##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780360_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49554 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.154659563304676 34.0 23.0 34.0 12.0 34.0 2 28.936776042297293 34.0 23.0 34.0 12.0 34.0 3 29.216269120555353 34.0 27.0 34.0 11.0 34.0 4 29.480082334423052 34.0 31.0 34.0 11.0 34.0 5 29.520886305848165 34.0 31.0 34.0 11.0 34.0 6 31.656213423739757 37.0 27.0 38.0 11.0 38.0 7 31.019312265407436 36.0 27.0 38.0 11.0 38.0 8 30.548351293538364 36.0 26.0 38.0 11.0 38.0 9 31.02314646648101 36.0 26.0 38.0 12.0 38.0 10-11 31.249041449731607 37.0 27.0 38.0 11.0 38.0 12-13 31.548240303507285 37.0 27.5 38.0 11.0 38.0 14-15 31.207309198046573 37.0 27.0 38.0 11.0 38.0 16-17 31.0176171449328 37.0 26.5 38.0 11.0 38.0 18-19 31.02947289825241 37.0 26.5 38.0 11.0 38.0 20-21 31.106530249828467 37.0 26.5 38.0 11.0 38.0 22-23 30.982978165233888 37.0 26.0 38.0 11.0 38.0 24-25 31.351576058441296 37.0 27.0 38.0 11.0 38.0 26-27 31.37612503531501 37.0 27.0 38.0 11.0 38.0 28-29 31.40152157242604 37.0 27.0 38.0 11.0 38.0 30-31 31.231696734875086 37.0 27.0 38.0 11.0 38.0 32-33 31.108417080356784 37.0 26.5 38.0 11.0 38.0 34-35 31.049945513984746 37.0 26.0 38.0 11.0 38.0 36-37 31.006639221858983 37.0 26.0 38.0 11.0 38.0 38-39 31.35611655971264 37.0 27.0 38.0 11.0 38.0 40-41 30.99099971747992 37.0 26.0 38.0 11.0 38.0 42-43 31.28708076038261 37.0 27.0 38.0 11.0 38.0 44-45 30.427008919562496 36.0 25.5 38.0 11.0 38.0 46-47 31.01934253541591 37.0 26.0 38.0 11.0 38.0 48-49 30.866922952738427 37.0 26.0 38.0 11.0 38.0 50-51 30.845542236751825 36.5 26.0 38.0 11.0 38.0 52-53 30.83367639342939 37.0 26.0 38.0 11.0 38.0 54-55 30.612442991484038 36.0 26.0 38.0 11.0 38.0 56-57 30.6030592888566 36.5 25.5 38.0 11.0 38.0 58-59 30.51206764337894 36.5 25.5 38.0 11.0 38.0 60-61 30.52926100819308 36.5 25.0 38.0 11.0 38.0 62-63 30.54985470395932 36.0 25.0 38.0 11.0 38.0 64-65 30.643368850143275 36.5 25.0 38.0 11.0 38.0 66-67 30.625670985187874 36.5 25.0 38.0 11.0 38.0 68-69 30.22411914275336 36.0 24.5 38.0 11.0 38.0 70-71 30.10808411026355 36.0 24.0 38.0 11.0 38.0 72-73 30.21791379101586 36.0 24.0 38.0 11.0 38.0 74-75 29.870847963837427 35.5 24.0 38.0 11.0 38.0 76 29.092787665980545 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.6223372812877273 1101 2 -1.7617219729851215 1101 3 0.012224936877000658 1101 4 -2.5150676529999174 1101 5 -1.5230609637806154 1101 6 -1.8823389188866564 1101 7 -1.091022784279236 1101 8 -2.149833748291801 1101 9 -1.618141298294148 1101 10-11 -1.6644187111372197 1101 12-13 -2.7313619511868303 1101 14-15 -1.925664634368701 1101 16-17 -3.1178923142016046 1101 18-19 -1.6198075444024482 1101 20-21 -1.663484968623905 1101 22-23 -1.3018140310450939 1101 24-25 -2.164227813292893 1101 26-27 -1.8024785511302603 1101 28-29 -1.1981355911800726 1101 30-31 -1.637062814574758 1101 32-33 -1.4765310713207782 1101 34-35 -1.4203759761002672 1101 36-37 -1.3103008003727723 1101 38-39 -0.7693290845088718 1101 40-41 -0.2380044253489686 1101 42-43 -1.0826381296912864 1101 44-45 -0.9434134187212564 1101 46-47 -0.9693132601949088 1101 48-49 -1.9286006241333773 1101 50-51 -1.1544094682223331 1101 52-53 -0.726148754957709 1101 54-55 -2.3031840475244536 1101 56-57 -1.3224879842157193 1101 58-59 -1.9635893331638101 1101 60-61 -0.6897934444330005 1101 62-63 -3.3667190743448074 1101 64-65 -0.910675851192817 1101 66-67 -1.8053079667144623 1101 68-69 -0.5735342293236165 1101 70-71 -1.2453706289167705 1101 72-73 -1.223082706326224 1101 74-75 -3.0351251563713255 1101 76 -2.216514754399647 1102 1 -0.3572331146210601 1102 2 -0.320055306318455 1102 3 -1.4075667297896644 1102 4 -1.3088176529999167 1102 5 -0.3824359637806154 1102 6 -1.5354639188866557 1102 7 0.8891855490540976 1102 8 0.8803745850415332 1102 9 0.4209212017058519 1102 10-11 -0.3485332944705526 1102 12-13 -4.635268201186829 1102 14-15 -1.757695884368701 1102 16-17 -3.4426318975349375 1102 18-19 -1.1166825444024475 1102 20-21 -0.8819745519572386 1102 22-23 -1.715095281045091 1102 24-25 -1.4676132299595572 1102 26-27 -5.037113967796927 1102 28-29 -1.649698091180074 1102 30-31 -1.2500836479080917 1102 32-33 -5.999968571320778 1102 34-35 -0.9669905594336008 1102 36-37 -1.7428528837061066 1102 38-39 -0.8482353345088747 1102 40-41 -1.103889842015633 1102 42-43 -0.7823777130246192 1102 44-45 -4.991850918721255 1102 46-47 -2.540927843528241 1102 48-49 -0.7684443741333773 1102 50-51 -5.452065718222336 1102 52-53 -2.0172945882910405 1102 54-55 -1.7953715475244536 1102 56-57 -3.0784775675490508 1102 58-59 -0.7763497498304766 1102 60-61 0.1448419722336638 1102 62-63 -4.615156574344809 1102 64-65 -1.1382800178594827 1102 66-67 -1.3868183833811294 1102 68-69 -6.408950895990284 1102 70-71 -1.540683128916772 1102 72-73 -1.3668327063262211 1102 74-75 -6.270281406371328 1102 76 -2.9383897543996476 1103 1 -0.3208880059254078 1103 2 -0.6914321179126581 1103 3 -0.9544417297896644 1103 4 0.6381932165653019 1103 5 -0.04298850001249832 1103 6 -0.33596210729245257 1103 7 0.40571634615554686 1103 8 0.8733546575053026 1103 9 0.8057717451841135 1103 10-11 0.6759798395874164 1103 12-13 -1.5842605019114657 1103 14-15 0.18846580041390837 1103 16-17 -0.051044488114648345 1103 18-19 -0.2455321820836076 1103 20-21 -0.4093183019572386 1103 22-23 0.18713524431722917 1103 24-25 -0.23940897271318207 1103 26-27 -1.0267539134490988 1103 28-29 0.32324122041412906 1103 30-31 0.29373428687451764 1103 32-33 -2.842473100306286 1103 34-35 0.05842837172582094 1103 36-37 0.29603977933737013 1103 38-39 0.5632796111432974 1103 40-41 0.55275644421625 1103 42-43 0.45884737755509164 1103 44-45 -3.6776525491560363 1103 46-47 0.10858529052973154 1103 48-49 0.5560008251419859 1103 50-51 -0.44589497546871115 1103 52-53 -0.3527791897403141 1103 54-55 0.7321420394320697 1103 56-57 0.4856641896973244 1103 58-59 0.4335121161115545 1103 60-61 1.2830326425235192 1103 62-63 1.2171577372493942 1103 64-65 0.5273676270680525 1103 66-67 -0.35403985077243405 1103 68-69 -3.514476258309127 1103 70-71 -0.0075083100761936805 1103 72-73 0.5448520762824742 1103 74-75 -0.8936396491249532 1103 76 0.3385577093684695 1104 1 -1.1680793737451474 1104 2 0.3177184163092832 1104 3 0.14044878115923964 1104 4 -0.12838882088312786 1104 5 0.39026020410259576 1104 6 -0.1151856342151234 1104 7 1.8815441256964363 1104 8 2.225835351464891 1104 9 0.46032585499052203 1104 10-11 0.433543575967402 1104 12-13 -0.1295371336685811 1104 14-15 0.14276602256560622 1104 16-17 1.0430261772825808 1104 18-19 0.8471632585172593 1104 20-21 1.6527254936632012 1104 22-23 1.6331312244293592 1104 24-25 0.8739412864638005 1104 26-27 0.6901613515461413 1104 28-29 0.9144556496958351 1104 30-31 0.11847246523059596 1104 32-33 -0.6225164727806316 1104 34-35 0.35751331830362787 1104 36-37 0.22780747213331054 1104 38-39 0.4522037622064552 1104 40-41 1.4570989809770651 1104 42-43 1.2662365661724628 1104 44-45 -0.9241615939037366 1104 46-47 0.8387767641359893 1104 48-49 1.2187533904651637 1104 50-51 1.2710467361572242 1104 52-53 1.4344047730228304 1104 54-55 0.502929091161679 1104 56-57 0.6701823320859859 1104 58-59 1.3255834162279179 1104 60-61 1.2861797915767283 1104 62-63 0.8211458891588421 1104 64-65 0.9367085770310304 1104 66-67 0.6834085782977048 1104 68-69 -2.3389235237275123 1104 70-71 0.21165491852848461 1104 72-73 1.1516435710460442 1104 74-75 0.23613396771626327 1104 76 1.1440691872061919 1105 1 -0.5039464766900252 1105 2 -0.12436565114604292 1105 3 -1.0234072470310451 1105 4 0.12200131251732316 1105 5 0.22262869139179742 1105 6 -0.3202699533694151 1105 7 0.3759312387092706 1105 8 0.8317754471104983 1105 9 0.4422035292920583 1105 10-11 -0.4033554927464138 1105 12-13 -1.381361951186829 1105 14-15 -0.026526703334219803 1105 16-17 -0.6938980613280421 1105 18-19 -0.45860064785072296 1105 20-21 -0.6058700260951717 1105 22-23 -0.710865755183022 1105 24-25 -0.9580496523733508 1105 26-27 -0.451041769521062 1105 28-29 -1.49037697049042 1105 30-31 -0.8953961479080945 1105 32-33 -0.7765310713207789 1105 34-35 -0.6886231025370471 1105 36-37 -0.049237581981969925 1105 38-39 -0.8262256362330156 1105 40-41 -0.6973435058087389 1105 42-43 0.04308026111331387 1105 44-45 -0.002465142859186642 1105 46-47 -0.5552328004247933 1105 48-49 -1.2652385551678584 1105 50-51 -1.2496680889119887 1105 52-53 -1.1487062262220746 1105 54-55 -1.3243047371796237 1105 56-57 -1.0347006278938764 1105 58-59 -0.39060082741668367 1105 60-61 -0.3205405708697846 1105 62-63 -0.5154259708965334 1105 64-65 -0.8316528626870685 1105 66-67 -0.7568884264845757 1105 68-69 -0.8341664132316602 1105 70-71 0.35678454349702093 1105 72-73 -0.8855827063262183 1105 74-75 -1.8829699839575333 1105 76 -0.8647906164686141 1106 1 0.5669100305402317 1106 2 0.7081705001331571 1106 3 0.44878407666194775 1106 4 0.6921904115162114 1106 5 0.029358391058092792 1106 6 0.8612094682101201 1106 7 0.8060202264734535 1106 8 1.035112488267341 1106 9 1.5592780565445636 1106 10-11 0.8010382781100915 1106 12-13 1.5057348230067227 1106 14-15 0.6630450430506514 1106 16-17 0.9443388685940945 1106 18-19 0.3576118104362642 1106 20-21 0.3245526657846973 1106 22-23 0.2993956463742613 1106 24-25 1.1106377781049552 1106 26-27 1.0864999434933935 1106 28-29 -0.3949097847284584 1106 30-31 0.699765142414492 1106 32-33 1.5154044125501898 1106 34-35 1.8257261744373672 1106 36-37 1.8454787695197012 1106 38-39 1.1355096251685488 1106 40-41 1.9683127789521109 1106 42-43 2.1650769240721566 1106 44-45 1.8037640006335849 1106 46-47 1.6409017935685277 1106 48-49 1.3959961500601743 1106 50-51 1.228251822100244 1106 52-53 0.3957598471928314 1106 54-55 1.4730256298948987 1106 56-57 2.073614166321917 1106 58-59 0.8850665808146836 1106 60-61 1.5050990286852794 1106 62-63 1.6522325385584153 1106 64-65 1.3589478047211614 1106 66-67 1.7252027859737034 1106 68-69 1.1606055556226202 1106 70-71 0.8058390485025804 1106 72-73 0.5394172936737789 1106 74-75 -0.05568967250036039 1106 76 1.1125175036648685 1107 1 -0.734837281287728 1107 2 -0.6603330840962336 1107 3 0.13583604798811422 1107 4 -0.478956541888806 1107 5 -0.12028318600283683 1107 6 0.5079388588911229 1107 7 0.23119943794298692 1107 8 -0.2151115260695775 1107 9 -1.0139746316274802 1107 10-11 -0.3915020444705526 1107 12-13 -0.18691750674238605 1107 14-15 -0.6339979677020331 1107 16-17 -0.2512256475349375 1107 18-19 0.8399146778197739 1107 20-21 1.14970947582054 1107 22-23 0.01624152451046612 1107 24-25 -0.12950559107066795 1107 26-27 -0.3691452177969268 1107 28-29 -0.5932744800689598 1107 30-31 -0.4453961479080917 1107 32-33 0.7387467064570004 1107 34-35 0.8268462461219563 1107 36-37 0.11956031073833628 1107 38-39 0.3501153599355682 1107 40-41 -0.29703220312674716 1107 42-43 0.10694520364204863 1107 44-45 0.48991991461208073 1107 46-47 0.19040896202731616 1107 48-49 0.644316042533287 1107 50-51 0.9442016428887747 1107 52-53 1.0703790228200738 1107 54-55 0.5301492858088821 1107 56-57 0.9219564602287278 1107 58-59 0.8759940001695234 1107 60-61 -0.7279878888774469 1107 62-63 -0.7167190743448089 1107 64-65 0.5205741488071851 1107 66-67 0.47733092217442774 1107 68-69 0.5077157706763806 1107 70-71 -0.1787039622501041 1107 72-73 1.1192784047848896 1107 74-75 0.3864026214064502 1107 76 0.35848524560035244 1108 1 0.2936251600268278 1108 2 0.17466300354070086 1108 3 -0.3840191945783964 1108 4 0.005002769535295215 1108 5 0.8977136841067086 1108 6 0.10780192618376816 1108 7 1.0975922391949418 1108 8 0.6046263456049132 1108 9 -0.6780004532237243 1108 10-11 -0.4095478191184405 1108 12-13 0.034131006559650956 1108 14-15 0.11834945013833931 1108 16-17 0.004936324296046735 1108 18-19 0.3227980894003686 1108 20-21 0.5378647966343131 1108 22-23 0.0469535745887093 1108 24-25 -0.07121138136800553 1108 26-27 -0.19132831638847847 1108 28-29 -0.197783478504018 1108 30-31 -0.2939876972038675 1108 32-33 0.9988210413552778 1108 34-35 0.2387202680311873 1108 36-37 -0.2821904717342747 1108 38-39 0.3229244366178854 1108 40-41 0.5205049643223951 1108 42-43 -0.1393869559823635 1108 44-45 -0.9904204609747751 1108 46-47 -0.402998706204297 1108 48-49 -0.17419921568267327 1108 50-51 -0.2901489048420558 1108 52-53 -0.5138834967417445 1108 54-55 -0.55230376583431 1108 56-57 0.9496364289298214 1108 58-59 -0.7908193801121648 1108 60-61 0.862436602515352 1108 62-63 -0.6242894968800208 1108 64-65 -0.9079763206763865 1108 66-67 0.15989156380197045 1108 68-69 0.8960667096435202 1108 70-71 1.262904018970552 1108 72-73 0.7031496880399786 1108 74-75 0.6513185056005035 1108 76 0.27925989348767644 1109 1 -0.5913349502853968 1109 2 -0.7422581035212517 1109 3 -0.0033927787407144194 1109 4 -0.8855221984544634 1109 5 0.12641456069490786 1109 6 -0.00891234546008235 1109 7 -1.3770950453514956 1109 8 0.4995835011254499 1109 9 0.5024880723352219 1109 10-11 0.6589787247602175 1109 12-13 -0.12262069244556884 1109 14-15 -0.11360169730576075 1109 16-17 0.029805820996529064 1109 18-19 0.3349127353178325 1109 20-21 0.4613110686721349 1109 22-23 0.7185531018220424 1109 24-25 0.1826544710893927 1109 26-27 0.4236869500352398 1109 28-29 0.5751161570716725 1109 30-31 0.39201643950449494 1109 32-33 0.9115808167911084 1109 34-35 1.1327417395174528 1109 36-37 1.1019078243358535 1109 38-39 0.6023492371694452 1109 40-41 1.0669198170752736 1109 42-43 1.1751998656467073 1109 44-45 0.24443623162839856 1109 46-47 0.33808767220602576 1109 48-49 0.4651930821603294 1109 50-51 0.6442793429664775 1109 52-53 0.35196896066000605 1109 54-55 0.0958544140140063 1109 56-57 0.4933919691642359 1109 58-59 -0.10892732850180309 1109 60-61 -0.1865883162278763 1109 62-63 0.3379137927880578 1109 64-65 0.3392658737489107 1109 66-67 1.2497911008846039 1109 68-69 1.3159179851285963 1109 70-71 -0.1412622373083785 1109 72-73 0.5717599510164355 1109 74-75 1.6455566618104918 1109 76 -1.0514797893646808 1110 1 0.13069302174257658 1110 2 -0.20263106389421282 1110 3 -1.0453508206987543 1110 4 -0.16582522875749106 1110 5 -0.20109126681091993 1110 6 0.28129744474970764 1110 7 0.058219639963187575 1110 8 1.4092571607991076 1110 9 -0.8302625104153591 1110 10-11 -1.1254035596220682 1110 12-13 -1.09348316330804 1110 14-15 -0.387785846489912 1110 16-17 0.1180925342832424 1110 18-19 0.13852578893088463 1110 20-21 -0.16310618074511396 1110 22-23 0.08947384774278788 1110 24-25 0.7808479442828649 1110 26-27 -0.6873270359787469 1110 28-29 0.288985620941137 1110 30-31 0.05081597330402943 1110 32-33 1.1371052923155887 1110 34-35 0.6103058420815515 1110 36-37 -0.19704322461520007 1110 38-39 0.4927921276123364 1110 40-41 0.5138895140449726 1110 42-43 0.6306194460662873 1110 44-45 1.181207793399956 1110 46-47 1.0799291640475168 1110 48-49 1.1414751334423805 1110 50-51 0.6626359863231173 1110 52-53 0.0795330632241118 1110 54-55 0.15704322520281977 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-0.019622646516907594 2116 52-53 0.15573109000353114 2116 54-55 -0.0749863731058511 2116 56-57 0.49737635686955173 2116 58-59 -0.5360699533188473 2116 60-61 -0.36905701032447524 2116 62-63 0.7420987551125577 2116 64-65 0.4864171720629962 2116 66-67 0.41087420382817186 2116 68-69 1.0937138326918863 2116 70-71 -2.3429481482966175 2116 72-73 0.9299211696427712 2116 74-75 -1.5175863966814056 2116 76 -1.4506232815314313 2117 1 -0.8556706146210615 2117 2 -0.19505530631845502 2117 3 0.38722493687700066 2117 4 0.45159901366675115 2117 5 -0.039727630447281115 2117 6 -2.399005585553322 2117 7 -3.174356117612568 2117 8 -1.5831670816251346 2117 9 -0.21814129829414952 2117 10-11 0.30224795552944883 2117 12-13 0.44363804881317037 2117 14-15 -0.6381646343686995 2117 16-17 -0.22205898086826892 2117 18-19 -0.7531408777357811 2117 20-21 -0.788484968623905 2117 22-23 -0.19764736437842245 2117 24-25 0.06910552004044135 2117 26-27 0.4225214488697375 2117 28-29 0.18519774215326024 2117 30-31 -0.11622948124142596 2117 32-33 0.48596892867922037 2117 34-35 -0.8453759761002679 2117 36-37 -0.922800800372773 2117 38-39 -0.10682908450887751 2117 40-41 -1.4588377586823 2117 42-43 -1.4993047963579507 2117 44-45 -0.2934134187212578 2117 46-47 -0.10681326019490811 2117 48-49 -1.7952672908000444 2117 50-51 -0.01690946822233741 2117 52-53 -0.2636487549577069 2117 54-55 -1.0823507141911222 2117 56-57 -1.1433213175490522 2117 58-59 -0.24692266649714512 2117 60-61 0.3685398889003295 2117 62-63 -0.9375524076781403 2117 64-65 -0.5940091845261506 2117 66-67 -2.042807966714463 2117 68-69 -0.7568675626569501 2117 70-71 -0.3120372955834405 2117 72-73 -0.10641603965955326 2117 74-75 -1.5517918230379948 2117 76 -0.8831814210663147 2118 1 -0.10838379291563527 2118 2 0.4115338409683673 2118 3 0.061062146179327925 2118 4 0.8459788586279906 2118 5 0.22984601296357 2118 6 -0.10947070183239305 2118 7 0.44211675060448385 2118 8 -3.8506089420902505 2118 9 -0.2789940114724416 2118 10-11 -0.6412597964085371 2118 12-13 0.44809541315425605 2118 14-15 -0.8297344018105619 2118 16-17 -0.07826053125586796 2118 18-19 -0.6192261490536097 2118 20-21 -1.6651322554456094 2118 22-23 -0.24764736437842316 2118 24-25 -0.22963479003707832 2118 26-27 -0.20150955888220068 2118 28-29 -0.19639140513356423 2118 30-31 -0.15663645798561276 2118 32-33 -0.48428300930527257 2118 34-35 1.1070465045198823 2118 36-37 0.3609201298597853 2118 38-39 0.14113603177019485 2118 40-41 -0.7530238051939264 2118 42-43 -0.4868047963579514 2118 44-45 0.6832338681004515 2118 46-47 -0.10206519817940318 2118 48-49 0.1542482130759275 2118 50-51 -0.4033435767494673 2118 52-53 -1.406284413872438 2118 54-55 0.2467190532507395 2118 56-57 1.2376864343889338 2118 58-59 0.99106182962689 2118 60-61 -0.9310725142004443 2118 62-63 0.7304708481358091 2118 64-65 -0.21125724654165268 2118 66-67 -2.2751723077997354 2118 68-69 0.5898378636996391 2118 70-71 -0.1879093886066947 2118 72-73 -0.9886834815200203 2118 74-75 0.15295623897750943 2118 76 0.5416247804840744 2119 1 0.8391569715858367 2119 2 1.029082624716029 2119 3 0.7007306840034389 2119 4 0.5220013125173253 2119 5 0.6191804155297298 2119 6 0.8142128052512732 2119 7 -0.6016549681872831 2119 8 1.5921202746967076 2119 9 1.2180655982575743 2119 10-11 1.0750927831156538 2119 12-13 1.239327703985584 2119 14-15 1.5053698483899183 2119 16-17 0.8242053869478241 2119 18-19 0.33967521421824287 2119 20-21 -0.8274217502330998 2119 22-23 1.762410106885941 2119 24-25 0.5591917269369944 2119 26-27 1.6567168511685963 2119 28-29 1.4173816501992427 2119 30-31 1.145121093471218 2119 32-33 0.7217447907481898 2119 34-35 1.5363768974629544 2119 36-37 0.3378313835352742 2119 38-39 0.6513605706635381 2119 40-41 -0.45424005753287133 2119 42-43 -0.9379542216453096 2119 44-45 0.12425899507184823 2119 46-47 0.7878706478510686 2119 48-49 0.22441661724593231 2119 50-51 1.7684353593638704 2119 52-53 -0.007326915877246876 2119 54-55 -0.35706335786927923 2119 56-57 1.9178855790026716 2119 58-59 1.3878474484453882 2119 60-61 1.1492870153371122 2119 62-63 0.6130223049655363 2119 64-65 1.3485195511060333 2119 66-67 0.200870194205077 2119 68-69 -0.2634767580592481 2119 70-71 -0.41218097374435914 2119 72-73 -0.9907551201193243 2119 74-75 -0.23296998395753477 2119 76 0.11279559042793963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 19.0 13 109.0 14 448.0 15 844.0 16 1301.0 17 1522.0 18 1732.0 19 1614.0 20 1456.0 21 1154.0 22 939.0 23 703.0 24 709.0 25 628.0 26 663.0 27 744.0 28 922.0 29 1070.0 30 1257.0 31 1512.0 32 2041.0 33 2522.0 34 3542.0 35 5100.0 36 8201.0 37 8801.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.065908398497 27.982126536000813 9.783690464100742 29.16827460140144 2 16.58392864350002 22.936594422246436 30.572708560358397 29.906768373895144 3 15.58098236267506 24.885982968075233 27.600193728054244 31.93284094119546 4 11.37950518626145 19.76833353513339 32.44137708358558 36.41078419501957 5 11.113129111676152 38.29559672276709 31.18416273156556 19.4071114339912 6 24.942486983896355 36.50966622270654 17.387092868386002 21.1607539250111 7 22.44823828550672 31.03886669088267 19.219437381442468 27.29345764216814 8 19.54635347297897 37.738628566816 17.578802922064817 25.136215038140207 9 20.432255721031602 21.844856116559715 19.080195342454694 38.642692819953986 10-11 20.74504580861283 27.064414578036082 25.810227226863624 26.38031238648747 12-13 20.881260846753037 24.46623885054688 25.004036001130082 29.648464301570005 14-15 20.003430600960566 26.600274448076846 22.418977277313637 30.977317673648948 16-17 20.980142874440006 28.376114945312185 23.82350567058159 26.820236509666223 18-19 19.861161561125236 27.05230657464584 24.655930903660654 28.43060096056827 20-21 19.14477136053598 27.859506800661904 24.835532953949226 28.16018888485289 22-23 21.94373814424668 27.448843685676234 23.85377567905719 26.753642491019896 24-25 20.209266658594665 28.98050611454171 24.068692739233967 26.741534487629657 26-27 19.26383339387335 29.551600274448077 25.195746054808893 25.988820276869678 28-29 20.791459821608747 27.769705775517618 26.57101343988376 24.86782096298987 30-31 20.729910804375027 27.611292731161964 26.468095411066717 25.190701053396296 32-33 18.8844492876458 29.675707309198046 25.87581224522743 25.564031157928724 34-35 19.699721515922025 28.754490051257214 25.838479234774187 25.707309198046573 36-37 20.207248658029624 27.84840779755418 26.264277353997663 25.680066190418533 38-39 19.777414537676073 27.016991564757635 27.549743713928237 25.65585018363805 40-41 19.312265407434314 26.757678492149978 27.17035960770069 26.75969649271502 42-43 18.121645074060623 26.01202728336764 28.265124914234974 27.601202728336766 44-45 17.864350002018 26.92719053961335 28.33575493401138 26.872704524357268 46-47 18.533317189328812 27.02304556645276 27.66174274528797 26.78189449893046 48-49 17.844169996367597 28.242926908019534 27.013964563910076 26.898938531702786 50-51 17.731161964725352 28.061306857165917 26.29051136134318 27.91701981676555 52-53 17.363885861888043 27.515437704322558 26.7788674980829 28.341808935706503 54-55 17.34975985793276 27.815110788231024 26.589175444969126 28.245953908867094 56-57 18.025790047221214 27.2409896274771 26.382330387052512 28.350889938249185 58-59 17.816926988739556 27.164305606005566 26.555878435645962 28.462888969608912 60-61 17.312426847479518 26.2612503531501 26.701174476328855 29.725148323041527 62-63 17.5576139161319 27.17338660854825 26.37123138394479 28.897768091375063 64-65 18.34766113734512 27.620373733704646 24.649876901965534 29.382088226984703 66-67 17.635306937885943 28.57690600153368 24.265447794325382 29.522339266254992 68-69 17.80986398676192 28.275214917060175 24.26040279291278 29.654518303265125 70-71 18.11155507123542 28.434636961698352 24.33305081325423 29.120757153812004 72-73 18.21346409976995 28.72018404165153 23.556120595713768 29.510231262864757 74-75 18.71291923961739 28.643500020180007 23.30387052508375 29.33971021511886 76 18.11155507123542 28.657626024135286 23.70948863865682 29.521330265972473 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 2.0 11 2.0 12 1.0 13 2.0 14 2.0 15 2.5 16 7.5 17 11.0 18 11.0 19 12.5 20 16.5 21 19.0 22 19.5 23 29.0 24 58.0 25 78.0 26 106.5 27 158.0 28 210.0 29 239.0 30 296.0 31 378.0 32 520.0 33 637.0 34 741.0 35 996.0 36 1252.0 37 1357.0 38 1510.0 39 1890.0 40 2313.0 41 2695.5 42 2882.0 43 2986.0 44 3297.0 45 3473.5 46 3443.0 47 3470.5 48 3453.0 49 3253.5 50 3099.0 51 2946.5 52 2553.0 53 2171.5 54 2031.0 55 1891.5 56 1568.0 57 1261.0 58 1138.0 59 984.0 60 726.5 61 559.5 62 496.0 63 434.0 64 335.0 65 258.5 66 199.0 67 179.0 68 140.0 69 93.5 70 72.0 71 58.0 72 51.0 73 38.0 74 24.0 75 16.0 76 16.5 77 13.0 78 8.5 79 8.0 80 7.0 81 4.5 82 2.0 83 1.0 84 4.0 85 5.0 86 3.0 87 3.0 88 3.5 89 3.0 90 2.0 91 1.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 1.0 98 1.0 99 3.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6437421802478105 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 49554.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.15934132461557 #Duplication Level Percentage of deduplicated Percentage of total 1 84.50980392156863 64.3621100213908 2 8.757286698463169 13.338983734915447 3 3.2114467408585057 7.337450054486015 4 1.404345521992581 4.278161197885135 5 0.8664546899841018 3.2994309238406587 6 0.49019607843137253 2.2399806271945755 7 0.2649708532061473 1.4126003955281108 8 0.1669316375198728 1.0170722847802398 9 0.09803921568627451 0.6719941881583728 >10 0.2305246422893482 2.04221657182064 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004036001130080316 0.0 2 0.0 0.0 0.0 0.008072002260160633 0.0 3 0.0 0.0 0.0 0.008072002260160633 0.0 4 0.0 0.0 0.0 0.01009000282520079 0.0 5 0.0 0.0 0.0 0.01009000282520079 0.0 6 0.0 0.0 0.0 0.01009000282520079 0.0 7 0.0 0.0 0.0 0.01210800339024095 0.0 8 0.0 0.0 0.0 0.01210800339024095 0.0 9 0.0 0.0 0.0 0.014126003955281108 0.0 10 0.0 0.0 0.0 0.014126003955281108 0.0 11 0.0 0.0 0.0 0.016144004520321265 0.0 12 0.0 0.0 0.0 0.018162005085361425 0.0 13 0.0 0.0 0.0 0.018162005085361425 0.0 14 0.0 0.0 0.0 0.018162005085361425 0.0 15 0.0 0.0 0.0 0.02219800621544174 0.0 16 0.0 0.0 0.0 0.026234007345522056 0.0 17 0.0 0.0 0.0 0.028252007910562215 0.0 18 0.0 0.0 0.0 0.03430600960568269 0.0 19 0.0 0.0 0.0 0.03430600960568269 0.0 20 0.0 0.0 0.0 0.03430600960568269 0.0 21 0.0 0.0 0.0 0.03430600960568269 0.0 22 0.0 0.0 0.0 0.04036001130080316 0.0 23 0.0 0.0 0.0 0.04439601243088348 0.0 24 0.0 0.0 0.0 0.05045001412600396 0.0 25 0.0 0.0 0.0 0.05448601525608427 0.0 26 0.0 0.0 0.0 0.06457601808128506 0.0 27 0.0 0.0 0.0 0.07466602090648586 0.0 28 0.0 0.0 0.0 0.0968640271219276 0.0 29 0.0 0.0 0.0 0.10493602938208822 0.0 30 0.0 0.0 0.0 0.12713403559752998 0.0 31 0.0 0.0 0.0 0.16345804576825282 0.0 32 0.0 0.0 0.0 0.20381805706905598 0.0 33 0.0 0.0 0.0 0.25830407232514024 0.0 34 0.0 0.0 0.0 0.31682608871130485 0.0 35 0.0 0.0 0.0 0.3975461113129112 0.0 36 0.0 0.0 0.0 0.536788150300682 0.0 37 0.0 0.0 0.0 0.7224442022843767 0.0 38 0.0 0.0 0.0 1.0110182830851193 0.0 39 0.0 0.0 0.0 1.3359163740565847 0.0 40 0.0 0.0 0.0 1.7738224966702991 0.0 41 0.0 0.0 0.0 2.3146466481010615 0.0 42 0.0 0.0 0.0 2.883722807442386 0.0 43 0.0 0.0 0.0 3.4911409775194735 0.0 44 0.0 0.0 0.0 4.116721152681923 0.0 45 0.0 0.0 0.0 4.607095289986681 0.0 46 0.0 0.0 0.0 5.1378294385922425 0.0 47 0.0 0.0 0.0 5.567663558945797 0.0 48 0.0 0.0 0.0 5.99951567986439 0.0 49 0.0 0.0 0.0 6.471727812083787 0.0 50 0.0 0.0 0.0 6.834967913791016 0.0 51 0.0 0.0 0.0 7.295072042620172 0.0 52 0.0 0.0 0.0 7.944868224563103 0.0 53 0.0 0.0 0.0 8.758122452274286 0.0 54 0.0 0.0 0.0 9.43617064212778 0.0 55 0.0 0.0 0.0 10.003228800904065 0.0 56 0.0 0.0 0.0 10.57835896194051 0.0 57 0.0 0.0 0.0 11.07882310207047 0.0 58 0.0 0.0 0.0 11.569197239375226 0.0 59 0.0 0.0 0.0 12.00306736085886 0.0 60 0.0 0.0 0.0 12.477297493643299 0.0 61 0.0 0.0 0.0 12.935383621907414 0.0 62 0.0 0.0 0.0 13.423739758647132 0.0 63 0.0 0.0 0.0 14.0251039270291 0.0 64 0.0 0.0 0.0 14.602252088630586 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGGGT 15 0.002213213 69.992935 4 ACTTATT 15 0.002213213 69.992935 41 TACGGAC 15 0.002213213 69.992935 38 GATAAAG 20 0.006915798 52.4947 37 TTTACGG 20 0.006915798 52.4947 36 GTTTAGC 20 0.006915798 52.4947 11 TTATGAC 20 0.006915798 52.4947 50 ATATAGG 20 0.006915798 52.4947 4 TTACGGA 20 0.006915798 52.4947 37 CGATGTG 20 0.006915798 52.4947 18 TCGGTGG 20 0.006915798 52.4947 42 TGGTCGC 20 0.006915798 52.4947 46 GACTCCA 20 0.006915798 52.4947 42 GTATAAG 30 5.627199E-4 46.9939 1 GAGACAG 30 5.8277056E-4 46.661957 7 TATAGGC 30 5.8277056E-4 46.661957 5 AGATACC 35 0.001244795 39.995964 27 TAAGAGA 35 0.001244795 39.995964 4 CGCCCGA 35 0.001244795 39.995964 14 TGTAGAT 45 0.00427128 31.107973 34 >>END_MODULE