FastQCFastQC Report
Wed 25 May 2016
SRR1780360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49554
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGT150.00221321369.9929354
ACTTATT150.00221321369.99293541
TACGGAC150.00221321369.99293538
GATAAAG200.00691579852.494737
TTTACGG200.00691579852.494736
GTTTAGC200.00691579852.494711
TTATGAC200.00691579852.494750
ATATAGG200.00691579852.49474
TTACGGA200.00691579852.494737
CGATGTG200.00691579852.494718
TCGGTGG200.00691579852.494742
TGGTCGC200.00691579852.494746
GACTCCA200.00691579852.494742
GTATAAG305.627199E-446.99391
GAGACAG305.8277056E-446.6619577
TATAGGC305.8277056E-446.6619575
AGATACC350.00124479539.99596427
TAAGAGA350.00124479539.9959644
CGCCCGA350.00124479539.99596414
TGTAGAT450.0042712831.10797334
Produced by FastQC (version 0.11.5)