##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38619 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.896449933970324 34.0 34.0 34.0 31.0 34.0 2 33.40021750951604 34.0 34.0 34.0 33.0 34.0 3 33.512545638157384 34.0 34.0 34.0 34.0 34.0 4 33.540744193272744 34.0 34.0 34.0 34.0 34.0 5 33.525544421139855 34.0 34.0 34.0 34.0 34.0 6 37.31500038840985 38.0 38.0 38.0 36.0 38.0 7 36.9257878246459 38.0 37.0 38.0 35.0 38.0 8 36.929775499106654 38.0 37.0 38.0 35.0 38.0 9 36.68857298221083 38.0 37.0 38.0 34.0 38.0 10-11 36.413462285403554 38.0 37.0 38.0 34.0 38.0 12-13 36.239376990600476 38.0 37.0 38.0 34.0 38.0 14-15 36.37060773194541 38.0 37.0 38.0 34.0 38.0 16-17 36.441816204458945 38.0 37.0 38.0 34.0 38.0 18-19 36.4837126802869 38.0 37.0 38.0 34.0 38.0 20-21 36.267679121675855 38.0 37.0 38.0 33.5 38.0 22-23 36.34029881664466 38.0 37.0 38.0 33.5 38.0 24-25 35.41014785468293 38.0 35.5 38.0 29.0 38.0 26-27 35.61847795126751 38.0 36.0 38.0 31.0 38.0 28-29 35.37649861467153 38.0 36.0 38.0 29.0 38.0 30-31 35.09484968538802 37.0 35.5 38.0 29.0 38.0 32-33 35.510448224966986 38.0 36.0 38.0 31.0 38.0 34-35 35.08025842202025 38.0 36.0 38.0 28.5 38.0 36-37 35.04111965612781 38.0 35.5 38.0 27.0 38.0 38-39 34.23518216421969 37.0 34.0 38.0 25.0 38.0 40-41 34.68786089748569 37.0 34.5 38.0 26.5 38.0 42-43 35.58041378596028 37.5 36.0 38.0 32.0 38.0 44-45 35.79152748647039 38.0 36.5 38.0 32.0 38.0 46-47 36.00086744866516 38.0 37.0 38.0 32.5 38.0 48-49 35.70245216085347 38.0 36.5 38.0 31.5 38.0 50-51 35.75484864962842 38.0 36.5 38.0 31.0 38.0 52-53 35.62620730728398 38.0 36.0 38.0 31.0 38.0 54-55 35.89215153162951 38.0 37.0 38.0 32.0 38.0 56-57 35.86248996607887 38.0 37.0 38.0 31.5 38.0 58-59 35.770061368756316 38.0 37.0 38.0 31.5 38.0 60-61 35.568580232528035 38.0 36.5 38.0 30.0 38.0 62-63 36.0163002667081 38.0 37.0 38.0 33.0 38.0 64-65 35.995610968694166 38.0 37.0 38.0 32.5 38.0 66-67 34.875255703151296 38.0 35.5 38.0 27.0 38.0 68-69 32.851925218156865 37.0 32.0 38.0 20.0 38.0 70-71 31.481887153991558 37.0 28.0 38.0 11.0 38.0 72-73 30.08173437945053 36.5 23.0 38.0 11.0 38.0 74-75 30.237978715140216 37.0 23.5 38.0 11.0 38.0 76 29.99383723037883 36.0 23.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5619746574107651 1101 2 0.5654171630776119 1101 3 0.47093162653005294 1101 4 0.4231638206390471 1101 5 0.4753293326609338 1101 6 -1.0565640107597005 1101 7 -0.01797098354715132 1101 8 0.6558803535688682 1101 9 0.8773315259281134 1101 10-11 0.47432624004137125 1101 12-13 0.5316549470914751 1101 14-15 0.945865068739117 1101 16-17 0.46365288556904716 1101 18-19 0.15287700682369376 1101 20-21 0.40216439026607276 1101 22-23 1.0790486250991762 1101 24-25 -1.3759564053895659 1101 26-27 -0.5945311140881628 1101 28-29 1.5497195011373393 1101 30-31 0.9704291842771369 1101 32-33 0.2886173941366792 1101 34-35 0.48775268822083007 1101 36-37 0.6048669125891522 1101 38-39 0.2570981480700496 1101 40-41 1.4617895677284807 1101 42-43 1.033414111969563 1101 44-45 0.5039090133189745 1101 46-47 0.9142356952513921 1101 48-49 -1.0720581829119595 1101 50-51 -1.1588620714264977 1101 52-53 -1.4185402420497937 1101 54-55 -0.353478836065662 1101 56-57 -1.039538740374887 1101 58-59 -0.9502400638525401 1101 60-61 -1.4630659372193122 1101 62-63 -0.694398264066102 1101 64-65 -0.6042105650782332 1101 66-67 -1.1012741637626178 1101 68-69 -2.2927896873104707 1101 70-71 -2.462800777831557 1101 72-73 -3.602948519029429 1101 74-75 -2.8532370473647966 1101 76 -1.660001826033234 1102 1 -0.48473233183654685 1102 2 0.31743329210986815 1102 3 -0.10030493260973117 1102 4 0.43559661633796765 1102 5 0.34024868749963844 1102 6 0.3846322257994359 1102 7 0.03881665086144892 1102 8 0.6189179879774684 1102 9 0.5399659345302652 1102 10-11 1.0717724766005077 1102 12-13 0.9226159685968511 1102 14-15 0.12479652035202093 1102 16-17 0.34302116513893566 1102 18-19 -0.5050531007031864 1102 20-21 0.623266540803705 1102 22-23 0.9142300767120872 1102 24-25 -4.878308555927202 1102 26-27 -1.289591597959138 1102 28-29 0.6123875118900273 1102 30-31 1.7740246143846647 1102 32-33 0.5329050285452865 1102 34-35 0.6940698925219024 1102 36-37 -2.52903765730332 1102 38-39 -0.6100053465536064 1102 40-41 1.559235804287617 1102 42-43 1.3838845420770909 1102 44-45 1.179984282136175 1102 46-47 1.233960157616984 1102 48-49 -3.5408081829119595 1102 50-51 -0.2962948671254253 1102 52-53 0.0196318009609584 1102 54-55 0.08754938974079352 1102 56-57 0.7193658295175851 1102 58-59 -0.09254517137942031 1102 60-61 -6.140317281305329 1102 62-63 -2.8701375113779335 1102 64-65 0.24928405857767189 1102 66-67 1.521709707205126 1102 68-69 -1.446687536772835 1102 70-71 -2.537733573530481 1102 72-73 -4.670992873868137 1102 74-75 -2.4409386602680208 1102 76 -1.0807007507644144 1103 1 0.27921540827644975 1103 2 0.18078074973698932 1103 3 -0.1363218817622709 1103 4 0.20801893272214755 1103 5 0.3250650716804344 1103 6 0.40634832749434935 1103 7 0.8105327525563624 1103 8 -0.029740204112925994 1103 9 0.14377949385229982 1103 10-11 0.4570125895948607 1103 12-13 -0.24290309919975783 1103 14-15 0.3072647406910036 1103 16-17 0.5610649504496763 1103 18-19 0.6654977467544398 1103 20-21 0.03534281199014089 1103 22-23 0.4856460371640594 1103 24-25 -4.302920137848105 1103 26-27 -0.9621728126483973 1103 28-29 -0.275412629352914 1103 30-31 1.1148614787914468 1103 32-33 1.629832994646982 1103 34-35 0.7791687625784007 1103 36-37 -1.1998533352694238 1103 38-39 -0.09332102451971025 1103 40-41 0.22696179298818464 1103 42-43 -0.190797661312736 1103 44-45 1.2357752425881543 1103 46-47 0.8855314853006035 1103 48-49 -3.3785553580532053 1103 50-51 -0.7911748106282488 1103 52-53 0.24985778966152594 1103 54-55 -0.0979202430275734 1103 56-57 0.5363679481616543 1103 58-59 0.03271965912905017 1103 60-61 -4.443018552491772 1103 62-63 -0.9550598277621134 1103 64-65 0.5875608947358728 1103 66-67 -0.0739823831903621 1103 68-69 -0.07062821473893877 1103 70-71 0.07861529652601718 1103 72-73 -3.668785952964182 1103 74-75 -2.8263553269346886 1103 76 -3.7918589428548124 1104 1 -0.36122042707464175 1104 2 0.05850472068129875 1104 3 0.15326649596169517 1104 4 -0.15041528842393603 1104 5 -0.361537026786074 1104 6 0.08076317818039058 1104 7 0.5135190318138285 1104 8 0.37784655940604495 1104 9 0.8816326011969338 1104 10-11 -0.526441809113777 1104 12-13 0.32812192097780013 1104 14-15 0.3288143774948793 1104 16-17 0.31772354609132236 1104 18-19 0.532446899296815 1104 20-21 0.020290350327513806 1104 22-23 0.1096169814739909 1104 24-25 0.2773462059775582 1104 26-27 1.3397238782313465 1104 28-29 1.5931910833185938 1104 30-31 0.35214961438466474 1104 32-33 0.5486788380690939 1104 34-35 0.13752227347428914 1104 36-37 1.2246825807919208 1104 38-39 1.2764529867797307 1104 40-41 1.484831042382858 1104 42-43 0.10561073255328068 1104 44-45 0.7192699964218932 1104 46-47 0.6692280147598382 1104 48-49 -0.1339629448167301 1104 50-51 -0.04718772426828366 1104 52-53 0.3601079914371539 1104 54-55 -0.6691470388306371 1104 56-57 -0.19744964667289366 1104 58-59 -1.542098742807994 1104 60-61 0.136319623456572 1104 62-63 0.05813629814588239 1104 64-65 0.807617391911009 1104 66-67 -0.9276950546996403 1104 68-69 1.758669606084304 1104 70-71 0.5488735693266626 1104 72-73 0.038679745179482694 1104 74-75 0.059358958779597515 1104 76 -0.31284360790727206 1105 1 0.9458556743939823 1105 2 0.39628843229678523 1105 3 0.41254553467998534 1105 4 0.26338088425074346 1105 5 0.2430913666273682 1105 6 0.4480083940237378 1105 7 0.7961180527306055 1105 8 -0.7854823235490116 1105 9 0.8542182709788619 1105 10-11 0.9103628192796407 1105 12-13 0.025273678877226757 1105 14-15 0.26980236147351633 1105 16-17 0.21558736451589056 1105 18-19 0.7128207310725188 1105 20-21 0.5257198118317419 1105 22-23 0.8110856063071026 1105 24-25 1.1469056185276258 1105 26-27 1.1479570780533308 1105 28-29 1.1298622003635401 1105 30-31 1.660560829337932 1105 32-33 0.8877531593864063 1105 34-35 -0.6748942819329216 1105 36-37 0.6921948193321938 1105 38-39 -0.04005791664705782 1105 40-41 -0.08192463184945353 1105 42-43 0.3123308037593304 1105 44-45 1.1336447182732527 1105 46-47 1.0114621046574825 1105 48-49 0.06034057098212031 1105 50-51 0.9355003042141448 1105 52-53 0.9129339816463258 1105 54-55 0.8450941716722511 1105 56-57 0.8264043809194561 1105 58-59 0.5863391744149737 1105 60-61 1.1063240738348554 1105 62-63 0.04331653846630701 1105 64-65 0.8400161458050945 1105 66-67 0.6955804237160237 1105 68-69 0.21861620154492556 1105 70-71 0.052122345472636056 1105 72-73 -0.328654478229506 1105 74-75 -0.23757028020571624 1105 76 -1.30928330528155 1106 1 -1.2826489985032126 1106 2 -0.18006670789013413 1106 3 0.14469506739026627 1106 4 -0.11232005032869807 1106 5 0.04608202083297641 1106 6 0.20838222579943277 1106 7 -1.317433349138554 1106 8 -0.020248678689192445 1106 9 -0.7250340654697354 1106 10-11 -0.2623941900661606 1106 12-13 1.085740968596852 1106 14-15 -0.3783284796479762 1106 16-17 -0.011562168194387823 1106 18-19 0.2474468992968113 1106 20-21 0.5357665408036993 1106 22-23 0.4281884100454221 1106 24-25 -0.8912252225938673 1106 26-27 0.5952000687075341 1106 28-29 0.19009584522335388 1106 30-31 0.02214961438466645 1106 32-33 0.12915502854528427 1106 34-35 0.6882365591885744 1106 36-37 1.0115873426966857 1106 38-39 0.01811965344639077 1106 40-41 0.4234024709542865 1106 42-43 0.7651345420770923 1106 44-45 0.6583176154695138 1106 46-47 0.8487518242836529 1106 48-49 1.3312751504213693 1106 50-51 -0.07837820045875787 1106 52-53 0.10629846762763151 1106 54-55 -0.09724227692587561 1106 56-57 0.1337408295175848 1106 58-59 0.659329828620578 1106 60-61 0.46655771869467344 1106 62-63 0.8952791552887405 1106 64-65 -1.644049274755659 1106 66-67 0.017543040538448906 1106 68-69 1.3908124632271637 1106 70-71 -1.3673169068638131 1106 72-73 -0.9887012072014691 1106 74-75 -1.3913553269346863 1106 76 -0.8523674174310827 1107 1 0.7340176681634532 1107 2 0.007126274566005009 1107 3 0.10364243581132371 1107 4 0.24206591458358417 1107 5 -0.1191811370617657 1107 6 0.08803134860645656 1107 7 0.6325666508614489 1107 8 -0.5093714857067368 1107 9 -1.0762621356451731 1107 10-11 0.4917286169513915 1107 12-13 0.0204778107021113 1107 14-15 -0.15727584806903394 1107 16-17 0.8446659019810454 1107 18-19 0.897359179998567 1107 20-21 0.7888367162423009 1107 22-23 -0.054794046094933435 1107 24-25 -0.14859364364649963 1107 26-27 -0.14348414181878155 1107 28-29 0.5857975996093217 1107 30-31 0.194254877542555 1107 32-33 1.2724883618786151 1107 34-35 0.49341199778506706 1107 36-37 1.311148746205447 1107 38-39 1.2290845657270921 1107 40-41 0.6458586113051652 1107 42-43 0.8888187526034059 1107 44-45 0.7084930540660039 1107 46-47 1.215593929546806 1107 48-49 1.1209242732283897 1107 50-51 1.1143410977868626 1107 52-53 1.2170002220135956 1107 54-55 0.6059156178109646 1107 56-57 0.6006706540789821 1107 58-59 0.9258210566907579 1107 60-61 1.2815577186946712 1107 62-63 0.9801914359904913 1107 64-65 1.0584945848934666 1107 66-67 0.483332514222667 1107 68-69 -0.03769630870265672 1107 70-71 -1.1062642752848646 1107 72-73 0.5398075647283527 1107 74-75 -0.19477637956626737 1107 76 0.2986852141478664 1108 1 -0.2423835117775468 1108 2 0.19630497352579823 1108 3 -0.01512794145929064 1108 4 0.2044941089633383 1108 5 -0.08028966058295595 1108 6 0.07351496916226807 1108 7 0.0883188632508265 1108 8 -0.48856726275999307 1108 9 -0.15140574688566488 1108 10-11 0.07490669488959867 1108 12-13 1.242997605764991 1108 14-15 0.38406090088299294 1108 16-17 -0.33904004430058876 1108 18-19 0.47226990814636594 1108 20-21 1.2222709655824602 1108 22-23 0.19571053393922 1108 24-25 1.5974473437778087 1108 26-27 0.0602885642827502 1108 28-29 -0.736939553006728 1108 30-31 0.3167513842961682 1108 32-33 0.9435798073063495 1108 34-35 0.23739585122397244 1108 36-37 1.2874722984488898 1108 38-39 0.8873674410570089 1108 40-41 0.8957033559100367 1108 42-43 -0.4388477588078672 1108 44-45 1.1560167305137625 1108 46-47 0.9566279304783336 1108 48-49 1.270124707943495 1108 50-51 1.0199846313996517 1108 52-53 -0.15219710759361504 1108 54-55 -0.07538386984623457 1108 56-57 0.4681213604910397 1108 58-59 0.9886218640188105 1108 60-61 1.186424975331839 1108 62-63 0.9046596862621925 1108 64-65 0.6869684243593923 1108 66-67 1.3047111821313777 1108 68-69 0.6689098083599063 1108 70-71 -2.124662039607177 1108 72-73 -1.9190109417147418 1108 74-75 -2.742284530474514 1108 76 -1.432101930705418 1109 1 -1.9326489985032111 1109 2 0.20650986868644594 1109 3 0.38955993225513197 1109 4 0.3435358055271607 1109 5 0.17076670551765716 1109 6 -0.14549164807444015 1109 7 -0.308874790579992 1109 8 0.3469585285180088 1109 9 0.7753262948906254 1109 10-11 0.5610742784023159 1109 12-13 0.3532184460743295 1109 14-15 0.7707706194511204 1109 16-17 0.8151495435173217 1109 18-19 0.4504649173148323 1109 20-21 0.5224782525154197 1109 22-23 0.2012514731084778 1109 24-25 -0.20798197935062746 1109 26-27 -0.5977278592203916 1109 28-29 -1.3367960466685318 1109 30-31 0.6314288936639443 1109 32-33 0.975191064581324 1109 34-35 0.1394527754047914 1109 36-37 0.7883891444984812 1109 38-39 1.6404169507436919 1109 40-41 1.3593484169002394 1109 42-43 1.104323731266284 1109 44-45 1.0212004983523926 1109 46-47 1.010283355815183 1109 48-49 0.8423562315024498 1109 50-51 -0.1377926148731703 1109 52-53 0.22909126042042516 1109 54-55 -0.4883233580069586 1109 56-57 0.15081290158966 1109 58-59 -0.46346296417221566 1109 60-61 0.666692853829808 1109 62-63 0.5287926688022537 1109 64-65 1.063473247766865 1109 66-67 0.266642139637554 1109 68-69 -0.7760794286647297 1109 70-71 -0.09938897893588816 1109 72-73 0.2466591531588911 1109 74-75 -0.34887784945721023 1109 76 1.5172722222085575 1110 1 -1.187194453048665 1110 2 -0.6337030715264973 1110 3 0.05196779466299262 1110 4 -0.13959277760142186 1110 5 0.33153656628751804 1110 6 0.01929131670852513 1110 7 0.5598393781341784 1110 8 0.830660412219892 1110 9 0.9876932072575357 1110 10-11 -0.7210305537025192 1110 12-13 0.2939227867786727 1110 14-15 0.9398533385338368 1110 16-17 0.535256013623794 1110 18-19 -0.6766440097940958 1110 20-21 0.9198574498946144 1110 22-23 -1.3254479535909454 1110 24-25 -1.9148615862302378 1110 26-27 1.143836432343896 1110 28-29 -0.1494496093220974 1110 30-31 -0.5614867492516922 1110 32-33 0.8164277558180117 1110 34-35 1.6050547410067537 1110 36-37 1.7820418881512268 1110 38-39 1.211301471628218 1110 40-41 -1.254779347227533 1110 42-43 -0.5712290942865437 1110 44-45 0.8155903427422331 1110 46-47 0.7105700061018325 1110 48-49 1.4885478776940957 1110 50-51 -0.40065092773147626 1110 52-53 0.6181166494458168 1110 54-55 -0.30269682238042606 1110 56-57 0.46964992042667575 1110 58-59 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-0.00913301284393242 2116 50-51 -1.6468475882138591 2116 52-53 -0.41064030788257355 2116 54-55 -0.9174463585585286 2116 56-57 -0.1559530480334388 2116 58-59 -2.532915069338607 2116 60-61 -2.0551769751828743 2116 62-63 0.41834037977853455 2116 64-65 -1.8417023359801448 2116 66-67 -1.0008243064003253 2116 68-69 -2.215820189834062 2116 70-71 0.24819329721782069 2116 72-73 -0.5473746765892251 2116 74-75 -0.2702328779550953 2116 76 -1.2550204786555703 2117 1 0.9360378701836609 2117 2 0.44811511029168827 2117 3 0.16105870375390197 2117 4 0.17454863653998842 2117 5 0.1830517178026696 2117 6 0.1425236399408476 2117 7 0.8396373579321548 2117 8 0.14985233141181453 2117 9 -1.776953257388925 2117 10-11 -0.8215356042075683 2117 12-13 -0.8616327687768859 2117 14-15 -0.1313587826782765 2117 16-17 -1.0329258045580332 2117 18-19 -1.320078353228439 2117 20-21 -0.02761729758013587 2117 22-23 0.032127803984806746 2117 24-25 -1.9259726973413436 2117 26-27 -2.210203971696508 2117 28-29 -0.7024799123523948 2117 30-31 -0.40542614319109305 2117 32-33 -1.9850873956971355 2117 34-35 0.22626686221887837 2117 36-37 -1.776038919929583 2117 38-39 -0.853850043523309 2117 40-41 -0.6067995492477323 2117 42-43 0.17271029965284868 2117 44-45 -0.09905612190423341 2117 46-47 -1.0717532262214036 2117 48-49 -1.1276137384675167 2117 50-51 -0.3900448671254253 2117 52-53 0.7090257403549032 2117 54-55 -0.7355251052087084 2117 56-57 -0.44651169573494087 2117 58-59 -1.428245928955178 2117 60-61 -1.2563210691841178 2117 62-63 -0.9695693295597465 2117 64-65 -2.54995836566475 2117 66-67 -1.2566993837039675 2117 68-69 -0.17711682970212905 2117 70-71 -0.598023977570886 2117 72-73 0.679228085727825 2117 74-75 0.08778608720672665 2117 76 0.6251073300436651 2118 1 0.3383790388799639 2118 2 0.06918562855846488 2118 3 -0.9893236241985193 2118 4 0.1325397627554139 2118 5 0.3365493105525985 2118 6 -0.05666450317252725 2118 7 0.65593113684276 2118 8 -0.00978138896957148 2118 9 -2.2112022897687993 2118 10-11 -1.4821605452063409 2118 12-13 -1.0261281902816535 2118 14-15 0.4240079689501499 2118 16-17 -2.9760014205308423 2118 18-19 -0.731104502572343 2118 20-21 -0.5490465433084424 2118 22-23 0.8204314006996185 2118 24-25 0.9973729082472502 2118 26-27 -2.1090522677410632 2118 28-29 -0.6131284538420587 2118 30-31 -1.2085980491667385 2118 32-33 -0.13561132659489772 2118 34-35 -0.14685689875535246 2118 36-37 -3.2143472367425687 2118 38-39 -0.2503382904788367 2118 40-41 0.3853650877767194 2118 42-43 0.5599943551612014 2118 44-45 -1.9925235060258188 2118 46-47 0.5441723850313167 2118 48-49 -0.28078092434498103 2118 50-51 -0.7467426864400579 2118 52-53 -1.4328604108770406 2118 54-55 -0.6642516227202648 2118 56-57 0.5693950351250621 2118 58-59 -1.1846888629682084 2118 60-61 -0.7114329355109419 2118 62-63 -1.3351881344308936 2118 64-65 0.14375446356210375 2118 66-67 0.2376364984823809 2118 68-69 -0.28138379845507444 2118 70-71 0.5194120650988019 2118 72-73 2.0638688862564756 2118 74-75 1.4680371964297976 2118 76 1.0832400592044316 2119 1 0.867351001496786 2119 2 -0.5700667078901347 2119 3 -0.15308271038750831 2119 4 -0.17898671699536095 2119 5 -0.8472513125003616 2119 6 -0.904951107533897 2119 7 -1.4452111269163268 2119 8 -0.17135978980030586 2119 9 -0.6072562876919605 2119 10-11 -0.15739419006615663 2119 12-13 -0.20759236473648457 2119 14-15 -0.5783284796479791 2119 16-17 -0.3632288348610615 2119 18-19 -0.6453308784809622 2119 20-21 -1.9503445703074007 2119 22-23 -0.311811589954587 2119 24-25 1.020996999628359 2119 26-27 0.3302000687075335 2119 28-29 -0.5449041547766456 2119 30-31 0.8243718366068862 2119 32-33 0.2780439174341751 2119 34-35 0.912125448077461 2119 36-37 0.35325400936334717 2119 38-39 -2.0402136798869392 2119 40-41 -1.8921530846012686 2119 42-43 0.037356764299310896 2119 44-45 -5.71273419375018E-4 2119 46-47 -0.30458150904968306 2119 48-49 -1.1387248495786295 2119 50-51 0.787732910652359 2119 52-53 0.0029651342942926817 2119 54-55 0.11497994529634781 2119 56-57 -1.360148059371305 2119 58-59 -0.2540035047127489 2119 60-61 -0.140664503527546 2119 62-63 -1.0402764002668192 2119 64-65 -0.3757159414223281 2119 66-67 1.0508763738717875 2119 68-69 0.871368018782718 2119 70-71 -0.12176135130825827 2119 72-73 0.4640765705763066 2119 74-75 0.6014224508430885 2119 76 -0.26681186187552797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 6.0 23 12.0 24 39.0 25 90.0 26 156.0 27 263.0 28 514.0 29 768.0 30 1180.0 31 1686.0 32 2508.0 33 3648.0 34 4984.0 35 5321.0 36 6173.0 37 11270.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.090784328957252 25.54442113985344 11.810248841243947 36.554545689945364 2 12.879670628447137 15.427639244931251 53.26911623812113 18.42357388850048 3 12.379916621352184 28.985732411507286 40.205598280639066 18.42875268650146 4 8.705559439654056 26.44035319402367 35.05269426966001 29.801393096662267 5 8.20062663455812 42.93223542815712 38.00201973122038 10.865118206064373 6 17.475853854320412 41.565032755897356 15.476837825940597 25.482275563841633 7 17.48880084932287 39.21903726145162 18.001501851420283 25.290660037805225 8 15.440586239933712 38.7710712343665 24.21605945260105 21.572283073098735 9 15.577824386959788 19.60951863072581 16.517776224138377 48.29488075817603 10-11 16.19280664957663 25.26994484580129 32.728708666718454 25.80853983790362 12-13 21.19811491752764 16.963152852222997 28.700898521453173 33.13783370879619 14-15 23.480670136461328 22.394417255754938 18.252673554468007 35.872239053315724 16-17 25.332090421813096 27.011315673632154 17.236334446774904 30.420259457779846 18-19 22.529066003780525 25.668712291877053 18.393795799994823 33.4084259043476 20-21 20.89127113596934 21.84546466765064 17.487506149822625 39.7757580465574 22-23 23.571299101478544 31.09868199590875 18.228074263963336 27.101944638649368 24-25 25.345037416815558 33.61816722338745 18.162044589450787 22.874750770346203 26-27 27.757062585773838 35.03068437815583 17.995028353919054 19.217224682151272 28-29 24.398612082135738 28.994795308009014 24.194049561096868 22.412543048758383 30-31 36.19202982987649 20.08337864781584 27.32463295269168 16.39995856961599 32-33 31.787462130039618 18.654030399544265 33.9094746109428 15.649032859473316 34-35 34.28234806701364 24.152619177088997 26.34454543100546 15.220487324891893 36-37 25.323027525311375 26.867604029104847 26.471425982029572 21.33794246355421 38-39 20.667288122426786 21.41174033506823 28.366866050389707 29.55410549211528 40-41 18.309640332478832 22.821668090836116 32.3752557031513 26.493435873533755 42-43 26.661746808565734 21.054403273000336 34.12568942748388 18.158160490950053 44-45 18.162044589450787 24.925554778735854 29.948988839690312 26.963411792123047 46-47 27.183510707164864 18.036458737926928 26.849478236101397 27.930552318806807 48-49 23.316243299930086 17.85779020689298 27.992697894818612 30.83326859835832 50-51 15.901499262021284 17.899220590900853 35.20676351018929 30.992516636888578 52-53 18.638494005541315 18.040342836427666 41.45886739687719 21.862295761153835 54-55 16.534607317641576 18.206064372459153 36.22828141588337 29.0310468940159 56-57 25.91341049742355 17.938061575908232 30.411196561278125 25.73733136539009 58-59 17.578135114839846 29.01809989901344 34.50503638105596 18.89872860509076 60-61 20.418705818379554 40.81669644475517 21.756130402133664 17.00846733473161 62-63 13.798907273621792 50.321085476061015 18.511613454517207 17.368393795799992 64-65 13.34705714803594 51.45394753877625 17.962660866412904 17.236334446774904 66-67 13.655195629094488 50.32238017556126 17.72832025686838 18.29410393847588 68-69 13.70309951060359 47.55172324503483 18.291514539475386 20.453662704886195 70-71 14.223568709702478 43.63007845878972 19.35964162717833 22.786711204329475 72-73 14.96931562184417 39.05719982392087 20.06784225381289 25.905642300422073 74-75 15.010746005852042 38.71798855485642 20.417411118879308 25.853854320412236 76 14.889044252828917 39.27600403946244 20.218027395841425 25.616924311867216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 0.5 14 0.5 15 1.5 16 1.0 17 0.0 18 1.5 19 4.0 20 5.5 21 6.0 22 11.0 23 30.0 24 35.5 25 27.0 26 46.5 27 70.0 28 108.0 29 142.0 30 172.5 31 236.0 32 333.0 33 397.0 34 456.5 35 589.0 36 697.5 37 733.0 38 841.0 39 1180.0 40 1716.5 41 2797.5 42 3573.0 43 4278.5 44 4994.5 45 4629.0 46 4253.0 47 3638.0 48 2681.5 49 2049.0 50 1758.0 51 1586.5 52 1245.5 53 1001.0 54 926.0 55 879.0 56 707.5 57 493.0 58 403.0 59 351.5 60 240.0 61 164.5 62 149.0 63 126.0 64 85.5 65 59.5 66 39.5 67 28.0 68 20.5 69 8.0 70 1.5 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 38619.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.562443356896864 #Duplication Level Percentage of deduplicated Percentage of total 1 65.79652358108528 27.346642844195863 2 16.921064108155253 14.065615370672466 3 7.582082113263971 9.45389575079624 4 3.6010217431935705 5.986690489137471 5 2.230390629867298 4.635024210880655 6 1.021743193570494 2.547968616484114 7 0.7600772537536602 2.211346746420156 8 0.4610304653915644 1.5329242082912555 9 0.4298797582705127 1.6080167793055231 >10 1.077814466388387 6.867086149304747 >50 0.031150707121051648 0.9710246251844946 >100 0.06853155566631364 5.173619202982987 >500 0.006230141424210329 2.475465444470338 >1k 0.012460282848420658 15.124679561873688 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT 2976 7.70605142546415 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT 2865 7.41862813640954 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 956 2.475465444470338 No Hit CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 464 1.2014811362282813 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 291 0.7535151091431679 No Hit TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT 256 0.6628861441259484 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT 167 0.43242963308216165 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 159 0.41171444107822575 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT 117 0.3029596830575623 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 115 0.2977808850565784 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT 112 0.29001268805510244 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGTCTT 108 0.2796550920531345 No Hit CTTATAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 105 0.27188689505165853 No Hit CTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 104 0.26929749605116654 No Hit TCTTAATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 93 0.24081410704575468 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGTCTT 86 0.2226883140423108 No Hit TTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 71 0.18384732903493098 No Hit CTTAACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 66 0.17090033403247107 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGTCTT 59 0.15277454102902716 No Hit CTTATTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 50 0.1294699500245993 No Hit CTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 49 0.1268805510241073 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTGGGATCTCGTATGCCGT 46 0.11911235402263134 No Hit CTTATACACATCTCCGAGCCCACGCGACAGGTTGGGATCTCGTATGCCGT 39 0.10098656101918745 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010357596001967943 0.0 2 0.0 0.0 0.0 0.010357596001967943 0.0 3 0.0 0.0 0.0 0.010357596001967943 0.0 4 0.0 0.0 0.0 0.01294699500245993 0.0 5 0.0 0.0 0.0 0.01294699500245993 0.0 6 0.0 0.0 0.0 0.01294699500245993 0.0 7 0.0 0.0 0.0 0.01294699500245993 0.0 8 0.0 0.0 0.0 0.01294699500245993 0.0 9 0.0 0.0 0.0 0.01294699500245993 0.0 10 0.0 0.0 0.0 0.01294699500245993 0.0 11 0.0 0.0 0.0 0.01294699500245993 0.0 12 0.0 0.0 0.0 0.01294699500245993 0.0 13 0.0 0.0 0.0 0.01294699500245993 0.0 14 0.0 0.0 0.0 0.01294699500245993 0.0 15 0.0 0.0 0.0 0.015536394002951915 0.0 16 0.0 0.0 0.0 0.015536394002951915 0.0 17 0.0 0.0 0.0 0.0181257930034439 0.0 18 0.0 0.0 0.0 0.020715192003935887 0.0 19 0.0 0.0 0.0 0.028483389005411842 0.0 20 0.0 0.0 0.0 0.03366218700639582 0.0 21 0.0 0.0 0.0 0.038840985007379786 0.0 22 0.0 0.0 0.0 0.04143038400787177 0.0 23 0.0 0.0 0.0 0.04143038400787177 0.0 24 0.0 0.0 0.0 0.05178798000983972 0.0 25 0.0 0.0 0.0 0.054377379010331704 0.0 26 0.0 0.0 0.0 0.056966778010823685 0.0 27 0.0 0.0 0.0 0.08027136901525156 0.0 28 0.0 0.0 0.0 0.10357596001967943 0.0 29 0.0 0.0 0.0 0.13464874802558327 0.0 30 0.0 0.0 0.0 0.15795333903001113 0.0 31 0.0 0.0 0.0 0.17348973303296306 0.0 32 0.0 0.0 0.0 0.2226883140423108 0.0 33 0.0 0.0 0.0 0.27188689505165853 0.0 34 0.0 0.0 0.0 0.35992646106838605 0.0 35 0.0 0.0 0.0 0.4013568450762578 0.0 36 0.0 0.0 0.0 0.5204691990988891 0.0 37 0.0 0.0 0.0 0.7017271291333281 0.0 38 0.0 0.0 0.0 0.9943292161889226 0.0 39 0.0 0.0 0.0 1.3128252932494369 0.0 40 0.0 0.0 0.0 1.6598047593153629 0.0 41 0.0 0.0 0.0 2.011963023382273 0.0 42 0.0 0.0 0.0 2.4884124394727984 0.0 43 0.0 0.0 0.0 2.8328025065382323 0.0 44 0.0 0.0 0.0 3.2212123566120305 0.0 45 0.0 0.0 0.0 3.669178383697144 0.0 46 0.0 0.0 0.0 4.251793158807841 0.0 47 0.0 0.0 0.0 4.666096998886559 0.0 48 0.0 0.0 0.0 5.0726326419638 0.0 49 0.0 0.0 0.0 5.461042492037598 0.0 50 0.0 0.0 0.0 5.823558352106476 0.0 51 0.0 0.0 0.0 6.20678940417929 0.0 52 0.0 0.0 0.0 6.61073564825604 0.0 53 0.0 0.0 0.0 7.012092493332298 0.0 54 0.0 0.0 0.0 7.377197752401668 0.0 55 0.0 0.0 0.0 7.708640824464641 0.0 56 0.0 0.0 0.0 8.097050674538439 0.0 57 0.0 0.0 0.0 8.451798337605842 0.0 58 0.0 0.0 0.0 8.837618788679148 0.0 59 0.0 0.0 0.0 9.148346668738187 0.0 60 0.0 0.0 0.0 9.510862528807063 0.0 61 0.0 0.0 0.0 9.875967787876434 0.0 62 0.0 0.0 0.0 10.18410626893498 0.0 63 0.0 0.0 0.0 10.572516119008778 0.0 64 0.0 0.0 0.0 10.971283565084544 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTA 15 0.0022086063 70.00001 8 ATTACAC 20 7.8321056E-5 69.99999 4 CTTATAA 20 7.8321056E-5 69.99999 1 TTTACAC 170 0.0 63.82353 2 CTTTACA 175 0.0 60.0 1 CTATACA 90 0.0 58.33333 1 TATTACA 25 2.3621052E-4 56.000004 3 TTTATAC 20 0.006901503 52.499996 3 TTACACA 210 0.0 51.666664 3 CTTAATA 30 5.808687E-4 46.666668 2 TTAATAC 35 0.0012407555 40.0 3 GAACAAA 55 2.940566E-4 31.818184 60 CACGAGC 45 0.0042575714 31.111109 21 ACGAGCC 45 0.0042575714 31.111109 22 TAATACA 45 0.0042575714 31.111109 4 ATATCGT 45 0.0042575714 31.111109 34 TTGGGAC 70 3.4051493E-5 30.0 32 TGAACAA 60 4.896664E-4 29.166668 59 TGGGACC 60 4.896664E-4 29.166668 33 GGACCTC 50 0.007125715 28.000002 32 >>END_MODULE