##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780362_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38619 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.7300033662187 32.0 21.0 34.0 12.0 34.0 2 27.334446774903544 33.0 23.0 34.0 11.0 34.0 3 27.5884150288718 33.0 23.0 34.0 11.0 34.0 4 28.03363629301639 34.0 23.0 34.0 11.0 34.0 5 27.97931070198607 34.0 23.0 34.0 11.0 34.0 6 30.14430720629742 36.0 25.0 38.0 11.0 38.0 7 29.34868846940625 34.0 23.0 38.0 11.0 38.0 8 28.611175846086123 34.0 21.0 37.0 11.0 38.0 9 29.504026515445766 34.0 22.0 38.0 12.0 38.0 10-11 29.402392604676454 34.5 22.0 38.0 11.0 38.0 12-13 29.67086149304746 35.5 22.5 38.0 11.0 38.0 14-15 29.34657810922085 35.0 21.0 38.0 11.0 38.0 16-17 29.133224578575312 34.5 21.0 38.0 11.0 38.0 18-19 29.169929309407287 34.5 21.5 38.0 11.0 38.0 20-21 29.211968202180273 34.5 21.5 38.0 11.0 38.0 22-23 29.004104197415778 34.5 21.0 38.0 11.0 38.0 24-25 29.38174214764753 35.5 21.5 38.0 11.0 38.0 26-27 29.37340428286595 36.0 21.0 38.0 11.0 38.0 28-29 29.275227219762293 36.0 21.0 38.0 11.0 38.0 30-31 29.10768015743546 36.0 21.0 38.0 11.0 38.0 32-33 29.102410730469458 35.0 21.0 38.0 11.0 38.0 34-35 29.087677050156657 35.0 21.0 38.0 11.0 38.0 36-37 28.949403143530386 34.5 21.0 38.0 11.0 38.0 38-39 29.143931743442344 36.0 21.0 38.0 11.0 38.0 40-41 28.831546648022993 34.5 21.0 38.0 11.0 38.0 42-43 29.188145731375748 35.5 21.0 38.0 11.0 38.0 44-45 28.452070224500893 34.0 21.0 38.0 11.0 38.0 46-47 29.014604210362776 34.5 21.0 38.0 11.0 38.0 48-49 28.951979595535875 34.0 21.0 38.0 11.0 38.0 50-51 28.888862994898886 34.0 21.0 38.0 11.0 38.0 52-53 28.85490302700743 34.0 21.0 38.0 11.0 38.0 54-55 28.697363991817497 34.0 21.0 38.0 11.0 38.0 56-57 28.54589709728372 34.0 21.0 38.0 11.0 38.0 58-59 28.46148268986768 34.0 21.0 37.5 11.0 38.0 60-61 28.58589036484632 34.0 21.0 38.0 11.0 38.0 62-63 28.541236179082837 34.0 20.5 38.0 11.0 38.0 64-65 28.589696781377043 34.0 20.5 38.0 11.0 38.0 66-67 28.672039669592685 34.0 21.0 38.0 11.0 38.0 68-69 28.286516999404437 34.0 19.5 38.0 11.0 38.0 70-71 28.180507004324298 33.5 19.5 37.5 11.0 38.0 72-73 28.22274010202232 34.0 19.5 37.0 11.0 38.0 74-75 27.996297159429297 33.0 19.5 37.0 11.0 38.0 76 27.252129780677905 31.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.167439921934548 1101 2 -0.9194617547676209 1101 3 -1.000210501311006 1101 4 -1.6071075285337102 1101 5 -2.4849459158002816 1101 6 -3.194849325769674 1101 7 -1.2721474816992426 1101 8 -1.0075133294519176 1101 9 -2.271140187223608 1101 10-11 -2.1158972359417128 1101 12-13 -2.209266868033936 1101 14-15 -1.7406524640063772 1101 16-17 -1.641843151616719 1101 18-19 -1.8762317147476857 1101 20-21 -2.1575204592334636 1101 22-23 -2.5926073856398446 1101 24-25 -2.499835103112133 1101 26-27 -1.9652560240984194 1101 28-29 -2.8813959590734477 1101 30-31 -2.016871766862664 1101 32-33 -1.2920108805301673 1101 34-35 -1.4309812492099887 1101 36-37 -2.3200894329913737 1101 38-39 -1.4720341275336999 1101 40-41 -2.4175980019111627 1101 42-43 -1.2087793244297558 1101 44-45 -0.5274605986726151 1101 46-47 -1.433048159037373 1101 48-49 -0.9579593159776536 1101 50-51 -1.4202811752155782 1101 52-53 -1.314463235148132 1101 54-55 -1.3801664034058163 1101 56-57 -1.710869845951411 1101 58-59 -1.6164954871262296 1101 60-61 -2.155705302684801 1101 62-63 -1.9671561237354318 1101 64-65 -1.5365193477894188 1101 66-67 -1.4064649596635626 1101 68-69 -1.2592778413405128 1101 70-71 -0.8795865284974198 1101 72-73 -1.9855741427457936 1101 74-75 -2.587319314996556 1101 76 -2.977387840129758 1102 1 -0.2450608896764841 1102 2 -0.7806848730471891 1102 3 -1.4034363077626182 1102 4 -1.6632231199315584 1102 5 -0.2846770985959779 1102 6 -0.11655631501698593 1102 7 2.2080272494835533 1102 8 0.9612366705480824 1102 9 -0.24963481087952388 1102 10-11 0.19055437696151145 1102 12-13 -4.182385147603828 1102 14-15 -1.2801349908880972 1102 16-17 -1.2586106247349989 1102 18-19 -0.4264669298014496 1102 20-21 0.1860615300138484 1102 22-23 -0.7649864178979087 1102 24-25 -0.19455956547772146 1102 26-27 -4.432661938076915 1102 28-29 -1.0231970343422638 1102 30-31 -1.235453756109976 1102 32-33 -5.678771633218336 1102 34-35 -1.964919421252997 1102 36-37 -0.6939133577225576 1102 38-39 -0.7961268694691839 1102 40-41 0.09399474002432484 1102 42-43 -0.40501588356954343 1102 44-45 -5.525108448134983 1102 46-47 -0.9048223525857573 1102 48-49 -0.6163934557626014 1102 50-51 -4.075019078441386 1102 52-53 -0.8935962996642601 1102 54-55 0.2898604783146155 1102 56-57 -2.252200491112699 1102 58-59 -0.10574279895418215 1102 60-61 -0.7840655177385649 1102 62-63 -3.497230048466612 1102 64-65 -1.7552693477894152 1102 66-67 -1.1389918413839908 1102 68-69 -5.082530529512557 1102 70-71 -1.5793513134436559 1102 72-73 -1.6177986051113855 1102 74-75 -5.879490013921284 1102 76 -1.9874684852910498 1103 1 0.20179645495628407 1103 2 -0.6057201837816528 1103 3 0.24345634760461365 1103 4 -0.3745578656942712 1103 5 -0.5509200364490852 1103 6 -0.864614224621505 1103 7 -0.40373122509271653 1103 8 -0.8945189791694332 1103 9 0.0102521947701959 1103 10-11 -0.38430438010063384 1103 12-13 -1.0736280854569387 1103 14-15 -0.7032458665943153 1103 16-17 -1.4659729128705905 1103 18-19 -1.5120071840387368 1103 20-21 -0.15159736829123815 1103 22-23 -0.5931996947340679 1103 24-25 -1.0643512321443893 1103 26-27 -1.2527537459865172 1103 28-29 -0.024962571065429984 1103 30-31 -0.8575406205167546 1103 32-33 -3.91411767841608 1103 34-35 -0.9760423026089278 1103 36-37 -0.5734154763666268 1103 38-39 -0.519555541785568 1103 40-41 -1.0960652882242634 1103 42-43 -0.9530384824395988 1103 44-45 -4.822248278643457 1103 46-47 -1.2374494712298265 1103 48-49 -0.3498856874010201 1103 50-51 -0.6261313665769777 1103 52-53 -0.5289246894947688 1103 54-55 -0.18365647083792425 1103 56-57 -0.8173487961974466 1103 58-59 -1.5789066407620922 1103 60-61 -0.4652095855351739 1103 62-63 -1.765679907223678 1103 64-65 -1.4743724551340485 1103 66-67 -0.7117319543783438 1103 68-69 -2.6640983261227262 1103 70-71 -0.45991275412162125 1103 72-73 -0.6885083508740983 1103 74-75 -1.048363601491907 1103 76 -0.494883739528337 1104 1 -1.9560727944383878 1104 2 -0.040506301618620455 1104 3 -0.12248392681024001 1104 4 -1.0313778818363204 1104 5 -1.3239628128816925 1104 6 -0.5466158388265079 1104 7 -0.4303656076593043 1104 8 0.2496295276909386 1104 9 -0.5734443346890465 1104 10-11 -0.5380170516099163 1104 12-13 -0.530004195222876 1104 14-15 -0.4374266575547665 1104 16-17 -1.0307832437826185 1104 18-19 0.7352890225795008 1104 20-21 -0.2533729937956757 1104 22-23 -0.09445070361219265 1104 24-25 -0.06911313690629584 1104 26-27 1.244421395256417 1104 28-29 -0.2714113200565542 1104 30-31 -0.9305430418242615 1104 32-33 -1.5350216332183386 1104 34-35 -0.3408122783958554 1104 36-37 -0.14912169105588902 1104 38-39 -0.014430440897754693 1104 40-41 0.40575069240527384 1104 42-43 0.43069840214474553 1104 44-45 -1.9426679719445055 1104 46-47 0.017796695033290177 1104 48-49 -0.18499464623879192 1104 50-51 0.9471535406062301 1104 52-53 0.01994536700240701 1104 54-55 -0.025020474066337783 1104 56-57 -0.038212395874605676 1104 58-59 1.208542915331531 1104 60-61 -0.22513694630999126 1104 62-63 -0.1485693341809018 1104 64-65 0.6206235093534396 1104 66-67 0.18124625385410198 1104 68-69 -2.965268624750653 1104 70-71 -1.0339644086817508 1104 72-73 -0.15009027177805123 1104 74-75 -1.2034483472546214 1104 76 -0.595206580529144 1105 1 1.1929258704481285 1105 2 1.642893787388946 1105 3 -1.1869254043358275 1105 4 0.21090071184414327 1105 5 0.6152450197840835 1105 6 0.45694446379921416 1105 7 1.6230389317265441 1105 8 1.2510536487412267 1105 9 2.0525458744787315 1105 10-11 1.5908659034412622 1105 12-13 0.3144995875986609 1105 14-15 0.3136636851243608 1105 16-17 1.891068113582758 1105 18-19 1.0240295655256517 1105 20-21 0.08165148328487604 1105 22-23 0.05169971917374738 1105 24-25 0.6646207304724356 1105 26-27 0.48896968186078027 1105 28-29 0.2585397257823452 1105 30-31 0.0056385336096482774 1105 32-33 1.2335141923268331 1105 34-35 0.6013773077189661 1105 36-37 0.9122490254550115 1105 38-39 0.7582442364498192 1105 40-41 1.1948027618311734 1105 42-43 -0.35906572780629986 1105 44-45 1.0820761313042695 1105 46-47 0.6337290492833993 1105 48-49 0.3226155006236908 1105 50-51 -0.19432398498344128 1105 52-53 0.031783373232936896 1105 54-55 0.5404445904641477 1105 56-57 -0.10821684625288697 1105 58-59 0.18241919481528512 1105 60-61 0.5623129869343337 1105 62-63 0.7589518051159416 1105 64-65 -0.18916732286729854 1105 66-67 0.9610860402359442 1105 68-69 1.2907171340388466 1105 70-71 1.9631428454348487 1105 72-73 1.0231184509633842 1105 74-75 -0.019336197721909087 1105 76 0.5318072156435285 1106 1 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-1.5640761322875179 1106 60-61 -0.6165655177385645 1106 62-63 -0.3611883817999484 1106 64-65 -0.1431860144560808 1106 66-67 -0.4273251747173248 1106 68-69 0.41496947048744204 1106 70-71 0.11627368655634385 1106 72-73 -1.118423605111385 1106 74-75 -1.9434483472546198 1106 76 -1.589968485291049 1107 1 -0.440784573887008 1107 2 -0.28561908357350774 1107 3 -0.008699465657354466 1107 4 0.41298302041931834 1107 5 -0.7254665722801903 1107 6 0.39824631656196274 1107 7 -0.10831046981469328 1107 8 -0.09523262769753416 1107 9 -1.8461260389496985 1107 10-11 -0.6427789563718207 1107 12-13 0.8044569576593297 1107 14-15 -0.2847950786073987 1107 16-17 0.15229946298429908 1107 18-19 1.3715045614266188 1107 20-21 0.9116646001892867 1107 22-23 -0.5561048389505387 1107 24-25 0.5935435046977169 1107 26-27 -0.08014000825235357 1107 28-29 0.02395208846474972 1107 30-31 -0.9807936683906746 1107 32-33 0.8667327527465751 1107 34-35 0.30755864892244134 1107 36-37 -0.26929712965238295 1107 38-39 0.41138409544309695 1107 40-41 0.9972293891471296 1107 42-43 0.8581420111673026 1107 44-45 -0.4741216060297191 1107 46-47 -0.2973662122348806 1107 48-49 0.45899031616722397 1107 50-51 1.0009129391024736 1107 52-53 0.11380501612521243 1107 54-55 -0.6893061883520488 1107 56-57 1.0016372281855475 1107 58-59 0.7670642185896739 1107 60-61 0.5481713243666988 1107 62-63 0.004688811182507635 1107 64-65 -0.5277474179648571 1107 66-67 -0.3955707887524156 1107 68-69 0.6027764880313029 1107 70-71 0.6796947391879229 1107 72-73 0.47183955278335077 1107 74-75 -0.6078343121669008 1107 76 -0.6859333975717519 1108 1 -0.42712209911601207 1108 2 -1.5670971149350947 1108 3 -0.4816073992080483 1108 4 -1.2995616155067786 1108 5 -1.377228721014859 1108 6 -2.241095400562706 1108 7 -1.042249299189013 1108 8 -0.8246224739946904 1108 9 -1.0608442504075448 1108 10-11 -1.6427789563718207 1108 12-13 -1.1676358850669573 1108 14-15 -1.5306419967878035 1108 16-17 -2.5701427043810163 1108 18-19 -2.4277113988279986 1108 20-21 -2.435758160251641 1108 22-23 -2.784252642086699 1108 24-25 -2.5820503471886376 1108 26-27 -2.9841921740651145 1108 28-29 -2.228764880949935 1108 30-31 -1.0837668092073187 1108 32-33 -2.066585055047245 1108 34-35 -2.3650853504565355 1108 36-37 -1.5582662338287534 1108 38-39 -1.3666006895281804 1108 40-41 -1.7870432393267102 1108 42-43 -2.3261073289972707 1108 44-45 -2.176380572028787 1108 46-47 -1.2731851844441628 1108 48-49 -1.8597101961755804 1108 50-51 -2.3364958483528895 1108 52-53 -1.9617655474518685 1108 54-55 -1.7593651854021992 1108 56-57 -0.9582292521746467 1108 58-59 -2.2001380791901752 1108 60-61 -1.441698261101397 1108 62-63 -1.9014981163132205 1108 64-65 -2.7828320321551985 1108 66-67 -1.3473251747173265 1108 68-69 -1.7921544233178643 1108 70-71 -1.2440360479569286 1108 72-73 -1.635635994491917 1108 74-75 -2.2671651614139137 1108 76 -2.1672251224591896 1109 1 -0.12878836715395892 1109 2 -1.415538476650795 1109 3 -0.3358687401950533 1109 4 0.876472826014389 1109 5 0.3020346131157332 1109 6 1.4816981894875205 1109 7 -0.37502455231824783 1109 8 0.2897839678453771 1109 9 0.6873021260574141 1109 10-11 0.032896719303856514 1109 12-13 -0.5787815440002255 1109 14-15 0.21662739649928753 1109 16-17 -0.6306545436539182 1109 18-19 -0.33961445232397125 1109 20-21 -0.3610950015176826 1109 22-23 0.40477709561560715 1109 24-25 0.8680812002880423 1109 26-27 -0.052875902040877776 1109 28-29 -0.15776910641433872 1109 30-31 0.5020180907368683 1109 32-33 0.3992126010158934 1109 34-35 -0.5845703221538976 1109 36-37 0.48781524588104475 1109 38-39 0.021862995395682816 1109 40-41 -0.1277113410567594 1109 42-43 -0.07843930699296564 1109 44-45 -0.5152548445313805 1109 46-47 -0.08387640663981344 1109 48-49 -0.20352746477161077 1109 50-51 0.033511326964017485 1109 52-53 1.085711132768175 1109 54-55 0.8129460639002026 1109 56-57 0.02060356294135346 1109 58-59 0.30416711095572424 1109 60-61 0.44847952730647833 1109 62-63 0.09750531189374811 1109 64-65 0.07158876031868999 1109 66-67 0.4905126631205121 1109 68-69 0.4461856867036609 1109 70-71 0.57478720006986 1109 72-73 -0.25729747898526156 1109 74-75 0.31096606715979647 1109 76 0.5648963795738133 1110 1 0.22444668608109453 1110 2 0.16685300574068762 1110 3 0.42080611647980604 1110 4 1.0032541527957157 1110 5 0.14183805291917295 1110 6 1.2620421698314992 1110 7 -1.4914045686982647 1110 8 1.762183640245052 1110 9 -2.3829681442128567 1110 10-11 0.4435846799918153 1110 12-13 -0.16723363245231582 1110 14-15 0.3868157666876577 1110 16-17 0.17008255708318387 1110 18-19 -0.22163738434690572 1110 20-21 -0.5477452881679703 1110 22-23 0.3291423699808824 1110 24-25 0.799285131491974 1110 26-27 0.19139109222611594 1110 28-29 1.2355150868698566 1110 30-31 0.23698942570820591 1110 32-33 1.3134632152665127 1110 34-35 0.6799669423833663 1110 36-37 1.120575278641077 1110 38-39 1.8736647971974847 1110 40-41 1.3973091339637165 1110 42-43 1.064681086127429 1110 44-45 1.9402324609559258 1110 46-47 0.6792685565051535 1110 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1.7280803273752205 2116 46-47 1.2456878514958767 2116 48-49 0.14051555784284275 2116 50-51 0.9138202072728987 2116 52-53 1.0308297207439026 2116 54-55 -0.6399864604608965 2116 56-57 0.6145086925607721 2116 58-59 0.34663815342676685 2116 60-61 -1.5098308238610123 2116 62-63 0.7252401896286216 2116 64-65 1.5723241896255509 2116 66-67 -0.3269170114520179 2116 68-69 0.3728266133445892 2116 70-71 -1.5764814154844693 2116 72-73 0.4329029255008585 2116 74-75 -2.1136524288872707 2116 76 0.14901110654568583 2117 1 0.7325274941619 2117 2 0.6830146219023057 2117 3 0.813735409409098 2117 4 0.20325415279571502 2117 5 -0.530889219808099 2117 6 -0.6490689412796122 2117 7 -0.4823136596073567 2117 8 -0.3913517132903017 2117 9 0.16450660326189137 2117 10-11 -0.9458092594021252 2117 12-13 -0.0914760566947379 2117 14-15 -0.7970226171507271 2117 16-17 0.5458401328407589 2117 18-19 -0.19587980858932852 2117 20-21 -1.1174422578649406 2117 22-23 0.4013645922031017 2117 24-25 0.7386790708859152 2117 26-27 0.6282597790948046 2117 28-29 0.2829898343446011 2117 30-31 0.604666193384972 2117 32-33 1.2457864475897402 2117 34-35 0.8365325989490238 2117 36-37 -0.8582126001467998 2117 38-39 -0.2066382331055472 2117 40-41 -1.5314787448241631 2117 42-43 -1.06410679266045 2117 44-45 1.7397274104508753 2117 46-47 0.1519958292324226 2117 48-49 0.33578457454042976 2117 50-51 0.01194430539699809 2117 52-53 0.33509688215391975 2117 54-55 -1.0605183095641735 2117 56-57 0.027155569493363174 2117 58-59 -0.6612983545097393 2117 60-61 0.5802021590291098 2117 62-63 -0.5355823211938855 2117 64-65 0.3170160057459377 2117 66-67 -0.6200524474445999 2117 68-69 -0.06295982244185083 2117 70-71 0.7127888380714964 2117 72-73 0.4751622534744726 2117 74-75 -0.578195822002094 2117 76 -0.8757260610486242 2118 1 1.7349819452144821 2118 2 0.6055106098188539 2118 3 1.1027942218323972 2118 4 0.3791250109095614 2118 5 1.007768384270065 2118 6 0.8961968002478145 2118 7 1.950141735464861 2118 8 -2.7582921456512963 2118 9 0.5852561548525657 2118 10-11 -0.11006867599799008 2118 12-13 1.5154341670379168 2118 14-15 0.10805620848885056 2118 16-17 0.5406008238631301 2118 18-19 -0.0834470699883667 2118 20-21 -0.6333017877431608 2118 22-23 0.42553149487467934 2118 24-25 0.010415122995798498 2118 26-27 1.2179416444776052 2118 28-29 0.17442757624963434 2118 30-31 0.024330122394697895 2118 32-33 0.6447291455978608 2118 34-35 1.2275455320180306 2118 36-37 0.9916882777914608 2118 38-39 0.9358143299077639 2118 40-41 -0.3893093877015339 2118 42-43 0.05682212266098574 2118 44-45 1.2129172527995955 2118 46-47 0.5169066193768579 2118 48-49 0.09831643520313094 2118 50-51 0.628105921558614 2118 52-53 -0.4168147576081864 2118 54-55 0.07782776803423985 2118 56-57 1.7282317518779529 2118 58-59 0.6310173256564084 2118 60-61 -0.16198607848622615 2118 62-63 0.5253069453028587 2118 64-65 -0.12374676211963376 2118 66-67 -1.2678859223808772 2118 68-69 0.31408162002015416 2118 70-71 -0.7658291171819727 2118 72-73 -0.9021151938964316 2118 74-75 1.3077666060164077 2118 76 0.784143664241661 2119 1 0.47091133254573947 2119 2 1.365842904730588 2119 3 0.8632303589040475 2119 4 -0.2361397865982262 2119 5 0.23476734584846426 2119 6 -0.7228063150169852 2119 7 -0.8621116394053345 2119 8 0.6480422261036374 2119 9 0.11703185578714326 2119 10-11 -0.35944562303848926 2119 12-13 0.3509481857295036 2119 14-15 0.7625733424452328 2119 16-17 -0.2228467358461117 2119 18-19 -1.239314152023674 2119 20-21 -2.4740079144305973 2119 22-23 0.12459691543542561 2119 24-25 -0.3754623432554993 2119 26-27 -0.9333563825213602 2119 28-29 0.23652518787995547 2119 30-31 -0.6503843116655332 2119 32-33 0.4149783667816642 2119 34-35 -0.31700275458632987 2119 36-37 -1.149121691055889 2119 38-39 -0.6930018694691817 2119 40-41 -0.4744080377534523 2119 42-43 -1.2883492169028763 2119 44-45 -1.6188584481349828 2119 46-47 -1.3353779081413144 2119 48-49 -1.127851789095935 2119 50-51 0.4836614771141683 2119 52-53 -1.2467212996642587 2119 54-55 -2.7115284105742745 2119 56-57 0.033216175553967275 2119 58-59 0.9053683121569271 2119 60-61 -0.007676628849676348 2119 62-63 -0.9906328262443935 2119 64-65 -0.8142971255671938 2119 66-67 -1.163991841383993 2119 68-69 -1.625586085068111 2119 70-71 -0.8276152023325452 2119 72-73 -2.23897916066694 2119 74-75 -0.1423372361435078 2119 76 -1.011079596402162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 17.0 13 143.0 14 548.0 15 950.0 16 1428.0 17 1773.0 18 1999.0 19 1934.0 20 1721.0 21 1293.0 22 1002.0 23 751.0 24 636.0 25 582.0 26 564.0 27 559.0 28 717.0 29 751.0 30 876.0 31 1033.0 32 1375.0 33 1703.0 34 2295.0 35 3276.0 36 5265.0 37 5427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.289199332777315 23.608215179316097 10.276793160967474 37.825792326939116 2 13.972397006654754 21.89336854915974 31.660581579015513 32.473652865169996 3 12.467956187368912 25.45120277583573 27.105828737150105 34.97501229964525 4 9.415054765788861 28.162303529350837 31.106450192910224 31.316191511950077 5 8.897174965690462 41.6478935239131 29.45700302959683 19.997928480799608 6 18.576348429529506 37.02063751003392 15.147984152878117 29.255029907558455 7 17.7063103653642 31.862554701053885 18.993241668608714 31.437893264973198 8 16.42714725912116 35.78549418679924 20.611616043916207 27.175742510163392 9 18.38732230249359 21.83899117014941 18.654030399544265 41.11965612781274 10-11 18.547865040524094 27.820502861285895 25.878453610916907 27.753178487273107 12-13 17.993733654418808 25.34115331831482 24.847872808721096 31.817240218545273 14-15 18.21901136746161 27.555089463735467 21.65773324011497 32.568165928687954 16-17 19.881405525777467 28.58955436443201 22.94984334136047 28.579196768430048 18-19 18.595768922033194 27.429503612211604 23.267044718920737 30.707682746834458 20-21 17.664879981356325 27.930552318806807 23.513037623967477 30.89153007586939 22-23 20.887387037468606 27.84251275279008 22.411248349258138 28.85885186048318 24-25 19.063155441621998 29.617545767627334 22.378880861751988 28.94041792899868 26-27 17.653227685854116 30.65589476682462 24.212175354100314 27.478702193220954 28-29 19.29231725316554 27.767420181775808 26.575001942049248 26.3652606230094 30-31 19.297496051166522 27.943499313809266 26.577591341049743 26.18141329397447 32-33 17.48621145032238 30.671431160827574 25.210388668789975 26.631968720060073 34-35 18.49478236101401 29.414277946088713 24.770190838706334 27.320748854190942 36-37 18.76019575856444 28.114399647841736 25.47968616484114 27.645718428752687 38-39 18.335534322483753 26.628084621559335 27.297444263186517 27.738936792770396 40-41 17.53152593283099 27.455397602216525 26.551697351044822 28.461379113907665 42-43 15.830290789507757 26.50638286853621 28.254227193868303 29.40909914808773 44-45 15.937750848028173 26.967295890623788 28.39405473989487 28.700898521453173 46-47 16.721044045676997 27.9642145058132 27.656076024754654 27.658665423755146 48-49 16.22258473808229 29.22136772055206 25.882337709417648 28.673709831948003 50-51 16.094409487557936 28.179134622854036 25.501696056345324 30.224759833242704 52-53 15.880784070017349 27.924078821305574 26.79251145809058 29.4026256505865 54-55 15.393977057924857 28.546829280923898 25.317848727310395 30.741344933840853 56-57 16.56309070664699 28.26199539086978 24.924260079235612 30.250653823247625 58-59 16.780600222688314 27.839923353789587 24.925554778735854 30.45392164478625 60-61 15.880784070017349 27.256013879178642 24.994173852248895 31.869028198555117 62-63 16.450451850125585 27.524016675729563 24.405085579636967 31.62044589450789 64-65 17.275175431782284 28.158419430850103 22.892876563349645 31.673528574017972 66-67 16.478935239130998 30.180740050234338 22.185970636215334 31.154354074419327 68-69 16.783189621688805 30.172971853232866 22.259768507729355 30.784070017348974 70-71 17.135347885755717 30.044796602708512 22.419016546259613 30.40083896527616 72-73 17.233745047774413 29.991713923198425 21.449286620575364 31.325254408451798 74-75 17.446075765814754 30.45392164478625 21.55674667909578 30.54325591030322 76 17.1573577772599 29.92827364768637 21.61889225510759 31.295476319946143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 1.5 10 1.5 11 1.5 12 1.0 13 0.5 14 2.5 15 3.5 16 5.0 17 8.0 18 9.5 19 13.0 20 14.5 21 14.0 22 17.0 23 33.5 24 52.0 25 57.0 26 70.0 27 114.0 28 160.0 29 175.0 30 214.5 31 270.5 32 389.5 33 492.0 34 564.5 35 748.0 36 938.0 37 1017.0 38 1174.0 39 1473.5 40 1750.0 41 2022.0 42 2160.0 43 2278.0 44 2539.5 45 2662.5 46 2642.0 47 2655.5 48 2682.0 49 2520.5 50 2346.0 51 2276.5 52 2038.0 53 1756.5 54 1644.0 55 1515.5 56 1295.5 57 1059.0 58 914.0 59 822.0 60 639.5 61 461.0 62 373.0 63 340.0 64 266.0 65 203.0 66 154.5 67 128.0 68 106.0 69 74.0 70 50.5 71 37.0 72 30.0 73 23.5 74 19.0 75 14.0 76 17.0 77 15.0 78 11.0 79 12.0 80 9.0 81 5.5 82 6.5 83 8.0 84 5.0 85 2.0 86 3.5 87 5.0 88 3.0 89 1.5 90 2.0 91 2.5 92 3.0 93 2.5 94 3.5 95 3.5 96 2.0 97 2.0 98 4.5 99 5.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6499391491234885 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 38619.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.65509205313447 #Duplication Level Percentage of deduplicated Percentage of total 1 86.94168129510435 69.25347626815817 2 7.3532280085820165 11.714441078225745 3 2.828164618685391 6.758331391284083 4 1.365320850399844 4.3501903208265364 5 0.6078928548208828 2.4210880654600064 6 0.39659319940185944 1.8954400683601336 7 0.18204278005331254 1.0150444081928585 8 0.11377673753332034 0.7250317201377561 9 0.07476757037903907 0.536005593101841 >10 0.1365320850399844 1.3309510862528806 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01294699500245993 0.0 2 0.0 0.0 0.0 0.01294699500245993 0.0 3 0.0 0.0 0.0 0.01294699500245993 0.0 4 0.0 0.0 0.0 0.015536394002951915 0.0 5 0.0 0.0 0.0 0.015536394002951915 0.0 6 0.0 0.0 0.0 0.015536394002951915 0.0 7 0.0 0.0 0.0 0.015536394002951915 0.0 8 0.0 0.0 0.0 0.015536394002951915 0.0 9 0.0 0.0 0.0 0.015536394002951915 0.0 10 0.0 0.0 0.0 0.015536394002951915 0.0 11 0.0 0.0 0.0 0.015536394002951915 0.0 12 0.0 0.0 0.0 0.015536394002951915 0.0 13 0.0 0.0 0.0 0.015536394002951915 0.0 14 0.0 0.0 0.0 0.015536394002951915 0.0 15 0.0 0.0 0.0 0.0181257930034439 0.0 16 0.0 0.0 0.0 0.0181257930034439 0.0 17 0.0 0.0 0.0 0.02330459100442787 0.0 18 0.0 0.0 0.0 0.02589399000491986 0.0 19 0.0 0.0 0.0 0.03107278800590383 0.0 20 0.0 0.0 0.0 0.0362515860068878 0.0 21 0.0 0.0 0.0 0.04660918200885574 0.0 22 0.0 0.0 0.0 0.056966778010823685 0.0 23 0.0 0.0 0.0 0.056966778010823685 0.0 24 0.0 0.0 0.0 0.06473497501229965 0.0 25 0.0 0.0 0.0 0.06732437401279164 0.0 26 0.0 0.0 0.0 0.06732437401279164 0.0 27 0.0 0.0 0.0 0.09062896501721951 0.0 28 0.0 0.0 0.0 0.11652295502213936 0.0 29 0.0 0.0 0.0 0.14241694502705923 0.0 30 0.0 0.0 0.0 0.16054273803050312 0.0 31 0.0 0.0 0.0 0.17348973303296306 0.0 32 0.0 0.0 0.0 0.21492011704083483 0.0 33 0.0 0.0 0.0 0.26411869805018257 0.0 34 0.0 0.0 0.0 0.33662187006395816 0.0 35 0.0 0.0 0.0 0.37287345607084593 0.0 36 0.0 0.0 0.0 0.5049328050959372 0.0 37 0.0 0.0 0.0 0.6810119371293922 0.0 38 0.0 0.0 0.0 0.9658458271835108 0.0 39 0.0 0.0 0.0 1.2688055102410731 0.0 40 0.0 0.0 0.0 1.5847121883010953 0.0 41 0.0 0.0 0.0 1.9135658613635775 0.0 42 0.0 0.0 0.0 2.351174292446723 0.0 43 0.0 0.0 0.0 2.6722597685077294 0.0 44 0.0 0.0 0.0 3.052901421580051 0.0 45 0.0 0.0 0.0 3.467205261658769 0.0 46 0.0 0.0 0.0 3.9928532587586423 0.0 47 0.0 0.0 0.0 4.38126310883244 0.0 48 0.0 0.0 0.0 4.780030554908206 0.0 49 0.0 0.0 0.0 5.145135813977576 0.0 50 0.0 0.0 0.0 5.473989487040058 0.0 51 0.0 0.0 0.0 5.82873715010746 0.0 52 0.0 0.0 0.0 6.214557601180766 0.0 53 0.0 0.0 0.0 6.587431057251612 0.0 54 0.0 0.0 0.0 6.93699992231803 0.0 55 0.0 0.0 0.0 7.255495999378544 0.0 56 0.0 0.0 0.0 7.599886066443978 0.0 57 0.0 0.0 0.0 7.920971542504985 0.0 58 0.0 0.0 0.0 8.26536160957042 0.0 59 0.0 0.0 0.0 8.54242730262306 0.0 60 0.0 0.0 0.0 8.873870374686035 0.0 61 0.0 0.0 0.0 9.223439239752453 0.0 62 0.0 0.0 0.0 9.510862528807063 0.0 63 0.0 0.0 0.0 9.839716201869546 0.0 64 0.0 0.0 0.0 10.207410859939408 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAG 15 0.0020986309 70.90551 1 ATCTCGG 20 7.839284E-5 69.98705 42 GTGTTTT 15 0.0022102285 69.987045 23 CGTATTA 15 0.0022102285 69.987045 57 ATTACGG 15 0.0022102285 69.987045 26 GGATCAT 15 0.0022102285 69.987045 31 CCATAGC 15 0.0022102285 69.987045 66 TATTACG 15 0.0022102285 69.987045 25 TTACGGA 15 0.0022102285 69.987045 27 GATCTCG 25 2.792358E-6 69.987045 41 GTTGTAT 30 7.622817E-6 59.087925 1 GAGTGTG 20 0.0069065536 52.490288 47 GTCGCCG 20 0.0069065536 52.490288 51 GTATTAT 20 0.0069065536 52.490288 58 GCTATGT 20 0.0069065536 52.490288 18 TTGTATA 20 0.0069065536 52.490288 2 AGGCGTA 20 0.0069065536 52.490288 54 CAACAAG 20 0.0069065536 52.490288 3 TACGGAT 20 0.0069065536 52.490288 28 TTTTTGA 20 0.0069065536 52.490288 49 >>END_MODULE