FastQCFastQC Report
Wed 25 May 2016
SRR1780361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292752
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT301.2941234E-436.6887735
ACCAGAA250.002348854735.20314824
GTTTACA602.9433795E-729.3209081
CGGGACA300.00575949629.31088632
TGCTCCC300.00575949629.31088633
TACGGTG407.0437446E-427.48365428
TTAATAC407.0790184E-427.4601823
TATAGAT407.0790184E-427.4601825
GCGTGTA1300.027.09324834
CCGTGTA756.933806E-826.41591834
GGATCTC450.001390788624.45918340
GTCTACT450.001399132424.4340921
CAAGTGA450.00140052724.42991428
CACGTCT450.00140611724.4132217
ACACGTC450.001407517424.4090526
ACCTTTG551.5978704E-423.98163442
TTCATAC551.6052973E-423.9652523
CGGTGGT1500.023.4487143
CAGAGGG752.0814423E-623.432693
CTATACA1053.0613592E-923.0378571
Produced by FastQC (version 0.11.5)