FastQCFastQC Report
Wed 25 May 2016
SRR1780362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38619
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT29767.70605142546415No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT28657.41862813640954No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC9562.475465444470338No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC4641.2014811362282813No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG2910.7535151091431679No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT2560.6628861441259484No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT1670.43242963308216165No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1590.41171444107822575No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT1170.3029596830575623No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1150.2977808850565784No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT1120.29001268805510244No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT1080.2796550920531345No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1050.27188689505165853No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG1040.26929749605116654No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC930.24081410704575468No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT860.2226883140423108No Hit
TTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC710.18384732903493098No Hit
CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC660.17090033403247107No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT590.15277454102902716No Hit
CTTATTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG500.1294699500245993No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG490.1268805510241073No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTGGGATCTCGTATGCCGT460.11911235402263134No Hit
CTTATACACATCTCCGAGCCCACGCGACAGGTTGGGATCTCGTATGCCGT390.10098656101918745No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA150.002208606370.000018
ATTACAC207.8321056E-569.999994
CTTATAA207.8321056E-569.999991
TTTACAC1700.063.823532
CTTTACA1750.060.01
CTATACA900.058.333331
TATTACA252.3621052E-456.0000043
TTTATAC200.00690150352.4999963
TTACACA2100.051.6666643
CTTAATA305.808687E-446.6666682
TTAATAC350.001240755540.03
GAACAAA552.940566E-431.81818460
CACGAGC450.004257571431.11110921
ACGAGCC450.004257571431.11110922
TAATACA450.004257571431.1111094
ATATCGT450.004257571431.11110934
TTGGGAC703.4051493E-530.032
TGAACAA604.896664E-429.16666859
TGGGACC604.896664E-429.16666833
GGACCTC500.00712571528.00000232
Produced by FastQC (version 0.11.5)