##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780363_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99128 Sequences flagged as poor quality 0 Sequence length 50 %GC 58 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.5551912678557 16.0 10.0 27.0 7.0 30.0 2 17.996237188281818 16.0 10.0 27.0 7.0 31.0 3 18.30024816398999 16.0 10.0 27.0 7.0 31.0 4 19.944980227584537 18.0 10.0 30.0 7.0 35.0 5 20.08805786457913 18.0 10.0 30.0 7.0 35.0 6 20.37156000322815 17.0 10.0 30.0 7.0 35.0 7 19.938897183439593 17.0 10.0 30.0 7.0 35.0 8 19.89741546283593 17.0 8.0 31.0 7.0 35.0 9 20.12978169639254 17.0 8.0 31.0 7.0 37.0 10 19.968868533613108 17.0 8.0 31.0 7.0 37.0 11 20.426660479380196 17.0 10.0 31.0 7.0 37.0 12 20.16411104834154 17.0 8.0 31.0 7.0 37.0 13 20.27040795738843 17.0 8.0 31.0 7.0 37.0 14 20.511036235977727 17.0 8.0 31.0 7.0 37.0 15 20.131214187716893 17.0 8.0 31.0 7.0 37.0 16 20.022122911790817 17.0 8.0 31.0 7.0 37.0 17 20.190349850698087 17.0 8.0 31.0 7.0 37.0 18 19.829543620369623 17.0 8.0 30.0 7.0 37.0 19 19.80837906545073 18.0 8.0 30.0 7.0 37.0 20 19.94187313372609 18.0 8.0 30.0 7.0 37.0 21 19.70071019288193 17.0 8.0 30.0 7.0 37.0 22 19.706218222903722 17.0 8.0 30.0 7.0 37.0 23 19.637650310709386 17.0 8.0 30.0 7.0 37.0 24 19.07697118876604 16.0 8.0 30.0 7.0 37.0 25 19.057864579130015 16.0 8.0 30.0 7.0 36.0 26 18.751321523686546 16.0 8.0 29.0 7.0 36.0 27 18.511903801145994 15.0 8.0 28.0 7.0 36.0 28 18.43935114195787 15.0 8.0 28.0 7.0 36.0 29 18.148555403115164 15.0 8.0 28.0 7.0 35.0 30 18.029345896214995 15.0 8.0 27.0 7.0 35.0 31 17.926035025421676 15.0 8.0 27.0 6.0 35.0 32 17.60679121943346 15.0 8.0 27.0 6.0 35.0 33 17.67193931079009 15.0 8.0 27.0 6.0 35.0 34 17.267442095069 13.0 8.0 26.0 6.0 35.0 35 17.19496005165039 13.0 8.0 26.0 6.0 34.0 36 17.100647647486078 13.0 8.0 26.0 6.0 34.0 37 16.887297231861837 13.0 8.0 25.0 6.0 34.0 38 16.994653377451375 13.0 8.0 25.0 6.0 34.0 39 16.630780405132757 13.0 8.0 25.0 6.0 33.0 40 16.14029335808248 12.0 7.0 24.0 6.0 33.0 41 16.245288919376968 13.0 7.0 24.0 6.0 33.0 42 16.299985876846097 13.0 8.0 24.0 7.0 33.0 43 16.346007182632555 13.0 8.0 24.0 7.0 33.0 44 16.356438140585908 13.0 8.0 24.0 7.0 33.0 45 16.417470341376806 13.0 7.0 24.0 7.0 33.0 46 16.27084173997256 12.0 7.0 24.0 7.0 33.0 47 16.229117908159147 12.0 7.0 24.0 7.0 33.0 48 16.200266322330723 12.0 7.0 24.0 7.0 33.0 49 15.901702848841902 12.0 7.0 24.0 7.0 32.0 50 15.886066499878945 12.0 7.0 24.0 7.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5533940783985898 1101 2 -0.6363929087804294 1101 3 -0.3250691372207548 1101 4 -0.3209974313298538 1101 5 -0.13955229986779472 1101 6 -0.6431393029886117 1101 7 -0.6758424505513183 1101 8 -1.4795479466396344 1101 9 -1.2303839470500968 1101 10 -2.2103506012111467 1101 11 -0.9880530122275175 1101 12 -0.45165376626303555 1101 13 -0.24418767554622534 1101 14 -0.49197007703100937 1101 15 -0.11562718738487021 1101 16 -0.3223761248296064 1101 17 0.22413885197385497 1101 18 -0.6215583798131412 1101 19 -1.336497851765479 1101 20 -0.9218733119853226 1101 21 -1.3115580744275057 1101 22 -0.18715668458949608 1101 23 -1.2166297341775163 1101 24 -0.01766829347223009 1101 25 0.461141774864565 1101 26 -0.8475200601674366 1101 27 -0.8231156952292906 1101 28 -0.8470911666793199 1101 29 -0.5403175186735254 1101 30 0.2547440959804561 1101 31 0.16255563301407605 1101 32 -0.7543278608947084 1101 33 -0.6511572380834281 1101 34 -0.623141708518709 1101 35 -0.6403576953690084 1101 36 -1.1968927606780415 1101 37 -0.3512091278890743 1101 38 -1.5433338618493178 1101 39 -0.15094229877157161 1101 40 -0.1830571494007387 1101 41 -0.2617986206941829 1101 42 -0.5272471472194535 1101 43 0.27231376806953733 1101 44 -0.30818624965410635 1101 45 0.19606204259498128 1101 46 0.35713650027741295 1101 47 0.32568555316453285 1101 48 -0.3844245725438764 1101 49 -0.03643148800073526 1101 50 -0.008905715753254384 1102 1 0.10445067277274234 1102 2 -0.7673709173068737 1102 3 -5.577193827548399E-4 1102 4 -0.4080347028675675 1102 5 -0.8053747640947186 1102 6 -0.7583307258918239 1102 7 -0.587918766451871 1102 8 -0.9472360982583901 1102 9 -0.6651482035187968 1102 10 -1.2504924272210225 1102 11 0.4960126667222937 1102 12 -0.6268679826321204 1102 13 0.3825904258424906 1102 14 0.44002941644752624 1102 15 -0.10853588689101201 1102 16 0.33015015097133116 1102 17 -0.03473582617396431 1102 18 -1.1629454044216416 1102 19 -0.6877282768214101 1102 20 -0.28336079668415337 1102 21 -0.8199367836419746 1102 22 -0.2620374409948809 1102 23 0.1387372828331621 1102 24 -0.04535813349586704 1102 25 -0.24976109964474347 1102 26 -1.1963445082700908 1102 27 -0.9198190847020875 1102 28 -1.2930833155966326 1102 29 -0.7208924205349909 1102 30 -0.8100062311479874 1102 31 -0.15992969896853282 1102 32 0.48348396638147406 1102 33 0.2616366498909066 1102 34 -0.2498353896634491 1102 35 -0.7039261390818119 1102 36 -1.3591484695970415 1102 37 -1.367839915952052 1102 38 -1.1045596437918288 1102 39 -0.09919268921520086 1102 40 -0.8866576728062512 1102 41 -0.587154240971925 1102 42 -0.20851851609370797 1102 43 -0.2910647300943232 1102 44 0.30183443330566817 1102 45 -0.5526611012149036 1102 46 0.30361406559757853 1102 47 0.0969066919936239 1102 48 -0.467409672370815 1102 49 -0.7529483087343465 1102 50 -0.15187139892407764 1103 1 -0.08385861320579835 1103 2 0.29478224584517676 1103 3 0.0072114260112492445 1103 4 0.29118838259303104 1103 5 0.2921711725189198 1103 6 1.0893693604320127 1103 7 0.4600526233619142 1103 8 -0.04650851162625358 1103 9 0.6215702574504967 1103 10 1.34551201229063 1103 11 1.0576108909176334 1103 12 1.1330284287626249 1103 13 1.3928259665996734 1103 14 0.2614623921535326 1103 15 1.025882660402118 1103 16 1.2599812028889517 1103 17 0.12829290733201404 1103 18 0.039668974663324974 1103 19 0.8743632264465653 1103 20 0.22459332836209 1103 21 1.466658357559158 1103 22 1.1372719683034305 1103 23 1.222594725181171 1103 24 -0.012185115861139906 1103 25 -0.2905799429053175 1103 26 -0.1663778046074924 1103 27 0.6111958369898609 1103 28 0.24594739102564134 1103 29 1.4748160515330824 1103 30 1.8578936332002662 1103 31 0.7997447368435289 1103 32 -0.06331588563480395 1103 33 0.4668407441072091 1103 34 0.3268078462472701 1103 35 1.0168779058065844 1103 36 0.555267611300728 1103 37 0.7425312765501069 1103 38 0.3449409111471269 1103 39 0.2065170160505545 1103 40 -0.1330505695876063 1103 41 -0.5094627870318593 1103 42 -0.20259916722208615 1103 43 0.634203490401422 1103 44 0.4473517597330954 1103 45 0.9358085004622438 1103 46 0.059070965338605674 1103 47 -0.5275846139627198 1103 48 0.1993803140640118 1103 49 -0.11859142132529499 1103 50 0.39702488915089873 1104 1 0.5281323370204447 1104 2 0.6156721402644827 1104 3 0.4679957397367396 1104 4 0.13357148968202281 1104 5 1.35463974969338 1104 6 0.2727529702862128 1104 7 0.3426569471427108 1104 8 1.5072873656990353 1104 9 1.549172067405248 1104 10 1.506522570360012 1104 11 1.6047451292283412 1104 12 1.3675683985967133 1104 13 1.1472251621753387 1104 14 0.9870782795341242 1104 15 0.9702014135444017 1104 16 1.0617660193796503 1104 17 0.46187762326967174 1104 18 0.34987510839886937 1104 19 0.7032973642042322 1104 20 -0.2554217545941668 1104 21 1.1347076285496058 1104 22 0.7222392885648681 1104 23 0.4298596824461285 1104 24 1.410011968100651 1104 25 0.7718886342691427 1104 26 0.9561712150003494 1104 27 1.0008388736917198 1104 28 1.0577875116892912 1104 29 1.5257210289086451 1104 30 1.177777997202277 1104 31 0.4004234698752036 1104 32 2.112777628694001 1104 33 1.03534250378544 1104 34 0.7508902997414886 1104 35 0.6142886649820518 1104 36 1.1052927495611584 1104 37 0.8817972393454809 1104 38 0.3883044103929798 1104 39 0.3744405957388395 1104 40 1.0053355540926354 1104 41 0.8291495809922758 1104 42 0.21746116460295184 1104 43 0.8389546216231416 1104 44 0.6808861991498887 1104 45 1.279825594479341 1104 46 0.4176682504064786 1104 47 0.5214349938804155 1104 48 0.09354823764369868 1104 49 0.8978741392579117 1104 50 -0.06539341150269529 1105 1 -0.7961941803440489 1105 2 -0.5632470418192561 1105 3 -0.2830591125839348 1105 4 0.6900466600812436 1105 5 -1.4362560633543318 1105 6 -0.9949360917066485 1105 7 -1.8132989113447948 1105 8 0.1538306957866702 1105 9 0.3442061472494551 1105 10 -0.4865803183124946 1105 11 0.2427684982643683 1105 12 -0.5970534774175675 1105 13 0.0456347694457051 1105 14 -0.9010262319881086 1105 15 -0.34844512978568787 1105 16 0.44646254648124284 1105 17 -0.9577913153866078 1105 18 0.3855778023294114 1105 19 -0.6792894582560081 1105 20 0.4747794787716231 1105 21 -1.3011086648616228 1105 22 -0.5878872937136137 1105 23 -1.520724543454552 1105 24 -0.5832532055442705 1105 25 -0.7574038032511439 1105 26 -1.7242085318350924 1105 27 -0.7708261701252219 1105 28 -2.2282397521860737 1105 29 -2.021585040549322 1105 30 -0.8646595230833434 1105 31 -1.0978725379144763 1105 32 -1.5604615793553336 1105 33 -1.0048230268864202 1105 34 -1.9078244032770133 1105 35 -1.7004630390101596 1105 36 0.13336804978835914 1105 37 -1.5979171391030746 1105 38 -0.6554313896719322 1105 39 -0.7281235068252627 1105 40 -1.8082801550926941 1105 41 -1.1716618438697832 1105 42 -0.9100948237125301 1105 43 -1.2778094160269724 1105 44 -0.4848067576823105 1105 45 -0.3757245515771359 1105 46 -1.5470347680940062 1105 47 -0.24860720021241178 1105 48 -1.2722420901719467 1105 49 -1.2834793174120005 1105 50 -1.083325900954172 1106 1 0.2506488424710831 1106 2 0.7175837017568831 1106 3 0.05981210700220885 1106 4 -0.6533930427914925 1106 5 -0.3357103193992046 1106 6 0.0863949171616909 1106 7 -0.43893371768148626 1106 8 0.07223439471792048 1106 9 -0.7060098763994525 1106 10 -0.35579126351070656 1106 11 -0.13212727979975725 1106 12 -0.15193106401651946 1106 13 -0.0011703544836514368 1106 14 0.38670210879867284 1106 15 -0.23281562929300037 1106 16 -0.07116043540309036 1106 17 0.17330093050136242 1106 18 0.571585533702109 1106 19 0.5238485694859776 1106 20 -0.15934467797816865 1106 21 0.6662982933738029 1106 22 -0.2627906515548908 1106 23 -0.21120434334843807 1106 24 -0.1597331570336955 1106 25 0.2032950522025061 1106 26 0.4097640293716083 1106 27 0.5512764600574513 1106 28 -0.05932441829689239 1106 29 0.34542776927407104 1106 30 -0.5520316143438606 1106 31 -0.02484138503037059 1106 32 0.17538318680854204 1106 33 0.27843324537917624 1106 34 0.5687161864296506 1106 35 0.2878262052860592 1106 36 0.290307335773349 1106 37 -0.11743506527604453 1106 38 -0.04885972287893736 1106 39 -0.674231291256401 1106 40 -0.4858743338238387 1106 41 -0.46249789328992996 1106 42 0.02975964456610214 1106 43 -0.8045660293825403 1106 44 -0.5405939175398107 1106 45 -0.16731857871273803 1106 46 0.2809800882692173 1106 47 0.5264250138404982 1106 48 -0.33382777955267784 1106 49 0.3823283078438173 1106 50 0.3532692631480039 1107 1 -1.2095161933110141 1107 2 -0.6165042199606567 1107 3 -0.9446058862795184 1107 4 -1.2646461463404925 1107 5 -1.2785685054660938 1107 6 0.21678048748445988 1107 7 -0.24614855629694077 1107 8 -0.4096919964022767 1107 9 0.03979120436584793 1107 10 -0.680312951841362 1107 11 -0.6743355461937988 1107 12 -0.5143890748241731 1107 13 -1.3967085463925173 1107 14 -0.2646415453564295 1107 15 -1.410192582703253 1107 16 -1.3947693152044636 1107 17 -1.313919750088676 1107 18 -0.6771730466980657 1107 19 -0.19789087844742426 1107 20 -0.4231828552821 1107 21 -0.2459990631326967 1107 22 0.15516611783316137 1107 23 -0.9040990417564991 1107 24 -0.8384245547261173 1107 25 -0.4141651493456173 1107 26 -1.2145604923167248 1107 27 -0.7106995878467401 1107 28 -0.6542199930011421 1107 29 -0.15658040949035268 1107 30 -0.5528348858496308 1107 31 -0.5137416333142895 1107 32 0.10980702206486015 1107 33 -0.7170027119744091 1107 34 -0.6168395314029773 1107 35 -1.0193886333293953 1107 36 -1.4633438983437816 1107 37 -1.8571020727245635 1107 38 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2 0.6202972619782123 2214 3 0.20428265956796565 2214 4 -0.29096599814660706 2214 5 -0.05018011398724198 2214 6 0.25189935133132835 2214 7 0.7879669114400158 2214 8 0.5145901894575537 2214 9 -0.7697179033834587 2214 10 -1.113162596793508 2214 11 -0.20026947641917658 2214 12 -1.0780661356454146 2214 13 -0.030314597642902896 2214 14 0.3647965528220176 2214 15 -0.10793880067176076 2214 16 -0.09151213706306294 2214 17 -0.6413356191840762 2214 18 -0.12708042551868814 2214 19 -0.6792894582560081 2214 20 -0.885980014899264 2214 21 -1.3074377787856726 2214 22 -0.6764948886503213 2214 23 -0.008066315606452434 2214 24 0.2648480602785135 2214 25 0.8691784752298695 2214 26 0.4909813415826285 2214 27 -0.04297806885939792 2214 28 -0.22191063826202395 2214 29 -1.021585040549322 2214 30 0.07204933767614818 2214 31 -0.11685987968662914 2214 32 -0.7819805666971043 2214 33 0.033151656657882 2214 34 0.06685914102678225 2214 35 0.3754863280784484 2214 36 -0.23372055780657774 2214 37 -0.6738665061916826 2214 38 -0.5351782251149722 2214 39 -0.2534399625214654 2214 40 -0.3335966107888968 2214 41 0.208084991573255 2214 42 0.40003175856595163 2214 43 0.2981399510616356 2214 44 -0.1430346057835763 2214 45 0.08630076487856186 2214 46 -0.10399679341046308 2214 47 0.02354469852176777 2214 48 0.10750474527109333 2214 49 -0.2834793174120005 2214 50 -0.4947183060174627 2215 1 0.05339628875498548 2215 2 0.5888998929040028 2215 3 0.28172122993282045 2215 4 -0.4408377611978196 2215 5 0.5340012378783996 2215 6 0.6449249159875698 2215 7 -0.3504048929779522 2215 8 0.26068103085740546 2215 9 0.026799015620429856 2215 10 0.5546623390880185 2215 11 0.730813505296716 2215 12 0.3487147032724316 2215 13 -0.33920017719117723 2215 14 -0.9607598300686888 2215 15 -0.7126794311011508 2215 16 -0.896881542209087 2215 17 0.16097264589410543 2215 18 -0.37209573121903006 2215 19 0.45606257285179197 2215 20 -0.553184449415685 2215 21 0.5058740438336677 2215 22 0.10459222974133908 2215 23 0.3598496271419549 2215 24 -0.14944001782192373 2215 25 0.0492893381404329 2215 26 0.7225110842813969 2215 27 0.5523723075780964 2215 28 -0.6690852887129211 2215 29 -0.6101512687283552 2215 30 0.1324034371218339 2215 31 -0.4420853285159474 2215 32 -0.15481915360866338 2215 33 -1.3305448768078243 2215 34 -0.627440519765802 2215 35 -0.19227242099080044 2215 36 0.16964669626804607 2215 37 0.4549495772036991 2215 38 -0.5308760823169223 2215 39 -0.5255091277402961 2215 40 -0.06115514475100525 2215 41 -0.47678304846976616 2215 42 -0.34639001689528826 2215 43 -0.3396527187489049 2215 44 -0.7683345147767096 2215 45 -0.8223614341782781 2215 46 -0.07164798890975987 2215 47 0.1586898958411993 2215 48 0.251667895763358 2215 49 0.45003602965509515 2215 50 0.5212078956868496 2216 1 -0.06289330400519688 2216 2 0.7810567556491002 2216 3 0.7808162525572548 2216 4 0.8335714896820221 2216 5 0.4431012881549208 2216 6 0.40224014977339095 2216 7 -0.6547789502931884 2216 8 0.26113351954519004 2216 9 -0.12005870182552059 2216 10 1.2360097498471916 2216 11 0.4547451292283391 2216 12 1.162440193468509 2216 13 0.9138918288420044 2216 14 0.9575911000469475 2216 15 1.8663552596982491 2216 16 0.5758685834822153 2216 17 0.6003391617312097 2216 18 1.1550033135270752 2216 19 0.14816915907602635 2216 20 0.5766295274571149 2216 21 2.182143525985502 2216 22 -0.24699148066590126 2216 23 -0.24321724063079486 2216 24 0.2112940193827022 2216 25 -0.7729831606026529 2216 26 -1.393828784999652 2216 27 -0.3837765109236635 2216 28 -1.7614432575414796 2216 29 -1.3114584582708417 2216 30 -0.8440168745925938 2216 31 -0.8649611455094117 2216 32 -0.19619673028035578 2216 33 0.5776501960931313 2216 34 0.6816595305107178 2216 35 1.5181348188282051 2216 36 -0.4459893017208927 2216 37 0.29205364960188973 2216 38 0.8947146668032353 2216 39 0.571876493174738 2216 40 0.8912329899900726 2216 41 0.38812393996663275 2216 42 0.27130731844910727 2216 43 -0.008481275812755484 2216 44 0.5988349170986069 2216 45 -0.23940517475142897 2216 46 0.5138220965603253 2216 47 0.0881016605470819 2216 48 0.2114969555924162 2216 49 0.27095106233483435 2216 50 0.5640937679844846 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1.0 3 0.0 4 2.0 5 4.0 6 165.0 7 2423.0 8 5775.0 9 7199.0 10 6342.0 11 5298.0 12 4970.0 13 5088.0 14 5178.0 15 4993.0 16 4628.0 17 4274.0 18 3947.0 19 3478.0 20 3165.0 21 3019.0 22 2637.0 23 2434.0 24 2410.0 25 2249.0 26 2073.0 27 1965.0 28 1962.0 29 1705.0 30 1566.0 31 1451.0 32 1395.0 33 1293.0 34 1143.0 35 1058.0 36 916.0 37 804.0 38 719.0 39 1399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.680602682178055 17.6129019227662 8.971562449507191 26.734932945548557 2 25.292818013982625 24.163917556974667 31.182470264217184 19.36079416482552 3 35.928294730046 19.379993543701072 25.278427891211365 19.41328383504156 4 37.04742401162145 17.48161449453731 20.928707617500784 24.542253876340453 5 31.12976282950155 26.15937131154983 27.632229362333167 15.078636496615452 6 44.55855056089097 23.240658542490518 12.223589702203212 19.9772011944153 7 36.59850494819777 23.3538793668728 18.29673045688864 21.750885228040794 8 30.46899432041724 31.08638413348533 18.070757714850647 20.373863831246787 9 35.61996227062254 17.985009129702302 13.506915371190493 32.88811322848467 10 28.791348472161975 23.612133929201935 27.665519989508407 19.930997609127683 11 36.66142081794887 19.51758368137522 20.16827068579384 23.65272481488207 12 28.758347961180718 21.45552125577548 24.42346104957327 25.362669733470533 13 32.41659015930347 16.696092575590956 22.28129823747213 28.60601902763345 14 40.16122197784459 17.754595532597513 17.399463266006173 24.684719223551728 15 30.87058728573302 22.78620216511799 19.486061926813765 26.85714862233522 16 37.26827297065212 17.944132949436785 19.18573521240592 25.60185886750518 17 43.80939889828285 17.408543352367882 15.993058778425715 22.788998970923547 18 37.900862852870446 15.928425647139651 17.23280861640431 28.937902883585586 19 34.238185713708205 17.071593160043662 20.595666410639932 28.0945547156082 20 38.41961026221849 16.947627089506202 21.13523516741331 23.497527480861994 21 31.227243304474474 19.5646896998139 17.400275103163686 31.807791892547943 22 38.126047197044635 15.797282839089974 15.616609806811072 30.460060157054325 23 45.07269626983887 17.52176852216202 15.844860812624228 21.560674395374882 24 33.15692730320965 26.760862100309048 15.439230815844224 24.64297978063708 25 41.362604087812265 18.821095129952056 14.880645975271259 24.935654806964422 26 37.468717203519816 25.10393961411157 15.20444821183499 22.222894970533623 27 33.81367562767417 24.60946960523129 19.203600548962623 22.373254218131912 28 41.203241203241205 21.05369378096651 16.94265330628967 20.80041170950262 29 35.451251879585435 20.70521036219233 20.81621943466107 23.027318323561172 30 41.17712382009994 18.92282065519156 18.0213012972591 21.878754227449395 31 36.8256915918977 18.555150732214408 21.741368347748857 22.877789328139034 32 33.82514708998981 22.021616930234437 20.511863072591307 23.64137290718445 33 37.609373580792635 19.464712828120742 22.306659804010618 20.619253787076 34 32.77621340115663 21.10394525691101 22.336270324280136 23.783571017652225 35 35.84995155821088 21.683755853382852 20.77143549168416 21.694857096722107 36 39.587622623228775 17.85656614589641 21.825925477372735 20.729885753502078 37 34.25941811062559 18.739340606109536 24.710619531541713 22.290621751723165 38 35.868117229129666 16.87590828354594 24.312732116906187 22.943242370418215 39 34.338541035888746 18.014008316176174 26.59965281982964 21.04779782810545 40 29.71615654335459 28.810767660640845 22.36694522195164 19.10613057405293 41 32.56766848999859 15.344246412207577 26.895827866701655 25.19225723109218 42 26.644799919261242 18.178331735378716 32.85663823989504 22.320230105465004 43 27.028145277668454 16.23929036359783 29.571210882706144 27.16135347602757 44 27.341069626639758 15.177598385469224 33.89404641775984 23.58728557013118 45 27.02577246765828 17.640113826716988 33.61722739106743 21.716886314557307 46 26.981404312423695 18.426177239201284 29.028645228990303 25.563773219384718 47 31.69269887395568 19.847035557169956 28.081486862816323 20.378778706058036 48 31.302908918463512 20.709521839590753 24.416551473630044 23.571017768315695 49 29.341849037947355 22.542401953365417 24.595655376295262 23.520093632391966 50 30.129052438273785 22.411131403432655 23.509943798117185 23.949872360176375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 1.0 7 2.5 8 4.0 9 4.0 10 4.0 11 3.0 12 2.0 13 2.0 14 2.0 15 5.5 16 9.0 17 10.5 18 12.0 19 22.0 20 32.0 21 43.5 22 55.0 23 79.0 24 103.0 25 131.5 26 160.0 27 208.0 28 256.0 29 334.5 30 413.0 31 522.5 32 632.0 33 757.5 34 883.0 35 1071.5 36 1260.0 37 1426.5 38 1593.0 39 1785.5 40 1978.0 41 2237.5 42 2497.0 43 2799.5 44 3102.0 45 3481.0 46 3860.0 47 4142.5 48 4425.0 49 4973.0 50 5521.0 51 5976.0 52 6431.0 53 6553.5 54 6676.0 55 6866.5 56 7057.0 57 6955.5 58 6854.0 59 6665.0 60 6476.0 61 6183.5 62 5891.0 63 5625.0 64 5359.0 65 5039.5 66 4720.0 67 4516.5 68 4313.0 69 4073.5 70 3834.0 71 3544.5 72 3255.0 73 3069.5 74 2884.0 75 2590.5 76 2297.0 77 2136.5 78 1976.0 79 1682.5 80 1389.0 81 1197.0 82 1005.0 83 855.5 84 706.0 85 609.0 86 512.0 87 404.5 88 297.0 89 241.0 90 185.0 91 144.5 92 104.0 93 77.0 94 50.0 95 35.0 96 20.0 97 17.5 98 15.0 99 16.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10491485755790492 2 0.005043983536437737 3 0.0 4 0.0010087967072875475 5 0.0010087967072875475 6 0.0 7 0.0010087967072875475 8 0.0010087967072875475 9 0.0010087967072875475 10 0.0010087967072875475 11 0.002017593414575095 12 0.002017593414575095 13 0.009079170365587927 14 0.010087967072875475 15 0.011096763780163022 16 0.14425792914211927 17 0.010087967072875475 18 0.15535469292228232 19 0.18158340731175854 20 0.24312000645629891 21 0.25825195706561216 22 0.05447502219352756 23 0.017149544023888304 24 0.1150028246307804 25 0.05346622548624001 26 0.03228149463320152 27 0.03228149463320152 28 0.029255104511338878 29 0.037325478169639256 30 0.07364215963199096 31 0.045395851827939634 32 0.03934307158421435 33 0.041360664998789445 34 0.04741344524251473 35 0.0403518682915019 36 0.04438705512065209 37 0.037325478169639256 38 0.0403518682915019 39 0.04438705512065209 40 0.16645145670244532 41 0.04236946170607699 42 0.04337825841336454 43 0.03530788475506416 44 0.028246307804051327 45 0.030263901218626422 46 0.0191671374384634 47 0.02017593414575095 48 0.0191671374384634 49 0.017149544023888304 50 0.021184730853038496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 99128.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.66721814219999 #Duplication Level Percentage of deduplicated Percentage of total 1 98.73989012263664 94.46170607699138 2 0.660107768392859 1.2630134775240094 3 0.19191631605032003 0.5508030021790009 4 0.09068573176004134 0.3470260673069163 5 0.062214629928400454 0.2975950286498265 6 0.03901595436187825 0.2239528690178355 7 0.04007043961490199 0.2683399241384876 8 0.023198675566522203 0.17754822048260835 9 0.03374352809675957 0.29053345169881367 >10 0.11177543682051606 1.5091598741021708 >50 0.006326911518142419 0.4731256557178597 >100 0.0010544852530237365 0.13719635219110643 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 136 0.13719635219110643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.00807037365830038 0.0 2 0.0 0.0 0.0 0.009079170365587927 0.0 3 0.0 0.0 0.0 0.009079170365587927 0.0 4 0.0 0.0 0.0 0.013114357194738116 0.0 5 0.0 0.0 0.0 0.021184730853038496 0.0 6 0.0 0.0 0.0 0.021184730853038496 0.0 7 0.0 0.0 0.0 0.021184730853038496 0.0 8 0.0 0.0 0.0 0.021184730853038496 0.0 9 0.0 0.0 0.0 0.02320232426761359 0.0 10 0.0 0.0 0.0 0.024211120974901138 0.0 11 0.0 0.0 0.0 0.028246307804051327 0.0 12 0.0 0.0 0.0 0.028246307804051327 0.0 13 0.0 0.0 0.0 0.029255104511338875 0.0 14 0.0 0.0 0.0 0.03228149463320152 0.0 15 0.0 0.0 0.0 0.034299088047776614 0.0 16 0.0 0.0 0.0 0.03530788475506416 0.0 17 0.0 0.0 0.0 0.037325478169639256 0.0 18 0.0 0.0 0.0 0.03934307158421435 0.0 19 0.0 0.0 0.0 0.04236946170607699 0.0 20 0.0 0.0 0.0 0.06153659914454039 0.0 21 0.0 0.0 0.0 0.06355419255911549 0.0 22 0.0 0.0 0.0 0.07767734646114115 0.0 23 0.0 0.0 0.0 0.09482689048502946 0.0 24 0.0 0.0 0.0 0.11197643450891777 0.0 25 0.0 0.0 0.0 0.11298523121620531 0.0 26 0.0 0.0 0.0 0.11399402792349286 0.0 27 0.0 0.0 0.0 0.12105560487450569 0.0 28 0.0 0.0 0.0 0.1361875554838189 0.0 29 0.0 0.0 0.0 0.16342506658058267 0.0 30 0.0 0.0 0.0 0.17754822048260835 0.0 31 0.0 0.0 0.0 0.20075054475022194 0.0 32 0.0 0.0 0.0 0.2168912920668227 0.0 33 0.0 0.0 0.0 0.2471551932854491 0.0 34 0.0 0.0 0.0 0.3026390121862642 0.0 35 0.0 0.0 0.0 0.3742635784036801 0.0 36 0.0 0.0 0.0 0.45799370510854653 0.0 37 0.0 0.0 0.0 0.6244451618109919 0.0 38 0.0 0.0 0.0 0.7929142119280123 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAGAT 25 4.4334356E-5 43.966198 5 TATACAG 25 4.4334356E-5 43.966198 3 CTTTACA 85 0.0 38.83289 1 TTTACAC 105 0.0 35.591682 2 TCTTAAT 25 0.0023396392 35.208485 1 CTGATAC 25 0.0023396392 35.208485 1 ATTTCGG 25 0.0023454477 35.190712 42 TGATACA 25 0.0023512673 35.17296 2 GTCTGAC 25 0.0023512673 35.17296 7 TTATCAC 25 0.0023512673 35.17296 2 ATACAGA 25 0.0023512673 35.17296 4 CTTATGC 40 1.8003613E-5 33.007954 1 CTATACA 90 0.0 31.785439 1 CCTATAC 35 3.1922176E-4 31.436146 1 GCTCAGA 35 3.1922176E-4 31.436146 1 TTACACA 115 0.0 30.58518 3 GTTATAC 45 4.025743E-5 29.340405 1 TATCACA 30 0.005741452 29.310797 3 TTATACA 535 0.0 28.762932 2 GATACAC 40 7.017826E-4 27.478872 3 >>END_MODULE