.0 0.0 0.04280821917808219 0.0 28 0.0 0.0 0.0 0.04280821917808219 0.0 29 0.0 0.0 0.0 0.04280821917808219 0.0 30 0.0 0.0 0.0 0.04280821917808219 0.0 31 0.0 0.0 0.0 0.04280821917808219 0.0 32 0.0 0.0 0.0 0.04280821917808219 0.0 33 0.0 0.0 0.0 0.04280821917808219 0.0 34 0.0 0.0 0.0 0.05351027397260274 0.0 35 0.0 0.0 0.0 0.07491438356164383 0.0 36 0.0 0.0 0.0 0.07491438356164383 0.0 37 0.0 0.0 0.0 0.09631849315068493 0.0 38 0.0 0.0 0.0 0.11772260273972603 0.0 39 0.0 0.0 0.0 0.17123287671232876 0.0 40 0.0 0.0 0.0 0.1819349315068493 0.0 41 0.0 0.0 0.0 0.21404109589041095 0.0 42 0.0 0.0 0.0 0.2568493150684932 0.0 43 0.0 0.0 0.0 0.2889554794520548 0.0 44 0.0 0.0 0.0 0.3424657534246575 0.0 45 0.0 0.0 0.0 0.3852739726027397 0.0 46 0.0 0.0 0.0 0.4173801369863014 0.0 47 0.0 0.0 0.0 0.4387842465753425 0.0 48 0.0 0.0 0.0 0.4815924657534247 0.0 49 0.0 0.0 0.0 0.5136986301369864 0.0 50 0.0 0.0 0.0 0.5351027397260274 0.0 51 0.0 0.0 0.0 0.5779109589041096 0.0 52 0.0 0.0 0.0 0.5993150684931506 0.0 53 0.0 0.0 0.0 0.6314212328767124 0.0 54 0.0 0.0 0.0 0.663527397260274 0.0 55 0.0 0.0 0.0 0.684931506849315 0.0 56 0.0 0.0 0.0 0.6956335616438356 0.0 57 0.0 0.0 0.0 0.7170376712328768 0.0 58 0.0 0.0 0.0 0.7491438356164384 0.0 59 0.0 0.0 0.0 0.7705479452054794 0.0 60 0.0 0.0 0.0 0.78125 0.0 61 0.0 0.0 0.0 0.8454623287671232 0.0 62 0.0 0.0 0.0 0.8668664383561644 0.0 63 0.0 0.0 0.0 0.8882705479452054 0.0 64 0.0 0.0 0.0 0.9203767123287672 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 15 0.002155968 70.0 1 CTTTACA 115 0.0 70.0 1 ACACACA 15 0.002155968 70.0 70 ACGAGTG 15 0.002155968 70.0 27 CTTATCA 15 0.002155968 70.0 1 GACGAGT 15 0.002155968 70.0 26 TCTTTAT 15 0.002155968 70.0 1 TTTACAC 115 0.0 70.0 2 TTACACA 120 0.0 67.08333 3 TCTTATA 100 0.0 55.999996 1 CTTATAC 440 0.0 54.886368 1 CACAAAA 20 0.0067382553 52.5 69 TTATACA 470 0.0 51.38298 2 TATACAC 505 0.0 47.82178 3 GGTGATA 50 3.122319E-6 42.0 33 GATATCG 50 3.122319E-6 42.0 36 TATCGTA 50 3.122319E-6 42.0 38 TGATATC 50 3.122319E-6 42.0 35 GTGATAT 50 3.122319E-6 42.0 34 ATATCGT 50 3.122319E-6 42.0 37 >>END_MODULE