FastQCFastQC Report
Wed 25 May 2016
SRR1780361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292752
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT277679.484819915833196No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT233577.978425424933048Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC72132.463860195660491No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC37341.27548231950593RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG33421.1415805869814724No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT21410.731335738099142No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC17830.6090479313548669No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC13570.4635322730502268No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG9730.33236322894463577No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC9010.3077690331748374No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG6980.23842706454610044No Hit
CTTAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC6450.22032300377111003No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC4750.1622533748701973RNA PCR Primer, Index 47 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG4740.161911788817839No Hit
CTTATTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG3490.11921353227305022No Hit
CTTATCACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3460.11818877411597528RNA PCR Primer, Index 47 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA650.044.04361
AGGCGTC207.848207E-443.99846627
CCGTCTT24450.042.46886444
TTTACAC7700.039.433932
GCCGTCT27650.037.5538443
TCTTTAT1900.037.0893441
CTTTACA8550.035.8015171
CTATACA5600.035.3921781
GCAACAA250.002338498635.2348751
CGAATGT250.00234832535.204784
CAGAATT250.00235029435.1987731
TGTCTTC250.00235029435.1987718
TTACACA9350.032.4750023
CTTTATA2250.032.2710532
CTTATAC39650.031.5469911
ATTAGAG353.209395E-431.432843
GATGCTG353.2126124E-431.42747335
TTATACA40450.030.5702842
CTATAAC602.9290095E-729.3373174
TCGAATG300.00573418629.3373173
Produced by FastQC (version 0.11.5)