FastQCFastQC Report
Wed 25 May 2016
SRR1780362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38619
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG150.002098630970.905511
ATCTCGG207.839284E-569.9870542
GTGTTTT150.002210228569.98704523
CGTATTA150.002210228569.98704557
ATTACGG150.002210228569.98704526
GGATCAT150.002210228569.98704531
CCATAGC150.002210228569.98704566
TATTACG150.002210228569.98704525
TTACGGA150.002210228569.98704527
GATCTCG252.792358E-669.98704541
GTTGTAT307.622817E-659.0879251
GAGTGTG200.006906553652.49028847
GTCGCCG200.006906553652.49028851
GTATTAT200.006906553652.49028858
GCTATGT200.006906553652.49028818
TTGTATA200.006906553652.4902882
AGGCGTA200.006906553652.49028854
CAACAAG200.006906553652.4902883
TACGGAT200.006906553652.49028828
TTTTTGA200.006906553652.49028849