FastQCFastQC Report
Wed 25 May 2016
SRR1780360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49554
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT24764.996569399039432No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT23374.7160673204988495No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC7831.580094442426444No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC4380.8838842474875893No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG3080.6215441740323687No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT1990.40158211244299147No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1400.28252007910562216No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT1090.21996206158937723No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1080.2179440610243371No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1040.20987205876417644No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC840.16951204746337328No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT840.16951204746337328No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG800.16144004520321265No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT780.15740404407313235No Hit
CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC720.1452960406828914No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT680.13722403842273076No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT670.1352060378576906No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGTCTT640.12915203616257012No Hit
TTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC600.1210800339024095No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG550.11099003107720869No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTC150.002212327270.044
TATAACA301.0041913E-770.03
CTATACA1000.063.0000041
ATAACAC352.9178227E-760.04
ATTACAC200.006913039452.54
TAGTTAG200.006913039452.57
TTATAAC407.3386946E-752.52
CTTATAA407.3386946E-752.51
AGTCTTC200.006913039452.545
GATATGG200.006913039452.566
GTAGTTA200.006913039452.56
TTTACAC1550.049.6774182
CTTTACA1600.048.1251
TAACACA451.653123E-646.6666645
CCAGTCC305.824807E-446.6666644
TTACACA1750.042.03
ATATCGT459.075055E-538.88888537
GGGTACC400.00239723635.030
ATCGTAT601.1932647E-535.039
TATATCG450.00426917331.11110936
Produced by FastQC (version 0.11.5)