FastQCFastQC Report
Wed 25 May 2016
SRR1780363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99128
Sequences flagged as poor quality0
Sequence length50
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1360.13719635219110643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAT254.4334356E-543.9661985
TATACAG254.4334356E-543.9661983
CTTTACA850.038.832891
TTTACAC1050.035.5916822
TCTTAAT250.002339639235.2084851
CTGATAC250.002339639235.2084851
ATTTCGG250.002345447735.19071242
TGATACA250.002351267335.172962
GTCTGAC250.002351267335.172967
TTATCAC250.002351267335.172962
ATACAGA250.002351267335.172964
CTTATGC401.8003613E-533.0079541
CTATACA900.031.7854391
CCTATAC353.1922176E-431.4361461
GCTCAGA353.1922176E-431.4361461
TTACACA1150.030.585183
GTTATAC454.025743E-529.3404051
TATCACA300.00574145229.3107973
TTATACA5350.028.7629322
GATACAC407.017826E-427.4788723
Produced by FastQC (version 0.11.5)