FastQCFastQC Report
Wed 25 May 2016
SRR1780363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99128
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT3822738.56327172948107No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT2507825.298603825357112TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC72867.35009280929707No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG56885.73803567105157No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC38423.8757969493987567No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT23512.371681058833024No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC16001.614074731660076No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC12761.2872245984989106No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG10271.0360342183843112No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC8410.8483980308288274No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG7000.7061576951012832No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC6540.659753046566056No Hit
CTTAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC6150.6204099749818417No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4780.4822048260834477No Hit
CTTATCACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC3260.3288677265757405No Hit
CTTATTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG2990.30163021547897667No Hit
CTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT2720.27439270438221286No Hit
TCTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2590.2612783471874748No Hit
ACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTTCT2530.2552255669437495RNA PCR Primer, Index 18 (95% over 23bp)
ATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTTCTGCTT2250.2269792591396982TruSeq Adapter, Index 18 (96% over 27bp)
CCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG1990.20075054475022194No Hit
TCTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT1670.16846905011702043No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT1380.13921394560568154No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGC1320.13316116536195627No Hit
CTCTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG1220.1230731982890808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCA254.400521E-544.0217061
CTTATAA700.044.0217061
TCTTTAC254.400521E-544.0217061
TCTTAAT750.044.0217061
TAAACAT207.822107E-443.9994965
TTCTGCT454.7111826E-1043.99949643
TCTGCTT454.7111826E-1043.99949644
ATAAACA207.822107E-443.9994964
CTTCTGC454.7111826E-1043.99949642
TATAACA600.043.9994933
TTATCAC254.4136665E-543.9994932
TTATAAC551.8189894E-1243.9994932
ATAACAC600.043.9994934
CCGTCTT27300.042.46837644
CTTTACA7450.042.249021
TTTACAC7500.041.9461862
TCTTTAT1450.040.9857251
CTTAACA650.040.635421
TTAACAC650.040.6149182
TCTTCTG501.3224053E-939.59954541
Produced by FastQC (version 0.11.5)